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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Alkaline protease,Exiguobacterium,One-factor-at-a-time strategy,Response surface methodology
- چکیده:
- چکیده انگلیسی: Improvement of alkaline protease production, one of the most important types of industrial biocatalysts, is essential for commercial purposes. The present study’s aim was to increase alkaline protease production of MSB42, a newly isolated strain of Exiguobacterium sp., through the rotatable central composite design (RCCD). In this regard, parameters affecting MSB42 enzyme production were chosen based on the results of preliminary one-factor-at-a-time (OFAT) experiments. According to the results of RCCD, the enzyme production showed its maximum level (198.48 U/ml) in the presence of 3% w/v glucose, 3% w/v peptone, at 37 °C and pH 9. Overall, the enzyme production was 2 and 3.24 times more than OFAT experiments (98.69 U/ml) and unoptimized conditions (61.29 U/ml), respectively. A polynomial model of MSB42 alkaline protease production was obtained using the experimental date and based on the significant terms. The protease gene of this strain was also amplified using the designed primers (873 bp), and based on the sequencing results, was found to be closely related to the M6 family of metalloprotease domain.
- انتشار مقاله: 08-03-1398
- نویسندگان: Matia Sadat Borhani,Zahra Etemadifar,Davood Biria,Eisa Jorjani
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Biodesulfurization,4S Pathway,dsz genes,Genetic Engineering,Recombinant Strains
- چکیده:
- چکیده انگلیسی: Biodesulfurization could be a beneficial method in the industry for lowering the sulfur content of crude oil and could improve the efficiency of currently and costly used method, i.e., hydrodesulfurization. But, to achieve this goal, it is critical to enhancing the BDS rate using genetics and other omics approaches. The reason attributed to the fact that the desulfurization activity of currently available natural biocatalysts does not meet the needs of the petroleum industry. Based on the expression of the 4S pathway genes in several heterologous hosts with no ability of desulfurization, the involved genes are ideal targets for enhancement through genetics or metabolic engineering approaches. This review provides an overview of the reported solutions concerning identification of new desulfurizing genes and their regulation, elimination of the inhibitory effects of end products, solving the problem of mass transfer, increasing the production, specific activity, and stability of the involved enzymes, and enhancing the resistance of recombinant strains to the biodesulfurization conditions. Also, the results of some bioinformatics studies which can help in the genetic engineering of desulfurizing strains were provided. Overall, genetic engineering and bioinformatics techniques can be effective in solving the problems of the biodesulfurization process in the near future as a complementary method of hydrodesulfurization.
- انتشار مقاله: 09-06-1398
- نویسندگان: Matia Sadat Borhani,Zahra Etemadifar
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Alkaline protease,Exiguobacterium,One-factor-at-a-time strategy,Response surface methodology
- چکیده:
- چکیده انگلیسی: Improvement of alkaline protease production, one of the most important types of industrial biocatalysts, is essential for commercial purposes. The present study’s aim was to increase alkaline protease production of MSB42, a newly isolated strain of Exiguobacterium sp., through the rotatable central composite design (RCCD). In this regard, parameters affecting MSB42 enzyme production were chosen based on the results of preliminary one-factor-at-a-time (OFAT) experiments. According to the results of RCCD, the enzyme production showed its maximum level (198.48 U/ml) in the presence of 3% w/v glucose, 3% w/v peptone, at 37 °C and pH 9. Overall, the enzyme production was 2 and 3.24 times more than OFAT experiments (98.69 U/ml) and unoptimized conditions (61.29 U/ml), respectively. A polynomial model of MSB42 alkaline protease production was obtained using the experimental date and based on the significant terms. The protease gene of this strain was also amplified using the designed primers (873 bp), and based on the sequencing results, was found to be closely related to the M6 family of metalloprotease domain.
- انتشار مقاله: 08-03-1398
- نویسندگان: Matia Sadat Borhani,Zahra Etemadifar,Davood Biria,Eisa Jorjani
- مشاهده