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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Cat,Leptospira,Mashhad,Sero-epidemiology
- چکیده:
- چکیده انگلیسی: The importance of cats in the Leptospira epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. The purpose of this survey was to determine the prevalence of Leptospira infection in shorthair cats in different location of Mashhad, Iran. Totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households, stray and cats which lived in industrial dairy cattle herds of Mashhad, Iran, respectively. Sera were tested with seven live Leptospira antigens using microscopic agglutination test (MAT). Sera with 50.00% agglutination at the dilution of ≥ 1/100 were considered as positive samples. Agglutination at dilutions of < 1/100 considered as suspected to Leptospira infection. Overall, 19 (12.92%) out of 147 cats showed reaction in MAT. The seroprevalence at a titer ≥ 1:100 and < 1:100 were 10 (6.80%) and 9 (6.12%), respectively. Serum samples showed positive reaction against Leptospira intterogans hardjo (no = 10; 52.63%), pomona (no = 5; 26.31%) and icterohaemorrhagiae (no = 4; 21.05%). Eight cats (42.10%) belong to dairy cattle herds had the most infection only by L. I. hardjo with 1:200 titer. There were no significant differences among the weight‚ age and sex of infected cats. However, there were significant differences between the infected cats in dairy cattle herds and the cats in the urban area (p < 0.05). It is concluded that cats can be infected by Leptospira spp. especially in commercial dairy cattle herds. Cats can be considered as a sanitation hazards in the area for this zoonotic disease.
- انتشار مقاله: 20-08-1394
- نویسندگان: Massoud Talebkhan Garoussi,Mohsen Mehravaran,Gholamreza Abdollahpour,Javad Khoshnegah
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Spermatogonial stem cell,CSF1,bovine spermatogonia,SSC coculture,SSC niche
- چکیده: زمینه مطالعه: سلولهای بنیادی اسپرماتوگونی (SSCs) گروه کوچکی از سلولهای اسپرماتوگونی نوع A هستند که می توانند سلولهایی کاملاً مشابه خود ایجاد نموده (خودسازی) و همچنین تمایز یابند و در پایان روند اسپرماتوژنز، اسپرم را تولید نمایند. فرآیند خودسازی SSCs به طور کامل شناخته نشده است. برای فراهم شدن امکان مطالعه خصوصیات و عملکرد این سلولها که منجر به خودسازی و تمایز آنها می گردد، دسترسی به تعداد کافی از آنها ضروری است. هدف: در این مطالعه اثر دوزهای مختلف CSF1 روی کلونیزاسیون SSCs در همکشتی با سلولهای سرتولی بررسی شد. CSF1 از عواملی است که پیشنهاد شده ممکن است در SSC niche (جایگاه قرارگیری SSCs در لوله های سمینیفروس) که در آن خودسازی به تمایز برتری دارد، نقش داشته باشد. روش کار: تعداد و مساحت کلونی های اسپرماتوگونی ایجاد شده در حضور دوزهای متفاوت CSF1 (0، 10، 50 و 100 ng/ml) طی روزهای 4، 7 و 11 کشت ارزیابی گردید. توسط رنگ آمیزی ایمیونوسیتوفلورسنت علیه مارکرهای OCT4 و vimentin ماهیت SSCs و سلولهای سرتولی تاٌیید شد. نتایج: تعداد کلونی ها از روز 4 تا روز 11 در تمامی گروهها مستقل از حضور و یا دوز CSF1 به شکل معنی داری افزایش نشان داد. مشاهده گردید که تعداد کل کلونی ها و مجموع مساحت آنها در گروههای SCF1 10 و 50 ng/ml نسبت به گروههای CSF1 100 و 0 (کنترل) بالاتر است اما این تقاوت تنها در مورد مقایسه مجموع مساحت کلونی ها بین گروه CSF1 10 ng/ml و کنترل و تنها در روز 4 معنی دار بود (p
- چکیده انگلیسی: BACKGROUND: Spermatogonial stem cells (SSCs) are infrequent
self-renewing cells among the type A spermatogonia
within the seminiferous tubules and are the basis of spermatogenesis
in mammalian testis. An adequate number of SSCs is a
primary requirement for the study of their behavior, regulation, and
further biomanipulation. OBJECTIVES: In this paper, we studied
the development of the primary co-cultures of type A spermatogonia
and prepubertal bovine sertoli cells in the presence of Colony
Stimulating Factor 1 (CSF1), a potential contributor in the SSC
niche. METHODS: The effect of different concentrations of CSF1
(0, 10, 50 and 100 ng/mL) on the colonization activity of spermatogonial
cells was assessed 4, 7 and 11 days after the beginning of the
culture by counting the total number of colonies and measuring their
area in each group of the present experiment. Immunofluorescent
staining against OCT4 and vimentin led to the confirmation of the
nature of both the SSCs and sertoli cells. RESULTS: Results showed
that the total number of colonies from day 4 to 11 increased
significantly in all groups, independent of CSF1 concentration. In
addition, the total number and total area of colonies were higher (not
significant) in 10 and 50 ng/mL CSF1 treatments than the control
and 100 ng/mL CSF1 groups in all the three evaluations during the
experiment. However, this difference was only significant (p<0.05)
between the total area of colonies in the control and 10 ng/mLCSF1
groups at day 4 of co-culture. CONCLUSIONS:It was concluded that
CSF1 can be a suitable growth factor for improving SSCs colonization
in vitro, particularly during the first days of culture where
accompanying sertoli cells still have not proliferated sufficiently to
support the propagating spermatogonial cells.- انتشار مقاله: 15-08-1391
- نویسندگان: Shiva Shafiei,Parviz Tajik,Hamid Ghasemzadeh-nava,Mansoureh Movahedin,Massoud Talebkhan Garoussi,Babak Qasemi-Panahi,Peyman Rahimi Feyli
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cattle,Bulk milk,BVD virus,Nested RT- PCR
- چکیده:
- چکیده انگلیسی: Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy cattle. In this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against BVDV. RNA was extracted from somatic cell pellets of bulk milk tank samples. Oligonucleotide primers were selected based on the 5´ untranslated region of the BVD virus genome. BVD virus was detected in 2 (11.1%) out of 18 samples, representing 742 lactating cows. These results indicate that nested RT-PCR analysis of bulk milk samples may provide a rapid and sensitive screening method for the detection of BVDV infections in non-vaccinated dairy cattle herds.
- انتشار مقاله: 09-06-1394
- نویسندگان: Massoud Talebkhan Garoussi,Mohammad Reza Bassami,Seyed Ehsan Afshari
- مشاهده