در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: microscopy,Inflammatory bowel diseases,Blastocystis,demographic data,stool cultivation
- چکیده: زمینه مطالعه: بلاستوسیستیس یکی از شایعترین تکیاختههای بیهوازی موجود در روه بزرگ طیف وسیعی از مهره داران میباشد. هدف: این مطالعه با هدف بررسی شیوع بلاستوسیستیس در بیماران مبتلا به بیماری التهابی روده (IBD) انجام گردید. روشکار: جمع آوری نمونه مدفوع از 80 فرد بیمار مبتلا به IBD انجام شد. همه نمونهها در محیط کشت اختصاصی بلاستوسیستیس کشت داده شدند و جهت تشخیص بلاستوسیستیس تحت بررسی میکروسکپی قرار گرفتند. ارتباط بین اطلاعات دموگرافیک بیماران مبتلا به IBD و تکیاخته بلاستوسیستیس توسط برنامه SPSS نسخه 23 بررسی گردید. نتایج: از 80 بیمار مبتلا به IBD تعداد 52 (65%) مرد و 28 (35%) زن بودند. آلودگی به بلاستوسیستیس در 16 نمونه از 80 نمونه (20%) با روش کشت و توسط میکروسکوپ مثبت گزارش شدند. تکیاخته بلاستوسیستیس در 12 (08/23%) مرد و 4 (29/14%) زن جدا شد. با تست دقیق فیشر هیچ ارتباط معنیداری بین آلودگی به بلاستوسیستیس و جنس نشان داده نشد (0.397 = p value). همچنین هیچ ارتباط معنیداری بین آلودگی به بلاستوسیستیس و ارتباط با حیوان نیز یافت نشد. نتیجهگیری نهایی: در این مطالعه میزان آلودگی به بلاستوسیستیس در افراد مبتلا به IBD 20% گزارش شد. این میزان آلودگی نسبت به سایر گزارشات قبلی که روی میزان شیوع بلاستوسیستیس و این گروه از بیماران انجام شده بود بصورت معنیداری بالاتر گزارش شد.
- چکیده انگلیسی: Background: Blastocystis is the most common anaerobic protozoa living in the large intestine of a broad spectrum of vertebrates. Objectives: The aim of this study was to investigate the Blastocystis infection rate in inflammatory bowel disease (IBD) patients. Methods: A total of 80 stool samples were collected from IBD-proved patients. All stool samples were cultivated in Dulbecco’s modified Eagle’s medium and checked by light microscopy for detection of Blastocystis. The Correlation between demographic data of IBD patients and Blastocystis was calculated using SPSS 23. Results: The enrolled patients comprised of 52 (65%) men and 28 (35%) women. The study showed Blastocystis in 16/80 (20%) of the samples by microscopic examination and culture method. The parasite was seen among 12 (23.08%) and 4 (14.29%) men and women, respectively. No statistically significant correlation was found between infection with the parasite and animal contact. Fisher’s exact test represented that there was no correlation between gender and the presence of Blastocystis (p value= 0.397). Fisher’s exact test denoted that there was no statistical correlation between age and the presence of the parasite (p value= 0.130). Conclusions: In this study, Blastocystis was found in 20% of enrolled patients who suffered from IBD. This infection rate was significantly higher than the studies have previously described Blastocystis in this group of patients.
- انتشار مقاله: 27-09-1396
- نویسندگان: Sara Mohammad Ali Gol,Sedigheh Nabian,Fatemeh Arabkhazaeli,Hamed Mirjalali,Saied Bokaie,Mostafa Rezaeian,Mohammad Reza Zali,Hamid Asadzadeh Aghdaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Colorectal neoplasms,Caco-2 Cells,Macrophages,Monocytes
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is the third most common cancer worldwide. Studies have indicated that immune cells and soluble factors play a key role in maintaining the balance between tumor-promoting inflammation and anti-tumor immunity. It has been shown that secreted cytokines from CRC cell lines could affect peripheral blood mononuclear cells (PBMCs), monocytes, and macrophages phenotypes. Macrophage infiltration has been associated with good prognosis in some cancers, but with poor prognosis in others. The present study aimed to evaluate the effect of conditioned media from CRC cells (Caco-2) on immune responses produced by PBMCs. Methods: The present study was performed at the Gastroenterology and Liver Diseases Research Institute, Shahid Beheshti University of Medical Sciences (Tehran, Iran) in 2017. Human monocytes were isolated from PBMCs by Ficoll gradient media. The co-culture of monocytes and Caco-2 conditioned media was carried out. RNA extraction and cDNA synthesis of monocytes were performed after 96 hours. Gene expression of pro- and anti-inflammatory cytokines was evaluated by real-time PCR. Statistical analysis was performed using the SPSS software (version 21.0) with the independent sample t test. P
- انتشار مقاله: 27-04-1397
- نویسندگان: Bahareh Mohebbi,Kaveh Ashtibaghaei,Mehrdad Hashemi,Mahmoud Hashemi,Hamid Asadzadeh Aghdaei,Mohammad Reza Zali
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: MRSA,Community-Acquired Infections,Vancomycin Resistance
- چکیده:
- چکیده انگلیسی: Objective(s) Staphylococcus aureus is a common cause of human infection, and emergence of vancomycin-resistance S. aureus is a great concern for treatment of methicillin-resistant S. aureus,(MRSA) in recent years (MRSA). Here, we report the isolation of high-level VRSA. Materials and Methods S. aureus was isolated from foot ulcer of a diabetic woman in Tehran, Iran. Antibiotic susceptibility was determined according to CLSI guidelines. VanA gene cluster PCR was carried out and PCR amplicon of vanA was sequenced. Results S. aureus had high-level vancomycin-resistant (MIC 512 ≥ µg/ml). Patient's history revealed that VRSA isolate was acquired through community transmission. Only vanA, vanR and vanS genes were amplified in our isolate. Sequencing revealed that the vanA sequence had high similarity to the vanA sequence of Tn1546. Conclusion Although VRSA infection continues to be rare, isolation of community–acquired VRSA is a significant issue and it needs the efforts of public health authorities.
- انتشار مقاله: 24-06-1394
- نویسندگان: Anahita Dezfulian,Mohammad Mehdi Aslani,Mahvash Oskoui,Parisa Farrokh,Masumeh Azimirad,Hossein Dabiri,1Mohammad Taghi Salehian,1Mohammad Reza Zali
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Heritability,Wheat (Triticum aestivum L.),Gene effects,Androgenesis
- چکیده:
- چکیده انگلیسی: The objective of this study was to estimate genetic parameters and to investigate the type of gene action in controlling androgenesis in wheat. Two wheat cultivars of Grebe and Houtman were reciprocally crossed with two synthetic genotypes of Do1 and Pol and then a complete set of the parents, F1, reciprocal F1 (RF1), F2 and back-cross generations (BC1 and BC2) of each cross were used for anther culture. The ratio of responding anthers, the ratio of albino and green regenerants, and the number of embryoids per each responding anther were determined for different generations of each cross. The results showed a wide genetic variation for embryoid induction and plant regeneration among the parental lines and their progenies. The genetic model of additive-dominance effects could explain the variation among the generation means for the traits, indicating that their inheritance was relatively simple. The genetic analysis also showed predominance of additive genetic effects in genetic control of embryoid induction and green plant regeneration, implying possible improvement of these traits by selection in plant breeding programs. Maternal effects were also found for embryoid induction. The narrow-sense heritability for responding anthers, green plants, albino plants, green plants to total regenerants, and embryoids per responding anther in different crosses varied from 41% to 77%, 64% to 92%, 67% to 84%, 40% to 67% and 33% to 65%, respectively. In conclusion, it seems that the improvement of green plant regeneration in anther culture technique can be achieved by appropriate breeding and selection programs.
- انتشار مقاله: 09-06-1394
- نویسندگان: Badraldin Ebrahim Sayed Tabatabaei,Ghodratollah Saeidi,Mohammad Reza Sabzalian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: transformation,Endophyte,molecular marker,Neotyphodium
- چکیده:
- چکیده انگلیسی: Unique benign endophytes from Ascomycets have wide distribution among grass species. The symbiotic
fungi enhance plant characters including performance, insect and mammalian deterrence, nematode resistance and tolerance to drought, salt and other biotic and abiotic stresses. Endophytes from genus
Neotyphodium (Acremonium) are of the major focus than their ancestors, and Epichloe species, because
the formers have lost their sexual reproduction. Therefore they should be genetically stable, and most
importantly, they cannot disassociate from host tissues, and are transferred vertically. They are maternally
inherited and are therefore attractive for genetic transformation without the concern about gene
escape. Some marker genes have been successfully transferred to endophyte Neotyphodium coenophialum
and Neotyphodium lolii existing in Festuca arundinacea Schreb. and Lolium perenne L., respectively.
Furthermore, gene silencing has been proved to be feasible for eliminating traits, which are economically
harmful. Methods of direct DNA uptake using polyethylene glycol (PEG) and electroporation have been
found to be useful in transformation of these fungi. Transgenic fungi can be reinserted into the host without
need to tissue culture. The endophytic genes responsible for a specific trait can be isolated and transferred to grass species or other microorganisms for direct exploitation of secondary metabolites and endophytic enzymes. Considering advancements in this filed, endophytes can open new horizons faced to scientists and biotechnologist to use them as a surrogate target of transformation.- انتشار مقاله: 13-01-1383
- نویسندگان: Aghafakhr Mirlohi,Mohammad Reza Sabzalian,Mojtaba Khayyam Nekouei
- مشاهده
- جایگاه : پژوهشی
- مجله: Interdisciplinary Journal of Virtual Learning in Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Curriculum,Students,Education,Entrepreneurship
- چکیده:
- چکیده انگلیسی: Background: Entrepreneurship education seeks to provide students with knowledge, skills, attitudes, and motivation to encourage entrepreneurial success in a variety of settings. The purpose of the present study was to perform content analysis of curriculum syllabus for the educational technology discipline based on entrepreneurial competencies. Methods: The research population consisted of all 763 academic curriculums of the educational technology discipline at the University of Tehran during years 2016 to 2017, where the census sampling method was used for their selection. To select them, the purposive sampling method in the entrepreneurs section and census sampling method in the educational technology course syllabus section were used. The research tool in this study was a semi-structured interview and a content analysis checklist was used. This study used a summative qualitative content analysis approach. Initially, interviews were carried out with 12 entrepreneurs to extract entrepreneurial competencies. Then, based on their competencies, a content analysis checklist, including 46 entrepreneurial competencies, was designed in a subset of 6 categories, so that the presence of entrepreneurial competencies in the syllabus of the undergraduate educational technology curriculum was analyzed. To determine the validity of the tool, content validity was used and a check list was provided to three educational specialists and was approved and used after correction. The content analysis construct validity was verified by supervisors, consultants, and entrepreneurs and its reliability was calculated as 94% using William Scott’s method. Results: The results showed that the presence of entrepreneurship competency structure among the curriculum syllabus of educational technology at Tehran University did not have a desirable condition. Conclusions: Therefore, it is imperative that higher education professionals and curriculum developers should take the necessary steps to push students towards entrepreneurship in terms of reviewing the curriculum in educational technology at Tehran University.
- انتشار مقاله: 11-10-1348
- نویسندگان: Ahmad Malekipour,Rezvan Hakimzadeh,Marzieh Dehghani,Mohammad Reza Zali
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal Polyp, mRNA expression,TLR9
- چکیده:
- چکیده انگلیسی: Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in
inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer
cells; however, there is little information about colorectal polyps. Herein, we assessed the mRNA expression of TLR9
in different colorectal polyp types compared to normal group in order to investigate its expression level during CRC
initiation. Fifty-four biopsy samples from colorectal polyp patients and from 20 healthy subjects were collected. The
mucosal mRNA expression level of TLR9 gene was identified by real time PCR. Fold change of gene expression was
evaluated by 2-ΔΔct method. There was a significant relationship between the lower expression of TLR9 gene in the polyp
cases compared to normal individuals (P value = 0.0005), Also, decreased TLR9 mRNA expression was obtained in
adenomas in contrast to hyperplastic and normal groups (P value = 0.0008). Based on the current results, we hypothesized that aberrant surface expression of TLR9 on tumor cells may promote the growth and invasion of colorectal polyps. Further, TLR9 modulation may have an important impact on the development of novel therapeutic strategies.- انتشار مقاله: 12-08-1397
- نویسندگان: Sama Rezasoltani,Shirin Khatibi,Zahra Pezeshkiyan,Ehsan Nazemalhosseini-Mojarad,Maryam Sharafkhah,Amir Sadeghi,Hamid Asadzadeh Aghdaei,Mohammad Reza Zali
- مشاهده