در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial activity,GC-MS,Antioxidant property,Rhodococcus rhodochrous,carotenid pigment
- چکیده:
- چکیده انگلیسی: In recent years, scientists and researchers from all over the world pay much attention to carotenoid pigments as a substitute for antibiotics and synthetic chemical compounds with antioxidant property and the plants, various microorganisms including bacteria and algae are also capable of producing carotenoid pigments. In this research, the production of antimicrobial and antioxidant carotenoid pigment in native strain of Rhodococcus bacterium was studied and evaluated for the first time in Iran. The activation and growth of the native Rhodococcus rhodochrous isolate was done using cultivation on different culture media including Triptic soy agar, BHI agar, ISP5, Bennett's Agar and LB Agar at 30 ° C. Pigment extraction was carried out with different organic solvents and carotenoid pigment components were analyzed using GC-MS method. Antimicrobial activity of the pigment was examined using Agar well diffusion method and its antioxidant property by DPPH(2,2-diphenyl-1-Picrilhydrazil) method was examined and analyzed by comparing to antioxidant BHT. The best media for growth and pigment production were determined TSA and TSB. The antimicrobial activity of carotenoid pigment was obtained against to staphylococcus aureus and Candida aibicans with 21mm and 35mm in diameter of inhibition zone respectively. Antioxidant activity of pigment was determined at the concentration of 8 mg/ml (75.59181 %).GC-MS analyses of extracted pigment showed compounds with the highest peak area. The results of this research indicated that native Rhodococcus rhodochrous strain as a non-pathogenic soil bacterium can be candidate biological source with antimicrobial and antioxidant activity for various applications in farmaucidal and biotechnological products.
- انتشار مقاله: 16-01-1396
- نویسندگان: Mahdieh Haddad,Soheil Aghaei,Mohsen Zargar
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Real-time PCR,bla OXA-23,bla OXA-48,E-test,MHT
- چکیده:
- چکیده انگلیسی: Background:
The prevalence of carbapenem-resistant Enterobacteriaceae, especially Klebsiella pneumoniae carbapenemase (KPC), has been recently reported worldwide. Therefore, there is an indispensable need for precise and rapid detection of these carbapenemases.
Objectives:
This study aimed to propose an accurate and rapid method for detecting K. pneumoniae carbapenemase genes from clinical samples, using reverse transcription-polymerase chain reaction (RT-PCR) and to evaluate the expression of these genes in the presence of β-lactam antibiotic by real-time PCR assay.
Methods:
One hundred and eighty-one K. pneumoniae strains were collected from patients presenting to Firoozgar Hospital of Tehran, Iran. The strains were tested using the disk diffusion method, the modified Hodge test (MHT), and E-test minimum inhibitory concentration (MIC). Next, reverse transcription-PCR method was applied for the identification of bla OXA-23 and bla OXA-48 genes. Finally, expression of genes was measured by real-time PCR assay in the presence and absence of β-lactam antibiotic.
Results:
Phenotypic testing also showed a high level of antibiotic resistance, while the genotypic methods indicated the presence and expression of carbapenemase genes.
Conclusions:
The findings suggest revisions in the current antibiotic therapy protocols, considering the high expression level of resistant carbapenemases to K. pneumoniae strains.- انتشار مقاله: 22-03-1398
- نویسندگان: Narjes Mohammadi Bandari,Hossein Keyvani,Mohsen Zargar,Malihe Talebi,Mohammad Reza Zolfaghari
- مشاهده