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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Prenatal diagnosis,Amelogenin gene,Sex determination,X-linked diseases
- چکیده:
- چکیده انگلیسی: Objective(s):To determine the fetal discernment in suspected cases of sex linked recessive disease in the first trimester of pregnancy. Materials and Methods: After collection of 100 Chorionic Villi samples, the DNAs were extracted and baby gender was determined. Meanwhile, after increasing the sensitivity, the system was able to detect the sex of each cell which was obtained by biopsy. Results: Early fetal gender of 100 Chorionic Villi samples were assessed by PCR. After increasing sensitivity of the assay, the sexes in 13 fetuses that were in different cellular stages were detected. Morover, sexes were detected in two unfertilized and one fertilized ovum but without any division. Conclusion: Sex detection of fetus before delivery in the first trimester of pregnancy, will prevent babies with abnormalities being born. It can also be used in detection of recessive sex related diseases in In Vitro Fertilization cases for sex detection and to transfer female fetus to the mother. Our optimized molecular detection system was designed on the basis of amelogenin gene, which can determine the sex in blood, chorionic villi, and single cell in vitro fertilization with high sensitivity and specificity.
- انتشار مقاله: 29-11-1392
- نویسندگان: Amir Abbas Rahimi,Mohammad Hassan Shahhosseiny,Ghasem Ahangari,Jalal Izadi Mobarakeh
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,MIC,Eucalyptus essence,Lavandula essence
- چکیده:
- چکیده انگلیسی: Candida albicans is the most common and important factor causing candidiasis infections in human. As fungi are resistant to anti-fungal agents, and also with respect to the restrictions in treatment of fungal diseases such as their high costs and side effects, study of the combination of herbal medicine is truly required. The aim of the present study was to investigate the effects of herbal essences of eucalyptus and lavandula on the growth of clinical Candida albicans strains which have been separated from mucosal, cutaneous and visceral infections, and compared to those Candida albicans which are resistant and sensitive to caspofungin. The present study has been carried out on 41 Candida albicans taken from patients suffering from mucosal, cutaneous and visceral candidiasis. The degree of samples’ sensitivity to eucalyptus and lavandula were determined through applying broth microdilution method. In the present study, the MIC (minimum inhibitory concentration) of eucalyptus against Candida albicans was determined at 4-1024µl/ml, in which the most growth fluctuations were seen in the densities of 512 and 128 µl/ml. Moreover, the MIC of lavandula was determined at 1-256 µl/ml, and the most fluctuations in its growth were in the density of 32 and 16 µl/ml. Eucalyptus and lavandula essences have a very good anti-fungal effect against Candida albicans. In the present study, lavandula essence proved better anti-candidiasis effects while no considerable difference was seen between the strains sensitive and resistant to caspofungin in its MIC. In higher densities, eucalyptus essence prevents strains from growing. Therefore, it is recommended to investigate the effects of these essences on other pathogenic fungi and bacteria. It is also recommended to evaluate the use of combinations of two or more essences for curing infections or preventing their reoccurrence.
- انتشار مقاله: 01-03-1391
- نویسندگان: Leila Fozouni,Mansour Bayat,Shahla Roudbar Mohammadi,Mohammad Hassan Shahhosseiny
- مشاهده