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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Dentistry, Shiraz University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Statement of the Problem: Oral squamous cell carcinoma is the most common oral malignancy. Toll-like receptor (TLR) activation led to alterations in the levels of mRNA encoding the TLR accountable for recognizing the inducing agonist and cross-regulation of other TLR.Purpose: The purpose of this study is determination of mitogen-associated protein kinase (MAPK) activation in human immortalized oral epithelial cell (HIOEC) line via up regulating of TLR7.Materials and Method: expression of TLR7 was measured in HIOEC and normal cells by quantitative real-time polymerase chain reaction (qRT-PCR) and samples were calibrated by β-actin.Results: Western blot analysis discovered high expression of TLR7 and MAPK in HIOEC cell lines. TLR7 was over-expressed in HIOEC cell line. Imiquimod-induced expression of interleukin (IL)-6, IL-8, and vascular endothelial growth factor (VEGF) was inhibited by TLR7 siRNA in HIOEC cells as determined by reverse transcription polymerase chain reaction (RT-PCR). Mean fluorescence intensity of nuclear p38 expression was determined in HIOEC cell lines (p< 0.05). RT-PCR analysis of IL-6, IL-8, and VEGF mRNA expression in HIOEC cells stimulated with imiquimod (1 μg/ml) for indicated time points.Conclusion: TLR7 is functionally over-expressed in HIOEC cell line of oral squamous cell carcinoma and development of resistance to cisplatin in human oral squamous cell carcinoma might occur through the mechanism involving activation of TLR7 and its dependent signaling pathway. Key Words Toll-Like Receptor 7; Mitogen-Activated Protein Kinase; Squamous Cell Carcinoma;
- انتشار مقاله: 17-11-1395
- نویسندگان: Ali Mahmoud Hashemi,Hamid Mahmoud Hashemi,Shiva Solahaye Kahnamouii,Talieh Mahmoud Hashemi,Hassan Agajani,Khalil Frozannia,Alireza Pournasrollah,Rasoul Sadeg,Rasoul Estakhri,Ebrahim Razmpa,Naghmeh Bahrami
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Dentistry, Shiraz University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Statement of the Problem: Evidence shows thiabendazole has the potential to inhibit angiogenesis in melanoma and fibrosarcoma; however, its effect on oral squamous cell carcinoma has not been previously studied.Purpose: This study sought to assess the cytotoxic effects of thiabendazole on HN5 head and neck squamous carcinoma cell line.Materials and Method: HN5 cell lines were exposed to different concentrations of thiabendazole (prepared from 99% pure powder) for 24, 48 and 72 hours. Cell viability was assessed by the methyl thiazol tetrazolium assay, and IC50 of thiabendazole was calculated. Cells were also exposed to different concentrations of thiabendazole for 48 hours to determine its effect on expression and transcription of vascular endothelial growth factor gene. Expression of vascular endothelial growth factor mRNA was assessed by real-time polymerase chain reaction. The vascular endothelial growth factor release was assessed by the enzyme-linked immunosorbent assay test.Results: In all concentrations of thiabendazole except for 200 and 550μM, cell viability was significantly different at different time points (p< 0.05). At 48 and 72 hours, cell viability at all concentrations of thiabendazole (100-650μM) significantly decreased compared to the control group (zero concentration). In addition, cell viability significantly decreased with an increase in thiabendazole concentration. At 48 hours, expression of vascular endothelial growth factor mRNA was significantly lower in presence of 500μM thiabendazole compared to the control group (p< 0.001) and release of vascular endothelial growth factor was inhibited in a dose-dependent manner.Conclusion: Thiabendazole inhibited the proliferation of HN5 cells in a dose-dependent and time-dependent manner. It also inhibited the expression of vascular endothelial growth factor gene. Keywords ● Carcinoma ● Squamous Cell ● Vascular Endothelial Growth Factor ● Angiogenesis Inhibitors ● Thiabendazole
- انتشار مقاله: 13-05-1395
- نویسندگان: Amir Jalal Abassi,Abdolreza Mohamadnia,Seyed Alireza Parhiz,Nahid Azizi Moghadam,Naghmeh Bahrami
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Lung cancer,Biomarker,LUNX,CK19,CEA
- چکیده:
- چکیده انگلیسی: Introduction: Lung cancer is the most common cause of cancer-related death among males and females. The
diagnosis of lung cancer is of great importance for clinical considerations and follow-up treatment. This study aimed
to examine the expression of CEA, LUNX, and CK19 biomarkers in the cancerous and healthy tissues of patients
suffering from NSCLC. Methods: In this study, 30 patients with NSCLCs referring to Masih Daneshvari Hospital
in Tehran were voluntarily selected prior to taking any treatment. A tissue sample from the center and a sample of
healthy tissues close to the cancerous masses were prepared by a specialist in the bronchoscopy sector and tested using
real-time RT-PCR. Results: Positive CEA mRNA was observed in cancerous tissues in the center of tumors of 25 out
of 30 cases. In the healthy tissue group, the same was found in 10 out of 30 cases (P<0.001). The markers CK19 and
LUNX mRNAs showed to be positive in cancerous samples in the center of tumors of 15 and 22 out of 30 cases, and in
the healthy tissue group, the expression was observed in 5 and 4 out of 30 cases, respectively(P<0.001). Conclusion:
This study confirms that the aformentioed markers are the ones with a relatively appropriate sensitivity and specificity
for the diagnosis of lung cancer.- انتشار مقاله: 20-07-1397
- نویسندگان: Hamidreza Jamaati,Adnan Khosravi,Atefeh Abedini,Arda Kiani,Payam Tabarsi,Hossein Dargahi,Naghmeh Bahrami,Mehdi Kazempour Dizaji,Farahnaz Sadegh Beigee,Mihan Pourabdollah,Abbas Gheysouri,Nasser Ahmadian,Masoum Abniki,Abdolreza Mohamadnia,Aliakbar Velayati
- مشاهده