در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Rangeland Science
- نوع مقاله: Journal Article
- کلمات کلیدی: Fire,Grasses,Zagros,Multivariate analysis,Biodiversity indices
- چکیده:
- چکیده انگلیسی: Fire as an ecological factor has both positive and negative effects on components of the ecosystem. The aim of the present study was to investigate the effects of fire on composition, diversity, and functional groups changes in the Tangesorkh rangeland of Boyer-Ahmad County, Iran. The fire took place in the summer of 2015. Two years after the fire, the characteristics of vegetation were measured in the 1 m2 plots. The results showed that 17 species were exclusively found in the fire region, 14 species exclusively in the control region, and 37 species were shared between the two regions. The results of means comparison showed that among the study species, 21 species had a significant response to the fire in terms of canopy cover percent. Fire in the area increased the Simpson and Shannon diversity index and Margalef richness index. The fire caused the canopy cover percent of annuals, perennials, grasses, forbs, Throphytes, Cryptophytes, Hemicryptophytes, Chamaephytes, Asteraceae, Caryophyllaceae, Fabaceae and Rubiaceae and Poaceae increased significantly as compared to control. The redundancy analysis (RDA) results showed that the species had different responses to fire so that the canopy cover of Bromus danthoniae, Vicia villosa, Bromus tomentellus, Astragalus hispanicus, Ziziphora tenuior, Bunium rectangulum, Ferulago angulate and Gundelia tournefortii were increased in the regions where fire occurred. According to the results of this research, functional groups have important roles in determining the responses of plant species to the environmental disturbances; hence, they can affect the secondary succession after the wildfire in rangelands.
- انتشار مقاله: 07-09-1397
- نویسندگان: Parviz Gholami,Mohammad Reza Mirzaei,Ehsan Zandi Esfahan,Alireza Eftekhari
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: AIM2,ASC,Jurkat Cell,MiR-143
- چکیده:
- چکیده انگلیسی: Background: Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play important roles in regulation of immune related genes. However, there is little information regarding the effects of miRNAs on the AIM2 and ASC expression.
Objective: To determine the mRNA levels of AIM2 and ASC in Jurkat cell line following introducing miRNA-143 (MiR-143).
Methods: MiR-143, a scrambled sequence and PBS were introduced separately, to the Jurkat cell lines and the mRNA levels of AIM2 and ASC were examined in parallel with beta-actin and GAPDH (as housekeeping genes) using Real-Time PCR technique.
Results: The mRNA levels of AIM2 and ASC were significantly increased in the MiR-143 transfected Jurkat cells when compared to the scrambled sequence or PBS treated cells.
Conclusions: MiR-143 can lead to increased expression of AIM2 and ASC mRNAs. Considering the significance of AIM2 and ASC in DNA sensing and inflammosome formation, it can be considered as a therapeutic agent for the treatment of chronic infectious diseases, especially viral infections.- انتشار مقاله: 15-05-1395
- نویسندگان: Mohammad Momeni,Mohammad Reza Mirzaei,Nahid Zainodini,Gholamhossein Hassanshahi,Mohammad Kazemi Arababadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: mesenchymal stem cell,Multiple Sclerosis,Immunotherapy,Experimental autoimmune encephalomyelitis,17β- estradiol
- چکیده:
- چکیده انگلیسی: Objective(s): The current investigation was undertaken to evaluate the effects of 17β- estradiol (17β-ED) on the potential of the mesenchymal stem cells (MSCs) for modulation of immunity responses in an animal model of multiple sclerosis (MS).
Materials and Methods: After isolation of MSCs, cells were cultured in presence of 100 nM 17β-ED for 24 hr. Modeling of experimental autoimmune encephalomyelitis (EAE) was achieved by using guinea pig spinal cord homogenate, in addition to complete Freund’s adjuvant in male Wistar rats. The processes of cell therapy were started following 12 days post-immunization. This duration allows all animals to develop a disability score. The achieved EAE clinical symptoms were regularly monitored every day until day 36, when all of examined rats were euthanized.
Results: Cell therapy in the EAE rats with 17β-ED-primed MSCs exhibited more desirable consequences, which in turn lead to regression of the cumulative clinical score and neuropathological changes that are more than the therapy with untreated MSCs. The serum measures of myeloperoxidase (MPO), nitric oxide (NO) as well as splenocytes-originated pro-inflammatory interleukin-17 (IL-17) and tumor necrosis factor alpha (TNF-α) were significantly decreased in EAE rats treated by 17β-ED primed-MSCs compared to EAE rats that received untreated MScs.
Conclusion: Combination of 17β-ED and MSCs more effectively improved the signs and symptoms of EAE.- انتشار مقاله: 07-11-1396
- نویسندگان: Rahim Heidari Barchi Nezhad,Fatemeh Asadi,Mohammad Reza Mirzaei,Seyyed Meysam Abtahi Froushani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: siRNA,HSP40 gene family,OCT4B1,Tumor cell lines
- چکیده:
- چکیده انگلیسی:
Objective(s): The OCT4B1, as one of OCT4 variants, is expressed in cancer cell lines and tissues more than other variants and plays an important role in apoptosis and stress (heat shock protein) pathways. The present study was designed to determine the effects of OCT4B1 silencing on expressional profile of HSP40 gene family expression in three different human tumor cell lines.
Materials and Methods: The OCT4B1 expression was suppressed by specific siRNA transfection in AGS (gastric adenocarcinoma), 5637 (bladder tumor) and U-87MG (brain tumor) cell lines employing Lipofectamine reagent. Real-time PCR array technique was employed for RNA qualification. The fold changes were calculated using RT2 Profiler PCR array data analysis software version 3.5.
Results: Our results indicated that fifteen genes (from 36 studied genes) were down-regulated and two genes (DNAJC11 and DNAJC5B) were up-regulated in all three studied tumor cell lines by approximately more than two folds. The result of other studied genes (19 genes) showed different expressional pattern (up or down-expression) based on tumor cell lines.
Conclusion: According to the findings of the present study, we may suggest that there is a direct correlation between OCT4B1 expression in tumor cell lines (and tissues) and HSP40 family gene expressions to escape from apoptosis and cancer expansion.- انتشار مقاله: 09-12-1394
- نویسندگان: Mohammad Reza Mirzaei,MalekHosein Asadi,Seyed Javad Mowla,Gholamhossin Hassanshahi,Zahra Ahmadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Finance and Managerial Accounting
- نوع مقاله: Journal Article
- کلمات کلیدی: Efficiency,Benchmarking,GANN-DEA,PSOGA,Tobit regression
- چکیده:
- چکیده انگلیسی: The purpose of this study is designing a model based on Tobit regression, DEA, Artificial Neural Network, Genetic Algorithm and Particle Swarm Optimization to evaluate the efficiency and also benchmarking the efficient and inefficient units. This model has three stages, and it uses the data envelopment analysis combined model with neural network, optimized by genetic algorithm, to evaluate the relative efficiency of 16 regional electric companies of Tavanir. A two-staged approach of data envelopment analysis and Tobit regression has been used to measure the effects of environmental variables on the mean efficiency of companies. Finally we use a hybrid model of particle swarm algorithm and genetic algorithm to benchmark the efficient and inefficient units. The mean efficiency of regional electric companies have increased from 0.8934 to 0.9147, during 2012 to 2017, and regional electric companies of Azarbayjan, Isfahan, Tehran, Khorasan, Semnan, Kerman, Gilan and Yazd, had the highest mean efficiency of 1, and west regional electric companies and Fars had the lowest efficiency of 0.7047 and 0.6025, respectively.
- انتشار مقاله: 08-05-1397
- نویسندگان: Mohammad Reza Mirzaei,Mohammad Ali Afshar Kazemi,Abbas Toloie Eshlaghy
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Gastric cancer,Cornus mass L. extract,L929 cells,AGS cell line
- چکیده:
- چکیده انگلیسی: Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the
cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of
apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS
and L929 cells were cultured and treated with different concentrations (0–10 mg/ml) of Cornus mass L. extract for 48
and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified
at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and
double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by
ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation
among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in
comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately
several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC50 value in AGS cells (evaluated
after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry
indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each
of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation
in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.- انتشار مقاله: 17-02-1397
- نویسندگان: Farzaneh Sadat Hosseini,Mojgan Noroozi Karimabad,Mohammad Reza Hajizadeh,Alireza Khoshdel,Soudeh Khanamani Falahati-Pour,Mohammad Reza Mirzaei,Seyed Mehdi Mirmohamadi,Mehdi Mahmoodi
- مشاهده