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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Pathogenic bacteria,Thymus vulgaris,Biofilm,antimicrobial effect
- چکیده:
- چکیده انگلیسی: Objective: Microorganisms are responsible for many problems in industry and medicine because of biofilm formation. Therefore, this study was aimed to examine the effect of Thymus vulgaris (T. vulgaris) extracts on the planktonic form and biofilm structures of six pathogenic bacteria.
Materials and methods: Antimicrobial activities of the plant extracts against the planktonic form of the bacteria were determined using the disc diffusion method. MIC and MBC values were evaluated using macrobroth dilution technique. Anti-biofilm effects were assessed by microtiter plate method.
Results: According to disc diffusion test (MIC and MBC), the ability of Thymus vulgaris (T. vulgaris ) extracts for inhibition of bacteria in planktonic form was confirmed. In dealing with biofilm structures, the inhibitory effect of the extracts was directly correlated to their concentration. Except for the inhibition of biofilm formation, efficacy of each extract was independent from type of solvent.
Conclusion: According to the potential of Thymus vulgaris (T. vulgaris ) extracts to inhibit the test bacteria in planktonic and biofilm form, it can be suggested that Thymus vulgaris(T. vulgaris )extracts can be applied as antimicrobial agents against the pathogenic bacteria particularly in biofilm forms.- انتشار مقاله: 08-03-1393
- نویسندگان: Zinab Mohsenipour,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial activity,Pseudomonas aeruginosa,Zinc oxide nanoparticles,Solanum nigrum
- چکیده:
- چکیده انگلیسی: Background and aims: The purpose of this study was to evaluate the antimicrobial effects of zinc oxide nanoparticale and extract of Solanum nigrum on Pseudomonas aeruginosa bacteria isolated from clinical specimens.
Methods: Zinc oxide was purchased from the market. 20g of the dried and pounded leaves of S. nigrum was used and its extract was prepared in rotary device. 12 isolates of P. aeruginosa were isolated from referred patients to hospital Zabol. Finally, 12 isolates were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of Zinc oxide and extract plant against P.aeruginosa were evaluated using micro broth dilution method.
Results:The highest inhibitory concentration for P. aeruginosa is 1500 μg/ml, with four bacterial strains being inoculated. Also, the results showed that the highest drainage concentration was 3000 μg/ml, which two strains were inhibited in this concentration and the lowest trap concentration was 93 μg/ml. The lowest inhibitory concentration of extract plant was 0.62 mg / ml, with only one strain being inhibited in this concentration. The highest inhibitory concentration for P. aeruginosa is 40 mg / ml, with four bacterial strains being inoculated.
Conclusion:The results showed that zinc oxide and extract of Solanum nigrum have a good antimicrobial activity on the bacterium and increases the concentration of antimicrobial activity.- انتشار مقاله: 18-07-1396
- نویسندگان: Khadijeh Rezaie.Keikhaie,Gholamreza Bagheri,Saeide Saeidi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial,Plant extract,C. colocynthis,S.aureus
- چکیده:
- چکیده انگلیسی: Background and aims: Staphylococcus aureus and anaerobic gram-positive cocci, are medically the most important species in the genus Staphylococcus. Sometimes, the bacteria are called S. aureus. The constantly growing antibiotic-resistant bacterium is very important in treatments. The aim of this study is to evaluate the antimicrobial activity of ethanol extract from Citrullus colocynthis against the S. aureus resistant to antibiotics.
Methods: S. aureus strains were isolated from the city of Zabol and C.colocynthis extract was prepared using rotary devices and the MIC and MBC were determined by microdilution method.
Results: The results of this study showed that the extract from C. colocynthis inhibits the growth of bacteria in different concentrations, and that despite the relative strength of most species with different concentrations, the highest sensitivity was observed in concentrations of 10 and 20 mg/ml, in which is 100% bacteria were gone. C. colocynthis extract at a concentration of approximately 5 mg /ml had the highest inhibitory effect.
Conclusion: The results showed high antimicrobial effects of ethanol extract of C. colocynthis, which can offer appropriate drug therapy.- انتشار مقاله: 28-01-1396
- نویسندگان: Khadije Rezaie Keikhaie,Saeedeh Ghorbani,Zahra Hosseinzadeh,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial activity,Withania somnifera,Antimicrobial effects,Ultrasonic Waves
- چکیده:
- چکیده انگلیسی: Background and aims: Herbal medicines has been a major remedy in traditional medical systems from thousands of years and made a great contribution in maintaining human health and in preventing many infectious diseases.
K. pneumonia is a bacterial negative intestinal bacillus and a member of Enterobacteriaceae family. This paper aims at investigating the antimicrobial effects of the methanol extract of wind cheese prepared by ultrasonic waves on the K. pneumonia
Methods: All 12 isolates of K. pneumoniae isolated from urine culture of hospitalized patients (Amir al-Mu'minin Hospital, Zabol, southeastern of Iran) suffered from urinary tract infection during the years 2013 and 2014. In this study, the extract of W. somnifera obtained by ultrasonic waves. Then the minimum inhibitory concentrations and minimum bactericidal concentration were investigated to characterize the antibacterial activities of this extract
Result: The result showing that plant extracts from W. somnifera had inhibitory effect against K. pneumoniae. The MIC values were also determined against all the tested bacteria. The highest MIC values of extract were found to be 5mg/ml against K. pneumoniae and four of MIC value for K. pneumoniae was 2.5 mg/ml. (p<0.005).
Conclusion: The obtained results showed that extraction by using ultrasonic method improved the efficiency and amount of the antimicrobial effects of the plant. So, ultrasonic waves can be used as a stimulus for increasing the antimicrobial effects of the plant.- انتشار مقاله: 22-11-1395
- نویسندگان: Mohammad Amin Masshady,Javad Roustakhiz,Saphora Baz,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial activity,Human pathogen,Minimum inhibitory concentration
- چکیده:
- چکیده انگلیسی: Background and aims: Disease causing bacteria have always been considered a major cause of morbidity and mortality in humans. The appearance of resistant microorganisms paved the way to the occurrence of infections that are only treated by a limited number of antimicrobial agents. The present study was, the antimicrobial effects of Rosmarinus officinalis and Glycyrrhiza glabra extract against some pathogens.
Methods: In this study, the antibacterial activity using 9 Gram-positive and Gram-negative bacterial strains includes: Streptococcus pyogenes ATCC® 19615, Streptococcus pneumoniae ATCC 49619, S. saprophyticus ATCC®15305, Hafnia alvei ATCC 51873, Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratia marcescens ATCC 274 and Staphylococcus aureus ATCC® 25923 with micro dilution methods was studied. The MIC, MBC were studied also, resistance of these bacteria to standard antibiotics such as erythromycin, cefixime, ceftazidime, tetracycline, ampicillin and amikacin were compared.
Results: In this study, the minimum inhibitory concentration (MIC) was used. The levels of MIC of R. officinalis were in ranges from 6.25 to 25 mg/ml. The highest MIC value was observed at 25 ppm against S. pyogenes, S. pneumoniae and P. mirabilis and the levels of MIC of G. glabra were in ranges from 6.25 to 12.5 ppm. The highest MIC value was observed at 12.5 ppm against S. pyogenes, S. pneumoniae, P. mirabilis and S. marcescens.
Conclusion: In important human pathogens, drug resistance is increasing according to the results of this study, and may be proposed that this plant can be used as a drug. It can be a good way to replace herbs with chemical drugs.- انتشار مقاله: 02-10-1395
- نویسندگان: Somay Jahani,Zahra Hoaaeinzadeh,Zahra Shahi,Maryam Sheykhzade Asadi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Extract plant,Withania somnifera,Antifungal effects
- چکیده:
- چکیده انگلیسی: Background and aims: Withania somnifera (W. somnifera), commonly known as Ashwagandha, is an important medicinal plant that has been used in Ayurvedic and indigenous medicine for over 3,000 years. Candidiasis is one of the most common opportunistic fungal diseases in humans. In fact, the most important fungal disease in women is vaginal candidiasis. This study aimed to investigate the antifungal effects of the extracts of the W. somnifera on Candida albicans (C. albicans).
Methods: In this experimental research, 9 vaginal samples were collected using the sterile swap and Falcon tube by the gynecological specialists. The extracts of the shallot and artichoke were prepared using a rotary device. The inhibitory concentration against C. albicans was determined using incubation in media.
Results: The results of this study suggested that the minimum inhibitory concentration (MIC) against C. albicans, which is equivalent 50 ppm to 250 ppm has the highest concentration of inhibitor.
Conclusion: The results of this study showed that the antifungal activity of wind cheese against C. albicans is good, so it can be used as a drug to treat infections caused by C. albicans.- انتشار مقاله: 09-12-1394
- نویسندگان: Fereshteh Javadian,Zahra Sepehri,Saeideh Saeidi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antimicrobial activity,Artemisia santonica,Pathogenic bacteria,Drug resistance
- چکیده:
- چکیده انگلیسی: Background and aim: For rapid growing drug resistance among pathogenic microorganisms, it is essential to find new compounds with antimicrobial activity. Medicinal herbs application in traditional treatment for thousand years, make this natural compound to the best choice for antimicrobial studies. The aim of this study is to determine the antimicrobial activity of Artemisia against some important pathogenic bacteria. Material and Method: In the present study, antimicrobial activity of ethanolic and methanolic extracts of A. santonica against four pathogenic bacteria was evaluated. A. santonica dry powder was extracted with ethanol and methanol solvents in maceration method. Assessment of antimicrobial efficacies of extracts and MIC were conducted by disk diffusion manual and macro broth dilution method, respectively. Results: Both ethanolic and methanolic extracts could inhibit microorganism in this test. Staphylococcus aureus with MIC=1.56 mg/ml indicates more sensitive to the Artemisia. In addition, Bacillus cereus showed medium sensitivity. E.coli and Pseudomonas aeruginosa became involved in less sensitivity to the A. santonica extracts. Conclusion: The result of this study confirmed that A. santonica extracts were the best inhibitory effect on S. aureus. Then it can be concluded that by using herbal plant against antibiotics some antibiotic resistant problem can be resolved.
- انتشار مقاله: 24-06-1394
- نویسندگان: Mehdi Hassanshahian,Farshid Khosravi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial,Ethanol extract,Mentha Longifolia,Salmonella typhimurium
- چکیده:
- چکیده انگلیسی: Background and aims: Traditionally, plants have long been applied in the treatment of various diseases throughout the history of human life. The antimicrobial activity of plant extracts in the laboratory is studied on Gram positive and negative strains of human infectious agents. The aim of this study was to evaluate the antibacterial activity of Mentha longifolia against important human pathogens. Methods: This was an experimental study and no statistical method was used. The antimicrobial effect of ethanol extracts of Mentha longifolia were investigated on Salmonella typhimurium were determined using broth microdilution method. The broth microdilution method was used to determine the Minimal Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). All tests were performed in Mueller Hinton broth supplemented with Tween 80 at a final concentration of 0.5%. Results: The results showed that the lowest MIC concentration was 5 mg/ml that 1 strain of Salmonella was inhibited. The highest and the lowest MBC values of extract were 40 and 10 mg/ml, respectively. Conclusion: Since the antibiotic resistant bacteria are increasing in the world, replacing the chemical and synthetic antibiotics with natural products is essential. It can be concluded from the results of this study that using herbal plant instead of antibiotics can resolve some antibiotic resistant problems.
- انتشار مقاله: 03-04-1394
- نویسندگان: Forouzan Heydari,Saeedeh Saeedi,Mehdi Hassanshahian
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial,Ethanol extract,Mentha Longifolia,Salmonella typhimurium
- چکیده:
- چکیده انگلیسی: Background and aims: Traditionally, plants have long been applied in the treatment of various diseases throughout the history of human life. The antimicrobial activity of plant extracts in the laboratory is studied on Gram positive and negative strains of human infectious agents. The aim of this study was to evaluate the antibacterial activity of Mentha longifolia against important human pathogens. Methods: This was an experimental study and no statistical method was used. The antimicrobial effect of ethanol extracts of Mentha longifolia were investigated on Salmonella typhimurium were determined using broth microdilution method. The broth microdilution method was used to determine the Minimal Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). All tests were performed in Mueller Hinton broth supplemented with Tween 80 at a final concentration of 0.5%. Results: The results showed that the lowest MIC concentration was 5 mg/ml that 1 strain of Salmonella was inhibited. The highest and the lowest MBC values of extract were 40 and 10 mg/ml, respectively. Conclusion: Since the antibiotic resistant bacteria are increasing in the world, replacing the chemical and synthetic antibiotics with natural products is essential. It can be concluded from the results of this study that using herbal plant instead of antibiotics can resolve some antibiotic resistant problems.
- انتشار مقاله: 03-04-1394
- نویسندگان: Forouzan Heydari,Saeedeh Saeedi,Mehdi Hassanshahian
- مشاهده