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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Gastric acid,Pepsin,Progesterone,Brain injury,Estrogen
- چکیده:
- چکیده انگلیسی: Objective(s) Gastric ulceration is induced by various forms of stress like surgery, ischemia and trauma. The female sex has more resistance to stress and the gastrointestinal lesions happen fewer than male sex. The purpose of this study was to evaluate the role of estradiol and progesterone on the gastric acid and pepsin levels following traumatic brain injury (TBI) induction. Materials and Methods Diffuse TBI was induced by Marmarou method in female rats. Rats randomly assigned into 9 groups: intact, OVX (ovarectomized rat), Sham+OVX, TBI (intact rats under TBI), TBI+OVX (ovarectomized rats under TBI) and treated OVX rats with vehicle (sesame oil), E2 (estradiol), P4 (progesterone) or E2+P4 combination. The acid content and pepsin levels of each gastric washout sample were measured 5 days after the TBI induction. Results There was no significant difference in gastric acid output between groups either after TBI induction or after treatment with E2 or P4 or E2+P4. Gastric pepsin levels were increased in Sham+OVX, TBI (P< 0.001) and TBI+OVX (P< 0.05) compared to intact group. Gastric pepsin levels were significantly lower in E2 and E2+ P4 treated rats than vehicle treated group (P< 0.01). P4 treatment increased gastric pepsin level compared to TBI+OVX group (P< 0.05) and this increment was higher than rats that were treated with the E2 and E2+P4 (P< 0.01). Conclusion These results suggest that protective effect of estradiol and E2+P4 combination against mucosal damage after TBI, might be mediated by inhibition of pepsin secretion.
- انتشار مقاله: 31-06-1394
- نویسندگان: Zakieh Keshavarzi,Mohammad Khaksari Hadad,Mohammad Javad Zahedi,Abbas Bahrami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cell Line,cDNA,NPY,Transfection
- چکیده:
- چکیده انگلیسی: Neuropeptide Y (NPY) is a 36 amino acid peptide found throughout the central and peripheral nervous
system of rat and human. NPY has been proposed to play an important role in satiety. The aim of this study
was to produce cell lines that secrete high levels of bioactive NPY. For this purpose, the complementary
DNA (cDNA) that encodes NPY was isolated by PCR. The cDNA was then cloned into pCEP4, to form
pCEP4NPY. 6-23 cells were transfected with pCEP4NPY by electroporation. Transfected cells were selected by the addition of hygromycin B to the culture medium. Resistant colonies were picked and transferred to 96-well plates. The medium was tested for IRNPY using a specific NPY radioimmunoassay (RIA). The IR-NPY secreted by the cells was characterized by sephadex G50 chromatography and reversed phase fast protein liquid chromatography (FPLC). It was found to co-elute with the synthetic standard in both cases. RNA was extracted from the cells and subjected to Northern blot analysis using labeled NPY cDNA as a probe. The cells were found to express high levels of NPY at mRNA levels.- انتشار مقاله: 12-01-1384
- نویسندگان: Mehdi Mahmoodi,Hamid Reza Rashidinejad,Gholamreza Asadi Karam,Mohammad Khaksari,Ebrahim Mirzajani,Stephen Robert Bloom
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Rats,Colitis,SOD,MDA,GSH,Mumiju
- چکیده:
- چکیده انگلیسی: Objective: In this study, we elucidated the ameliorative effect of aqueous extract of leaves of Mumiju against acetic acid-induced experimental colitis in male rats.
Materials and Methods: The animals were randomly divided into four groups (n=7) including I: control group, II: vehicle group (injected with 2 ml acetic acid (4%) intra rectally), III and IV: treatment groups which received Mumiju (250 mg/kg) orally or intraperitoneally for 4 consecutive days after ulcer induction. Ulcer index, severity of inflammation, colonic levels of superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA), and histological changes were recorded after the treatment regimen of 4 days.
Results: The ulcer index, severity of inflammation and colonic MDA levels were increased following intrarectal instillation of acetic acid. Also, acetic acid significantly decreased the SOD and GSH levels. Treatment with Mumiju for 4 days exhibited significantly lowered oxidative stress, while elevated of SOD and GSH levels. Regenerative-healing patterns also was seen by histopathological findings after treatment with Mumiju.
Conclusion: The present investigation demonstrates that Mumiju could be regarded as a herb with potent therapeutic value in the amelioration of experimental colitis in laboratory animals by modulation of oxidant- antioxidant system.- انتشار مقاله: 12-07-1396
- نویسندگان: Nadar Shahrokhi,Zakieh Keshavarzi,Mohammad Khaksari Haddad,Fereshteh Amirafzali,Shahriar Dabiri,Nava Shahrokhi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: lipid profile,Hypercholesterolemia,Bunium persicum extract,Cardiorespiratory capacity,Endurance Exercise
- چکیده:
- چکیده انگلیسی: Objective(s): We examined the effects of endurance exercise in the presence of Bunium persicum extract administration on lipid profile and cardiorespiratory capacity in hypercholesterolemic male mice.
Materials and Methods: Forty male hypercholesterolemic mice were divided into four groups: Vehicle, Endurance exercise (EE), Bunium persicum extract (BPE), and EE + BPE. The exercise protocol was performed at a speed of 18 m/min, 40 min/day, and 5 days/week for 6 weeks. The BPE was administered orally by a dose of 20 mg/Kg/day.
Results: The results indicated that the 6-week endurance training accompanied by Bunium Persicum extract administration increased cardiorespiratory capacity significantly (601±39 vs. 293±20 meters, p<0.001). Total cholesterol level was significantly reduced in EE + BPE compared with Vehicle and EE groups (p<0.05). LDL-c was lower in EE + BPE compared with the Vehicle (p<0.01). HDL-c in BPE and EE + BPE groups was significantly higher than Vehicle (pConclusion: The results suggested that Bunium persicum extract is very useful in improvement of lipid profile in hypercholesterolemic animals. Supplementation of the extractto exercise significantly increased the cardiorespiratory capacity.- انتشار مقاله: 11-02-1392
- نویسندگان: Mohammad Khaksari,Mohsen Ahmadi,Hamid Najafipour,Nader Shahrokhi
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Methamphetamine,Neurodegeneration,cerebellum,CB1 receptor
- چکیده:
- چکیده انگلیسی: Background: A number of neuroimaging studies on human addicts have revealed that abuse of Methamphetamine (METH) can induce neurodegenerative changes in various brain regions like the cerebral cortex and cerebellum. Although the underlying mechanisms of METH-induced neurotoxicity have been studied, the cellular and molecular mechanisms of METH-induced neurotoxicity remain to be clarified. Previous studies implicated that cannabinoid type 1 receptors (CB1Rs) exert neuroprotective effects on several models of cerebral toxicity, but their role in METH-induced neurotoxicity has been rarely investigated. Moreover, the cerebellum was considered as a potential target to evaluate the effects of cannabinoids on locomotion activity as the CB1Rs are most widely distributed in the molecular layer of cerebellum. Therefore, the present study was carried out to evaluate whether neurodegeneration induced in the cerebellum tissue implicated in locomotion deficit induced by METH.Methods: In the current study, open field test was used to examine locomotor activity. Using hematoxylin and eosin (H&E) staining, morphology of the cerebellar vermis was investigated after repeated exposure to METH. Then, the effects of CB1Rs antagonist [SR17141A, 10 mg/kg, intraperitoneally (IP)] and CB1Rs agonist [WIN55, 212-2 (WIN), 3 mg/kg] against METH-induced neurodegeneration and locomotor deficit were assessed.Findings: The results of the present study demonstrated that repeated exposure to METH increased cerebellar degeneration level as compared to the saline and dimethyl sulfoxide (DMSO) groups. In addition, METH-treated rats showed hyperactivity as compared to the saline and DMSO groups. Pretreatment with WIN significantly attenuated neurodegeneration and hyperactivity induced by METH.Conclusion: The findings of this study provided evidence that CB1Rs may serve as a therapeutic strategy for attenuation of METH-induced locomotor deficits.
- انتشار مقاله: 07-10-1396
- نویسندگان: Effat Ramshini,Shahriar Dabiri,Shokouh Arjmand,Gholamreza Sepehri,Mohammad Khaksari,Meysam Ahmadi,Mohammad Shabani
- مشاهده