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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Ki-67 antigen,Oral epithelial dysplasia (OED),Oral squamous cell carcinomas (OSCC),Normal oral epithelium (NOE)
- چکیده:
- چکیده انگلیسی: Introduction: Abnormal cell proliferation appears to be a possible predictor of tumorigenesis, Ki-67 protein
expression is closely related to the cell proliferation and could be used as a biomarker for the growth in the most of
human tumors. The aim of the study: Investigating of Ki-67 expression in the pathological grades of oral epithelial
dysplasia and oral squamous cell carcinomas. Materials and Methods: The sample consisted of 30 formalin-fixed,
paraffin-embedded specimens of oral epithelial dysplasia (OED), 30 other of oral squamous cell carcinomas (OSCC), and
10 normal oral epithelium (NOE) were conventionally stained with hematoxylin and eosin and immunohistochemically
stained with Ki-67 monoclonal antibody. Results: Expression of Ki-67 was restricted to the basal layers in the normal
oral epithelium whereas Ki-67 positive cells in oral epithelial dysplasia (OED) were located in the basal, suprabasal
and spinous layers, Ki-67 expression was increased in high-risk cases. Ki-67 positive cells in well-differentiated
(OSCC) were located mainly in the periphery of the tumor nests, in moderately-differentiated (OSCC) were located
in both peripheral and part of a center of the tumor nests whereas it was diffused in most of the Poorly-differentiated
(OSCC). Statistical analysis indicated a significant difference between the expression in (OED) and (NOE), (OSCC)
and (NOE), and no differences between (OED) and (OSCC). Conclusion: This study has concluded that Ki-67 antigen
could be used as a marker for the histological grading of OED and OSCC, Expression of Ki 67 increased according to
the severity of oral epithelial dysplasia.- انتشار مقاله: 09-04-1397
- نویسندگان: Amer Takkem,Charif Barakat,Safa Zakarea,Khaled Zaid,Johnny Najmeh,Mahdi Ayoub,Mohammad Yaman Seirawan
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Ameloblastoma,Keratocystic odontogenic tumor,Amelogenin,Oncogenesis
- چکیده:
- چکیده انگلیسی: Amelogenin (AMEL), the major structural protein of the enamel organic matrix, constitutes more than 90% of the
enamel’s protein content, Aberrations of amelogenin are thought to be involved in the oncogenesis of odontogenic
epithelium. The expression of amelogenin is possibly an indicator of differentiation of epithelial cells in the odontogenic
tumors. Aim of the study: Investigating the expression of amelogenin in some odontogenic tumors, using an
anti-amelogenin polyclonal antibody, and then compare it with AMEL expression in tooth buds as control. Materials
and Methods: study sample consisted of 10 formalin-fixed, paraffin- embedded specimens of ameloblastoma,
10 Keratocystic odontogenic tumors, and 10 tooth buds were conventionally stained with hematoxylin-eosin and
immunohistochemically with AMEL polyclonal antibody. Results: All of the odontogenic tumors expressed AMEL
in the epithelial component, Intensity of expression in ameloblastoma and Keratocystic odontogenic tumor was lower,
compared with tooth buds, Statistical analysis indicated a significant differences between the tumors and tooth buds.
Conclusion: Amelogenin can be used as a marker for odontogenic epithelium, and the expression of amelogenin is
possibly an indicator of epithelial cells differentiation in the odontogenic tumors, and therefore in prediction of the
histological behavior of odontogenic tumors.- انتشار مقاله: 07-11-1396
- نویسندگان: Safa Zakaraia,Mamdouh Almohareb,Khaled Zaid,Mazen Doumani,Mohammad Yaman Seirawan
- مشاهده