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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Production of extracellular amylase was demonstrated under conditions of high salinity in aerobically cultivated culture of a newly isolated moderately halophilic Gram-positive coccus, designated strain F in basal medium containing peptone from meat, yeast extract, NaCl (7% w/v) and starch. Biochemical and physiological characterization along with 16S rRNA sequence analysis placed F in the genus Nesterenkonia. The enzyme production was synchronized bacterial growth and reached a maximum level during the early-stationary phase in the basal medium. Maximum yield production was observed when the soluble starch, yeast extract and NaCl (1%, 0.75% and 10% w/v, respectively) were used in the fermentation medium with pH 8.0, inoculated with 7% (v/v) pre-culture medium and incubated in 30°C for 72 h with aeration of 250 rpm (299.25 U/ml).
The potential of different carbohydrates in amylase production was in the order: starch> maltose>dextrin>fructose>lactose=glucose>sodium acetate>sodium citrate. The production yield in the presence of different salts was as follows: NaCl> KCl> NaNO3> Na2SO4. Maximum activity for enzyme was assayed in 3.0 M NaCl, pH: 5-7, and 40°C. α-amylase hydrolyzed starch, dextrin and glycogen to form maltose, maltotriose and maltotetraose as major products, and has no effect on amylose and pullulan as substrates. These results suggest that the amylase secreted by Nesterenkonia sp. strain F is industrially important from the perspective of its tolerance to a broad temperature range and its high tolerance to a wide range of salt concentrations (0–3 M NaCl).- انتشار مقاله: 16-07-1391
- نویسندگان: Mohammad Ali Amoozegar,Bardia Samareh-Abolhasani,Mohammad Shafiei,Maryam Didari,Javad Hamedi
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Production of extracellular amylase was demonstrated under conditions of high salinity in aerobically cultivated culture of a newly isolated moderately halophilic Gram-positive coccus, designated strain F in basal medium containing peptone from meat, yeast extract, NaCl (7% w/v) and starch. Biochemical and physiological characterization along with 16S rRNA sequence analysis placed F in the genus Nesterenkonia. The enzyme production was synchronized bacterial growth and reached a maximum level during the early-stationary phase in the basal medium. Maximum yield production was observed when the soluble starch, yeast extract and NaCl (1%, 0.75% and 10% w/v, respectively) were used in the fermentation medium with pH 8.0, inoculated with 7% (v/v) pre-culture medium and incubated in 30°C for 72 h with aeration of 250 rpm (299.25 U/ml).
The potential of different carbohydrates in amylase production was in the order: starch> maltose>dextrin>fructose>lactose=glucose>sodium acetate>sodium citrate. The production yield in the presence of different salts was as follows: NaCl> KCl> NaNO3> Na2SO4. Maximum activity for enzyme was assayed in 3.0 M NaCl, pH: 5-7, and 40°C. α-amylase hydrolyzed starch, dextrin and glycogen to form maltose, maltotriose and maltotetraose as major products, and has no effect on amylose and pullulan as substrates. These results suggest that the amylase secreted by Nesterenkonia sp. strain F is industrially important from the perspective of its tolerance to a broad temperature range and its high tolerance to a wide range of salt concentrations (0–3 M NaCl).- انتشار مقاله: 16-07-1391
- نویسندگان: Mohammad Ali Amoozegar,Bardia Samareh-Abolhasani,Mohammad Shafiei,Maryam Didari,Javad Hamedi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Ornamental Plants
- نوع مقاله: Journal Article
- کلمات کلیدی: Radiation,Gamma ray,Mutagenesis,Chrysanthemum
- چکیده: داوودی یکی از انواع گیاهان زینتی است که به صورت بسیار گستردهای در سطح جهان کشت میشود. این گل به لحاظ تنوع بالای رنگ و شکل دومین گل شاخه بریده و گلدانی در دنیا به شمار میآید. ایجاد موتاسیون روشی مهم برای تولید ارقام جدید است و ارقام بسیاری از طریق جهشهای خودبهخودی و القاء شده تولید شده است. در این پژوهش ریزنمونهی قطعات برگی از سه رقم مهم داوودی با دوزهای مختلف اشعهی گاما مورد پرتوتابی قرار گرفتند. این آزمایش به صورت فاکتوریل در قالب طرح بلوکهای کامل تصادفی در 3 تکرار اجرا شد. بررسی نتایج نشان داد که دوز مناسب پرتو گاما، برای ایجاد جهش در ارقام مورد استفاده در این آزمایش 25 گری است. نتایج بدست آمده نشان داد که دوز 25 گری در رقم بنفش بیشترین تغییر رنگ گلبرگها با نرخ جهش 54/26 درصد را ایجاد کرد. در این بین بیشترین تعداد رنگ جدید متعلق به گروه بنفش کمرنگ بود. همچنین در رقم صورتی خالدار نیز بیشترین تعداد گلهای تغییر رنگ یافته با مقدار تغییر 11/32 درصد در تیمار 25 گری مشاهد شد. بر اساس نتایج بدست آمده در این پژوهش چهار رقم جدید برای ارائه به صنعت گل ایران به عنوان رقم جدید معرفی خواهد شد.
- چکیده انگلیسی: Chrysanthemum is one of the ornamental plants that cultivated widely in worldwide. This flower is one of the most important cut and pot flowers in the world due to its high diversity of colors and shapes. The creation of mutation is an important method for the production of new cultivars, and many cultivars have been produced through spontaneous and induced mutations. In this study, explants of leaf pieces from three most cultivated chrysanthemum varieties were irradiated with different gamma rays. This experiment was conducted as factorial in randomized complete block design with three replications. The results showed that an appropriate dose of gamma ray to produce a mutation in the varieties used in this experiment, is 25 G-ray. The obtained results showed that the 25 G-ray in purple cultivar produced the greatest change in petal color with a mutation rate of 54.56%. Meanwhile, the maximum number of new colors belonged to the purple group. Also, in the pink cultivar, the highest number of colored flowers was observed with a change of 32.11% in the 25 G-ray treatment. Based on the results of this study, four new cultivars will be introduced to the Iranian flower industry as a new cultivar.
- انتشار مقاله: 23-10-1397
- نویسندگان: Mohammad Reza Shafiei,Abdollah Hatamzadeh,Pejman Azadi,Habibollah Samizadeh Lahiji
- مشاهده