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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Leishmania major,Leishmaniasis,Metabolomics,Leishmania tropica,Proton magnetic resonance spectroscopy
- چکیده:
- چکیده انگلیسی: Background: Cutaneous leishmaniasis caused by Leishmania species (L. spp) is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between L. spp, we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of L. major and L. tropica by proton nuclear magnetic resonance (1H-NMR).
Methods: L. tropica and L. major were collected from three areas of Iran, namely Gonbad, Mashhad, and Bam, between 2017 and 2018, and were cultured. The metacyclic promastigote of each species was separated, and cell metabolites were extracted. 1H-NMR spectroscopy was applied, and the data were processed using ProMatab in MATLAB (version 7.8.0.347). Multivariate statistical analyses, including the principal component analysis and the orthogonal projections to latent structures discriminant analysis, were performed to identify the discriminative metabolites between the two L. spp. Metabolites with variable influences in projection values of more than one and a P value of less than 0.05 were marked as significant differences.
Results: A set of metabolites were detected, and 24 significantly differentially expressed metabolites were found between the metacyclic forms of L. major and L. tropica isolates. The top differential metabolites were methionine, aspartate, betaine, and acetylcarnitine, which were increased more in L. tropica than L. major (p <0.005), whereas asparagine, 3-hydroxybutyrate, L-proline, and kynurenine were increased significantly in L. major (p <0.01). The significantly altered metabolites were involved in eight metabolic pathways.
Conclusion: Metabolomics, as an invaluable technique, yielded significant metabolites, and their biochemical pathways related to the metacyclic promastigotes of L. major and L. tropica. The findings offer greater insights into parasite biology and how pathogens adapt to their hosts.- انتشار مقاله: 28-03-1398
- نویسندگان: Fatemeh Tabrizi,Seyyed Javad Seyyed Tabaei,Nayeb Ali Ahmadi,Afsaneh Arefi Oskouie
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: oral cancer,Biomarker,Protein-protein interaction,hub-bottleneck,Cluster
- چکیده:
- چکیده انگلیسی: Background: Oral cancer is a frequently encountered neoplasm of the head and neck region, being the eight most
common type of human malignancy worldwide. Despite improvement in its control, morbidity and mortality rates have
improved little in the past decades. Therefore, prevention and/or early detection are a high priority. Proteomics with
network analysis have emerged as a powerful tool to identify important proteins associated with cancer development
and progression that can be potential targets for early diagnosis. In the present study, network- based protein- protein
interactions (PPI) for oral cancer were identified and then analyzed for use as key proteins/potential biomarkers.
Material and Methods: Gene expression data in articles which focused on saliva proteomics of oral cancer were
collected and 74 candidate genes or proteins were extracted. Related protein networks of differentially expressed proteins
were explored and visualized using cytoscape software. Further PPI analysis was performed by Molecular Complex
Detection (MCODE) and BiNGO methods. Results: Network analysis of genes/proteins related to oral cancer identified
kininogen-1, angiotensinogen, annexin A1, IL-8, IgG heavy variable and constant chains, CRP, collagen alpha-1 and
fibronectin as 9 hub-bottleneck proteins. In addition, based on clustering with the MCODE tool, vitronectin, collagen
alpha-2, IL-8 and integrin alpha-v were established as 5 distinct seed proteins. Conclusion: A hub-bottleneck protein
panel may offer a potential /candidate biomarker pattern for diagnosis and treatment of oral cancer disease. Further
investigation and validation of these proteins are warranted.- انتشار مقاله: 28-09-1396
- نویسندگان: Nasrin Amiri Dash Atan,Mehdi Koushki,Mostafa Rezaei Tavirani,Nayeb Ali Ahmadi
- مشاهده