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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: In vitro culture, Lysophosphatidic acid, Lysophosphatidic acid receptor, Mouse, Ovary
- چکیده:
- چکیده انگلیسی: Lysophosphatidic acid (LPA) known as a serum-derived growth factor, is involved in several cell physiological functions in the female reproductive system including: oocyte maturation, in vitro fertilization and embryo implantation by its transmembrane G protein-coupled receptors. The aim of the present study was to examine the effect of LPA on in vitro follicular development of mouse ovarian tissue. Neonatal mouse ovarian tissues were cultured in five different concentrations of LPA (0, 5, 10, 20 and 40 µM). The developmental competence and the function of cultured ovarian tissue were assessed by morphological study using hematoxylin and eosin staining and hormonal analysis. The expression of LPA receptor (LPAR 1-4) genes were analyzed by real-time RT-PCR. The proportion of preantral follicles and the level of E2 hormone were significantly higher in the 20 µM LPA-treated group than those in the other treatment groups. There was a significant difference in the expression of LPAR 1-4 genes in 20 µM LPA treated group in comparison with 0 µM LPA (control group) treated and non-cultured groups. In addition, the expression of LPAR1 gene was higher than other receptor genes in all studied groups. In conclusion supplementation of the media with 20 µM LPA, could improve the survival and developmental potential of follicles and it had positive effects on cell function and stimulation of E2 synthesis in mouse whole ovarian tissues.
- انتشار مقاله: 24-02-1396
- نویسندگان: Neda Abedpour,Mojdeh Salehnia,Nassim Ghorbanmehr
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Real Time PCR,Biofilm,P. aeruginosa,microtiter plate,Scanning Electron Microscopy (SEM)
- چکیده:
- چکیده انگلیسی: Background:
Microorganisms attach to various surfaces and they have manufactured biofilms by production polysaccharides like PSL in P.aeruginosa. synthesis of this kind of polysaccharide has done by PSL gene cluster. The aim of this study is consideration biofilm formation which is one of the major cause antibiotic resistance
Methods:
In this study, 100 P. aeruginosa were isolated with bacteriological and biochemical methods and pslA gene detection with PCR in all of the P. aeruginosa isolated from patients Then biofilm formation checked with microtiter plate method and it showed with SEM. Finally, expression of main attachment gene pslA in 6 strains could make moderate and strong biofilm were investigated by real-time PCR assay.
Results:
In this study, 100 P. aeruginosa were isolated that these strains showed High rates of MDR. The presence pslA gene in all of the pseudomonas isolated from patients was proven. Microtiter plate method showed 24 (24%) strains could make biofilm Among 100 strains that showed with SEM. The pslA expression in strains which making moderate and strong biofilms are more than other strains
Conclusions:
Hence, for bacterial biofilm treatment is recommended: Before antibiotics are prescribed, biofilm formation by bacteria should be investigated.- انتشار مقاله: 16-03-1398
- نویسندگان: Mohammad Abootaleb,Mohammad Reza Zolfaghari,Nazila Arbab Soleimani,Nassim Ghorbanmehr,Mohammad Reza Yazdian
- مشاهده