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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Survival,Breast Neoplasms,carbonic anhydrase IX,Hypoxia-Inducible Factor 1,Tumor Hypoxia
- چکیده:
- چکیده انگلیسی: Objective: Hypoxia-associated biomarkers profiling may provide information for prognosis, staging, and subsequent therapy. We aim to evaluate whether the quantitative gene and protein expression of hypoxic response tumor markers - carbonic anhydrase IX (CAIX) and hypoxia- inducible factor 1 alpha (HIF1A) — may have a role in predicting survival in advanced breast cancer of Indonesian population. Methods: Tumor tissues and peripheral blood samples were collected from treatment - naïve locally advanced (LABC) or metastatic breast cancer patients (MBC) at Wahidin Sudirohusodo General Hospital (Makassar, South Sulawesi) and its referral network hospitals from July 2017 to March 2019. The level of mRNA (of blood and tumor tissue samples) and soluble protein (of blood samples) of CAIX and HIF1A were measured by RT-qPCR and ELISA methods, respectively, besides the standard histopathological grading and molecular subtype assessment. The CAIX and HIF1A expression, patients’ age, tumor characteristics, surgery status, and neoadjuvant chemotherapy drug classes were further involved in survival analyses for overall survival (OS) and progression-free survival (PFS). Results: Forty (30 LABC, 10 MBC) eligible patients examined were 21 hormone-receptors positives (15 Luminal A, 6 Luminal B) and 19 hormone-receptors negatives (10 HER2-enriched, 9 triple-negative). The CAIX blood mRNA and CAIX soluble protein levels in hormone-receptors negative patients were higher than in hormone-receptor-positive patients (p < 0.05). In univariate analysis, both CAIX and HIF1A levels predict OS (except HIF1A protein) with CAIX tissue mRNA has the highest hazard ratio (HR 8.04, 95%CI:2.45-26.39), but not PFS. Cox proportional hazard model confirmed that CAIX tissue mRNA is the independent predictor of OS (HR 6.10, 95%CI: 1.16-32.13) along with surgical status and tumor advancement type (LABC or MBC). Conclusions: CAIX mRNA expression of tumor tissue in treatment-naïve advanced breast cancer has a predictive value for OS.
- انتشار مقاله: 12-05-1398
- نویسندگان: Irwan Gunawan,Mochammad Hatta,Andi Fachruddin Benyamin,Andi Asadul Islam
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: DUSP4,mRNA,qRT-PCR,chemotherapy response
- چکیده:
- چکیده انگلیسی: Objective: Evaluation of the neoadjuvant chemotherapy response can be performed by comparing the breast cancer
burden and pathobiology before and after treatment. This study was aimed to investigate the pattern of dual-specific
phosphatase 4 (DUSP4) mRNA expression in breast cancer cells before and after neoadjuvant chemotherapy. Methods:
This was a longitudinal study. Twenty samples of matched breast cancer tissue taken from biopsy before and after
chemotherapy were subjected to qRT-PCR to detect DUSP4 mRNA expression. Results: The mean value of DUSP4
mRNA expression in prechemotherapy breast cancer patients was 9.906±0.333 and that in breast cancer patients
postchemotherapy was 10.016±1.062. In the responsive group, the rate of DUSP4 mRNA expression increased by
0.476 after chemotherapy. In the nonresponsive group, the proportion of DUSP4 mRNA expression likely decreased
by 1.012. Statistical analysis found no significant correlation between DUSP4 mRNA expression prechemotherapy and
the clinical chemotherapeutic response with p-value = 0.994 (p ≥0.05). A significant correlation was found between the
postchemotherapy DUSP4 mRNA expression and the clinical chemotherapeutic response with p-value = 0.003 (p < 0.5).
Conclusion: No significant difference was found in the mRNA expression of DUSP4 in pre- and post-neoadjuvant
chemotherapy specimens. High DUSP4 expression postchemotherapy shows a substantial correlation with the
chemotherapeutic response.- انتشار مقاله: 08-03-1397
- نویسندگان: Prihantono Prihantono,Andi Nilawati Usman,Christian Binekada,Mochammad Hatta,Andi Asadul Islam
- مشاهده