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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Leukemia,myeloid,Acute,Fms-Like Tyrosine Kinase 3,prognostic significance
- چکیده:
- چکیده انگلیسی: Background: Fms-like tyrosine kinase-3, internal tandem duplication (FLT3-ITD) mutation, is a known predictor for worse outcome in patients with acute myeloblastic leukemia (AML). However, the prognostic significance of FLT3-ITD mutation in adult, non-transplant patients is still unclear therefore we conducted a systematic review and meta-analysis to explain this issue. The main outcome was overall survival (OS), while additional outcomes included event-free survival (EFS). Methods: Seven Databases (ScienceDirect, Scopus, PubMed, Cochrane, SpringerLink, ProQuest, and EBSCOhost) were searched up to August 2020. Studies investigating the prognostic value of AML in adults with FLT3-ITD mutational status were selected. Studies which patients had received transplantation, diagnosed with acute promyelocytic leukemia (APL) or secondary AML were excluded. The selected studies were divided into subgroups based on their cytogenetic profile. Summary hazard ratios (HR) and 95% confidence intervals (CI) were calculated using fixed-effects models. Heterogeneity tests were conducted and presented in I2 value. Forest plot was presented to facilitate understanding of the results. Publication bias was analyzed by Funnel Plot test. Results: A total of ten studies describing research conducted from 1999 to 2020, met the inclusion criteria for this study. Nine studies reported OS and four studies reported EFS in HR. The highest HR for OS is 6.33 (95% CI, 2.61-15.33; p < 0.001), for EFS is 3.58 (95% CI, 1.59 – 8.05); p = 0.002)., while the lowest for OS is 1.33 (95% CI, 0.88-2.01; P = 0.174) and for EFS is 1.29 (95% CI, 0.75-2.23; p = 0.34). Nine studies were included in meta-analysis with HR for OS 1.91 (95% CI, 1.59–2.30, p < 0.00001), whereas 4 studies were included in meta-analysis for EFS with HR 1.64 (95% CI, 1.25–2.14; p = 0.0003). Conclusion: FLT3-ITD mutation is associated with worse prognosis in adult, non-transplant patients with AML, both for OS and EFS.
- انتشار مقاله: 18-08-1398
- نویسندگان: Ikhwan Rinaldi,Melva Louisa,Fikri Ichsan Wiguna,Elizabeth Budiani,Jeffrey Christian Mahardhika,Khairul Hukmi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: imatinib,ABCB1,Chronic myeloid leukemia,C1236T
- چکیده:
- چکیده انگلیسی: Objective: Imatinib is the first-line drug used for the treatment of chronic myeloid leukemia (CML) patients due to high molecular response and overall survival rate. However, some patients develop resistance to imatinib even after attaining a response. Mutation in ABCB1 efflux transporters is one of the known mechanisms of resistance to imatinib in chronic myeloid leukemia patients. This study was aimed to investigate the association of ABCB1 C1236T polymorphism in Indonesian chronic myeloid patients with molecular response to imatinib treatment. Methods: We analyzed 120 samples from chronic myeloid leukemia patients in the chronic phase, who had been on imatinib treatment for at least 12 months. We analyzed the C1236T variant of the ABCB1 gene using PCR, followed by direct sequencing, and associate them with the achievement of major molecular response (MMR). Results: The major molecular response was achieved in 28% of patients. The frequencies of the SNPs were CC (40%), CT (46%), and TT (14%). Our result showed that there was a lack of association between polymorphism of ABCB1 C1236T and imatinib response in Indonesian patients, with OR = 0.646 (95% CI: 0.283, 1.471; p>0.05). Conclusion: There was no association between ABCB1 C1236T variants with the major molecular response in Indonesian chronic myeloid leukemia patients receiving imatinib treatment.
- انتشار مقاله: 09-09-1397
- نویسندگان: Ikhwan Rinaldi,Riki Nova,Reni Widyastuti,Rizky Priambodo,Instiaty Instiaty,Melva Louisa
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Curcumin,endoxifen,epithelial-mesenchymal transition (EMT),Vimentin,TGF-β1
- چکیده:
- چکیده انگلیسی: Background: Curcumin was shown to reduce epithelial-mesenchymal transition (EMT) markers in previous short
term studies. This study was aimed to investigate the potential of curcumin in the prevention of EMT activation in
MCF-7 cells induced by endoxifen. Methods: MCF-7 breast cancer cells were treated with Endoxifen 1000 nM+betaestradiol
1 nM with or without curcumin (8.5μM or 17 μM). Cells treated with dimethyl sulfoxide (DMSO) 0.001%
were used as negative control. After 8 weeks of continuous treatment, the cells were counted, analyzed for mRNA
E-cadherin, vimentin, TGF-β expression, total reactive oxygen species (ROS) and observed for morphological changes
using confocal microscope and transmission electron microscope. Result: MCF-7 cell viability was increased in
endoxifen + β-estradiol group. Cell viability was significantly decreased in curcumin 17 μM, but not in curcumin
8.5 μM group. Analysis of EMT markers at week 8 indicates that there were increase in vimentin and TGF-β mRNA
expressions, while E-cadherin mRNA expressions and TGF-β1 protein concentrations were shown to decrease. The
results showed that administration of curcumin in all the dose administered were incapable improving the expressions
of vimentin, TGF-β1 and E-cadherin. There was a decrease in ROS concentration in curcumin treated cells (8.5 μM)
while in curcumin 17 μM, ROS concentration was increased. Morphological observation using confocal microscope
and TEM showed the presence of mesenchymal cells and adherens junction. Conclusion: endoxifen treatments for
eight weeks resulted in upregulation of EMT markers and changes in morphology of MCF-7 breast cancer cells. The
addition of curcumin did not prevent the activation of EMT.- انتشار مقاله: 20-04-1396
- نویسندگان: P Paramita,Bantari WK Wardhani,Septelia Inawati Wanandi,Melva Louisa
- مشاهده