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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Spermatogonial stem cells,Lipofection,EGFP
- چکیده: در بالغین، سلولهای بنیادی اسپرماتوگونی (SSCs) تنها سلولهای بنیادی هستند که قادر به انتقال اطلاعات ژنتیکی به نسل بعد میباشند. با در نظر گرفتن این که یک SSC منفرد میتواند به تعداد زیادی از اسپرماتوزوآ تبدیل شود، دستکاری ژنتیکی این سلولها، یک تکنولوژی نوین با کاربردهای عملی در گونههای مختلف حیوانی را مهیا میسازد. در این مطالعه ارزیابی انتقال ژنEnhanced Green Fluorescent Protein (EGFP) به کلونیهای اسپرماتوگونی گاوی از طریق حامل لیپوفکتامین میباشد. کارایی انتقال ژن خارجی EGFP به SSCs از طریق حامل لیپوفکتامین در سه روز از شروع کشت کلونیهای اسپرماتوگونی (روز 4، 6 و 8) توسط میکروسکوپ فلورسنت ارزیابی شد. نتایج نشان داد که کلونیهای ترانسفکت شده از طریق لیپوفکتامین در هر سه روز انجام ترانسفکشن در مقایسه با گروههای شاهد به طور معنیداری افزایش یافت (P<0.05). کلونیهای ترانسفکت شده با لیپوفکتامین در مقایسه با گروههایی که تنها حاوی ژن خارجی بدون حامل بودند نیز بالاتر بود (P<0.05). بیشترین میزان کلونیهای ترانسفکت شده زمانی به دست آمد که ترانسفکشن در روز 4 کشت انجام شد. نتایج به دست آمده از این مطالعه پیشنهاد میکند که لیپوفکتامین میتواند به منظور انتقال مستقیم DNA خارجی به کلونی اسپرماتوگونی به ویژه در روز 4 کشت به صورت ایمن به کار رود.
- چکیده انگلیسی: Spermatogonial stem cells (SSCs) are the only stem cells in adults that can transfer genetic information to the future generations. Considering the fact that a single SSC gives rise to a vast number of spermatozoa, genetic manipulation of these cells is a potential novel technology with feasible application to various animal species. The aim of this study was to evaluate enhanced green fluorescent protein (EGFP) gene transfection into bovine SSCs via liposome carrier and assess the best incubation day in uptake exogenous gene by SSCs. Transfection efficiency of EGFP gene with lipofectamine 2000 was determined in days following each three day of transfection (day 4, 6 and 8 of the culture) by fluorescent microscope. Results showed that the transfected cells through lipofection increased significantly (P<0.05) in each three days of transfection in comparison with those of the control groups. The transfected SSCs were higher in comparison with those of the free exogenous gene carrier groups (P<0.05). In comparison with these three days, the rate of infected cells was higher when transfection proceeds at day four. It was concluded that lipofectamine can be used safely for direct loading exogenous DNA to SSCs particularly during the fourth day of culture.
- انتشار مقاله: 26-04-1395
- نویسندگان: P. Tajik,Kh. Hoseini Pajooh,Z. Fazle Elahi,G. Javdani Shahedin,H. Ghasemzadeh-Nava
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Sheep,Sperm,Transfection,Lipofection,SMGT
- چکیده: انتقال ژن به واسطه اسپرم میتواند یک روش ساده و ارزان برای تولید جانوران تراریخته باشد، اما کارایی این روش هنوز پایین است که میتواند به علت ورود مقدار ناکافی DNA خارجی به اسپرم باشد. هدف از این مطالعه بررسی روشهای تشدید کننده جذب DNA خارجی توسط اسپرم گوسفند مانند لیپوفکشن، انجماد سریع و تیمار با تریتون X100 و DMSO در میزان جذب و تحرک اسپرمهای دارای جذب بود. در آزمایش اول اسپرم گوسفند با کمپلکسی از مقادیر مختلف پلاسمید نشاندار شده با ماده فلورسنت رودآمین و لیپوفکتامین2000 گرمخانهگذاری شدند. در آزمایش دوم اسپرمها با تریتون X100 یا دی متیل سولفوکساید منجمد شدند. نتایج نشان داد که میزان ترانسفکشن در گروه لیپوزوم/DNA 100 نانوگرم کمتر از گروه بدون لیپوزوم بود اما در گروههای 300 و600 نانوگرم اختلاف معنیداری وجود نداشت. در شدت جذب و تحرک نیز اختلاف معنیداری بین گروههای مختلف وجود نداشت. همچنین هیچ کدام از اسپرمهای دارای جذب DNA خارجی، متحرک نبودند. لذا انتقال با واسطه لیپوزم باعث بهبود میزان ترانسفکشن نشد. همه اسپرمهای تیمار شده با تریتون X100 و منجمد شده پلاسمید نشاندار را به خود جذب کرده بودند اما همه آنها بی حرکت بودند. در اسپرمهای تیمار شده با دی متیل سولفوکساید با غلظت نهایی 1/0%، میزان جذب نسبت به گروه شاهد به طور معنیداری (P<0.05) بیشتر بود (40/69% در مقابل 80/57%). میزان تحرک در گروه تیمار و گروه شاهد اختلاف معنیداری نداشت. نتیجه آن که استفاده از لیپوفکتامین نتوانست نه میزان ترانسفکشن را بهبود بخشد و نه اسپرمهای ترانسفکت شده متحرک تولید کند. اگر تیمارهای تخریب کننده غشاء مثل انجماد و تیمار با تریتون X100 به هسته اسپرم صدمه نزنند، این اسپرمها میتوانند بدون نیاز به انتخاب جهت تزریق داخل سیتوپلاسمی اُاُسیت استفاده شوند.
- چکیده انگلیسی: Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and motility of sperms with plasmid uptake were evaluated. In the first experiment, ram sperms were incubated with a complex of rhodamine labeled plasmid (p-EGFP) and Lipofectamine 2000TM. In the second, spermatozoa were treated with Triton X-100TM or DMSO or were frozen without cryoprotectant. The results indicated that there was no significant difference (P<0.05) in the transfection rates and in the uptake intensity of lipofected sperms with 300 and 600 ng of plasmid in comparison with control group, i.e. transfected without lipofectamine. Furthermore, lipofection could not improve sperm motility during true plasmid uptake. Almost all of triton X100 treated and frozen-thawed spermatozoa had absorbed foreign DNA, though all were immotile. In spermatozoa treated with 0.1% DMSO, plasmid absorption rate (69.40%) was significantly higher (P<0.05) than untreated spermatozoa (57.80%), but sperm motility was not significantly different from control group. In conclusion, lipofectamine® 2000 could neither improve transfection rate, nor support motility in transfected sperms. The methods inducing membrane disruption like, freeze-thaw and triton X100 treatment, can be used in ICSI-sperm mediated gene transfer without the need for sperm selection, provided that they cause no damage to sperm nucleus.
- انتشار مقاله: 08-02-1394
- نویسندگان: K. Hoseini Pajooh,P. Tajik,M. Karimipoor,M. Behdani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Camel,Epididymal sperm,Protoplasmic droplets
- چکیده: بیضههای شترهای یک کوهانه در فصل تولید مثلی و غیر تولید مثلی در یک کشتارگاه محلی جمعآوری گردید. اسپرم از قسمتهای مختلف اپیدیدیم (سر، بدنه و دم) گرفته و به طور مجزا روی لام قرار داده شد، با ائوزین نیگروزین رنگآمیزی و با خشککن موی خشک شد و سپس به آزمایشگاه منتقل گردید. در آزمایشگاه لامها در زیر میکروسکوپ نوری جهت ارزیابی میزان اسپرمهای زنده و میزان اسپرمهای دارای قطره پروتوپلاسمی مورد بررسی قرار گرفتند. میزان اسپرمهای زنده 83، 90 و 86% در فصل تولید مثل و 80، 82 و 5/90% در فصل غیر تولید مثل برای قسمتهای سر، بدنه و دم اپیدیدیم بود، که اختلاف معنیداری را در بین آنها نشان نداد. همچنین هیچ اختلاف معنیداری بین درصد اسپرمهای زنده بیضههای راست و چپ وجود نداشت. میزان اسپرمهای زنده دارای قطره پروتوپلاسمی در فصل تولید مثل به ترتیب 66، 70 و 74% برای سر، بدنه و دم اپیدیدیم بود و در فصل غیر تولید مثل برای این قسمتها به ترتیب 73، 70 و 82% بود که از نظر آماری اختلاف معنیداری بین آنها وجود نداشت. همچنین اختلاف معنیداری بین بیضههای راست و چپ و بین فصلهای تولید مثل و غیر تولید مثل وجود نداشت. نتیجهگیری میشود که اسپرم را میتوان از هر قسمت بیضه به دست آورد.
- چکیده انگلیسی: Testicles were isolated from dromedary camels in a local slaughterhouse at breeding and non-breeding seasons. Sperms were recovered from different parts of the epididymis (caput, corpus and cauda) and stained separately on slide glasses by eosin nigrosin staining method and dried by a hair dryer and carried to the laboratory. In the lab, slides were observed for evaluation of the proportion of live sperms and the proportion of sperms with cytoplasmic droplets under a light microscope. The proportions of live sperm cells were 76.8,86.9 and 88.8% for caput, corpus and cauda epididymis, respectively. In the left testicle these values were 85.3, 83.1 and 88.4 for caput, corpus and cauda epididymis, respectively. No significant difference was also observed in the live sperm cells obtained from right and left testicles. The proportions of live sperm cells were 83, 90 and 86% in breeding and 80, 82 and 90.5% in non-breeding seasons for caput, corpus and cauda epididymis, respectively, which were not significantly different. The proportions of live sperms with protoplasmic droplets were 66, 70 and 74% in breeding and 73, 70 and 82% in non-breeding seasons for caput, corpus and cauda epididymis, respectively, which were not significantly different. The proportions of live sperms with protoplasmic droplets were significantly different neither among right and left testicles nor in different parts of epididymis. We concluded that sperm cells could be obtained from every part of the epididymis.
- انتشار مقاله: 02-09-1384
- نویسندگان: P. Tajik,M. R. Hassan-Nejad Lamsoo
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Fisheries Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: catfish,Radiography,Foreign body,Stones
- چکیده:
- چکیده انگلیسی: A red-tailed catfish (Phractocephalus hemioliopterus) referred to Aquatic Animal Health Department, Faculty of Veterinary Medicine, University of Tehran for showing lethargy, bottom sitting, extension of abdomen and severe abdominal injuries. Its abdomen was swollen and the bulk of the foreign bodies were easily palpated. The heaviness of foreign bodies had made swimming and buoyancy difficult for fish. Several radiographic images were taken after anaesthetizing the catfish with 10% carvacrol, 2 drops in 1 liter of water. Radiographs revealed some stones with their size, number and location. Stones were removed non-surgically through the large oral cavity using suitable forceps. Totally 16 stones and 2 shells were removed from the stomach of the fish. The catfish was then returned to the water where normal swimming and buoyancy was restored.
- انتشار مقاله: 12-10-1396
- نویسندگان: H.A. Ebrahimzadeh Mosavi,A.R. Vajihi,F. Hosseini,I. Sharifpour,P. Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Quantitative PCR,Virulence factors,Microwaves,Alpha-INT1 protein
- چکیده:
- چکیده انگلیسی: To date, registered users of mobile phone communication network exceeded from total numbers of the world population, while a little knowledge of the biological effects of, 900-1800 MHz microwave radiation, originating from the handsets or the base transceiver stations, have been released. The current study was designed for evaluation of 900-MHz radiation effects on Candida albicans proliferation, adherence and alpha-Int1 gene expression.
Candida albicans (ATCC:10231) grown in Yeast Peptone Dextrose (YPD) broth was distributed into five tubes (5 ml, 10^6 cells/ml) and exposed to 900 MHz GSM radiation for 6, 12, 18 and 24 hours, while the fifth tube was kept far from the radiation. Cell densities at 0, 6, 12, 18 and 24 hours were assayed (using turbidimetry in 600 nm). Equal cell densities (2.5 x 10^6 cells/ml, 200 ul) from exposed and unexposed yeasts were transferred into 96 well plates and incubated for 4 hours, in order to biofilm formation by the yeast. Yeast densities in biofilm network were assayed using the MTT method. Abundance of alpha-int1 mRNA was also estimated in the five yeast samples using q-RT-PCR method.
Microwave exposure led to increased proliferation rate and increased biofilm formation by the yeast and the effect was prominent in 18 hours exposed samples. Quantitative RT-PCR results showed significantly increased levels of the alpha-int1 mRNA in microwave exposed yeasts.
The significant increases in the yeast proliferation and biofilm formation after exposure to 900 MHz GSM radiation are partly mediated by changes in alpha-int1 protein expression.- انتشار مقاله: 26-07-1394
- نویسندگان: Ariyo Shahin-Jafari,Mansour Bayat,Mohammad Hassan Shahhosseini,Parviz Tajik,Shahla Roudbar-Mohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Diarrhea,case report,Child,Glucose-Galactose Malabsorption
- چکیده:
- چکیده انگلیسی: Introduction
Glucose-Galactose Malabsorption (GGM) is an autosomal recessive and rare disorder of intestinal transport of glucose and sodium-glucose cotransporter type (SGLT1).
Case Report
Our patient is a 32-day-old boy who was examined for severe diarrhea and acidosis and was treated with GGMdiagnosis. A number of laboratory tests were performed on this patient as well as positive test for reduced substance of stool and positive hydrogen breath test. On the other hand, the improvement of diarrhea with fasting and the initiation of a glucose and galactose free formula (fructose-basedformula [galactomin B-19]) was instructed. He was treated and followed with diagnosis of GGM.
Conclusion
In summary, careful clinical observation, laboratory tests, and the character of the external cues may provide indications of GGM.- انتشار مقاله: 01-08-1397
- نویسندگان: Pantea Tajik,Amir Hossein Goudarzian,Zeinab Pourzahabi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: children,Malnutrition,Nutritional model,F100 solution
- چکیده:
- چکیده انگلیسی: Background
The present study is aimed to develop a nutritional model for the prevention and treatment of malnutrition in children and its effectiveness in patients admitted to Mofid children's hospital.
Materials and Methods
This study was conducted as an interventional controlled clinical effectiveness trial with control group. The study population included children aged more than 1 year and under 10 years old who were admitted to the Mofid Children's Hospital in 2013-2014.
Results
In our study, boys and girls accounted for 50% and 50% of 300 children with malnutrition who were hospitalized for various reasons. All three groups were homogenous in terms of growth index,. After the use of the F100 solution, the growth index was improved and malnutrition severity, moderate malnutrition and mild malnutrition were improved by about 78%, 64% and 32%, respectively. In the nutrition counseling group, which had food orders and calorie intake, malnutrition severity was improved about 11%, 39% still had severe malnutrition and 60% had moderate malnutrition. There was a statistically significant relationship between the consumption of F100 solution and improvement of growth indices and serum albumin levels.
Conclusion
Children who are admitted to hospital for various reasons should be evaluated in terms of growth index so that their malnutrition is not exacerbated in the hospital. The use of the F100 solution that can easily be provided by families is an acceptable and accessible supplement for malnourished and underage children.- انتشار مقاله: 02-05-1396
- نویسندگان: Pantea Tajik,Mehdi Shadnoush
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Colitis,Pediatric,CMV
- چکیده:
- چکیده انگلیسی: Abstract
Cytomegalovirus (CMV) is a virus that can be consider as invasive infection after transplantation or chemotherapy, long-term corticosteroid users or in immunodeficient patients such as HIV. Different complications were seen in immunocompetent patients but colitis rarely occurs. The diagnosis of CMV was based on pathology by colonoscopy, positive CMV antigen and high CMV-IgM titer serum samples and a good response to systemic gancyclovir treatment. In this study we reported a 20 month girl with bloody diarrhea that her colonoscopy showed CMV ulceration.- انتشار مقاله: 24-11-1393
- نویسندگان: Farid Imanzade,Aliakbar Sayyari,Pantea Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Evaluation,case report,Immunoproliferative Small Intestine Disease
- چکیده:
- چکیده انگلیسی: Immunoproliferative small intestinal disease (IPSID) is the syndrome associated with Mediterranean lymphoma (a rare form of non-Hodgkin’s lymphoma). Many of the patients diagnosed with secretory IPSID have variable level of abnormal immunoglobulins in serum or other bodily fluids, identified as truncated alpha heavy chain globulins. Most cases are characterized by a loss of ability to synthesize light chains. As such, IPSID has been classified as a heavy chain disorder B-cell lymphoma. We present here the case of a 12-year-old boy admitted in our department for edema, abdominal pain and FTT, in whom we suspected the diagnosis of IPSID.
- انتشار مقاله: 15-12-1393
- نویسندگان: Farid Imanzadeh,Aliakbar Sayarri,Pantea Tajik
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Liver,Thioacetamide,Bone marrow,mesenchymal stem cell,Fibrosis
- چکیده:
- چکیده انگلیسی: The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of the treatment group were subdivided into two groups and sampled on the 4th and 6th weeks after BMSCs treatment. Fibrosis was induced by the intraperitoneal administration of 200 mg/kg of TA twice a week for a period of 14 weeks. All the animals underwent liver function tests and histopathologic evaluation 4 and 6 weeks after BMSCs transplantation. The BMSCs were characterized using osteogenic induction and reverse transcription-polymerase chain reaction. The BMSCs were plastic adherent, spindle-shaped, and positive for osteogenic differentiation. They expressed CD73 and were negative for CD45. The infiltration of inflammatory cells and deposition of collagen fibers were noticed after TA administration. A significant decline in inflammatory cells and a healing process were detected 4 weeks after cell transplantation. The amelioration in hepatic tissue was significant 6 weeks after cell therapy. Following the injection of BMSCs, a nonsignificant decrease was visible in aspartate transaminase level; however, this decline was significant for alanine aminotransferase level. The alkaline phosphatase and albumin levels showed an increasing trend after cell administration. The transplantation of BMSCs resulted in a significant regenerative effect after hepatic injuries. Therefore, it was shown that BMSCs transplantation can open a new window and be a therapy of choice in the amelioration of liver fibrosis.
- انتشار مقاله: 04-01-1396
- نویسندگان: D. Mehrabani,Z. Khajehahmadi,P Tajik,A. Tamadon,F. Rahmanifar,M. Ashraf,N. Tanideh,S. Zare
- مشاهده