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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Physics
- نوع مقاله: Journal Article
- کلمات کلیدی: Glioma,volumetric modulated arc therapy,Computer-Assisted Radiotherapy Planning,Three-Dimensional Conformal Radiotherapy
- چکیده:
- چکیده انگلیسی: Introduction: We aimed to dosimetrically compare three-dimensional conformal radiotherapy (3D-CRT) and volumetric modulated arc therapy (VMAT) in terms of planning target volume (PTV) coverage, organ at risk (OAR) sparing, and conformity index (CI).
Material and Methods: Planning data of 26 high grade glioma (HGG) patients were used. Prescribed dose for 3D-CRT was 46Gy in 23 fractions to low-risk PTV (LR-PTV) and 14 Gy in 7 fractions to high-risk PTV (HR-PTV). VMAT plans were conducted using 46 Gy in 30 fractions to LR-PTV and 60 Gy in 30 fractions to HR-PTV.
Results: Tumor locations were frontal, parietal, temporal, and multi-lobed in 27%, 15%, 23%, and 35% of cases, respectively. Histology was glioblastoma multiform in 89% of patients. Mean values of PTV D95 (dose received by 95% volume) in 3D-CRT and VMAT were 96.6% and 98.8% for the LR-PTV and 97.3% and 99% for HR-PTV (p <0.001), respectively. Mean values of CI in 3D-CRT were 0.96 and 0.97 for LR-PTV and HR-PTV and 0.98 and 0.99 for LR-PTV and HR-PTV of VMAT (both p <0.001), respectively. Mean Dmax of right optic nerve (maximum point dose received by the organ) for 3D-CRT and VMAT were 31.59 and 25.57Gy (P=0.02). Mean Dmax for left optic nerve and optic chiasm were 28.81 and 22.14 Gy (P=0.019) and 42.24 and 37.12 Gy (P=0.055) respectively for 3D-CRT versus VMAT. Doses to other OARs were not statistically different between 3D-CRT and VMAT.
Conclusion: VMAT achieved better coverage of the PTV and delivered fewer doses to bilateral optic nerve and chiasm.- انتشار مقاله: 23-08-1397
- نویسندگان: Harikesh Bahadur Singh,Ajeet Kumar Gandhi,Shantanu Sapru,Rohini Khurana,Rahat Hadi,Sambit Swarup Nanda,Satyajeet Rath,Avinav Bharati,Anoop Kumar Srivastava,Surendera P Mishra,Kamal Sahni,Nuzhat Husain,Madhup Rastogi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: DNA damage,Q-PCR,Low Dose Radiation (LDR),DNA Lesions
- چکیده:
- چکیده انگلیسی: Background: Radiation causes oxidative lesions and strand breaks in DNA of exposed cells. Extended length
PCR is a reliable method for assessing DNA damage. Longer DNA strands with DNA damage are difficult to amplify
compared to smaller DNA strands by PCR. The present study was aimed to evaluate DNA damage caused by ionising
radiation exposure in therapeutic and diagnostic medicine. Materials and Methods: The study group comprised 50
cases with low dose single exposure (LDS), low dose multiple exposure (LDM) and low dose angiography (LDA)
which were compared with 25 high dose controls (HDC) and 25 controls with no exposure (NEC). Blood samples were
collected within 1 hour of radiation exposure. DNA was isolated using a kit based protocol, 50 ng aliquots of DNA
were used to amplify a long 13kbp DNA fragment of the β-actin gene by conventional PCR and band intensity was
then quantified. Relative amplification was calculated and damage was expressed in terms of lesions per kilobase (kbp)
by assuming a Poisson distribution. Result: Relative amplification was found to be 1.0, 0.87, 0.86, 0.72 and 0.69 with
NEC, LDS, LDM, LDA and HDC groups, respectively. Cases undergoing angiography as well as high dose controls
had high values, compared to NEC. The lesions/kbp calculated for LDS was 0.13, for LDM 0.15, for LDA 0.32 and
for HDC 0.37 suggesting a linear increase in quantity with increasing radiation dose. Conclusion: DNA damage, even
at low doses of radiation can be assessed by quantitative extra long PCR.- انتشار مقاله: 26-10-1396
- نویسندگان: Kainat Khan,Shikha Tewari,Madhup Rastogi,Gaurav Raj Agarwal,Surendra Prasad Mishra,Nuzhat Husain
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Survival,Glioblastoma,Nanog,CD24
- چکیده:
- چکیده انگلیسی:
Background and aim: Glioblastoma (GBM) is one of the most common and aggressive brain tumors with a median survival of 12-14 months. The aim of present study was to evaluate the gene expression profile of stem cell markers Nanog and CD24 in GBM and to determine its relationship to outcome in terms of treatment response and overall survival. Material and methods: This was a retrospective as well as retrospective study which included 51 histologically confirmed cases of GBM. Expression of CD24, and Nanog was evaluated by RT-PCR. Control tissue included debrided brain tissue from open head injury cases. All cases of GBM underwent total surgical resection and subsequently chemotherapy. Immediate treatment response was evaluated at 3 months using Response Evaluation Criteria In Solid Tumors (RECIST) guidelines and overall survival was measured at 36 months. Result: As compared to control gene, expression of CD24 and Nanog was seen to be unregulated to 24.5% and 31.7% respectively. However, the difference in mean expression of cases and controls was not statistically significant. Correlation between expressions of these two markers was also not statistically significant. On univariate cox regression analysis, cases with >2 fold expression of CD24 and Nanog had significantly poor survival as compared to those with 2 fold CD24 expression had a statistically significant correlation with poor survival. Conclusion: An overexpression of CD24 by more than two fold was associated with poor overall survival in GBM. Poor survival may be related to increased "stemness" of tumour cells. Targeted therapy inclusive of drugs targeting stem cells directly or indirectly may be a promising therapeutic option.- انتشار مقاله: 24-02-1396
- نویسندگان: Priyanka Soni,Sumaira Qayoom,Nuzhat Husain,Praveen Kumar,Anil Chandra,Bal Krishna Ojha,Rakesh Gupta
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: : Liquid Based Cytology (LBC),Flowcytometry (FCM),Low grade Squamous Intraepithelial Lesion (LSIL),High grade Squamous Intraepithelial Lesion (HSIL),Squamous cell carcinoma (SCC)
- چکیده:
- چکیده انگلیسی:
Background: DNA ploidy analysis of cervical intraepithelial neoplasia (CIN) and invasive cervical cancer samples by flow cytometry (FCM) has been established as an aid to prognostic assessment. Liquid based cytology (LBC) increases diagnostic specificity by using ancillary techniques that provide information beyond morphology. The present study was undertaken to assess DNA ploidy in LBC samples as an adjunct for early detection of cervical pre-cancer and cancer. Methods: DNA ploidy assessment was performed on LBC samples of 50 cases and 31 controls. Cell pellets were obtained by centrifugation and stained with Telford reagent. At least 20,000 R1 gate (G0-G1) events were acquired on a BD FACSCalibur by using a 575±10 nm filter. Results: Mean diploid G1 values were lowered significantly (p<0.01) while diploid S values were significantly elevated (p<0.01) in both high grade squamous intraepithelial lesions (HSILs) and squamous cell carcinomas (SCCs) as compared to controls. Receiver operating curve (ROC) analysis of the diploid G1 value was found to have significant diagnostic potential (AUC=0.682, Z=2.00, p=0.046) for distinction between control and low grade squamous intraepithelial lesion (LSIL) at a cut off value of ≤91.6 with a sensitivity and specificity of 50.0 and 87.1%, respectively. Conclusions: ROC analysis of diploid G1 and diploid S values allows discrimination between LSIL and HSIL with sensitivities and specificities of 65 and 100% and 70 and100%, respectively, and between LSIL and SCC cases with values of 71.4 and 100% and 64.3 and 100%, respectively.- انتشار مقاله: 09-01-1396
- نویسندگان: Sridhar Mishra,Namrata Awasthi,Nuzhat Husain,Akansha Anand,Yahodhara Pradeep,Roohi Ansari,Sarita Saxena
- مشاهده