در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Middle East Journal of Cancer
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: We conducted a retrospective analysis to investigate the clinical outcome of combined modality therapy using multiagent chemotherapy, nephrectomy, and radiotherapy in treatment of children with Wilms' tumor.Methods: This study was conducted on 91 cases of newly diagnosed Wilms' tumor from January 2001 until February 2012. Patients were categorized into two groups according to treatment approach: i) preoperative chemotherapy with delayed surgery (group A; n=66) and ii) immediate surgery (group B; n=25).Results: Preoperative chemotherapy showed a 54.5% partial response rate in group A patients. A final stage distribution indicated that the majority of patients (64%) from both groups were considered to be in the early disease stages (I and II). The median follow up was 49 months (range 3-124). The five-year overall survival rate was 66.5%, whereas the event-free survival rate was 62.5%. In univariate analysis, factors associated with statistically significant reduction in overall (P24 months was associated with a significant reduction in the overall survival rate (P=0.038, HR: 0.438, 95% CI: 0.192-0.953), but was not significant in terms of event- free survival (P=0.104, HR: 0.539, 95% CI: 0.256-1.136). Age >24 months (P=0.0095), disease stage (P=0.0014), and blastimal subtype (P=0.006) were associated with significant increases in relapse rate.Conclusion: Preoperative chemotherapy resulted in a final stage redistribution that placed the majority of patients in the early stages of the disease. Age at diagnosis, disease stage, and histological subtype significantly affected survival and relapse rates.
- انتشار مقاله: 06-07-1392
- نویسندگان: Heba A. Sayed,Mona M. Sayed,Mohamed I. El-Sayed
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Pediatric,Argininosuccinate synthase,Hyperammonemia,Ornithine transcarbamylase,Arginase,Urea cycle disorders,Upper Egypt
- چکیده:
- چکیده انگلیسی: Background: The diagnosis of inborn errors of metabolism is generally challenging. We aimed to explore various types of urea cycle disorders (UCDs), and their clinical presentations and biochemical findings among Egyptian pediatric patients.
Materials and Methods: This case-control study was conducted on 86 participants categorized into 43 pediatric patients suspected to have UCDs and 43 age- and sex-matched healthy controls, recruited from the Pediatric Outpatient Clinics, Inpatients Pediatric Departments, PICU and Neonatal Intensive Care Units of Qena, Assiut and Aswan University Hospitals, Egypt. In addition to the clinical assessments and routine laboratory investigations, colorimetric assays of blood lactate and ammonia, and plasma free amino acids assays using high performance liquid chromatography (HPLC), were performed for all included children. For patients with abnormal aminograms, the five enzymes of the urea cycle were measured in their liver tissue homogenates, using chemical methods.
Results: The results showed that 25 out of 43 suspected patients were confirmed to have UCDs. The most frequent type of UCDs was Ornithine transcarbamylase (OTC) deficiency (48%), followed by Argininosuccinate synthase (ASS) deficiency (36%) and the least frequent was arginase (ARG) deficiency (16%). The main clinical presentations were poor oral intake (100%), followed by lethargy (96%), hypotonia (68%), vomiting (64%), and hepatomegaly (48%). There were normal glucose and ABG values with significantly higher ammonia; lactate and the measured plasma free amino acids among patients with UCDs vs. the controls (p˂0.05).
Conclusion: The most frequent types of UCDs among pediatric patients in Upper Egypt were OTC and ASS deficiencies. In addition to clinical suspicion, assays of lactate, ABG, glucose, ammonia and aminogram may be helpful biochemical tests in diagnosing UCDs.- انتشار مقاله: 07-05-1399
- نویسندگان: Tahia H. Saleem,Nagwan I. Rashwan,Mohammed H. Hassan,Bahaa Hawary,Doha Abd-Elraheim Salama,Nahed A. Mohamed,Mohamed Elsayed Mohamed Ahmed,Eman Ahmed Abd-Elmawgood
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: acute myeloid leukemia,Glutathione peroxidase 3 (GPX3), hematologic malignancies, prognosis
- چکیده:
- چکیده انگلیسی: Background: The impact of low expression of Glutathione peroxidase 3 (GPX3) on the clinical course of acute myeloid leukemia (AML) is poorly investigated. Aims: To explore the status of GPX3 expression and analyze its clinical characteristics and prognosis in a cohort of Egyptian patients with AML. Methods: GPX3 mRNA level was assessed by RT-q PCR in 40 newly diagnosed AML patients and 10 healthy controls. Results: The gene expression level was significantly lower in AML patients than the control group (P < 0.001). A cut off value (0.1223) for the discrimination between AML and controls was obtained by ROC curve. According to this cutoff value; the patients were reassigned into 2 groups; 28 patients with lower GPX3 expression and 12 patients with high GPX3 expression. GPX3low expression was significantly associated with higher incidence of induction death (P= 0.037) and lower CR rate (P=0.048). Moreover, GPX3low expression was significantly associated with shorter cumulative 1-year overall survival (OS) (P = 0.001) and disease-free survival (DFS) (P=0.028). Conclusion: GPX3low expression status is considered a poor prognostic factor in AML predicting shorter OS and DFS. The study highlights the importance of targeting glutathione metabolism as a central component of the anti-leukemia therapy.
- انتشار مقاله: 25-04-1399
- نویسندگان: Yasmine Shaaban,Salah Aref,Mona Taalab,Mohamed Ayed,Mohamed Mabed
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: AML,polymorphisms,TLR4,TLR2
- چکیده:
- چکیده انگلیسی: Backgrounds: Toll-like receptors 2; 4 (TLR2;4) are an essential component of the innate immunity and play an important role in immune-surveillance and immune response to various microorganisms. This study aimed to investigate the association between TLR2 and TLR4 polymorphism and the risk of acquiring severe infections, and impact on AML patient’s outcome. Subjects and methods: Using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP); we analyzed three SNPs in the TLR2 (Arg753Gln) and TLR4 (Asp299Gly and Thr399Ile) in 120 AML patients and 100 healthy control subjects. Results: No significant differences in genotype or alleles frequency between healthy controls and AML patients regarding TLR2 Arg753Gln, TLR4 Asp299Gly and TLR4 Thr399Ile polymorphisms (P>0.05 for all). Neutropenic fever was detected in 110 out of 120 (91.7%) of the studied AML patients. The sepsis and pneumonia were identified in 20 out of 120 patients (16.7%). The incidence of sepsis was associated with TLR2 Arg753Gln: AG genotypes, A allele and TLR4 Asp299Gly: CT genotype and C allele as compared to other genotypes and alleles. Moreover; TLR2 (Arg753Gln) GG polymorphisms significantly associated with shortest overall survival (OS) and shortest disease-free survival (DFS); while TLR4 polymorphisms affect the DSF only but not OS. In AML patients TLR2 Arg753Gln gene polymorphism is associated with high susceptibility to sepsis and TLR4 (Asp299Gly and Thr399Ile) gene polymorphism is associated with high susceptibility for both pneumonia; and sepsis. Conclusion: TLR2 Arg753Gln (AG; GG genotype) polymorphisms are associated with shortest OS and DFS. Moreover; significant association between TLR2 polymorphisms, TLR4 Arg753Gln polymorphisms and risk of severe infections in AML patients was documented.
- انتشار مقاله: 27-12-1398
- نویسندگان: Salah Aref,AL Shaimaa Mansoura Abd Elmaksoud,Sherin Abd Elaziz,Mohamed Mabed,Mohamed Ayed
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: AML,NK cells,MDS,sTNF-α,sIL32
- چکیده:
- چکیده انگلیسی: Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disorders that result from the evolution of aberrant clones which lead to leukemia. Disorders of the immune system serve important functions in the pathophysiology and progression of this disorder. This study aimed to assess the bone marrow natural killer cells percentage as well as soluble TNF-α and sIL-32 concentration levels in MDS patients. Methods: Bone marrow samples were obtained from 34 MDS; 12 MDS-AML and 10 controls. The percentage of total NK cells and mature NK cells were determined by flowcytometry. Bone Marrow soluble TNF-α and sIL-32 concentration levels were measured by ELISA. Results: The percentage of total NK and mature NK cells were significantly lower in MDS patients as compared to controls (p
- انتشار مقاله: 27-12-1398
- نویسندگان: Salah Aref,Nada Khaled,Abdel Hady Al Gilany,Mohamed Ayed,Tarek Abouzeid,Doaa Attia
- مشاهده