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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Nanocomposites,Ibuprofen,poly lactic acid,hydroxyapaptite,release profile,drug deliver
- چکیده:
- چکیده انگلیسی: Gelatin-hydroxyapatite-polylactic acid (PLA) nanocomposites were synthesized using five different formulations. The nanocomposites were loaded with ibuprofen and the amount of drug in the carriers was determined. X-ray diffraction (XRD) analysis was conducted before and after drug loading to ensure the presence of ibuprofen on the nanocomposites. Drug delivery was evaluated in phosphate buffered saline (PBS) solution at pH 7.4 and 37°C. The results of XRD analysis showed acceptable synthesis of hydroxyapatite and the composites, confirming the loading of ibuprofen onto the synthesized nanocarriers. The results showed that maximum drug loading (58.2%) was recorded for sample D (30% gelatin, 40% nHA and 30% PLA), and minimum loading was recorded for sample E (30% gelatin, 30% nHA and 40% PLA). The maximum percentage of drug release over the course of 72 h (95.8%) was for nanocomposite D (30% gelatin, 40% nHA and 30% PLA). The minimum percentage of drug delivery (77%) was for nanocomposite E (30% gelatin, 30% nHA, 40% PLA), which contained the maximum PLA content.
- انتشار مقاله: 15-09-1395
- نویسندگان: Zohreh Nabipour,Mohammad Sadegh Nourbakhsh,Mohammad Baniasadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,Disk diffusion,PCR,mecA protein
- چکیده:
- چکیده انگلیسی: Background and Objective: Methicillin resistance in Staphylococcus aureus is an increasingly important clinical problem. A chromosomal gene, mecA, mediates resistance to penicillinase-resistant penicillins such as methicillin and oxacillin in Staphylococcus aureus. We evaluated the validity of disk diffusion test by using oxacillin, methicillin and cefoxitin disks with consideration of the presence of mecA gene as the reference method for detection of methicillin resistant Staphylococcus aureus (MRSA). Materials and Methods: The susceptibility testing of 222 S. aureus clinical isolates to oxacillin (1 µg), cefoxitin (30 µg) and methicillin (5 µg) was carried out by the disk diffusion method according to the Clinical Laboratory Standards Institute guidelines. Detection of mecA gene was performed using PCR method. Results: An amplified mecA gene of 310 bp was detected in 55% of examined strains by PCR, thus 55% strains were considered MRSA. Sensitivity of oxacillin, methicillin and cefoxitin disks were determined 100%, 99.1% and 98.3% respectively. All MRSA strains in PCR had shown resistance to penicillinase-resistant penicillins by oxacillin disk, but two and one strains were sensitive by cefoxitin and methicillin disk respectively. Thus, oxacillin was the most appropriate disk for detecting MRSA. Conclusion: The prevalence of MRSA in this study is comparable to that found in United States, Canada, Europe and Iran, but the percentage of MRSA isolates is almost twice of percentage reported from Japan.
- انتشار مقاله: 07-07-1393
- نویسندگان: Horieh Saderi,Mehri Habibi,Parviz Owlia,Mohammadreza Asadi Karam
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Neonatology
- نوع مقاله: Journal Article
- کلمات کلیدی: Persian Medicine,Infantile colic,Infant crying
- چکیده:
- چکیده انگلیسی: Background: Infantile colic is a common condition among neonates; however, its etiology is not fully identified. This
study aimed to evaluate this complication and search for treatments by investigating the experiences of Iranian
physicians.
Methods: In this review study, the infantile colic was initially studied in modern medicine. To this end, an electronic
search was performed in databases, such as Web of Science, Medline (via PubMed), Scopus, EBASE, UpToDate
(International databases), Magiran, SID, Irandoc, IranMedex (National databases), and the Google Scholar search
engines. Subsequently, this complication was investigated in the original references of Persian medicine. In this regard,
the words related to the subject matter of the research were taken from the original books of Iranian medicine,
including Al-Mansouri Fi Al-Tibb, Canon, Kholasatol Hekmah, and Kholasat Al-Tajarob.
Results: The results of this study indicated that factors, such as maternal mood during pregnancy, inappropriate
maternal nutrition, gastrointestinal problems, and infantile cerebral problems are some of the causes that have been
mentioned regarding infantile colic in modern and Persian medicine. Other causes, such as spinal nerve stimulation,
insomnia, and sleep apnea are also raised in Persian medicine. Almost all of these causes are due to poor digestion of
milk and pneuma in the gastrointestinal tract. Iranian physicians have considered solutions, such as massage with
special oils, nutrition modification, and the use of hypnotics to reduce pneuma production in the gastrointestinal tract
as well as its side effects. The application of these recommendations together can help better treat infantile colic. In
this regard, the data were categorized by reviewing the works of Iranian scholars, as well as combining modern
medical findings and Iranian experience. Accordingly, a new definition of colic can be provided and some new
treatments are added for infantile colic based on modern and traditional medicine
Conclusion: Since the positive role of some measures, such as massage and swaddling in modern medicine is
confirmed, the investigation and recommendations of Iranian physicians in the field of infantile colic seem to be able to
compensate for the gap in identification, prevention, and treatment of this complication.
- انتشار مقاله: 29-12-1398
- نویسندگان: Mohammad Hosein Asadi,Saeed Changizi-Ashtiyani,Seyed Amir Hossein Latifi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Adjuvant,Fusion protein,Neisseria meningitidis,Outer membrane vesicle,Porin A
- چکیده:
- چکیده انگلیسی: Objective(s): The porins A and B and also outer membrane vesicles (OMVs) of Neisseria meningitidis are used for vaccine purposes. In the present study, we aimed to design a new vaccine candidate based on a fusion of PorA of serogroups A and B of N. meningitidis admixed with OMV and evaluate it in an animal model.
Materials and Methods: After bioinformatic studies, a fusion protein composed of porin A from both serogroups A and B of N. meningitidis was constructed, expressed, and purified by nickel resins. Extraction of OMV of N. meningitidis was performed using a chemical method. The mice were vaccinated subcutaneously in different groups with mixtures of PorA proteins, OMV, and Freund’s adjuvants. Then, the immune responses were measured using the ELISA method. Finally, serum bactericidal activity (SBA) procedure was applied to assay the activity of the immune responses in mice.
Results: Mice received the PorA protein plus Freund’s adjuvant. Mice vaccinated with PorA fusion of serogroups A+B plus Freund’s adjuvant produced more IgG, IgG1, and IgG2a than combinations admixed with OMV. Furthermore, the vaccinated mice tended to direct the IgG responses toward IgG1. Sera of the mice that received PorA+Freund’s and those that received PorA+OMV produced higher bactericidal activity than the controls.
Conclusion: Fusion protein porin A could be a valuable target for developing vaccines against N. meningitidis. Although, Freund’s adjuvant induced the strongest IgG responses, given that Freund’s adjuvant has no human use, and OMV is a human adjuvant, OMV could be considered in vaccine design against N. meningitidis.- انتشار مقاله: 29-02-1398
- نویسندگان: Parviz Afrough,Mohammad Reza Asadi Karam,Farzam Vaziri,Ava Behrouzi,Seyed Davar Siadat
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Adjuvant,Fusion protein,Neisseria meningitidis,Outer membrane vesicle,Porin A
- چکیده:
- چکیده انگلیسی: Objective(s): The porins A and B and also outer membrane vesicles (OMVs) of Neisseria meningitidis are used for vaccine purposes. In the present study, we aimed to design a new vaccine candidate based on a fusion of PorA of serogroups A and B of N. meningitidis admixed with OMV and evaluate it in an animal model.
Materials and Methods: After bioinformatic studies, a fusion protein composed of porin A from both serogroups A and B of N. meningitidis was constructed, expressed, and purified by nickel resins. Extraction of OMV of N. meningitidis was performed using a chemical method. The mice were vaccinated subcutaneously in different groups with mixtures of PorA proteins, OMV, and Freund’s adjuvants. Then, the immune responses were measured using the ELISA method. Finally, serum bactericidal activity (SBA) procedure was applied to assay the activity of the immune responses in mice.
Results: Mice received the PorA protein plus Freund’s adjuvant. Mice vaccinated with PorA fusion of serogroups A+B plus Freund’s adjuvant produced more IgG, IgG1, and IgG2a than combinations admixed with OMV. Furthermore, the vaccinated mice tended to direct the IgG responses toward IgG1. Sera of the mice that received PorA+Freund’s and those that received PorA+OMV produced higher bactericidal activity than the controls.
Conclusion: Fusion protein porin A could be a valuable target for developing vaccines against N. meningitidis. Although, Freund’s adjuvant induced the strongest IgG responses, given that Freund’s adjuvant has no human use, and OMV is a human adjuvant, OMV could be considered in vaccine design against N. meningitidis.- انتشار مقاله: 29-02-1398
- نویسندگان: Parviz Afrough,Mohammad Reza Asadi Karam,Farzam Vaziri,Ava Behrouzi,Seyed Davar Siadat
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Nitric oxide,Nerve Growth Factor,Cryopreservation,Human sperm
- چکیده:
- چکیده انگلیسی: Objective(s):Althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of spermatozoa improved after addition of supplementation of antioxidant molecules to freezing media. Nerve growth factor (NGF) as one of the prosurvival substances has gained great attention in recent years. The aim of this study was the usage of NGF as prosurvival factor after cryopreservation process of human semen samples to assess the motility and viability of sperm, nitric oxide (NO) concentration, and DNA fragmentation in normozoospermic men.
Materials and Methods: Semen samples were collected from 25 normozoospermic men and were divided into fresh semen samples as control group, frozen–thawed semen samples without addition of exogenous NGF, and three groups of semen samples cryopreserved with addition of exogenous NGF (0.5, 1, and 5 ng/ml) in freezing medium. Viability was assessed by eosin-negrosin staining technique. Motility was evaluated with inverted microscope. NO concentration and apoptosis content were measured with flow cytometry.
Results: Results showed that exogenous NGF at 0.5 ng/ml could significantly (P-value <0.05) influence viability, motility, nitric oxide, and DNA fragmentation content.
Conclusion: Exogenous NGF as cryoprotectant improved sperm viability and motility, increased intracellular NO concentration, and decreased apoptosis content in normal human spermatozoa.- انتشار مقاله: 27-12-1393
- نویسندگان: Sara Saeednia,Hosein Bahadoran,Fardin Amidi,Mohammad Hosein Asadi,Mohammad Naji,Parvin Fallahi,Nahid Ataie Nejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Crocin,Neuroprotection,histopathological changes,Ischemia-reperfusion injury
- چکیده:
- چکیده انگلیسی: Objective(s): Some histopathological alterations take place in the ischemic regions following brain ischemia. Recent studies have demonstrated some neuroprotective roles of crocin in different models of experimental cerebral ischemia. Here, we investigated the probable neuroprotective effects of crocin on the brain infarction and histopathological changes after transient model of focal cerebral ischemia.
Materials and Methods: Experiment was performed in four groups of rats (each group; n=8), sham, control ischemia and ischemia treated rats. Transient focal cerebral ischemia was induced by 80 min middle cerebral artery occlusion (MCAO) followed by 24 hr reperfusion. Crocin, at doses 50 and 80 mg/kg, was injected at the beginning of ischemia (IP injection). Neurologic outcome (Neurological Deficit Score, NDS scale), infarct volume (TTC staining) and histological studies were assessed 24 hr after termination of MCAO.
Results: Treatment with crocin, at doses 50 and 80 mg/kg, significantly reduced the cortical infarct volume by 48% and 60%, and also decreased striatal infarct volume by 45% and75%, respectively. Crocin at two different doses significantly improved the NDS of ischemic rats. At histological evaluation, crocin, at dose 80 mg/kg more than 50 mg/kg, decreased the number of eosinophilic (prenecrotic) neurons and reduced the fiber demyelination and axonal damage in ischemic regions.
Conclusion:Our findings indicated that crocin effectively reduces brain ischemia-induced injury and improves neurological outcomes. Crocin also is a potent neuroprotective factor that can be able to prevent histopathological alterations following brain ischemia.- انتشار مقاله: 20-09-1393
- نویسندگان: Javad Raouf Sarshoori,Mohammad Hossien Asadi,Mohammad Taghi Mohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: children,Iran,Persian Medicine,Swaddling
- چکیده:
- چکیده انگلیسی: Background
There are controversial views on the advantages and disadvantages of swaddling. Perhaps an explaination on how to swaddle according to the teachings of traditional Persian Medicine will make it easier to have a better judgment of this kind of treatment.
Materials and Methods
This study was based on the books of famous Persian Traditional Medical scholars as well as modern medical literature, published or in databases.
Results
The study showed that nowadays, the Sudden Infant Death Syndrome (SIDS), and hip joint dislocation were considered as the disadvantages of swaddling while baby's' better sleep was believed to be its advantage. However, in Persian traditional medicine, swaddling was known as a treatment for joint dislocation during labor or a means of preventing the dislocations which might occur while carrying the infant. Based on this aim, a careful method for swaddling was proposed which not only diminished the above mentioned disadvantages but also, provided positive side effects like better sleep and preserved body temperature for the newborn baby.
Conclusion
There is a big difference between the ideas of traditional medical scholars and those of people on swaddling. Therefore, by reviewing the works of Iranian scholars and combining modern medical findings and Iranian experience, we can provide a different definition of swaddling. By teaching it to physicians, nurses and mothers, it can be considered as a treatment in the neonatal complications of childbirth. It is used during childbirth, and taking advantage of other benefits of this practice, also prevents its harm.- انتشار مقاله: 10-02-1397
- نویسندگان: Mohammad Hosein Asadi,Saeed Changizi-Ashtiyani,Amir Hossein Latifi,Mohammad Reza Rajabnejad
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Smart Electrical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Electro Hydraulic Servo System (EHSS),Feedback Error Learning (FEL),Laguerre Controller
- چکیده:
- چکیده انگلیسی: This paper proposes an efficient Tracking method for velocity control of an electro-hydraulic servo system (EHSS) in the presence of flow nonlinearities and internal friction. The tracking method controller is a kind of feedback error learning structure. In the proposed method, the Feedback Error Learning (FEL) algorithm is used to control the velocity. There is no need to compute the system jacobian in FEL method which in turn makes its using more suitable for practical scenarios. This procedure illustrates that EHSS control can be successfully. All derived results are validated by computer simulation of a nonlinear mathematical model of the system.
- انتشار مقاله: 01-02-1394
- نویسندگان: Mohammad Reza Asadi Asad Abad,Amir Reza Zare Bidaki,Mohsen Jahanshahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Interdisciplinary Journal of Virtual Learning in Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Smartization of Schools,Instructional Technology,Strategies and Challenges,SWOT Model,Experts Group
- چکیده:
- چکیده انگلیسی: Context The present era of information and communication technology have affected various aspects of individual and social life. In smart schools, information and communication technologies are accompanied with a comprehensive and holistic model, entering predetermined goals and missions into the field of education. The present study aimed at exploring the challenges and strategies in the use of ICT to smartize schools. Methods A qualitative approach was adopted in the present study in conducting interviews with 3 focus group experts (faculty members, teachers, and specialists in educational technology) in the city of Sanandaj. Semi-structured interview was used for data collection. Criterion-based purposive sampling method was used to study the experts ‘viewpoints (4 faculty members, 7 experienced teachers, and 4 experts of information and communication technology in education). In addition, thematic content analysis was used to encode data and summarize the interviews. Finally, strategies were developed using the environmental analysis of SWOT. Results The findings suggested that smartization of schools have noteworthy strengths. These strengths are as follow: encouraging active learners, developing the identity of students, offering opportunities for interactive learning in educational settings, and promoting qualitative development of education. The weaknesses of school smartization process were insufficiency of specialized human resources, incorrect culture in use of ICT tools, and certain challenges (weakness in policymaking, ICT development strategies, and disruption of structural and infrastructure-related factors. Conclusions Fundamental contributive steps could be taken by minimizing weaknesses and challenges, maximizing strengths and opportunities for smart schools, and adopting appropriate strategies for smart schools by experts.
- انتشار مقاله: 11-10-1348
- نویسندگان: Jamal Salimi,Ghobad Ramezani,Mohammad Asadi
- مشاهده