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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Bioengineering Research
- نوع مقاله: Journal Article
- کلمات کلیدی: regeneration,Kidney,Engineering,Scaffold,Decellularized
- چکیده:
- چکیده انگلیسی: Introduction: For regeneration of complex organs, such as the kidney, decellularized (DC) tissues are promising candidates that provide a neutral environment.
Objective: We successfully DC rhesus monkey kidneys by perfusion of trypsin/EDTA, sodium dodecyl sulfate (SDS), triton X-100, and peracetic acid/ethanol. Subsequently, we analysed the samples using quantitative and qualitative analysis.
Methods: Histological staining, DNA quantification and scanning electron microscopy (SEM) was used to investigate cellular removal evaluation. Also, ECM architecture integrity of vascular tree was assessed by x-ray fluoroscopy. The biocompatibility properties of the DC kidney tissues were evaluated by the culture of human embryonic kidney 293 cells (HEK). To evaluate the renal function recovery, 30% of DC tissue was partially grafted into 70% of nephrectomized monkey kidney.
Results: Perfusion-decellularization monkey kidneys retained their essential ECM architecture, intact vascular tree, and cellular compatibility ensured clearance of cellular material, which directly impacts immunoreactivity during transplantation. Quantitative assay and immunohistochemistry demonstrated preservation of native expression patterns and integrity of ECM components, including glycosaminoglycan (GAG), collagen, fibronectin, elastin, and laminin. Also, x-ray fluoroscopy confirmed the integrity and patency of vascular network. Sonography and CT scan images showed significant recovery of renal function after partial transplantation. Histological analysis confirmed increases in renal size and regeneration.
Conclusion: Monkey kidneys could be efficiently decellularized by our novel perfusion-decellularization protocol that can be scaled up for use in human organs. This method represents a step towards development of a transplantable organ using tissue engineering techniques.- انتشار مقاله: 21-08-1398
- نویسندگان: Reza Moghadasali,Soroosh Shekarchian,Mostafa Hajinasrollah,Elham Yousefian,Mostafa Najarasl,Hossein Baharvand,Nasser Aghdami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Amygdala,coping strategies,Stress,Cortisol,Epinephrine,Rhesus macaque
- چکیده:
- چکیده انگلیسی: Objective(s): In the present study the effect of stress on monkeys that had learned to retrieve food from a five-chamber receptacle, as well as the relationship between their behavior and the serum cortisol and epinephrine levels and relative size of the amygdala was evaluated.
Materials and Methods: Six male rhesus monkeys were individually given access to the food reward orderly. They could easily retrieve the rewards from all chambers except for the chamber 4, which a brief, mild electric shock (3 V) was delivered to them upon touching the chamber’s interior. The coping behaviors were video-recorded and analyzed offline. Baseline serum cortisol and epinephrine levels were measured before the experiments using monkey enzyme-linked immunosorbent assay kit. One week after the behavioral experiment, the monkeys’ brains were scanned using magnetic resonance imaging under general anesthesia. The cross-sectional area of the left amygdala in sagittal plane relative to the area of the whole brain in the same slice was evaluated by the planimetric method using ImageJ software.
Results: Exposure to the distressing condition caused different behavioral responses. Monkeys with higher baseline levels of serum cortisol and epinephrine and larger amygdala behaved more violently in the face of stress, indicating adopting emotion-focused stress-coping strategies. Conversely, those with low plasma epinephrine, moderate cortisol, and smaller amygdala showed perseverative behavior, indicating a problem-focused coping style.
Conclusion: In dealing with the same stress, different responses might be observed from nonhuman primates according to their cortisol and epinephrine levels as well as their amygdala dimensions.- انتشار مقاله: 14-05-1396
- نویسندگان: Elaheh Tekieh,Esmail Riahi,Masoomeh Kazemi,Hedayat Sahraei,Hassan Tavakoli,Hamed Aliyary,Mostafa Hajinasrollah,Maryam Salehi,Gholamhossein Meftahi,Mehdi Saberi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Cat,fetus,Ectopic pregnancy,Gestational sac
- چکیده:
- چکیده انگلیسی: Objective: A secondary ectopic pregnancy is defined as continuing development of a fetus in the abdominal cavity after rupture of the uterine wall, which may be associated with a history of trauma. Methods: A cat was referred to the veterinary teaching hospital of the University of Tehran with history of anorexia and vaginal bleeding. In clinical examination some masses werepalpated in abdominal cavity so the patient wasreferred to radiology and sonography for definite diagnoses. In ultrasonography examination three dead fetuses were detectedoutside the uterus. In US it so appeared that gestational sacs did not cover the fetuses and there was a rupture in cranial part of right uterine horn that made the case highly suspected to ectopic pregnancy caused by trauma. Results: Therefore the cat was prepared for aseptic surgery and anesthetized with isofulorane. After incision of the lineaalba three fetuses were seen outside the uterus that wereattached to abdominal wall (left and right wall) and mesentery. There was no sign ofgestational sac to cover the fetuses. The three fetuses were bluntly dissected from the attachments and all conspicuous vessels were ligated and the fetuses were removed. Ovariohysterectomy was also performed to prevent recurrence. Recovery was uneventful.
- انتشار مقاله: 14-06-1394
- نویسندگان: Pedram Mirsepehr,Iman Asheghian,Mostafa Hajinasrollah,Seyed Reza Javadi,Ali Reza Bashiri,Mohamad Reza Esmailinejad,Hamid Taghipour,Seyed Mohamade Mojtahedzade
- مشاهده