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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Streptomyces,Ectoine,Molecular Dynamic,Thermodynamic principles
- چکیده:
- چکیده انگلیسی: In this study, ectoine is produced by Streptomyces. sp IBRC-M PTCC 10615. Fermentation parameters such as flow regime, gas hold up, mass transfer coefficient, and mixing time were optimized by statistical analysis. Streptomyces. sp produced a maximal ectoine concentration of 270 mmol/kg at optimal conditions of ectoine and L-aspartic acid. Also, the amount of mass transfer, gas hold up, and mixing time were determined 0,41/s ,0.3, and 40 s, respectively. The amount of ectoine was measured by HPLC. Furthermore, Molecular Dynamics (MD) simulation was used for studying the solubility of ectoine in aqueous media. Equilibrium data such as temperature, potential energy, and volume graphs showed that the solubility of ectoine is 25%more than glycerol. Also, all the achieving graphs from the equilibrium of simulation were confirmed the appropriate structure of the system.
- انتشار مقاله: 15-02-1398
- نویسندگان: Soheil Rezazadeh Mofradnia,Reihaneh Ashouri,Najmeh Abtahi,Fatemeh Yazdian,Hamid Rashedi,Mojgan Sheikhpour,Fatemeh Ashrafi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Target Therapy,medulloblastoma,Computational Biology,Differ,KEGG pathways
- چکیده:
- چکیده انگلیسی: Introduction: One of the major challenges in cancer treatment is the lack of specific and accurate treatment in
cancer. Data analysis can help to understand the underlying molecular mechanism that leads to better treatment.
Increasing availability and reliability of DNA microarray data leads to increase the use of these data in a variety of
cancers. This study aimed at applying and evaluating microarray data analyzing, identification of important pathways
and gene network for medulloblastoma patients to improve treatment approaches especially target therapy. Methods:
In the current study, Microarray gene expression data (GSE50161) were extracted from Geo datasets and then analyzed
by the affylmGUI package to predict and investigate upregulated and downregulated genes in medulloblastoma. Then,
the important pathways were determined by using software and gene enrichment analyses. Pathways visualization
and network analyses were performed by Cytoscape. Results: A total number of 249 differentially expressed genes
(DEGs) were identified in medulloblastoma compared to normal samples. Cell cycle, p53, and FoxO signaling pathways
were indicated in medulloblastoma, and CDK1, CCNB1, CDK2, and WEE1 were identified as some of the important
genes in the medulloblastoma. Conclusion: Identification of critical and specific pathway in any disease, in our case
medulloblastoma, can lead us to better clinical management and accurate treatment and target therapy.- انتشار مقاله: 17-05-1397
- نویسندگان: Fateme Shaanbanpour Aghamaleki,Behrouz Mollashahi,Nika Aghamohammadi,Nematollah Rostami,Zeinab Mazloumi,Hamidreza Mirzaei,Afshin Moradi,Mojgan Sheikhpour,Abolfazl Movafagh
- مشاهده