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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,Gastric cancer,immunohistochemistry,Lynch syndrome
- چکیده:
- چکیده انگلیسی: Colorectal cancer (CRC) is one of the most frequent neoplasms worldwide, and up to 15% have a family history. Lynch syndrome (LS) is a hereditary cause of CRC and gastric (GC). Individuals with LS have mutations in mismatch genes repair. p53, cyclin D1, β-catenin, APC and c-myc proteins are involved in the cell cycle and carcinogenesis. Objective: To study the expression of p53, Cyclin D1, β-catenin, APC and c-myc proteins in patients with CRC and GC with at least one of the Bethesda positive criteria. Compare the expression of these proteins with the presence or absence of expression of the DNA repair proteins. Patients and Methods: We included 70 individuals with CRC or GC with at least one of the Bethesda positive criteria. Protein expression of MLH1, MSH2, MSH6, PMS2, p53, cyclin D1, β-catenin, APC and c-myc were analized by immunohistochemistry tumours tissues. Results: Deficient expression of MLH1, MSH2, MSH6 and PMS2 were respectively 38.7%; 17.7%; 26.22% and 48.38%. We found a negative association between deficiency of PMS2 and age, and positive association between PMS2 deficiency and APC positive. The positive imunoexpression of APC increases by 4 times the chance of having deficiency of PMS2. Conclusions: Patients with loss of expression of PMS2 had a higher risk of mutation or deletion of APC and tumours with positive immunoexpression of cyclin D1 had an increased risk of loss of expression of MSH2. These results suggest that tumours with loss of expression of DNA repair proteins had a higher loss of cell control cycle.
- انتشار مقاله: 06-01-1398
- نویسندگان: Tais Fernanda Marcolino,Celia Aparecida Marques Pimenta,Ricardo Artigiani Neto,Paula Castelo,Marcelo Souza Silva,Nora Manoukian Forones,Celina Tizuko Fujiyama Oshima
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,Biomarker,Lipidomic
- چکیده:
- چکیده انگلیسی: Backgrounds: Colorectal (CRC) is one of the main cause of cancer worldwide. The search for noninvasive markers
for diagnosis and monitoring as the use of analytical technologies such as mass spectrometry (MS), which allowed the
search for lipid metabolites as candidates for probable biomarkers are needed. Objective and Methods: The objective
was to establish the lipid profile of patients with locally advanced, unresectable or metastatic CRC. Peripheral blood
was collected from patients with CRC and controls with normal colonoscopy. After lipid extraction, the samples were
processed and analyzed in the MALDI TOF / TOF equipment. From the data matrix, the statistical analyzes were
performed by the principal component analysis methods and the least squares discriminant analysis. The importance of
the variable in the projection was used to identify the ions that had the greatest discriminatory effect between the groups.
Results: Eight lipids were identified as potential biomarkers and a multiple logistic regression model was proposed
to calculate the performance of the test where we observed values of AUC 0.87, sensitivity 88.33% and specificity
83.78% and for a validation test with 1,000 permutations a p glycerophospholipids and policetidis. The strength of the association between the peak intensities of these lipids and
the presence of CRC make these metabolites candidates for possible biomarkers. The sphingolipid (m / z = 742.98869)
could be a biomarker in monitoring patients with CRC. In the survival analysis, three lipids showed a prognostic value
for colorectal cancer, sphingolipid (m / z = 857.11525) and policetidis (m / z = 876.20796) and glycerophospholipid
(m / z = 1031.54773).- انتشار مقاله: 30-07-1396
- نویسندگان: Adiel Goes De Figueiredo Junior,Patrícia Valéria Pereira Serafim,Augusto Azzolini De Melo,Aledson Vitor Felipe,Edson Guimarães Lo Turco,Ismael Dale Cotrim Guerreiro Da Silva,Nora Manoukian Forones
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Drug resistance,Gastric cancer,RNA interference,ABCB1,epirubicin
- چکیده:
- چکیده انگلیسی: Background: Gastric cancer is one of the most common malignancies worldwide. Epirubicin (EPI) is used
extensively in the treatment of multiple cancers despite its tendency to induce multidrug resistance though overexpression
of the ABCB1 efflux pump. However, this overexpression can be disrupted using short interfering RNAs (siRNAs).
Objective and Methods: The aim of this study was to explore approaches to reverse EPI resistance and thus increase
the success of chemotherapy treatment in an EPI-resistant gastric cancer cell subline (AGS/EPI). Methods: The study
focused on effects of ABCB1 knockdown by siRNA technology using TaqMan gene expression assays with quantitative
real-time reverse-transcription PCR (qRT-PCR). MTT assays were performed to evaluate viability and prolifer in subline.
ABCB1 protein localization and EPI intracellular fluorescence intensity in AGS/EPI cells were detected by confocal
microscopy. Results: The siRNA efficiently downregulated ABCB1 mRNA in AGS/EPI cells. Thus MDR reversal
was clearly demonstrated in the AGS/EPI cells, offering the possibility of future in vitro chemoresistance assays for
the GC field. Conclusions: ABCB1 knockdown decreased EPI efflux and increased EPI sensitivity in AGS/EPI cells.
This result provides a novel strategy for targeted gene therapy to reverse EPI resistance in gastric cancer.- انتشار مقاله: 18-04-1396
- نویسندگان: Aledson Vitor Felipe,Juliana De Oliveira,Andrea Aparecida Moraes,Jerônimo Pereira De França,Tiago Donizetti Da Silva,Nora Manoukian Forones
- مشاهده