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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: bovine,Co-culture,FSH,Sertoli,SSCs
- چکیده:
- چکیده انگلیسی: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be increased in vitro, at first. Follicle stimulating hormone (FSH) has been suggested to play a determinant role in the survival of germ cells in addition to increasing spermatogonial proliferation. In this study, the in vitro effects of FSH on spermatogonial cell colony formation were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. The identity of the Sertoli cells and spermatogonial stem cells were confirmed through immunocytochemistry and colony morphology, respectively. Co-cultured Sertoli and spermatogonial cells were treated with FSH in different dose of 10, 20 and 40 IU mL-1 FSH, before colony assay. Results indicated that, FSH increased in vitro colonization of spermatogonial cells in comparison with control group. In conclusion, using FSH provided proper bovine spermatogonial stem cell culture medium for in vitro study of these cells.
- انتشار مقاله: 24-12-1391
- نویسندگان: Reza Narenji Sani,Parviz Tajik,Mohammad Hassan Yousefi,Mansoureh Movahedin,Babak Qasemi-Panahi,Shiva Shafiei,Mahmood Ahmadi Hamedani
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Optoelectronical Nanostructures
- نوع مقاله: Journal Article
- کلمات کلیدی: Optical properties,substrate,Chemical Bath Deposition,Structural Properties,Pbs Thin Film
- چکیده:
- چکیده انگلیسی: Nanocrystalline PbS thin films are deposited on glass and alumina substrates
through the chemical bath deposition technique by creating similar conditions, in order
to investigate the effects of the substrate. The structural and optical properties of PbS
films are investigated by X-ray diffraction, scanning electron microscope, and UV–Vis.
The structural analyses of the films indicate that they are of polycrystalline
configurations and have a face-centered-cubic (fcc) rock-salt structure on different
substrates. The X-ray diffraction shows that the structure peaks occur at slightly higher
angles in the deposited (grown) films on glass substrate than the ones deposited on
alumina substrate. Crystal parameters are exceptionally affected by the type of substrate
as well. SEM images reveal that the surface morphology of the PbS thin films is quite
dependent upon the nature of the substrate. The optical band gaps of the samples are
found to be 1.521 eV for glass and 1.678 eV for alumina substrates, which are higher in
comparison to the bulk value (0.41 eV). According to the obtained results, PbS thin film
on alumina substrate has narrower particle size distribution, better transmission and
lower stress than PbS thin film on glass substrate.- انتشار مقاله: 16-02-1397
- نویسندگان: Sohrab Manouchehri,Javad Zahmatkesh,Mohammad Hassan Yousefi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Activation Energy,Tin(II) oxide nanoparticles,Microwave-assisted hydrothermal technique,Urbach energy
- چکیده:
- چکیده انگلیسی: This paper presents a novel microwave-assisted hydrothermal technique for synthesizing tin(II) oxide nanoparticles. This technique can be used for producing large quantities of homogeneous nanoparticles in a short time. The effect of the solution molarity, final pH, hydrothermal processing time and microwave power were studied. The tin(II) oxide structure verified from XRD and the mean crystallite size was evaluated to be about 5 nm using the Debye-Scherrer formula on the most intense peak. The particle size was measured from STM pictures in the range between 4-5 nm. For different samples, UV-Vis spectroscopy showed the absorption peak due to tin(II) oxide at about 240 nm and an exitonian peak at about 280 nm that shifted with respect to solution molarity, final pH, hydrothermal processing time and microwave power. The photoluminescence spectroscopy (PL) results showed the emission peaks in the visible spectrum range. The results showed that synthesized SnO nanoparticles have a direct bandgap equal to about 2.5 eV, an Urbach energy of about 2.7 eV and activation energy of 47.75kJ/mol.
- انتشار مقاله: 03-03-1396
- نویسندگان: Sohrab Manouchehri,Parviz Boroojerdian,Atefe Marasi,Maryam Amoo,Mohammad Hassan Yousefi
- مشاهده