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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Real-time PCR,Hepatocellular carcinoma,Osteopontin,Alpha-fetoprotein
- چکیده:
- چکیده انگلیسی: Background: Hepatocellular carcinoma (HCC) is a high incidence disease in Egypt with a poor prognosis and
survival. Biomarkers are important for diagnosis of HCC at an early stage. Osteopontin (OPN), a glycoprotein secreted by
macrophages, osteoblasts, and T cells, is also highly expressed in a variety of tumors, such as examples in the breast, colon,
and stomach. The present study aimed to correlate the serum level of OPN in HCV-positive hepatocellular carcinoma
patients, with OPN expression in tumor and non-tumor liver tissues in order to identify its efficacy as a biomarker
for diagnosis. Material and Methods: Out of total of 146 patients, 80 were selected for inclusion in the study. Blood
samples as well as specimens of tumor and non-tumor liver tissue were collected. In addition, blood samples from 20
healthy volunteers were obtained as controls. Serum OPN and alpha-fetoprotein (AFP) were evaluated by ELISA for
HCC and control groups. OPN and AFP gene expression were examined by real-time PCR, after homogenization and
DNA extraction from serum samples and liver tissues. Results: It was found that serum OPN levels were significantly
higher in the HCC group compared to normal group (P=0.009), with a strong positive correlation with AFP expression.
However, there was no significant difference between OPN expression in tumor and non-tumor liver tissue. Conclusion:
Serum OPN is highly suggested to be a professional candidate for HCC early diagnosis, with a diagnostic ability and
accuracy equal or higher than for AFP.- انتشار مقاله: 17-08-1396
- نویسندگان: Samah Mamdouh Abdel-Hafiz,Hussam EM Hamdy,Fatma M Khorshed,Tarek S Aboushousha,Gehan Safwat,Mohamed A Saber,Mohamed Seleem,Amira H Soliman
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HCC,TTF1,RAGE,GLUT1,SOX2
- چکیده:
- چکیده انگلیسی:
Objective: To investigate the expression of TTF-1, RAGE, GLUT1 and SOX2 in HCV-associated HCCs and in surrounding non-tumorous liver tissue. Material and Methods: Tissue material from partial hepatectomy cases for HCC along with corresponding serum samples and 30 control serum samples from healthy volunteers were studied. Biopsies were classified into: non-tumor hepatic tissue (36 sections); HCC (33 sections) and liver cell dysplasia (LCD) (15 sections). All cases were positive for HCV. Immunohistochemistry (IHC), gene extraction and quantitative real-time reverse-transcription assays (qRT-PCR) were applied. Results: By IHC, LCD and HCC showed significantly high percentages of positive cases with all markers. SOX2 showed significant increase with higher HCC grades, while RAGE demonstrated an inverse relation and GLUT-1 and TTF-1 lacked any correlation. In nontumorous-HCV tissue, we found significantly high TTF-1, low RAGE and negative SOX2 expression. RAGE, GLUT-1 and SOX2 show non-significant elevation positivity in high grade HCV compared to low grade lesions. TTF-1, RAGE and SOX2 exhibited low expression in cirrhosis compared to fibrosis. Biochemical studies on serum and tissue extracts revealed significant down-regulation of RAGE, GLUT-1 and SOX2 genes, as well as significant up-regulation of the TTF-1 gene in HCC cases compared to controls. All studied genes show significant correlation with HCC grade. In non-tumor tissue, only TTF-1 gene expression had a significant correlation with the fibrosis score. Conclusion: Higher expression of TTF-1, RAGE, GLUT-1 and SOX2 in HCC and dysplasia compared to non-tumor tissues indicates up-regulation of these markers as early events during the development of HCV-associated HCC.- انتشار مقاله: 17-07-1396
- نویسندگان: Tarek Aboushousha,Samah Mamdouh,Hussam Hamdy,Noha Helal,Fatma Khorshed,Gehan Safwat,Mohamed Seleem
- مشاهده