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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Breast cancer,Zinc oxide nanoparticles,Green synthesis,Rubia tinctorum
- چکیده:
- چکیده انگلیسی: Objective(s): Nowadays, nanotechnology has offered great success in resolving concerns in cancer therapy and created a new interdisciplinary field of study incorporating various sciences, such as biology, chemistry and medicine. Apoptosis is a conserved and controlled strategy in regulating cellular growth and proliferation, as well as preserving development and general homeostasis of the body. Zinc oxide nanoparticles (ZnO-NPs) are the most important and widely used nanoparticles. This study aimed to evaluate the apoptosis-inducing properties of the synthesized ZnO-NPs by aqueous extract of Rubia tinctorum against the MCF7 breast cancer cell line.
Materials and Methods: Zinc oxide nanoparticles were synthesized using Rubia tinctorum extract and characterized by some methods including dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM) and x-ray diffraction analysis (XRD). Apoptosis was measured by the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry.
Results: The results of this study showed that the particle size of biosynthesized ZnO-NPs using R.tinctorum extract was about 40 nm and had a spherical morphology. The obtain results of the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry showed that biosynthesized ZnO-NPs effectively and dose-dependently induced apoptosis in the MCF7 breast cancer cells.
Conclusion: Therefore, the biosynthesized ZnO-NPs by watery extract of R. tinctorum can be used in the treatment of many diseases, including cancers.- انتشار مقاله: 27-07-1399
- نویسندگان: Zahra Shamasi,Ali Es-haghi,Mohammad Ehsan Taghavizadeh Yazdi,Mohammad Sadegh Amiri,Masoud Homayouni-Tabrizi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Ethnobotany,Astragalus,Fabaceae,Vascular Plants,World
- چکیده:
- چکیده انگلیسی: Objective: Astragalus L. (Fabaceae) is the largest genus of vascular plants in the world, that comprises an estimated number of 2900 annual and perennial species. The members of this genus have a broad spectrum of usages (e.g. medicine, food, fodder, fuel, ornamental plants, etc.). Here, we present a review of ethnobotanical applications of different species of Astragalus by various ethnic and cultural groupings worldwide, to provide an exhaustive database for future works.
Materials and Methods: Literature survey was performed using Scopus, Google Scholar, PubMed, Medline, and Science Direct, and English and non-English reference books dealing with useful properties of the Astragalus species from 1937 to 2018. Consequently, we reviewed a total of 76 publications that supported lucrative information about various uses of this huge genus.
Results: Several ethnobotanical uses of 90 Astragalus taxa were documented which were mainly originated from Asian and European countries. The two most frequently mentioned Astragalus treatments, were against urinary and respiratory diseases. The most commonly used part was gum and the most frequently used preparation method was decoction.
Conclusion: This review highlights that various Astragalus species have great traditional uses in different ethnobotanical practices throughout the world. However, there is still lack of phytochemical and pharmacological researches on many species of Astragalus and further studies are required to substantiate the therapeutic potential of them which will develop new generation of plant-derived drugs in the near future.- انتشار مقاله: 06-03-1398
- نویسندگان: Mohammad Sadegh Amiri,Mohammad Reza Joharchi,Mohabat Nadaf,Yasamin Nasseh
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Apoptosis,Cytotoxicity,Bryonia aspera
- چکیده:
- چکیده انگلیسی:
Objective: Bryonia aspera (Stev. ex Ledeb) is a plant that grows in northeast of Iran. In the present study, cytotoxic and apoptogenic properties of B. aspera root extract was determined against HN-5(head and neck squamous cell carcinoma) and Hela (cervix adenocarcinoma) cell lines.
Materials and Methods: HN-5 and Hela cell lines were cultured in DMEM medium and incubated with different concentrations of B. aspera root extract. Cell viability was quantitated by MTT assay and the optical absorbance was measured at 570 nm (620 nm as the reference) by an ELISA reader, in each experiment. Apoptotic cells were assessed using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). The B. aspera inhibited 50% growth (IC50) of Hela and HN-5 cell lines at 100±28 μg/ml and 12.5±4 μg/ml, respectively after 48 hr of incubation.
Results: Cell viability assay showed that inhibitory effects of B. aspera were time and dose-dependent in both cell lines, which were consistent with morphological changes, observed under light microscope. Apoptosis was investigated by flow cytometry in which percentage of apoptotic cells increased in a dose and time-dependent manner.
Conclusion: Based on our data, B. aspera has cytotoxic effects in which apoptosis played an important role. Further evaluations are needed to assess the possible anti-tumor properties of this plant.- انتشار مقاله: 20-01-1394
- نویسندگان: Solmaz Pourgonabadi,Mohammad Sadegh Amiri,Seyed Hadi Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Medicinal plants,Ethnobotany,Apiaceae,Non- Medicinal Plants
- چکیده:
- چکیده انگلیسی:
Objective: Apiaceae (Umbelliferae) family is one of the biggest plant families on the earth. Iran has a huge diversity of Apiaceae members . This family possesses a range of compounds that have many biological activities. The members of this family are well known as vegetables, culinary and medicinal plants. Here, we present a review of ethnobotanical uses of Apiaceae plants by the Iranian people in order to provide a comprehensive documentation for future investigations.
Materials and Methods: We checked scientific studies published in books and journals in various electronic databases (Medline, PubMed, Science Direct, Scopus and Google Scholar websites) from 1937 to 2015 and reviewed a total of 52 publications that provided information about different applications of these plant species in human and livestock.
Results: As a result of this review, several ethnobotanical usages of 70 taxa, 17 of which were endemic, have been determined. These plants were used for medicinal and non-medicinal purposes. The most commonly used parts were fruits, leaves, aerial parts and gums. The most common methods of preparation were decoction, infusion and poultice.
Conclusion: To our knowledge, this paper represents a comprehensive literature search of ethnobotanical uses of Apiaceae reported from Iran. This study highlights the rich traditional knowledge of this family that has remained in Iran. However, most of this knowledge survive only as memories from the past in the minds of the elderly, and will probably vanish in a few decades. Thus, we compiled these scattered data together in a single document for the next scientific works with ethnobotanical interests.- انتشار مقاله: 17-09-1394
- نویسندگان: Mohammad Sadegh Amiri,Mohammad Reza Joharchi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Medicinal plants,Mashhad,Ethnobotany,Market
- چکیده:
- چکیده انگلیسی: Objective: An ethnobotanical survey on the medicinal plant species marketed in Mashhad city, northeastern Iran, was conducted in order to document traditional medicinal knowledge and application of medicinal plants.
Materials and Methods: This study was undertaken between 2011 and 2012. The indigenous knowledge of traditional healers used for medicinal purposes were collected through questionnaire and personal interviews during field trips. Ethnobotanical data was arranged alphabetically by family name followed by botanical name, vernacular name, part used, folk use, and recipe. Correct identification was made with the help of the various Floras and different herbal literature at the Ferdowsi University of Mashhad Herbarium (FUMH).
Results: The present investigation reported medicinal information for about 269 species, belonging to 87 vascular plant families and one fungus family. The most important family was Lamiaceae with 26 species, followed by Asteraceae with 23, Fabaceae with 20, and Apiaceae with 19. Herbal medicine uses reported by herbalists was classified into 132 different uses which show significant results to treat a wide spectrum of human ailments. Plants sold at the market were mostly used for digestive system disorders, respiratory problems, urological troubles, nervous system disorders, skin problems, and gynecological ailments.
Conclusion: This survey showed that although people in study area have access to modern medical facilities, a lot of them still continue to depend on medicinal plants for the treatment of healthcare problems. The present paper represents significant ethnobotanical information on medical plants which provides baseline data for future pharmacological and phytochemical studies.- انتشار مقاله: 24-10-1391
- نویسندگان: Mohammad Sadegh Amiri,Mohammad Reza Joharchi
- مشاهده