در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Northern iran,Aspergillus,Aflatoxin,pattern
- چکیده:
- چکیده انگلیسی: Objective: Aflatoxin G1 and G2, are highly toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus fungi sampling was done according to "CBS" instructions from indoor and outdoor stations. Methods: In HPLC measuring, we first purified our sample of all Aflatoxins, We used imonoaffinty columns, Finally the device compared average of the results of the samples curve areas with average of the results of standard curve areas and showed the effective material value based on ppb. Geographical distribution of 107 mentioned samples and The frequency of subgenuses in different regions, The minimum, average and maximum amount of toxins, The average amount of produced toxin in culture medium were determined. Results: In comparison, the average of aflatoxin G1 and G2with HPLC method in biomass the highest examined amount is in the range of -0/5 -0/5 ppb which is produced by fungi such as A. niger, A. flavus, A. fumigatus. In biomass, the correlation between aflatoxin G1 and G2 measured by HPLC method isn’t any reverse relation and meaningful numeral differences. In culture medium, checking the average value of aflatoxin G1 and G2which is measured by HPLC method, we can see a correlation and there is a meaningful numeral difference.
- انتشار مقاله: 14-06-1394
- نویسندگان: Zohre Zohari Moafi,Arash Chaichi Nosrati,Omid Lakzaie Azar
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Aspergillus species,Citrinin,Toxigenic activity,Cell extracts,Food and Feed
- چکیده:
- چکیده انگلیسی: Objective: Citrinin is a mycotoxin produced by several species of the genera Aspergillus and Penicillium and occurs mainly in food products and animal feed. Some scientific reports show a link between Citrinin and nephrotoxic and possibly a carcinogenic effect for humans. Methods: Samples collected by settling plates,in northern Iran and pure culture isolation performed till the toxin measurement to be done in cell extracts (biomass) which prepared by merging culture in separated prepared culture media incubation. The amount of toxin measured by extracting solutions (50% Acetone, 25% Alcohol Methylic,25% Alcohol Ethylic and PBS washing proposed) using Direct Competitive ELISA. Results: In conducted research, the relative distribution per obtained Aspergillus species isolates for biomass preparation showed that the most frequent were A.ostianus, A.fumigatus,A.niveus,A.niger,A.awamori and A.parasiticus respectively with a prevalence (28.6%), according to averaged Citrinin, indicated that the potent species was A.niger (2009.3ppb) whereas the lowest observed by A.wentii (18.46 ppb).According to maximum Citrinin limits (200ppb) in food products and animal feed including especially related to the Aspergilli and Penicilli, as the result of our measurements and the performed statistical analysis , the maximum amount of Citrinin in the cell extracts, respectively were determined (2009.3ppb) and always produced by A.niger , that were too much more than the universal and local standards allowance enable us to introduce it for all biotechnologic or toxicologic research mentions eventhought as a scientific opinion on the risks for public and animal health related to the presence of Citrinin in food and feed at the targeted geographic area.
- انتشار مقاله: 23-06-1393
- نویسندگان: Pouya Nejati,Arash Chaychi Nosrati,Mansour Bayat,Omid Lakzaie Azar
- مشاهده