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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: long non-coding RNA,Polymorphism,Acute Lymphoblastic Leukemia,lnc-LAMC2-1:1,CASC8
- چکیده:
- چکیده انگلیسی:
Long non-coding RNAs (lncRNAs) are a novel class of non-protein coding RNAs that are involved in a wide variety of biological processes. There are limited data regarding the impact of lnc-LAMC2-1:1 rs2147578 as well as CASC8 rs10505477 T>C polymorphisms on cancer development. Here we examined for the first time whether rs2147578 and rs10505477 polymorphisms are associated with childhood acute lymphoblastic leukemia (ALL) in a total of 110 cases and 120 healthy controls. Genotyping was achieved by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The rs2147578 variant increased the risk of ALL in codominant (OR=4.33, 95%CI=2.00-9.37, p<0.0001, CG vs CC, and OR=5.81, 95%CI=2.30-14.69, p=0.0002, GG vs CC), dominant (OR=4.63, 95%CI=2.18-9.86, p<0.0001, CG+GG vs CC), overdominant (OR=1.74, 95%CI=1.02-2.97, p=0.0444, CG vs CC+GG) and allele (OR=1.91, 95%CI=1.32-2.77, p=0.0008, G vs C) inheritance models tested. No significant association was found between the CASC8 rs10505477 T>C variant and risk of childhood ALL. In conclusion, the present study revealed that the lnc-LAMC2-1:1 rs2147578 polymorphism may be a risk factor for developing childhood ALL. Further studies with larger sample sizes with different ethnicities are now required to confirm our findings.- انتشار مقاله: 17-07-1395
- نویسندگان: Mohammad Hashemi,Gholamreza Bahari,Majid Naderi,Simin Sadeghi-Bojd,Mohsen Taheri
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Apoptosis,PD-L1,Polymorphism,PD-1
- چکیده:
- چکیده انگلیسی: Introduction: Programmed cell death-1 (PD-1) and its ligands (PD-L1 and PD-L2) play a critical role as a regulator of immune-system cells, including T cell, natural killer T (NKT), monocytes, dendritic cells (DC), and B cells. Objective: This study aimed to find a possible association between PD-1 (rs11568821, rs2227981, rs2227982), and PD-L1 (rs4143815, rs2890658) variants and Breast Cancer (BC) risk in a sample of southeast Iranian women. Method: The case-control study consisted of 520 individuals, including 260 histologically confirmed BC patients and 260 non-cancer age-matching healthy women as the control group. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Tetra-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction (T-ARMS-PCR) methods were used for genotyping of PD-1 (rs11568821, rs2227981, rs2227982), and PD-L1 (rs4143815, rs2890658) polymorphisms. Results and Conclusion: Our findings indicated that the PD-L1 rs4143815 (G/C) variant meaningfully reduced the risk of BC. However, the PD-L1 rs2890658 variant increased the BC risk in the AC genotype as well as the A allele. Furthermore, we could not find a meaningful association between PD-1 rs11568821, PD-1 rs2227981, PD-1 rs2227982, and BC. Our team examined the possible association between variants and clinicopathological characteristics, including age, size of tumour, lymph node, histology, grade of tumour, estrogen and progesterone receptors status as well as human growth factor receptor 2 (HER2). Our findings demonstrated that PD-L1 rs4143815, PD-L1 rs2890658, PD-1 rs2227982 had a significant association with age. Additionally, we found a significant relation between PD-1 rs2227982 variant and tumour size. Statistical analyzes of PD-1 rs2227981 and PD-1 rs11568821 variants showed a meaningful relation between tumour grade and tumour stage (p=0.006), respectively.
- انتشار مقاله: 12-04-1399
- نویسندگان: Shima Karami,Hedieh Sattarifard,Mohammad Kiumarsi,Sahel Sarabandi,Mohsen Taheri,Mohammad Hashemi,Gholamreza Bahari,Saeid Ghavami
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Multidrug Resistance,LRP,MVP,MRP1
- چکیده:
- چکیده انگلیسی: Background: Breast cancer is the most common malignancy in women. Multidrug resistance (MDR) is still a great
obstacle of breast cancer chemotherapy. We have previously shown that multidrug resistance-associated protein 1 (MRP1)
is associated with response to neoadjuvant chemotherapy. The lung resistance-related protein (LRP) is identified as
a prognostic marker and response to treatment factor which has been studied mainly in hematological malignancy and
leukemia. In this study, we aimed to analyze LRP expression and possible correlation between the expression level of
this gene with MRP1 as a candidate marker for chemotherapy resistance. Materials and Methods: We collected 54
breast tumors and adjacent normal tissues from Iranian breast cancer patients and Real time RT-PCR was employed to
measure the gene expression level in our samples. Results: MRP1 and LRP expression level were significantly lower
in tumor tissues of the patients responding to chemotherapy compared to non-responding patients. No relation between
the expression level of either of these genes and clinicopathology markers was found. Conclusion: Our results suggest
that LRP gene expression is correlated to MRP1 in human breast cancer cells and may affect the clinical response to
treatment.- انتشار مقاله: 13-10-1396
- نویسندگان: Mohsen Taheri,Jamshid Motalebzadeh,Frouzandeh Mahjoubi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Telomere Length,TERT genetic polymorphisms, Childhood acute lymphoblastic leukemia
- چکیده:
- چکیده انگلیسی: Background: Telomeres are involved in chromosomal stability, cellular immortality and tumorigenesis. Human
telomerase reverse transcriptase (TERT) is essential for the maintenance of telomere DNA length. Recently, a variable
tandem-repeats polymorphism, MNS16A, located in the downstream region of the TERT gene, was reported to have
an effect on TERT expression and telomerase activity. Previous studies have linked both relative telomere length
(RTL) and TERT variants with cancer. Therefore, we evaluated associations between RTL, TERT gene polymorphisms
(hTERT, rs2735940 C/T and MNS16A Ins/Del) and risk of childhood acute lymphoblastic leukemia (ALL) in an Iranian
population. Methods: RTL was determined by a multiplex quantitative PCR-based method, and variants of the hTERT,
rs2735940 C/T and MNS16A Ins/Del, were genotyped by amplification refractory mutation system PCR (ARMS-PCR),
and PCR, respectively. Results: Our results indicated that RTL was shorter in ALL patients (1.53±0.12) compared to
the control group (2.04±0.19) (P=0.029). However, no associations between hTERT gene variants or haplotypes and
the risk of childhood ALL were observed (P>0.05). Also hTERT polymorphisms were not associated with RTL or
patient clinicopathological characteristics, including age (P=0.304), sex (P=0.061) organomegally (P=0.212) CSF
involvement (P=0.966) or response to treatment (P=0.58). Conclusions: We found that telomere attrition may be
related to the pathogenesis of childhood ALL, irrespective to TERT variants.- انتشار مقاله: 04-05-1396
- نویسندگان: Ebrahim Eskandari,Mohammad Hashemi,Majid Naderi,Gholamreza Bahari,Vahid Safdari,Mohsen Taheri
- مشاهده