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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Dog,Seroprevalence,Avian H9N2 Influenza Virus,Hemagglutination Inhibition
- چکیده:
- چکیده انگلیسی: Influenza is a highly contagious zoonotic disease in the world. Avian H9N2 influenza virus is a significant pandemic pathogen widely distributed throughout the world. Pet ownership has been documented as a risk factor for infection transmission to human. Considering major public health concern, the prevalence of antibodies against avian H9N2 influenza virus was evaluated in 170 serum samples of dogs by hemagglutination inhibition assay. This study is the first survey to assess the epidemiology of avian H9N2 influenza virus infection in dogs in Kerman, southeast of Iran. Out of 170 samples, 65 (38.23%) were positive for H9N2. Antibodies were higher in farm dogs that were kept with other animals and also in dogs were fed a raw diet. These findings emphasize the importance of close attention to these populations for control and prevention programs. It is important to reduce infection burden, especially in regions with widespread distribution of H9N2.
- انتشار مقاله: 22-03-1397
- نویسندگان: Mehdi Saberi,Hadi Tavakkoli,Ali Najmaddini,Mahdieh Rezaei
- مشاهده
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Dog,Diaphragmatic hernia,Congenital defect
- چکیده:
- چکیده انگلیسی: A one-month-old male terrier dog was referred in shock status with a history of anorexia, tachypnea, abdominal distention and progressive weight loss. Auscultation of right side of the lungs found enhanced respiratory noises. The thorough auscultation of the opposite side of the chest revealed the presence of typical intestinal sounds. Cardiac auscultation revealed muffled heart sounds and a diminished palpable precordial cardiac impulse was evident. The radiograph showed the presence of gas within the bowel in abrupt contrast to the adjacent structures of soft tissue opacity. Conservative treatment was failed and the animal died. At necropsy, cranial displacement of abdominal viscera into the pericardial sac was seen. A definitive diagnosis of peritoneopericardial diaphragmatic hernia was made. Although congenital pericardial diseases are rare in dogs, awareness of the clinical manifestation of these kinds of defects combined with early use of available imaging modalities can yield a preoperative diagnosis.
- انتشار مقاله: 02-02-1393
- نویسندگان: Reza Kheirandish,Mehdi Saberi,Dariush Vosough,Nasrin Askari
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Diclofenac,Cornea,Eye drop,Subconjunctival
- چکیده:
- چکیده انگلیسی: Objective- To compare the effects of topical eye-drop and subconjunctival administration of diclofenac on improvement of experimental corneal alkali wound in rabbits eyes.
Design- Experimental study.
Animal- fifteen rabbits.
Procedures- Alkali wounds were inflicted on the central corneas of 15 rabbits which were divided into three groups, A, B and C. A round 9 mm diameter paper filter that had been soaked in normal NAOH was positioned on all the rabbits’ right central cornea, to cause corneal wound. Group A and B were treated with Diclofenac eye-drop and subconjunctival injection of Diclofenac, respectively. And group C was indicated as a control group. After 21 days, all the right eyes were sent to laboratory for pathological analysis.
Result- Comparison between groups showed that groups A, and B, had significantly lower (P<0.05) discharge days than the control group. Duration of blepharospasm in groups A and B were significantly shorter than the control group (P<0.05). Corneal vascularization revealed significant differences between groups. Pair wise comparison determined that group B had a significantly lower degree of corneal vascularization than the control group. Stromal inflammation was most prominent in group A and control group. Statistical analysis showed significantly less inflammation in group B than the control group (P<0.05)
Conclusion and Clinical Relevance- Corneal ulcer is one of the most important and common disease of the anterior segment of the eye. Many drugs have been used to prevent and treat corneal ulcer complication. According to our results, topical usage of Diclofenac is easier and more practical than subconjunctival injection.- انتشار مقاله: 26-11-1393
- نویسندگان: Noushin Esmaeeli,Mehdi Saberi,Baharak Akhtardanesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Dog,Chemical Injuries,Corneal Burnt
- چکیده:
- چکیده انگلیسی: Case Description- A 3-month-old female intact Boxer dog was presented to the Veterinary Hospital of Shahid Bahonar University of Kerman with a complaint epiphora, photophobia and corneal opacity of both eyes.
Clinical Findings- Ophthalmic examination revealed mild blepharospasm and mucoid discharge of both eyes, with severe bilateral chemosis and conjunctival hyperemia. Severe corneal edema and a white haze to the corneal stroma in the affected area were also observed.
Treatment and Outcome- The dog was admitted to the hospital for 14 days for intensive topical treatment including atropine eye drop, acetylcysteine eye drop, tetracycline eye ointment, ciprofloxacin eye drop, diclofenac eye drop and topical autologous therapy, Three weeks after the injury, the epitheliums were covering entire of the corneal surface and the corneas were fluorescein negative.
Clinical Relevance- Chemical injuries of the eye rarely occur in the animals based on the scarcity of publications on the subject in the veterinary literature. In the present case, chemical eye burns due to accidental exposure to the povidone-iodine occurred during an elective surgery.- انتشار مقاله: 18-11-1393
- نویسندگان: Mehdi Saberi,Mahdieh Rezaei,Sharareh Aghaabbasi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: pH,Juglone,Aptamer,Aptasensor,Redox
- چکیده:
- چکیده انگلیسی: Electrochemical aptamer-based sensors attract a lot of interest as useful methods because of their low cost, accuracy, sensitivity, and simplicity. An electro-active redox molecule comprises the main part of the electrochemical-based sensors. Ferrocene is one of the most popular redox molecule used in biosensor fabrication. But, instability of ferrocenium ion in strong nucleophilic reagents and chloride containing solutions is one of the main problems of this redox molecule. In this study, Juglone is used as an effective quinone redox molecule for aptasensor designing in different pH ranges and different concentrations of chloride ion. The voltammetric studies showed that the electrochemical response of sensor increased by raising the buffer ionic concentration and the sensor accuracy in 7.0 to 8.0 pH range as well. According to the findings, Juglone could be used as an effective redox molecule in high concentrations of chloride containing solutions in the 7.0 pH.
- انتشار مقاله: 24-07-1390
- نویسندگان: Hossein Hamzeiy,Mehdi Saberian,Davoud Asgaria,Yadollah Omidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Helicobacter pylori,Polymorphism,Myeloperoxidase
- چکیده:
- چکیده انگلیسی: Background: Polymorphisms in the immune related genes are important in the clinical outcome of Helicobacter pylori infection. Myeloperoxidase -463 G/A polymorphism has been shown to reduce enzyme expression and activity.
Objective: the aim of the present study is to investigate the association of myeloperoxidase G-463A polymor-phism with clinical outcome of Helicobacter pylori infection.
Methods: two hundred and eighty five patients with positive culture of Helicobacter pylori from their gastric biopsies are included in this study. Human leukocyte DNA was extracted using salting out method and myeloperoxidase G-463A polymorphism was investigated by PCR-RFLP. All clinicopathological data were collected from individual records.
Results: When the patients were categorized according to the high (GG) and low + intermediate (AG+AA) genotypes of myeloperoxidase producers, there was a significant association between myeloperoxidase G-463A genotypes and clinical outcome of Helicobacter py-lori infection (p=0.006). In search for combined effect of cagA status and myeloperoxi-dase genotypes on clinical presentations, only in cagA- Helicobacter pylori infected pa-tients a significant association between myeloperoxidase genotypes and clinical out-come was found (p=0.0001). Also this association was found only in patients infected with vacA s1m1 genotype (p=0.008).
Conclusions: Our findings suggest that the mye-loperoxidase G-463A polymorphism is a host genetic factor which determines the clini-cal outcome of Helicobacter pylori infection. Moreover, the combination of host and bacterial genetics could provide a better understanding of clinical outcome after infec-tion with Helicobacter pylori.- انتشار مقاله: 17-05-1395
- نویسندگان: Eskandar Kamali-Sarvestani,Hadi Farsiani,Michel Shamoon Pour,Abdulah Bazargani,Kamran Lankarani,Ali-Reza Taghavi,Mehdi Saberifiroozi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Amygdala,coping strategies,Stress,Cortisol,Epinephrine,Rhesus macaque
- چکیده:
- چکیده انگلیسی: Objective(s): In the present study the effect of stress on monkeys that had learned to retrieve food from a five-chamber receptacle, as well as the relationship between their behavior and the serum cortisol and epinephrine levels and relative size of the amygdala was evaluated.
Materials and Methods: Six male rhesus monkeys were individually given access to the food reward orderly. They could easily retrieve the rewards from all chambers except for the chamber 4, which a brief, mild electric shock (3 V) was delivered to them upon touching the chamber’s interior. The coping behaviors were video-recorded and analyzed offline. Baseline serum cortisol and epinephrine levels were measured before the experiments using monkey enzyme-linked immunosorbent assay kit. One week after the behavioral experiment, the monkeys’ brains were scanned using magnetic resonance imaging under general anesthesia. The cross-sectional area of the left amygdala in sagittal plane relative to the area of the whole brain in the same slice was evaluated by the planimetric method using ImageJ software.
Results: Exposure to the distressing condition caused different behavioral responses. Monkeys with higher baseline levels of serum cortisol and epinephrine and larger amygdala behaved more violently in the face of stress, indicating adopting emotion-focused stress-coping strategies. Conversely, those with low plasma epinephrine, moderate cortisol, and smaller amygdala showed perseverative behavior, indicating a problem-focused coping style.
Conclusion: In dealing with the same stress, different responses might be observed from nonhuman primates according to their cortisol and epinephrine levels as well as their amygdala dimensions.- انتشار مقاله: 14-05-1396
- نویسندگان: Elaheh Tekieh,Esmail Riahi,Masoomeh Kazemi,Hedayat Sahraei,Hassan Tavakoli,Hamed Aliyary,Mostafa Hajinasrollah,Maryam Salehi,Gholamhossein Meftahi,Mehdi Saberi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Golestan Province,Wild Rodent,Leishmania infuntum,IFAT
- چکیده:
- چکیده انگلیسی: Leishmania infantum, an etiologic agent of zoonotic visceral leishmaniasis (VL), is spreading in Iran. Dogs are the main reservoirs but L.infantum has been detected in jackals, foxes and wild rodents too. Despite high number of rodents in the world, the exact role of them in epidemiology of VL is not clear. In recent years the number of sporadic cases of dogs and humans VL has been increased in arid and semi-arid areas of Golestan province. The aim of this study was to detect L.infantum antibodyin wild rodents of Turkemen Sahra district of Golestan province. 75 rodents were randomly trapped from 8 areas and after identification of the genus and species, blood was collected by cardiac puncture. Indirect immunofluorescence antibody test (IFAT) was performed on serum samples of captured rodents. % 8 of serum samples which belonged to Mus musculus population were positive. Results of current study highlight the possible role of wild rodents in the spreading of L.infantum in ecosystem. More epidemiological and molecular study is recommended to imagine the exact role of wild rodents in life cycle of L.infuntum in Golestan province.
- انتشار مقاله: 13-03-1392
- نویسندگان: Somayeh Namroodi,Mehdi Saberi
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Substance-related disorders,Death
- چکیده:
- چکیده انگلیسی: Background: The Ministry of Health and Medical Education (MOHME) and the Legal Medicine Organization
(LMO) are the 2 death registration systems in Iran for registering drug-related deaths. The aim of the present
study was to assess the number of undercount and the overlap between the deaths registered by the 2 sources.
Methods: In this descriptive study, according to the 10th revision of the International Classification of
Diseases (ICD-10), the registered data on drug-related deaths in the years 2014-2016, as recorded by the
MOHME and the LMO, were collected and the number of deaths was estimated using 2-source capturerecapture method and Excel and SPSS software.
Findings: The total number of drug-related deaths, as registered by the 2 sources, was 8639 during the
3 years. A major part of the drug-related deaths (75% of the data) had been registered by the LMO and only
25% of deaths had been registered by the MOHME. There was also a small overlap (7.7% of deaths) between
the data from the 2 sources. The final estimation from the capture-recapture model and analysis of sensitivity
showed that, during the 3 years, the total number of drug-related deaths was 14517 [95% confidence interval
(CI):14498-14558]. Based on the complete overlap assumption and 50% of unidentified individuals in the
2 sources, the number of deaths was estimated at 11341 and 12418, respectively. The largest number of
drug-related deaths had occurred within the age range of 25-39 years and in men. Kermanshah, Hamedan,
and Zanjan Provinces (Iran) had the largest number of cumulative incidences of drug-related deaths. Based
on the data provided by the MOHME, the most common cause of death was Methadone poisoning.
Conclusion: There was a small overlap between the MOHME and the LMO in the registration of drug-related
deaths. Failure to enter accurate and correct information has led to miscalculations of these deaths in Iran
- انتشار مقاله: 16-02-1399
- نویسندگان: Masoudeh Babakhanian,Mehran Zarghami,Abbas Alipour,Ardeshir Khosravi,Seyed Saeed Hashemi-Nazari,Mehdi Saberi,Mohammad Reza Ghadirzadeh
- مشاهده