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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: anxiety,depression,NMDA receptor,Psilocybe cubensis,psilocybin
- چکیده:
- چکیده انگلیسی: Background and objectives: Considering the increasing prevalence of depression, many studies are launched to investigate new antidepressant treatments. The present research has shown how psilocybin as an active compound of Psilocybe cubensis (Earle) Singer extract (PCE) can change the parameters related to depression and anxiety in animal models. Both serotonin (5-hydroxytryptamine: 5-HT) and glutamate modulate depressive-like behaviors and, therefore, we examined the possible interaction of psilocybin as 5-HT1 agonist with glutamate receptor N-methyl-D-aspartate (NMDA). Methods: Psilocybe cubensis extract of this mushroom was prepared by ethyl acetate. NMRI mice involved in all experiments and were treated with the vehicle, extract, or standard drug intraperitoneally. Open field (OFT), forced swimming (FST) and tail suspension tests (TST) were applied to measure the intended parameters. OFT was performed to verify the applied doses for measuring the following antidepressant activity. Results: PCE at the doses of 100 mg/kg significantly changed the locomotion, time spent in center and velocity of the animals in OFT. While treatment of the animals with PCE 10 and 40 mg/kg or ketamine 1 mg/kg did not alter the locomotor activity, co-administration of these subeffective amounts significantly reduced the immobility time in both FST and TST. Conclusion: These effects may indicate possible implication of psilocybin with NMDA receptor which consequently produces the antidepressant effects.
- انتشار مقاله: 01-07-1396
- نویسندگان: Elaheh Mahmoudi,Mehrdad Faizi,Reza Hajiaghaee,Ali Razmi*
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mitochondria,MTBE,morphology,embryotoxicity,Placenta,Mouse fetus
- چکیده:
- چکیده انگلیسی: Although the biokinetics, metabolism, and chemical toxicity of methyl tert-butyl ether are well known, little attention was paid to the potential toxic effects of MTBE on reproduction and development in mammals. To evaluate the effects of MTBE on pregnant animals, two groups (control and test) of NMRI mice were chosen. In test group 500 and 1000 mg/Kg of it were administered intraperitonealy at 11 days of gestation and in control group no injection was made. Caesarean section was performed at 15 days of the gestation, and the fetus and placentas were examined externally. Based on our morphological results, MTBE caused significant increase (p < 0.05) in the weight of fetuses and the weight of placentas, the diameter of placentas and crown-rump length of fetuses. Also, our mitochondrial results showed significant (p < 0.05) increase in mitochondrial swelling, ROS formation and also significant (p < 0.05) decreased in MMP on mitochondria isolated from liver and brain in test group. These results suggest that MTBE through ROS formation may induce the mitochondrial dysfunction which in turn leads to inhibition of angiogenesis and morphological alterations in fetus of mouse.
- انتشار مقاله: 29-03-1397
- نویسندگان: Mehrdad Faizi,Fatemeh Jamal,Baharak Mohammadzadeh Asl,Parvaneh Naserzadeh,Zeinab Saadabadi,Ahmad Salimi,Jalal Pourahmad Jaktaji
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Mouse fetus,Developmental toxicity,PFOS,Neurobehavioral toxicity,Isolated mitochondria,Brain anomaly
- چکیده:
- چکیده انگلیسی: Perfluorooctanesulfonate (PFOS) is a widely spread environmental contaminant. It accumulates in the brain and has potential neurotoxin effects. Due to chemical properties, PFOS shows persistency in the environment and therefore has potential hazardous effect. The risk of possible intra uterine exposure to PFOS poses a health concern for developmental effects. The goal of this study was survey of histological and behavioral changes made by PFOS in pregnant mice and their fetuses using common behavioral assays and H&E staining. In the present study doses of PFOS (1,10,20 mg/kg) given orally to pregnant mouse from gestational day (GD) 0 to GD14; then on the day 15, Behavioral experiments including (open field and passive avoidance) were used to assess toxic behavioral changes such as memory impairment and anxiety. After behavioral evaluations fetuses were dissected on day 15 of gestation and morphological and histological studies on pregnant mouse brain and her fetus were carried out using haematoxylin-eosin staining method. Our findings showed that PFOS can induce neurotoxicity in pregnant mouse especially by induction of abnormalities in dentate gyrus of hippocamps and disruption of neurobehavioral functions .Besides in her fetus; PFOS produced significant changes in brain, liver and thyroid gland in comparison with untreated control mouse fetus. As a conclusion, PFOS can cause both neurobehavioral and developmental toxicity in pregnant mouse and her fetus.
- انتشار مقاله: 10-09-1394
- نویسندگان: Freshteh Mehri,Mehrdad Faizi,Farzad Kahrizi,Baharak Mohammadzadeh Asl,Jalal Pourahmad Jaktaji
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Mitochondria,Apoptosis,Melanoma,Turbo coronatus,Gel filtration
- چکیده:
- چکیده انگلیسی: Objective: A variety of approaches such as surgery, chemotherapy, radiotherapy, hormonal therapy and
immunotherapy are used to treat melanomas, but unfortunately in most case, the response is very weak and often side
effects are serious. This study concerns selective toxicity of an extract of Turbo coronatus on cells and mitochondria from
a syngeneic mouse model of melanoma. Methods: Cells and mitochondria isolated from extra tumoral and melanoma
tissues were exposed toa T. coronatus crude extract and fractions obtained by gel-filtration chromatography and assayed
for mitochondrial and cellular parameters. Result: Crude extract (375, 750 and 1,500 μg/ml) and fraction 1; F1; (275,
550 and 1100 μg/ml) of T. coronatus extract induced a significant (p<0.05) increase of the reactive oxygen species
(ROS) level, swelling of mitochondria, collapse of mitochondrial membrane potential (MMP), release of cytochrome
c and caspase-3 activation only in the mitochondria and cells obtained from melanoma but not extra tumoral tissues. In
addition, the F1 fraction decreased the percentage of viable cells and induced apoptosis in melanoma cells. Conclusion:
For the first time we could demonstrate that the F1 fraction of a T. coronatus extract, selectively induces ROS mediated
cytotoxicity by directly targeting mitochondria in melanoma tissues and it may be a suitable candidate for novel drug
treatment of malignant melanomas.- انتشار مقاله: 16-12-1396
- نویسندگان: Fatemeh Zangeneh,Amir Vazirizadeh,Mohammad Reza Mirshamsi,Amir Fakhri,Mehrdad Faizi,Jalal Pourahmad
- مشاهده