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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: AML,GM-CSF,CEBPA,FLT3-ITD,NPM1 mutation A
- چکیده:
- چکیده انگلیسی: Background: Acute myeloid leukemia (AML) is characterized by clonal expansion of myeloid precursors with diminished capacity for differentiation. It develops as the consequence of a series of genetic changes in a hematopoietic precursor cell. Purpose This study aimed to investigate the correlation between GM-CSF gene expression and different molecular prognostic markers such as FLT3-ITD, NPM1 mutation A and CEBPA gene expression in 100 Egyptian AML patients. As well as, correlation with the response to induction therapy, DFS andOS in these patients. Methodology: Quantitative assessment of GM-CSF gene expression was performed by qRT-PCR. Additional prognostic molecular markers were determined as FLT3-ITD and NPM1 mutation A together with quantitative assessment of CEBPA gene expression by qRT-PCR. Results: Patients with high GM-CSF expression levels had better OS and DFS with p value 0.004 and 0.02, respectively. However, no statistically significant difference between low andhigh GM-CSF gene expression was found regarding the response to therapy (p value= 0.08). Most patients with low CEBPA expression had resistant disease together with poor OS and DFS (P value =
- انتشار مقاله: 18-11-1398
- نویسندگان: Bassant Nagdy,Hebatallah A Kassem,Abdel-Rahman B Abdel-Ghaffar,Dina M Seoudi,Neemat M Kassem
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: acute myeloid leukemia,Chemoresistance,Treatment outcome
- چکیده:
- چکیده انگلیسی: Background: Acute Myeloid Leukemia (AML) is a heterogeneous disorder with variable genetic abnormalities and
cytogenetic alterations which provide a significant disease prognosis and determine response to therapy. Purpose: We
aim to investigate the expression of the MDR1 gene in 100 Egyptian AML patients, to identify their role on both the
progression and chemotherapeutic refractoriness together with assessment of known prognostic molecular markers;
FLT3-ITD and NPM1 mutations. Methodology: Quantitative assessment of MDR1 gene expression was performed
by quantitative RT-PCR. Additional prognostic molecular markers were determined as internal tandem duplications of
the FLT 3 gene and nucleophosmin gene mutation A. Results: MDR1 gene expression levels and FLT3/ITD mutations
were significantly higher in AML patients with resistant disease with P value NPM1 was insignificantly higher in patients with CR P-value 0.14. In MDR positive group, wild FLT3/ITD with or
without NPM1 mutation was favorable in achieving CR with p value 0.02. MDR negative group, wild FLT3/ITD with
or without NPM1 mutation showed insignificantly higher CR rates with p value (0.35). Kaplan-Meier curves revealed
statistically significant difference between MDR1-negative and MDR1-positive patients regarding their DFS and OS
between the two groups where DFS and OS were higher in MDR1-negative patients with p value 0.004 and 0.01,
respectively. Conclusion: The results obtained by the current work together with the previous researches concerning
the study of multidrug resistance genes in AML patients provide additional evidence of the role played by these genes
as predictors of chemoresistance and poor treatment outcome.- انتشار مقاله: 27-01-1398
- نویسندگان: Neemat M Kassem,Nashwa Medhat,Hebatallah A Kassem,Mohamed A El-Desouky
- مشاهده