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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Otorhinolaryngology
- نوع مقاله: Journal Article
- کلمات کلیدی: Consanguinity,Parity,ethnicity,Cleft palate,Cleft lip,Folic acid
- چکیده:
- چکیده انگلیسی: Introduction: A cleft lip with or without a cleft palate is one of the major congenital anomalies observed in newborns. This study explored the risk factors for oral clefts in Gorgan, Northern Iran. Materials and Methods: This hospital-based case-control study was performed in three hospitals in Gorgan, Northern Iran between April 2006 and December 2009. The case group contained 33 newborns with oral clefts and the control group contained 63 healthy newborns. Clinical and demographic factors, including date of birth, gender of the newborns, type of oral cleft, consanguinity of the parents, parental ethnicity, and the mother's parity, age, education and intake of folic acid were recorded for analysis. Results: A significant association was found between parity higher than 2 and the risk of an oral cleft (OR= 3.33, CI 95% [1.20, 9.19], P> 0.02). According to ethnicity, the odds ratio for oral clefts was 0.87 in Turkmens compared with Sistani people (CI 95% [0.25, 2.96]) and 1.11 in native Fars people compared with Sistani people (CI 95% [0.38, 3.20]). A lack of folic acid consumption was associated with an increased risk of oral clefts but this was not statistically significant (OR = 1.42, CI 95% [0.58, 3.49]). There were no significant associations between sex (OR boy/girl = 0.96, CI 95% [0.41, 2.23]), parent familial relations (OR = 1.07, CI 95% [0.43, 2.63]), mother's age and oral clefts. Conclusions: The results of this study indicate that higher parity is significantly associated with an increased risk of an oral cleft, while Fars ethnicity and a low intake of folic acid increased the incidence of oral clefts but not significantly.
- انتشار مقاله: 13-11-1390
- نویسندگان: Mohammad Jafar Golalipour,Nafiseh Kaviany,Mostafa Qorbani,Elham Mobasheri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Neonatology
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Prevalence,ethnicity,Birth defect,Limb
- چکیده:
- چکیده انگلیسی: Background: Congenital limb defects (CLDs) are the leading cause of disability across the world.CLDsvary depending on the anatomical location, type, and cause of anomaly. The present study aimed to evaluate the prevalence and patterns of CLDs in the North of Iran.
Methods: This descriptive-analytical, hospital-based study was conducted in three hospitals in Gorgan, the capital of Golestan Province in the north of Iran. Samples included 32,895 newborns with CLDs, and stillborn neonates were excluded from the study. Data analysis was performed in SPSS version 16 using Chi-square at the significance level of ≤0.05.
Results: Overall prevalence ofCLDs was three cases per 1,000 live births, and the rate was estimated at 3.17 and 2.82 cases per 1,000 live births in male and female infants, respectively. In addition, the prevalence of upper- and lower-limb anomalies was 1.03 and 1.91 cases per 1,000 live births, respectively. In terms ofethnicity, the prevalence of CLDs among the native Fars, Turkmen, and Sistani populations was 3.86, 2.02, and 3.85 cases per 1,000 live births, respectively. Clubfoot was the most common type of CLDs, and the most prevalent type of the associated malformationswas gastrointestinal anomalies.
Conclusion: According to the results, the prevalence of CLDs in the north of Iran was similar to European countries (2.11-3.18 cases per 1,000 live births), while it was lower compared to the other regions in Iran (5.8 cases per 1,000 live births).- انتشار مقاله: 23-12-1396
- نویسندگان: Mohammad Jafar Golalipour,Nafiseh Kaviany,Elaheh Golalipour,Arezoo Mirfazeli,Naser Behnampour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rats,Heart,Gestational diabetes,Hypertrophic genes Offspring
- چکیده:
- چکیده انگلیسی: Objective(s): Gestational diabetes increases the risk of congenital heart disease in the offspring, but the molecular mechanism underlying this process remains unclear. Therefore, the current study was conducted to assess the effects of induced gestational diabetes on expression of some involved genes in cardiac hypertrophy in the offspring of diabetic rats.
Materials and Methods: Diabetes was induced in 40 adult Wistar rats by intraperitoneal injection of 45 mg/kg of streptozotocin. The day of appearance of the vaginal plug was assumed as day zero of gestation for inducing diabetes. After pregnancy, the offspring was maintained until they reach the age of 12 weeks. Then, their hearts were excised and were sectioned for molecular study. We analyzed the expression pattern of some hypertrophic genes by the quantitative real-time RT-PCR.
Results: The mRNA expression levels of all studied genes including c-jun, c-fos, c-myc, alpha-myosin heavy chain (α-MHC), atrial natriuretic factor (ANF) and β-MHC, which are important in cardiomyocyte hypertrophy, were higher in the offspring of the diabetic group compared to controls. Significant differences were found for β-MHC and c-myc with PConclusion: Gestational diabetes upregulates expression of c-jun, c-fos c-myc, α-MHC, ANF and β-MHC genes that are involved in cardiac hypertrophy in the offspring of diabetic rats.- انتشار مقاله: 01-05-1396
- نویسندگان: Elia Saragard Kermani,Zahra Nazari,Mehdi Mehdizadeh,Majid Shahbazi,Mohammad Jafar Golalipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Retina,Gestational diabetes,GFAP,Mellitus,Muller cell
- چکیده:
- چکیده انگلیسی: Objective(s): The Muller cell is the principal glial cell of the vertebrate retina. The expression of Glial fibrillary acidic protein (GFAP) in the Muller cells was used as a cellular marker for retinal damage. This study was done to evaluate the effect of gestational diabetes on retinal Muller cells in rat's offspring.
Materials and Methods: In this experimental study, 12 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally. Dams in control group received an equivalent volume normal saline. Eye of six offspring of each group were removed at postnatal day 28 (P28). The histopathological changes in retina were examined through H&E staining and ultrastructure transmission electron microscopy (TEM). The expression of GFAP was examined using Immunohisto-chemical staining of GFAP in Muller cells. Photographs of retina were taken using Olympus BX51 microscope and a digital camera DP12 and EM LEO906; Zeiss, Germany.
Results: In the control rat's offspring, GFAP expression was not significant in Muller cells. According to the optical microscope images, GFAP expression was observed in the processes of the Muller cell in the inner plexiform layer of retina in offspring of diabetic mothers. In TEM technique, nuclear fragmentation and apoptotic bodies were observed in Muller cell of diabetic offspring.
Conclusion: This study showed that the uncontrolled gestational diabetes can increase GFAP expression in Muller cells and retinal thickness of retinal layer in rat offspring's, therefore uncontrolled gestational can damage the Muller cells.- انتشار مقاله: 12-11-1395
- نویسندگان: Akramsadat Tabasi,Soraya Ghafari,Mehdi Mehdizadeh,Majid Asadi Shekari,Mohammad Jafar Golalipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Gene expression,Gestational Diabetes Mellitus,Langerhans islets,Offspring
- چکیده:
- چکیده انگلیسی: Objective(s): The link between a hyperglycemic intrauterine environment and the development of diabetes later in life has been observed in offspring exposed to gestational diabetes mellitus (GDM), but the underlying mechanisms for this phenomenon are still not clear. Reduced β-cells mass is a determinant in the development of diabetes (type 1 and type 2 diabetes). Some recent studies have provided evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in β-cells proliferation. Therefore, we postulated that GDM exposure impacts the offspring’s β-cells by disruption in the CDK4-pRB-E2F1 pathway.
Materials and Methods: Adult Wistar rats were randomly allocated in control and diabetic group. The experimental group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and control dams at the age of 15 week were randomly scarified and pancreases were harvested. Langerhans islets of diabetic and control groups were digested by collagenase digestion technique. After RNA extraction, we investigated the expressions of the kir 6.2 and CDK4-pRB-E2F1 pathway genes by quantitative real-time PCR.
Results: GDM reduced the expression of CDK4-pRB-E2F1 pathway genes in Langerhans islets cells of offspring. CDK4, pRB and E2F1 pathway genes were downregulated in diabetic islets by 51%, 35% and 84%, respectively. Also, the expression of Kir 6.2 was significantly decreased in diabetic islets by 88%.
Conclusion: We suggest that the effect of gestational diabetes on offspring’s β-cells may be primarily caused by the suppression of CDK4-pRB-E2F1 pathway.- انتشار مقاله: 12-11-1395
- نویسندگان: Zahra Nazari,Mohammad Nabiuni,Mohsen Saeidi,Mohammad Jafar Golalipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Nicotine,Antioxidant,Cell death,Oocytes,TUNEL assay
- چکیده:
- چکیده انگلیسی: Objective(s) Cigarette and nicotine enhances embryogenesis, fertility, pregnancy loss and ultrastructure alterations of oocyte. This study was performed to determine the effect of daily supplementation of vitamin E on oocytes apoptosis in nicotine-treated mice. Materials and Methods In this experimental study, 24 NMARI adult female mice were randomly allocated into four experimental groups. For 30 days, animals in control group (C) were received saline through subcutaneous injection, group I received vitamin E (60 mg/kg/day orally), group II received nicotine (5 mg/kg/day, subcutaneous) and animals of group III received nicotine with vitamin E (60 mg/kg/day orally). After 30 days, the animals were superovulated with PSMG (10 Units) and HCG (10 Units). Next day animals were sacrificed and oocytes were flushed. Collected oocytes were examined through TUNEL assay for the determination of apoptosis through the use of fluorescent microscope. Results The number of retrieved oocytes was 139, 148, 97 and 127 in control, experimental group I, II and III, respectively. Nicotine treatment increased apoptosis in oocytes up to 13.4% whereas oocytes apoptosis was 3.6% in controls. Supplementation with vitamin E in nicotine-treated mice reduced the oocytes apoptosis to 5.5%. Conclusion This study showed that nicotine exposure (5 mg/kg/day for 30 days) can increase apoptosis in oocytes, and supplementation with vitamin E (60 mg/kg/day orally) can reduce the oocytes apoptosis in nicotine-treated mice.
- انتشار مقاله: 25-06-1394
- نویسندگان: Ebrahim Asadi,Mehrdad Jahanshahi,Mohammad Jafar Golalipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rats,Testis,Urtica dioica,seminiferous tubules,Morphometry,Diabet mellitus
- چکیده:
- چکیده انگلیسی: Objective(s) Urtica dioica L. has been known as a medicinal plant in the world. This study was conducted to determine the effects of the hydroalcoholic extract of Urtica dioica leaves on seminiferous tubules of diabetic rats. Materials and Methods Animals were allocated to control, diabetic and protective groups. Treated animals received extract of U. dioica (100 mg/ kg/ day) IP for the first 5 days and STZ injection on the 6th day. After 5 weeks, testes removed and stained with H&E technique. Results Tubular cell disintegration, sertoli and spermatogonia cell vacuolization, and decrease in sperm concentration observed in diabetic in comparison with control and protective groups. External seminiferous tubular diameter and seminiferous epithelial height significantly reduced (P< 0.05) in diabetic compared with controls, and these parameters increased (P< 0.05) in the treated compared with diabetics. Conclusion Hydroalcoholic extract of U. dioica, before induction of diabetes; has protective role on seminiferous tubules alterations.
- انتشار مقاله: 04-07-1394
- نویسندگان: Mohammad Jafar Golalipour,Babak Kabiri Balajadeh,Soraya Ghafari,Ramin Azarhosh,Vahid Khori
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mice,Carbamazepine,Congenital abnormalities,Pyridoxine,Teratology
- چکیده:
- چکیده انگلیسی: Objective(s) Carbamazepine (CBZ) is an antiepileptic drug that is used widely for the treatment of epileptic seizures. Neural tube defects (NTDs), growth retardation, and nail hypoplasia are the most common features of teratogenic effects of this drug. The purpose of this study was to examine the effect of vitamin B6 on the developmental toxicity of CBZ on mice. Materials and Methods Sixty BALB/c pregnant mice were divided into four experimental and two control groups. Two experimental groups received daily intraperitoneal injection (IP) of 30 mg/kg (I) or 60 mg/kg (II) of CBZ on gestational days (GD) 6 to 15. Two other experimental groups received daily IP injection of 30 mg/kg (III) or 60 mg/kg (IV) of CBZ with 10 mg/kg/day vitamin B6 by gavage 10 days prior to gestation and on GD 6 to 15. Two control groups received normal saline or Tween 20. Dams underwent Cesarean section on GD 18 and embryos were harvested. External/macroscopic observation of fetuses was done by stereomicroscope and external examination for malformations was recorded. Data analyzed by ANOVA and X2 test using SPSS software. Results The mean weight and crown-rump of the fetuses in both CBZ-treated experimental groups were significantly reduced compared with those of the control groups. Various malformations were detected such as brachygnathia, eye malformations, NTDs, vertebral deformity, brachydactyly and growth retardation. Vitamin B6 treatment significantly reduced various CBZ-induced malformations. Conclusion This study showed that vitamin B6 has a preventive effect on the developmental toxicity of CBZ in mice that can be pursued further for clinical research.
- انتشار مقاله: 30-06-1394
- نویسندگان: Mohammad Afshar,Seyed Adel Moallem,Javad Baharara,Toktam Takjo,Mohammad Jafar Golalipour
- مشاهده