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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Biomedicine and Public Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Myocardial Infarction,miRNAs,Gene expression,miR-21,Diagnose
- چکیده:
- چکیده انگلیسی: Introduction: Myocardial infarction is the most important cause of death in cardiovascular patients. Recently, biomarkers play a key role in the diagnosis of cardiovascular disease, including myocardial infarction. Several recent studies have suggested that microRNAs (miRNAs), including miR-21, play a pivotal role in acute myocardial infarction (AMI). Therefore, we aimed to investigate the expression levels of miR-21 in patients with AMI.
Methods: A total of 73 patients with AMI and 73 healthy controls who were between 25 and 85 years old were enrolled in this study. Serum levels of total cholesterol, triglycerides, high density lipoprotein-cholesterol, and low-density lipoprotein- cholesterol, cardiac troponin I (cTnI), activities of creatine kinase, creatine kinase-MB, lactate dehydrogenase, aspartate aminotransferase, Prothrombin Time and Partial Thromboplastin Time were measured using commercial kits. The expression levels of miR-21 were measured by quantitative real time –polymerase chain reaction.
Results: Serum levels of cardiac enzymes, including CPK, and CPK-MB, cTnI were significantly higher in the AMI group (P < 0.05), but no significant difference was observed in, LDH, PT, and PTT levels between AMI and control group (P > 0.05). Plasma miR-21 levels in the AMI group was statistically higher than the control group (P < 0.05).
Conclusion: Data suggested that miR-21 can be used as a specific independent or complementary biomarker for AMI diagnosis. The current diagnostic method is proposed for the early diagnosis of heart disease, especially AMI.- انتشار مقاله: 08-10-1397
- نویسندگان: Mohammad Bagher Pirouzpanah,Jila Allafzadeh Dizaji,Farzin Rezazad,Haleh Barmaki,Mina Zarringol,Hosein Bijanpour,Mahboob Soltanali,Mohammad Nouri,Naser Safaie
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Biomedicine and Public Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Myocardial Infarction,miRNAs,Gene expression,miR-21,Diagnose
- چکیده:
- چکیده انگلیسی: Introduction: Myocardial infarction is the most important cause of death in cardiovascular patients. Recently, biomarkers play a key role in the diagnosis of cardiovascular disease, including myocardial infarction. Several recent studies have suggested that microRNAs (miRNAs), including miR-21, play a pivotal role in acute myocardial infarction (AMI). Therefore, we aimed to investigate the expression levels of miR-21 in patients with AMI.
Methods: A total of 73 patients with AMI and 73 healthy controls who were between 25 and 85 years old were enrolled in this study. Serum levels of total cholesterol, triglycerides, high density lipoprotein-cholesterol, and low-density lipoprotein- cholesterol, cardiac troponin I (cTnI), activities of creatine kinase, creatine kinase-MB, lactate dehydrogenase, aspartate aminotransferase, Prothrombin Time and Partial Thromboplastin Time were measured using commercial kits. The expression levels of miR-21 were measured by quantitative real time –polymerase chain reaction.
Results: Serum levels of cardiac enzymes, including CPK, and CPK-MB, cTnI were significantly higher in the AMI group (P < 0.05), but no significant difference was observed in, LDH, PT, and PTT levels between AMI and control group (P > 0.05). Plasma miR-21 levels in the AMI group was statistically higher than the control group (P < 0.05).
Conclusion: Data suggested that miR-21 can be used as a specific independent or complementary biomarker for AMI diagnosis. The current diagnostic method is proposed for the early diagnosis of heart disease, especially AMI.- انتشار مقاله: 08-10-1397
- نویسندگان: Mohammad Bagher Pirouzpanah,Jila Allafzadeh Dizaji,Farzin Rezazad,Haleh Barmaki,Mina Zarringol,Hosein Bijanpour,Mahboob Soltanali,Mohammad Nouri,Naser Safaie
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: MCF-7,Cisplatin,silibinin,Paclitaxel,Combination therapy
- چکیده:
- چکیده انگلیسی:
Herbal-derived medicines have introduced as sources of novel drugs due to minimum systemic side effects. Silibinin as a flavonoid compound has showed with effective chemotherapeutic effects on different cancers. Here, we investigated the impact of combination therapy of silibinin, with paclitaxel and cisplatin in inhibition of proliferation and induction of apoptosis in MCF-7 cells. Cell proliferation was assessed by MTT assay and the percentage of apoptotic cells was measured using flowcytometric assay. Understand of molecular mechanism of this combination related to apoptotic pathway were evaluated by Real Time RT-PCR assays. The IC50 values for silibinin, paclitaxel and cisplatin were 160 ± 22.2 μM, 33.7 ± 4.2 nM and 3.2 ± 0.5 μM, respectively. Paclitaxel and cisplatin induced higher percentage of apoptosis in MCF-7 (P < 0.05). Treatment of cell line with combination of silibinin and paclitaxel or cisplatin showed enhanced early apoptosis 56% and 61%, respectively (P < 0.05). Gene expression patterns demonstrated a significant decrease in anti-apoptotic Bcl-2 with increase in pro-apoptotic Bax, P53, BRCA1 and ATM mRNA levels. Taken together combination therapy of breast cancer cells by applying paclitaxel or cisplatin with silibinin synergistically increases the anti-proliferative effect of single agents.- انتشار مقاله: 08-03-1396
- نویسندگان: Hadi Chavoshi,Vahid Vahedian,Somaiyeh Saghaei,Mohammad Bagher Pirouzpanah,Mortaza Raeisi,Nasser Samadi
- مشاهده