در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Whole-body Irradiation,Nanocurcumin,Herbal radioprotector,Mice survival
- چکیده:
- چکیده انگلیسی: Objective(s): Curcumin, a natural plant product, is commonly known as wonder drug of life, but the poor bioavailability of its free form has hindered its clinical development. The aim of the present study was to investigate the radioprotective effect of nanocurcumin on survival of mice under whole body X-ray irradiation.
Materials and Methods: The Naval Medical Research Institute (NMRI) mice randomly assigned to separate groups and received nanocurcumin via oral gavage at different time points related to irradiation. The survival of mice was evaluated daily for 30 days post-irradiation and finally, the LD50/30 was calculated using Probit analysis. The 30-day survival curve was plotted using the Kaplan-Meier survival curve and the median survival of different subgroups was compared using log-rank test. The P-values less than 0.05 were considered significant.
Results: Our results showed that the administration of oral nanocurcumin could effectively reduce the mortality rate in the irradiated mice. Five days pretreatment with nanocurcumin (4 mg/kg/day) induced maximum radioprotective effect. The LD50/30 was 7.18 Gray (Gy) (95% confidence interval [CI]: 6.59-7.77) and 8.78 Gy (95% CI: 8.14-9.50) for irradiation-only and the optimum nanocurcumin group (pre-irradiation group), respectively (dose reduction factor [DRF] = 1.22). Continued administration of nanocurcumin up to seven days post-irradiation resulted in no further radioprotection.
Conclusions: The results obtained in this study confirmed the efficacy of nanocurcumin as a radioprotective agent against radiation-induced mortality in mice. The specific characteristics of nanocurcumin, such as non-toxicity, edibility, availability, make this phytochemical as a potential radioprotective agent in the radiotherapy setting and radiation accidents. Further clinical studies are highly recommended.- انتشار مقاله: 28-08-1397
- نویسندگان: Reza Sadeghi,Abolfazl Razzaghdoust,Mohsen Bakhshandeh,Farinaz Nasirinezhad,Bahram Mofid
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Whole-body Irradiation,Nanocurcumin,Herbal radioprotector,Mice survival
- چکیده:
- چکیده انگلیسی: Objective(s): Curcumin, a natural plant product, is commonly known as wonder drug of life, but the poor bioavailability of its free form has hindered its clinical development. The aim of the present study was to investigate the radioprotective effect of nanocurcumin on survival of mice under whole body X-ray irradiation.
Materials and Methods: The Naval Medical Research Institute (NMRI) mice randomly assigned to separate groups and received nanocurcumin via oral gavage at different time points related to irradiation. The survival of mice was evaluated daily for 30 days post-irradiation and finally, the LD50/30 was calculated using Probit analysis. The 30-day survival curve was plotted using the Kaplan-Meier survival curve and the median survival of different subgroups was compared using log-rank test. The P-values less than 0.05 were considered significant.
Results: Our results showed that the administration of oral nanocurcumin could effectively reduce the mortality rate in the irradiated mice. Five days pretreatment with nanocurcumin (4 mg/kg/day) induced maximum radioprotective effect. The LD50/30 was 7.18 Gray (Gy) (95% confidence interval [CI]: 6.59-7.77) and 8.78 Gy (95% CI: 8.14-9.50) for irradiation-only and the optimum nanocurcumin group (pre-irradiation group), respectively (dose reduction factor [DRF] = 1.22). Continued administration of nanocurcumin up to seven days post-irradiation resulted in no further radioprotection.
Conclusions: The results obtained in this study confirmed the efficacy of nanocurcumin as a radioprotective agent against radiation-induced mortality in mice. The specific characteristics of nanocurcumin, such as non-toxicity, edibility, availability, make this phytochemical as a potential radioprotective agent in the radiotherapy setting and radiation accidents. Further clinical studies are highly recommended.- انتشار مقاله: 28-08-1397
- نویسندگان: Reza Sadeghi,Abolfazl Razzaghdoust,Mohsen Bakhshandeh,Farinaz Nasirinezhad,Bahram Mofid
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Attenuation Coefficient,Speed of Sound,Radiation Dosimetry,Polymer gel,MAGIC-f
- چکیده:
- چکیده انگلیسی: Background & Aims: Today, different imaging techniques have been studied in the reading of radiationsensitive polymer gels dosage. Due to limitations of imaging techniques such as magnetic resonance imaging (MRI) and computed tomography (CT), ultrasound techniques are proposed for dose-dependent parameter extraction. In this study, using MAGIC-f (methacrylic and ascorbic acid in gelatin initiated by copper with added formaldehyde) polymer gel and megavoltage radiation energy, the dose-dependent parameters of speed of sound and broadband ultrasonic attenuation (BUA) were evaluated. Methods: MAGIC-f polymer gel was irradiated with 1.25 MeV of Cobalt-60 and received a uniform dose in the range of 0 to 60 Gy in 2 Gy steps (2% accuracy). After calibration of ultrasound system with 500 kHz frequency, the parameters of speed of sound and BUA coefficient of exposed polymer gel samples were measured. Based on the dose-response curve, the ability of the ultrasound parameters in reading the absorbed dose was investigated. To evaluate the reasonable time interval with the most sensitive reading of the ultrasound method, samples were studied 3, 24, 36, and 48 hours after irradiation. To study the temperature readings, the gel samples irradiated at 5, 15, and 25 °C were studied. Results: The relationship of variation of speed of sound and attenuation coefficient and quality index (QI) with absorbed dose 3, 24, 36, and 48 hours after irradiation of gels are shown with a fourth-order polynomial fit. The results show that ultrasonic parameters have higher sensitivity 24 hours after irradiation. The sensitivity of dose-speed of sound and attenuation curve were 50 cm/s and 0.06 dB/MHz/Gy based on each Gy in the linear range of 4-44 Gy. Ultrasonic parameters were measured in the gel samples at 5, 15, and 25 ºC after 0-60 Gy irradiation in 2 Gy steps to determine the sensitivity of the ultrasonic properties to temperature. The measurements show that readings at 25 °C have a higher sensitivity than that at 5 and 15 °C. Thus, with an increase of 20 °C, the speed of sound increased 28 and 66 m/s before irradiation and with 60 Gy absorbed dose, respectively. Moreover, the attenuation coefficient decreased 2.50 dB/MHz before irradiation and increased 2.70 dB/MHz with 60 Gy absorbed dose. Conclusion: Acoustic properties of MAGIC-f gel, determined by measurements of speed of sound and ultrasonic broadband attenuation, indicate the ability of this technique in reading of absorbed dose. Maximum sensitivity of reading time and temperature of MAGIC-f gel were 24 hours after irradiation and 25 °C, respectively
- انتشار مقاله: 23-04-1395
- نویسندگان: Hossein Masoumi,Manijeh Mokhtari-Dizaji,Azim Arbabi,Mohsen Bakhshandeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Radiotherapy,Glioblastoma,Temozolomide,Radiation-sensitizing agents
- چکیده:
- چکیده انگلیسی: Background: Glioma is the most common primary brain tumor with poor prognosis. Temozolomide (TMZ) has been used with irradiation (IR) to treat gliomas. The aim of the present study was to evaluate the cytotoxic and radiosensitizing effect of TMZ when combined with high-dose and high-dose rate of gamma irradiation in vitro.Methods: Two ‘U87MG’ cell lines and skin fibroblast were cultured and assigned to five groups for 24, 48, and 72 hours. The groups were namely, TMZ group (2000 μM/L), IR group (5 Gy), TMZ plus IR group, control group, and control solvent group. MTT assay was applied to evaluate cell viability. Data were analyzed with SPSS 21.0 software using one-way ANOVA and Kruskal-Wallis test. P<0.05 were considered statistically significant.Results: The slope of growth curve U87MG cells in semi-logarithmic scale was equal to 27.36±0.89 hours. The viability of cells was determined for different TMZ and IR treatment groups. In terms of cell viability, there were no significant differences between the control and control solvent groups (P=0.35) and between treated group by IR (5 Gy) alone and TMZ (2000 μM/ml) alone (P=0.15). Data obtained for the cell viability of combined TMZ plus IR in both cell lines compared to TMZ or IR treated group alone showed a significant difference (P=0.002). Conclusion: The evaluation of cells viability showed that TMZ in combination with IR on glioma cells led to a significant radiosensitivity compared to IR alone.
- انتشار مقاله: 08-11-1394
- نویسندگان: Samira Borhani,Hossein Mozdarani,Somayyeh Babalui,Mohsen Bakhshandeh,Hassan Nosrati
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Metastasis,head and neck cancer,Keywords: DWI-MRI,neck lymph nodes
- چکیده:
- چکیده انگلیسی: Objective: To evaluate the diagnostic value of DWI in assessment of metastatic neck lymph node in a sample of
Iranian patients with Head and Neck cancer. Methods: 25 patients with 80 neck lymph nodes were analyzed using 1.5 T
MRI. DWI was performed with b values of 0 and 1,000 s/mm2. Short axis diameter and ADC values (min, max and mean)
were calculated for metastatic and non-metastatic neck LNs and results were compared with histopathological findings.
The optimal ADC thresholds were analyzed using receiver coefficient characteristic (ROC) curves for discriminating
between metastatic and benign neck lymph nodes. Result: Histopathological findings revealed that there are 45% (n=36)
metastatic and 55% (n=44) non-metastatic neck LNs respectively. There was no statistically significant difference in
short axis diameter between the two groups (p = 0.346). However, The ADC values of metastatic neck LNs were
significantly lower than those of non-metastatic neck LNs (p < 0.001); 0.90 ± 0.10 × 10-3 mm2/s vs 1.06 ± 0.12 × 10-3
mm2/s ( ADC mean ), 0.78 ± 0.08 × 10-3 mm2/s vs 0.92 ± 0.20× 10-3 mm2/s ( ADC min ) and 1.02 ± 0.12 × 10-3 mm2/s
vs 1.24 ± 0.15 × 10-3 mm2/s (ADC max ). The optimal mean ADC threshold value was equal to 0.996 × 10-3 mm2/s
for differentiating malignant from benign lymph nodes with sensitivity, specificity and accuracy of 80.56 %, 77.27 %
and 71.59 % respectively. Conclusion: MR diffusion imaging and ADC values as a non-invasive technique can assess
metastatic neck LNs in head and neck cancer with higher sensitivity, specificity and accuracy.- انتشار مقاله: 10-09-1397
- نویسندگان: Fatemeh Alamolhoda,Fariborz Faeghi,Mohsen Bakhshandeh,Aslan Ahmadi,Morteza Sanei Taheri,Sahar Aabbasi
- مشاهده