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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: miR-9,miR-7,miR-23a,miR-23b,miR-96,miR-204,miR-211,RGS5
- چکیده:
- چکیده انگلیسی: Objective(s):An earlier meta-analysis on gene expression data derived from four microarray, two cDNA library, and one SAGE experiment had identified RGS5 as one of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (TM) cells by all studies. RGS5 encodes regulator of G-protein signaling-5. The TM tissue is the route of aqueous fluid outflow, and is relevant to the pathology of glaucoma. MicroRNAs constitute the most recently identified components of the cellular machinery for gene regulation in eukaryotic cells. Given our long standing interest in glaucoma, we aimed to identify miRNAs that may target RGS5.
Materials and Methods: Eight miRNAs were selected for study using bioinformatics tools and available data on miRNAs expressed in the eye. Their effects were assessed using the dual luciferase assay. 3'-UTR segments of RGS5 mRNA were cloned downstream of a luciferase coding gene in psiCHECK2 vectors, and these were co-transfected with each of the miRNAs into HEK293 cells.
Results: The outcomes evidenced that one or more of the segments are in fact targeted by miR-7, miR-9, miR-96, miR-23a, miR-23b, miR-204, and miR-211. Down regulations by the miRNAs were statistically significant. The effect of miR-204 is considered particularly important as this miRNA is well known to regulate eye development and to affect multiple ocular functions.
Conclusion: Our results justify further studies on regulation of RGS5 expression and RGS5 downstream functions by these miRNAs.- انتشار مقاله: 06-12-1393
- نویسندگان: Amir Banaei-Esfahani,Hamidreza Moazzeni,Pooya Naseri Nosar,Sadaf Amin,Ehsan Arefian,Masoud Soleimani,Shahin Yazdani,Elahe Elahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Tissue engineering,Adipose-derived stem cells,Cardiomyocyte differentiation,PCL nanofibers
- چکیده:
- چکیده انگلیسی: Objective(s):Cardiomyocytes have small potentials for renovation and proliferation in adult life. The most challenging goal in the field of cardiovascular tissue engineering is the creation of an engineered heart muscle. Tissue engineering with a combination of stem cells and nanofibrous scaffolds has attracted interest with regard to Cardiomyocyte creation applications. Human adipose-derived stem cells (ASCs) are good candidate for use in stem cell-based clinical therapies. They could be cultured and differentiated into several lineages such as cartilage, bone, muscle, neuronal cells, etc.
Materials and Methods:In the present study, human ASCs were cultured on random and aligned polycaprolactone (PCL) nanofibers. The capacity of random and aligned PCL nanofibrous scaffolds to support stem cells for the proliferation was studied by MTT assay. The cardiomyocyte phenotype was first identified by morphological studies and Immunocytochemistry (ICC) staining, and then confirmed with evaluation of specific cardiac related gene markers expression by real-time RT-PCR.
Results:The proliferation rate of ASCs on aligned nanofibrous PCL was significantly higher than random nanofibrous PCL. ICC and morphological studies results confirmed cardiomyocyte differentiation of ASCs on the nanofibrous scaffolds. In addition, the expression rate of cardiovascular related gene markers such as GATA-4, α-MHC and Myo-D was significantly increased in aligned nanofibrous PCL compared with random nanofibrous PCL.
Conclusion:Our results show that the aligned PCL nanofibers are suitable physical properties as polymeric artificial scaffold in cardiovascular tissue engineering application.- انتشار مقاله: 20-09-1393
- نویسندگان: Raheleh Safaeijavan,Masoud Soleimani,Adeleh Divsalar,Akram Eidi,Abdolreza Ardeshirylajimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytarabine,reactive oxygen species,Olea,Sunphenon,Stomach neoplasms
- چکیده:
- چکیده انگلیسی: Background: According to the epidemiological studies, consuming olive products can decrease the incidence of the different types of cancers mostly due to the high anti-oxidant properties of their polyphenolic compounds.
Objectives: To evaluate the anti-oxidant and anti-proliferative potentials of the olive fruits total polyphenols on the gastric adenocarcinoma MKN45 cells in comparison to the normal Hu02 cells.
Materials and Methods: The total phenolic content of the olive fruits and radical scavenging activity were determined by Folin and 2,2-diphenyl-1-picrylhydrazyl (DPPH) tests respectively. MTT assay was performed for the evaluation of the cell viability. Intracellular reactive oxygen species (ROS) level was measured using DCFH-DA. Statistical analysis was performed using SPSS 16 statistical software.
Results: Treatment of the MKN45 cells with the phenolic compounds extracted from olive fruits decreased growth and viability of the cells in a dose- and time-dependent manner. In addition, treatment of the MKN45 cells with a combination of the phenolic compounds extracts and cytarabine further decreased cell compared to monotherapy of the cells with each compound alone. Mechanistically, we showed that the anti-cancer effects of the olive polyphenols in the MKN45 cells are mediated through depletion of ROS. Similarly, polyphenolic extracts were found to decrease ROS level in the normal cells at the concentrations of 500 and 1000 μg.mL-1 and short treatment times (6 h), but the viability of these cells did not significantly change. At high concentrations (2000 μg.mL-1) of the phenolic extracts or at longer times of incubation (12 h), however, both ROS levels and the viability of the cells were significantly decreased in the normal cells.
Conclusions: The olive fruits polyphenolic extract modulates ROS levels and selectively targets cancerous cells at low concentrations. Also, the effects of cytarabine could be potentiated by the olive fruits polyphenols. Thus, for a combined protocol of cancer cell therapy, olive fruit polyphenolic compound could be proposed as a proper candidate.- انتشار مقاله: 13-04-1396
- نویسندگان: Alireza Amiri-nowdijeh,Mohammad Amin Moosavi,Simzar Hosseinzadeh,Masoud Soleimani,Farzaneh Sabooni,Mehdi Hosseini-Mazinani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Mineralization,perfusion bioreactor,Electrospun scaffolds,Multilayer construct,Osteogenic differentiation
- چکیده:
- چکیده انگلیسی: Background: Monolayer electrospun scaffolds have already been used in bone tissue engineering due to their high surface-to-volume ratio, interconnectivity, similarity to natural bone extracellular matrix (ECM), and simple production. Objectives: The aim of this study was to evaluate the dynamic culture effect on osteogenic differentiation and mineralizationi into a compact cellular multilayer nHA-PCL electrospun construct. The dynamic culture was compared with static culture. Materials and Methods: The calcium content, alkaline phosphatase (ALP) activity and cell viability were investigated on days 3 and 7. Results: When the dynamic culture compared to static culture, the mineralization and ALP activity were increased in dynamic culture. After 7 days, calcium contents were 41.24 and 20.44 mg.(cm3)-1, and also normalized ALP activity were 0.32 and 0.19 U.mg-1 in dynamic and static culture, respectively. Despite decreasing the cell viability until day 7, the scanning electron microscopy (SEM) results showed that, due to higher mineralization, a larger area of the construct was covered with calcium deposition in dynamic culture. Conclusions: The dynamic flow could improve ALP activity and mineralization into the compact cellular multilayer construct cultured in the perfusion bioreactor after 7 days. Fluid flow of media helped to facilitate the nutrients transportation into the construct and created uniform cellular construct with high mineralization. This construct can be applied for bone tissue engineering.
- انتشار مقاله: 06-08-1394
- نویسندگان: Maliheh Yaghoobi,Sameereh Hashemi-Najafabadi,Masoud Soleimani,Ebrahim Vasheghani-Farahani,Seyyed Mohammad Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: carbonates,Transfusion,red blood cells,Methoxy Polyethylene Glycol
- چکیده:
- چکیده انگلیسی: Background: Host immune system response against blood group antigens is a major problem in blood transfusions, especially for thalassemic patients. Thus, an approach was proposed coating the red blood cell (RBC) surface by polyethylene glycol. Objectives: This study aimed to obtain the optimal simultaneous camouflge of the major and minor antigens by activated methoxy polyethylene glycol (mPEG) with succinimidyl valerate (SVA) and succinimidyl carbonate (SC), separately. Materials and Methods: The degree of RBC agglutination by antibodies against the major and minor blood groups was used as a surrogate measurement for quantitative assessment of the effctiveness of the surface coating. Also, the RBC morphology was assessed using scanning electron microscope (SEM). In addition, to evaluate the host immune system response, the PEGylated RBCs were transferred between two diffrent mouse strains. Results: Statistical analysis of the results demonstrated that the optimal reaction conditions for simultaneous coating of the antigens by mPEG-SVA and mPEG-SC are as mPEG20 in the polymer mixture, 91.2 and 90.0%, and polymer concentration, 17.21 and 19.80 mg.mL-1, respectively. However, according to the SEM results, the maximum polymer concentration of 14.5 mg.mL-1 was suggested as the best condition for mPEG-SVA modifid human RBCs. Conclusions: It is concluded that the membrane PEGylation camouflges the blood group antigens. This effct is observed signifiantly for non-ABO/Rh(D) antigens. Also, it is found that the mPEG-SVA provide better coverage than mPEG-SC. The results of in vivo analysis showed that the immune reactions against PEGylated RBCs were considerably reduced, so that the levels of the relevant biochemical parameters in serum were similar to those of the normal hosts 24 hours after transfusion.
- انتشار مقاله: 11-10-1392
- نویسندگان: Zahra Gholami,Sameereh Hashemi Najafabadi,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Gum Tragacanth,Tyramine, Horseradish Peroxidase,In situ Forming Hydrogel,Enzyme Catalyzed Gelation,Biomedical applications
- چکیده:
- چکیده انگلیسی: Background: The excellent biocompatibility, biodegradability and biological properties of the hydrogels, fabricated using natural polymers, especially polysaccharides, are very advantageous for biomedical applications. Gum tragacanth (GT) is a heterogeneous highly branched anionic polysaccharide, which has been used extensively in food and pharmaceutical industries. Despite, its desirable properties, the potential biomedical applications of this natural gum have not been fully explored. In this study, an enzyme catalyzed in situ forming hydrogel, based on Iranian gum tragacanth (exudate of Astragalus fluccosus) was prepared and characterized for biomedical applications. Objectives: The main objective of the present study was to explore the feasibility of using tragacanth natural gum as a base for in situ-forming hydrogels in biomedical applications. Materials and Methods: First, tyramine (TA) was conjugated to the water-soluble part of GT (TGA) using aqueous-phase carbodiimide activation chemistry. Next, in situ forming hydrogel was prepared via an enzyme catalyzed coupling reaction in the presence of horseradish peroxidase (HRP) and H2O2. Gelation time, swelling/degradation behavior and mechanical properties of the hydrogel and cell viability of the encapsulated cells within these hydrogels were investigated. Results: The gelation time of the hydrogel was less than 30 seconds, which is very desirable for clinical applications. At concentrations ≤ 0.1% (w/v), both GT and TA-TGA showed no toxicity towards human mesenchymal stem cells (hMSCs) and Caco-2 cells. More than 90% of the encapsulated hMSCs in the hydrogels, which were prepared at H2O2 concentrations of less than 15.0 mM, remained viable after 2 hours of incubation. Conclusions: The TA-TGA conjugate can be gelled enzymatically in the presence of HRP and H2O2. This in situ forming hydrogel might be a desirable candidate for biomedical applications.
- انتشار مقاله: 08-08-1392
- نویسندگان: Moslem Tavakol,Ebrahim Vasheghani-Farahani,Masoud Soleimani,Mohammad Amin Mohammadifar,Sameereh Hashemi-Najafabadi,Maryam Hafii
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Human bone marrow mesenchymal stem cells,Drug metabolism,Glutathione S-transferases, cytochrome P450
- چکیده:
- چکیده انگلیسی: Currently several studies are being carried out on various properties of mesenchymal stem cells (MSCs)
however there are a few investigations about drug metabolizing properties of these cells. The aim of this
study was to measure the key factors involved in drug metabolism in human bone marrow MSCs. For this
purpose, cellular glutathione (GSH), glutathione Stransferase (GSTs) and cytochrome P450 class 3A4
(CYP3A4) were detected in these cells. Results showed that CYP3A4 and GSTA1-1 mRNA are not detectable in MSCs however mRNA specific for GSTP1-1 was considerably expressed in MSCs. GSH content and GST activity was also detected in MSCs. These data suggest that human bone marrow MSCs possess the drug metabolizing activity which may be useful in handling drugs and chemotherapeutic agents passing to the bone marrow.
- انتشار مقاله: 10-07-1391
- نویسندگان: Shahnaz Esmaeli,Abdolamir Allameh,Mohammad Sajad Emami Aleagha,Somaieh Kazemnejad,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: CD133+ cells,g-globin,Fetal hemoglobin,SCF,TGF-b
- چکیده:
- چکیده انگلیسی: Increased fetal hemoglobin (HbF) in b-globin gene disorders ameliorates the clinical symptoms of the underlying disease. 5-azacytidine, butyrate and hydroxyurea, have been shown to activate g-globin gene expression. It has also been found that hematopoietic growth factors can influence expression of g-globin in erythroid cultures and in animal models. This study was designed to evaluate the in vitro effects of the stem cell factor (SCF) and transforming growth factor-b (TGF-b) on g-globin gene reactivation of erythroid precursors derived from CD133+ cells in vitro. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis showed increased expression of the g-globin transcript in cell culture groups containing either TGF- b or SCF or both as compared to control (2.2-, 2.7- and 5.5-fold, respectively) (p<0.01). Production of HbF in a differentiated population was demonstrated using flow cytometry. The results of this study suggest that SCF and TGF-b warrant further evaluation as potential therapeutic drugs for the treatment of b-globin gene disorders.
- انتشار مقاله: 05-02-1394
- نویسندگان: Amir Atashi,Masoud Soleimani,Saeid Kaviani,Abbas Hajifathali,Ehsan Arefian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: mesenchymal stem cell,Angiogenesis,In vitro,Differentiation,Endothelial cell
- چکیده:
- چکیده انگلیسی: Human bone marrow derived mesenchymal stem cells (HBMSCs) have the potential to differentiate into cells such as adipocyte, osteocyte, hepatocyte and endothelial cells. In this study, the differentiation of hBMSCs into endothelial like-cells was induced in presence of vascular endothelial growth factor (VEGF) and insulin-like growth factor (IGF-1). The differentiated endothelial cells were examined for their ability to express VEGF receptor-2 (VEGFR2) and von willebrand factor (vWF). Then the cells were adopted to grow and develop capillary network in a semisolid gel matrix in vitro. The capillary network formation in a well of 24-well plate was found to be 85% in presence of VEGF (50ng/ml) and IGF-1 (20ng/ml) of the culture media. These data may suggest that the expression of endothelial markers in endothelial like-cells derived from hBMSCs is associated with their ability to form capillaries.
- انتشار مقاله: 15-02-1394
- نویسندگان: Maryam Jazayeri,Abdolamir Allameh,Masoud Soleimani,Seyed Hamid Jazayeri,Saeid Kaviani,Somaieh Kazemnejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Mesenchymal Stem Cells,Differentiation,Nanofiber,Scaffold,Hepatocyte
- چکیده:
- چکیده انگلیسی: In this present study, we examined the differentiation potential of human bone marrow derived mesenchymal stem cells (hBMSCs) into hepatocytes on a three-dimentional (3D) nanofibrous scaffold formed by Poly (ε-caprolactone) (PCL), collagen and polyethersulfone (PES). The nanofiber was prepared by the electrospining technique. HBMSCs were isolated using combining gradient density centrifugation with plastic adherence. Flow cytometric analysis was used to identify the isolated MSCs. The performance of the cells on the scaffold was evaluated by scanning electron microscopy (SEM) and MTT assay. The hBMSCs were then cultured in a hepatic differentiation medium containing hepatocyte growth factor (HGF), oncostatin M (OSM) and dexamethasone (DEX) for up to 21 days. The results showed that the isolated hBMSCs expressed specific markers such as CD44, CD166, CD105 and CD13. The integrity of the MSCs was further confirmed by their differentiation potential to osteogenic and adipogenic lineages. Scanning electron micrographs and MTT analysis revealed that the cells adhered and proliferated well on the nanofibrous hybrid scaffolds. Immunocytochemical analysis of albumin and a-fetoprotein (AFP) showed the accumulation of these markers in the differentiated cells on the scaffold. Hepatocyte differentiation was further confirmed by showing expression of albumin, AFP and cytokeratin-19 (CK-19) at mRNA levels in differentiated cells. In conclusion, the evidences presented in this study show that the engineered scaffold is promising for maintenance of hepatocyte-like cells suitable for transplantation.
- انتشار مقاله: 16-02-1394
- نویسندگان: Somaieh Kazemnejad,Abdolamir Allameh,Masoud Soleimani,Ahmad Gharehbaghian,Yousef Mohammadi,Naser Amirizadeh,Saeed Kaviani,Maryam Jazayeri,Maryam Amani
- مشاهده