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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Ultrasonography,Sheep,Flaxseed,OVULATION,Reproduction
- چکیده:
- چکیده انگلیسی: The purpose of this study was to compare the effects of using different levels of flaxseed in diets on the reproductive performance of estrous-synchronized Baluchi ewes (para 3). Diets contained either basal diet (control) or different levels of extruded flaxseed (2%, 5%, 7%, 10%, and 12%) and were fed from lambing to 60 days after lambing. The ewe estrus cycles were synchronized using controlled internal drug release (CIDR) for 14 days starting from day 16 of fat supplementation. The rams were introduced 24 h after CIDR removal. The ewes fed control diets had the highest mean dry matter intake (1,800±35 g) which was declined with the increase of flaxseed levels. The experimental diets exerted no effects of urea concentration in blood plasma. However, plasma glucose concentration was lower (p <0.05) in the ewes fed the control diet and 2% flaxseed, compared to those in other groups. Nonetheless, there was no difference among the ewes fed 5%, 7%, 10%, and 12% flaxseed in terms of plasma glucose concentrations (p <0.05). The ewes fed 2% flaxseed had the highest level of plasma triglyceride concentration among other groups. In addition, the control group had the lowest level of plasma total cholesterol and low-density lipoproteins concentration in comparison to other groups (p <0.05). However, plasma nonesterified fatty acids and β-hydroxybutyrate concentrations were similar among the groups (P>0.05). The mean interval between CIDR removal and the exhibition of estrus ranged from 30 to 40 h with the shortest interval being recorded in the ewes fed 12% flaxseed (p <0.05). The control group had the lowest number of follicles on estrus day among other groups (p <0.05). Furthermore, the ewes fed 10% and 12% flaxseed had the highest ovulation, pregnancy, and lambing rates, compared to other groups (p <0.05). In conclusion, the findings revealed that feeding the ewes with 10% and 12% flaxseed resulted in the improvement of reproductive performance.
- انتشار مقاله: 20-11-1398
- نویسندگان: M. Didarkhah,M. Vatandoost,E. Dirandeh,N. Dadashpour Davachi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Electrosurgery,Bovie Machine,Vasectomy,Embryo transfer
- چکیده:
- چکیده انگلیسی: Vasectomy in laboratory animals is a crucial step in the production of surrogate female mice. The surrogate mothers play a key role in successful embryo transfer, most important steps for the production of transgenic animal models, investigation of the preimplantation embryo development, and revitalization of cryopreserved strains. Abdominal and scrotal surgeries are common surgical procedures used in routine veterinary practice to produce vasectomized males. Two different surgical practice, namely electrosurgery and cold surgical practice, have been used as common techniques in operating rooms. Based on current knowledge, there is no published “technical note” as a detailed and step by step guideline to describe vasectomy using an electrosurgery machine (i.e., Bovie machine) in laboratory animal research and breeding facilities.The common problem during the laboratory animal surgery would be animal mortalities as a consequence of profound bleeding. The use of Bovie machine leads to the prevention of profound bleeding during the surgical practice.
- انتشار مقاله: 07-01-1398
- نویسندگان: N. Dadashpour Davachi,N. Dadashpour Davachi,N. Dadashpour Davachi,N. Dadashpour Davachi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Bioinformatics,793/B serotype,Infectious bronchitis virus,Molecular features,S1 glycoprotein
- چکیده:
- چکیده انگلیسی: Infectious bronchitis (IB) is an acute, highly contagious, and economically important viral disease of chickens. The S1 subunit from Spike (S) protein plays the major role in protective immunity and is involved in the host-virus interactions, as well as infectious bronchitis virus (IBV) serotyping. Aim of the present study was multi-aspect analysis of the molecular and immunological features of 5' part belonging to the S1 glycoprotein sequence of Iranian 793/B IBV strain isolates. This might ideally help in characterization, prevention, and vaccine development. The tissue samples were prepared, followed by virus isolation, reverse transcription polymerase chain reaction and restriction fragment length polymorphism analysis. In addition, sequencing and registration of the sequences in the National Center for Biotechnology Information were performed. Moreover, 12 sequences were retrieved from Fars province, Iran. The next steps included evaluation of conservation/variability along the sequences, phylogenetic analysis, estimation of the average evolutionary divergence over all the sequence pairs, predicting the phosphorylation/N-glycosylation/palmitoylation sites, and the final analysis of antigenicity. The findings of alignment, entropy plot, and pairwise similarity analysis revealed 17 hypervariable regions. The isolates belonging to Tehran were clustered in phylogenetic tree, and the most similar isolates to them were ADW11182 and ADW11183. Location of some of the N-glycosylation/phosphorylation/palmitoylation points indicated that these sites were conserved among the isolates. Furthermore, the frequency of epitopes and their scores reflect the high immunogenicity of S1 protein in 793/B serotype. Analysis of the primary and secondary structures demonstrated that their parameters had variable values and were different regarding the number and location of α-helix, β-strand, and coils. According to our findings, the Iranian isolates of 793/B serotype change their molecular characteristics during time and in different geographical regions. These alterations might account for failure in prevention programs and differences in virulence and pathogenicity.
- انتشار مقاله: 29-10-1396
- نویسندگان: M. Vasfi Marandi,M. Malekan,M. M. Ranjbar,N. Dadashpour Davachi,S. Alamian
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Electrosurgery,Bovie Machine,Vasectomy,Embryo transfer
- چکیده:
- چکیده انگلیسی: Vasectomy in laboratory animals is a crucial step in the production of surrogate female mice. The surrogate mothers play a key role in successful embryo transfer, most important steps for the production of transgenic animal models, investigation of the preimplantation embryo development, and revitalization of cryopreserved strains. Abdominal and scrotal surgeries are common surgical procedures used in routine veterinary practice to produce vasectomized males. Two different surgical practice, namely electrosurgery and cold surgical practice, have been used as common techniques in operating rooms. Based on current knowledge, there is no published “technical note” as a detailed and step by step guideline to describe vasectomy using an electrosurgery machine (i.e., Bovie machine) in laboratory animal research and breeding facilities.The common problem during the laboratory animal surgery would be animal mortalities as a consequence of profound bleeding. The use of Bovie machine leads to the prevention of profound bleeding during the surgical practice.
- انتشار مقاله: 07-01-1398
- نویسندگان: N. Dadashpour Davachi,N. Dadashpour Davachi,N. Dadashpour Davachi,N. Dadashpour Davachi
- مشاهده