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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Geopersia
- نوع مقاله: Journal Article
- کلمات کلیدی: Vertical Seismic Profile (VSP),ِDalan-Kangan formations,quality factor,Hydrocarbon reservoir
- چکیده:
- چکیده انگلیسی: An important method in oil and gas exploration is vertical seismic profile (VSP) to estimate rock properties in drilling well. Quality factor is also the crucial point of seismic attenuate in VSP data. In the present study, this factor was used to evaluate the hydrocarbon potential of Kangan Formation in one of the Persian Gulf oil fields using VSP zero offset method. Quality factor was estimated using VSP spectral ratio method. Density and porosity logs are also used to determine lithology and reservoir fluid types. Analysing Vp/Vs ratio changes is a useful tool to detect hydrocarbon accumulation. These points marked by high amplitude energy in seismograms in correlation with petrophysical logs. According to Vp/Vs plots and geological logs of the field revealed that Vp/Vs ratio is increased at depths indicating hydrocarbon presence. Data are showing good correlation to petrophysical logs. Therefore, VSP method can be applied as a suitable alternative method to find hydrocarbon reserves in those boreholes without petrophysical logs.
- انتشار مقاله: 20-05-1396
- نویسندگان: Bahman Soleimani,Kazem Rangzan,Ehsan Larki,Kurosh Shirali,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: cancer,Epigenetic,Drug resistance
- چکیده:
- چکیده انگلیسی: Cancer is caused by aberrant genetic and epigenetic changes in genes expression. DNA methylation, histone modification, and microRNAs gene deregulation are the most known epigenetic changes in different stages of cancer. Since every tumor has its own specific epigenome, any abnormal pattern is a potential biomarker for classification of different types of tumors. Despite, tumorigenesis, abnormal epigenetic changes are highly correlated with drug resistance in various stages of cancer. But, reversible nature of these abnormalities is the basis of epigenetic cancer treatment. Drugs affecting the epigenome are the new hopes in cancer treatment. The aim of this study was to investigate the role of epigenetics in tumorigenesis and also drug resistance in cancers.
- انتشار مقاله: 12-05-1395
- نویسندگان: Sorayya Ghasemi,Farnaz Razmkhah,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده: جمعیت های ویژه ای از سلول های بنیادی با قابلیت تمایز به انواع مختلف سلول، در مغز استخوان وجود دارد. به طور ایده آل سلولی که به منظور کاشت مورد استفاده قرار می گیرد، باید به راحتی به دست آمده، به سهولت بتوان آن را ذخیره کرد و دارای قابلیت زنده ماندن به مدت طولانی باشد. سلول های بنیادی مزانشیمی مغز استخوان تمام این ویژگی ها را دارا می باشند. یکی از مهمترین مزایای استفاده از سلول های بنیادی مزانشیمی مغز استخوان، امکان کاربرد این سلول ها بصورت پیوند خودی (اتولوگ) است. امروزه مطالعات بسیاری در مورد یافتن راه های ارتقا رژنراسیون آکسونی در آسیب های سیستم اعصاب مرکزی به انجام می رسد. در میان این استراتژی ها، کاشت سلول به عنوان مؤثرترین روش، پذیرفته شده است. تمایز سلول های بنیادی به انواع مختلف سلول های لاین عصبی (مانند آستروسیت ها و سلول های شبه نورونی) نقشی کلیدی در رسیدن به بهترین نتیجه در درمان آسیب های سیستم اعصاب مرکزی دارد. نمونه های مغز استخوان از استخوان ران 103 رت زنده اخذ شده و سلول های بنیادی مزانشیمی آن جدا شدند. با آنالیز RT-PCR ماهیت سلول های بنیادی مزانشیمی به اثبات رسید. القا تمایز سلول های بنیادی مزانشیمی مغز استخوان به سلول های شبه نورونی با روشی چند مرحله ای و در طی 2 هفته انجام شد. علاوه بر بررسی مورفولوژیک سلول های تمایز یافته به سمت سلول های شبه نورونی، به منظور اثبات تمایز عصبی، آزمایش ایمونوسیتوشیمی با استفاده از آنتی بادی های اولیه برای NSE به انجام رسید. همینطور آنالیز RT-PCR برای مشخص کردن بیان ژن های اختصاصی عصبی شامل NSE، MAP2، Nestin و ?-tubulin انجام گرفت. بررسی های مورفولوژیک، سلول هایی نورون شکل با زوائد بلند را نشان دادند. با استفاده از آنالیز RT-PCR و تست ایمونوهیستوشیمی، بیان ژن ها و حضور پروتئین های اختصاصی عصبی در سلول های تمایز یافته مشخص گردیدند. این مطالعه یک روش ساده و عملی را برای تمایز سلول های بنیادی مزانشیمی مغز استخوان به سلول های تمایز یافته عصبی نشان می دهد و همچنین بیان می دارد که به راحتی می توان از رت زنده برای انجام پیوندهای خودی مغز استخوان اخذ نمود.
- چکیده انگلیسی: In the bone marrow, there are certain populations of stem cell sources with the capacity to differentiate into several different types of cells. Ideally, cell transplants would be readily obtainable, easy to expand and bank, and capable of surviving for sufficient periods of time. Bone marrow mesenchymal stem cells (BM-MSCs) possess all of these characteristics. One of the most important benefits in using BM-MSCs is the possibility of autologous therapy. Recently, numerous studies have evaluated strategies that attempt to promote axonal regeneration in central nervous system (CNS) injuries. Among these strategies, cell transplantation is considered to be the most effective way. The differentiation of stem cells into different neural lineages (such as astrocytes and neural like cells) before transplantation has a critical role in achieving the best results in studies of CNS injury. In this study, BM-MSCs were isolated from bone marrow aspirates taken from the femurs of 103 live rats. The detection of BM-MSCs was performed with RT-PCR analysis, and they were then induced to differentiate into neuron-like cells in serum-withdrawal medium over a two week period using a multistep protocol. In addition to the morphological evaluation of differentiated cells, the process of neural differentiation was proven by immunocytochemical techniques using primary antibodies to Neuron Specific Enolase (NSE) to assess cell differentiation. PT-PCR analysis was performed for the evaluation of neural specific genes, which included NSE, MAP2, nestin, and ?-tubulin. Morphological evaluations detect neuron like cells with longitudinal processes. Using RT- PCR and immunocytochemistry assays, neuron specific genes and proteins following treatment of cells in serum-withdrawal induction medium was expressed. This study showed a simple and practical method for differentiating MSCs into neuron like cells, and feasibility of aspirate bone marrow from a live rat for autologous grafting.
- انتشار مقاله: 11-10-1348
- نویسندگان: Mir Sepehr Pedram,محمد مهدی دهقان,Masoud Soleimani,Davood Shafii,Seyed Marjanmehr,Zahra Nasiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Rabbit,Stem cells,defect,PVA,cartilage
- چکیده:
- چکیده انگلیسی: Objective- To evaluate the biological compatibility of differentiated stem cells embedded in poly-vinyl-alcohol (PVA) scaffolds for repair of distal femoral cartilage defect. Design- Experimental in vivo study. Animals- Twelve adult male New Zealand white rabbits were used which were divided into two groups (I, II) six rabbits each. Procedures- Mesenchymal stem cells were isolated from humerus bone marrow of group I rabbits and were cultured and differentiated on PVA scaffolds to chondrocytes. Scanning Electron Microscopy (SEM) showed well distribution of the cells inside the scaffold. A 4 mm diameter full thickness cartilage defect was created on central region of bilateral distal femoral joint surface (patellar groove) in all rabbits. In group (I) the defects were covered with autologous differentiated MSCs-seeded scaffolds; whereas the group II rabbits were left without any treatment as control ones. One month and three months after operation, three rabbits were sacrificed from each group, randomly. Histopathologic evaluation of defects was performed with H&E and trichrome staining. Results- The findings showed that in the engineered cartilage with the PVA scaffold, the defects were filled with smooth, shiny white tissue macroscopically at three months after the transplantation. Despite much connective tissue formed in defect area after three months, there was no evidence of chondrocytes in control group, whereas the defects of experimental group were almost completely filled with hyaline cartilage. Conclusion and Clinical Relevance- The results indicated there is positive possibility for partial resurfacing of cartilage defect using stem cell-seeded PVA scaffolds
- انتشار مقاله: 23-05-1392
- نویسندگان: Davood Sharifi,Pejman Mortazavi,Mohammad Mehdi Dehghan,Parviz Tajik,Mohammad Abedi,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: MicroRNAs,Hematopoietic stem cells,Umbilical cord blood,CD133,Signal transduction
- چکیده:
- چکیده انگلیسی: Background: Human umbilical cord blood (HUCB) is an acceptable and readily accessible source of stem cells. There is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated HUCB cells. The involvement of miRNAs in several biological processes has been shown. The aim of this study was to evaluate the possible changes in miRNA expression profiles in CD133+ hematopoietic cells after in vitro culture.Methods: HUCBCD133+ hematopoietic stem cells were isolated by magnetic-activated cell sorting, and then the cells were counted using flow cytometry. The cells were divided into 2 groups. In the first group, RNA was extracted and the cells of the second group were cultured in vitro for 12 days and then these cells were used to assay miRNAs expression using real-time qPCR. Results: The results showed that the expression of 349 out of 1,151 screened miRNAs was upregulated following a 12-day in vitro culture of CD133+ cells, whereas the expression of 293 miRNAs was downregulated. In addition, the expression of 509 miRNAs was not significantly altered. Another in-silico analysis involving the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to the selected miRNAs was also conducted. Conclusion: Based on our results, the in vitro expansion of HUCB resulted in altered expression levels of miRNAs. This study provides information on the effects of 2-dimensional culture of hematopoietic cells prior to transplantation for more successful transplantation.
- انتشار مقاله: 13-08-1394
- نویسندگان: Saeid Shahrabi,Saeid Kaviani,Masoud Soleimani,Ali Akbar Pourfathollah,Behnaz Bakhshandeh,Saeideh Hajizamani,Najmaldin Saki
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Adipose-Derived Mesenchymal Stromal Cells,Immunomodulatory Properties,Tumoral-MSCs
- چکیده:
- چکیده انگلیسی: Background: Differentiation, migratory properties and availability of Mesenchymal Stromal Cells (MSC) have become an important part of biomedical research. However, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells.
Objective: To characterize and compare the phenotype and cytokines of adipose derived MSCs (AD-MSCs) and tumoral-MSCs (T-MSCs) isolated from mammary tumors of BALB/c mice.
Methods: Immunophenotyping and in vitro differentiation tests were used for MSC characterization. Cytokine and enzyme profiles were assessed using ELISA and Realtime PCR, respectively. Results: T-MSCs expressed significantly higher levels of HLADR (p=0.04). Higher levels of PGE2 and COX-2 enzyme were also observed in TMSCs (p=0.07 and p=0.00, respectively). Additionally, T-MSCs expressed higher levels of iNOS and MMP9 (p=0.01 and p=0.01, respectively). T-MSCs were also able to induce higher levels of proliferation and migration of HUVEC endothelial cells in wound scratch assay compared to AD-MSCs (p=0.015).
Conclusion: Functional differences showed by the surface markers of MSCs, cytokine and enzyme production indicate the effect of different microenvironments on MSCs phenotype and function.- انتشار مقاله: 14-05-1395
- نویسندگان: Ladan Langroudi,Zuhair Muhammad Hassan,Masoud Soleimani,Seyed Mahmoud Hashemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Transplantation,Mesanchymal Stem Cells,Mixed Lymphocyte Reaction
- چکیده:
- چکیده انگلیسی: Background: Unrestricted somatic stem cells (USSC) are cord blood stem cells that have been considered as candidates for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery.
Objective: The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction (MLR) as a model for graft rejection.
Methods: USSC and mesanchymal stem cells (MSC) were isolated and cultured from cord blood and bone morrow, respectively. The immunophenotypes of USSC and MSC were evaluated by flow cytometery and USSC and MSC were co-cultured with peripheral blood lympho-cytes (PBL) in an MLR to evaluate the immunomodulatory effect of these cells as a percentage of the control response.
Results: Current study demonstrated that prolifera-tion of lymphocytes in the MLR was decreased after treatment with USSC, in a similar fashion to that seen with MSC.
Conclusion: It can be concluded that USSC have simi-lar regulatory effects as MSC on the MLR, which can be used as an indicator for poten-tial organ rejection after transplantation. Therefore, the immunregulatory effect of these cells could be used in the clinic during organ transplantation and in the management of autoimmunity.- انتشار مقاله: 16-05-1395
- نویسندگان: Hasan Ebrahimi,Masoud Soleimani,Mohammad Kazemi Arabadadi,Naser Ajmadbeigi,Gholamhossein Hassanshahi,Akbar Farjadfar,Derek Kennedy
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Differentiation,Mouse embryonic stem cell,Erythropoiesis,miR-451,miR-191
- چکیده:
- چکیده انگلیسی: Objective(s): Various microRNAs (miRNAs) are expressed during development of mammalian cells, when they aid in modulating gene expression by mediating mRNA transcript cleavage and/or regulation of translation rate. miR-191 and miR-451 have been shown to be critical regulators of hematopoiesis and have important roles in the induction of erythroid fate decision. So, the aim of this study is investigation of the miR-191 and miR-451 roles in the controlling mouse embryonic stem cell (mESC) differentiation toward the erythroid lineage.
Materials and Methods: mESCs were infected with either pCDH-miR-Off-191 viruses in pCDH-miR-Off-191 group or simultaneously with pCDH-miR-Off-191 and pCDH-miR-451 lentiviruses in simultaneous group. Then, the expression profiles of erythroid specific transcription factors and globin genes were analyzed using QRT-PCR on day 14 and 21 of differentiation. Flow cytometry analysis was used to evaluate of TER119 and CD235a erythroid specific surface markers.
Results: Gata-1, Klf-1, Epor and globin chains were found to be expressed in pCDH-miR-Off-191 and in simultaneous groups. The majority of globin chains showed changes in their expression levels with progression of differentiation from day 14 to day 21. Flow cytometry results showed that miR-451 up- regulation and miR-191 down-regulation is associated with the expression of TER119 and CD235a. Of these two groups analyzed, simultaneous group was most significantly potent in stimulation of erythroid fate decision of mESCs.
Conclusion: Together, present data demonstrate that down-regulation of miR-191 alone can enhance the differentiation of mESCs. However, the simultaneous effect of miR-451up-regulation and miR-191 down-regulation is much stronger and can have more practical use in artificial blood production.- انتشار مقاله: 05-09-1396
- نویسندگان: Gelareh Shokri,Fatemeh Kouhkan,Shahrzad Nojehdehi,Masoud Soleimani,Ali Akbar Pourfathlah,Mahin Nikoguftar Zarif,Mona Tamadon,Narges Obeidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Differentiation,Mouse embryonic stem cell,Erythropoiesis,miR-451,miR-191
- چکیده:
- چکیده انگلیسی: Objective(s): Various microRNAs (miRNAs) are expressed during development of mammalian cells, when they aid in modulating gene expression by mediating mRNA transcript cleavage and/or regulation of translation rate. miR-191 and miR-451 have been shown to be critical regulators of hematopoiesis and have important roles in the induction of erythroid fate decision. So, the aim of this study is investigation of the miR-191 and miR-451 roles in the controlling mouse embryonic stem cell (mESC) differentiation toward the erythroid lineage.
Materials and Methods: mESCs were infected with either pCDH-miR-Off-191 viruses in pCDH-miR-Off-191 group or simultaneously with pCDH-miR-Off-191 and pCDH-miR-451 lentiviruses in simultaneous group. Then, the expression profiles of erythroid specific transcription factors and globin genes were analyzed using QRT-PCR on day 14 and 21 of differentiation. Flow cytometry analysis was used to evaluate of TER119 and CD235a erythroid specific surface markers.
Results: Gata-1, Klf-1, Epor and globin chains were found to be expressed in pCDH-miR-Off-191 and in simultaneous groups. The majority of globin chains showed changes in their expression levels with progression of differentiation from day 14 to day 21. Flow cytometry results showed that miR-451 up- regulation and miR-191 down-regulation is associated with the expression of TER119 and CD235a. Of these two groups analyzed, simultaneous group was most significantly potent in stimulation of erythroid fate decision of mESCs.
Conclusion: Together, present data demonstrate that down-regulation of miR-191 alone can enhance the differentiation of mESCs. However, the simultaneous effect of miR-451up-regulation and miR-191 down-regulation is much stronger and can have more practical use in artificial blood production.- انتشار مقاله: 05-09-1396
- نویسندگان: Gelareh Shokri,Fatemeh Kouhkan,Shahrzad Nojehdehi,Masoud Soleimani,Ali Akbar Pourfathlah,Mahin Nikoguftar Zarif,Mona Tamadon,Narges Obeidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cell differentiation,Mesenchymal stromal cell,Neuron-like cells
- چکیده:
- چکیده انگلیسی: Objective(s): Umbilical cord blood-derived mesenchymal stromal cells (UCB-MSCs) are ideally suited for use in various cell-based therapies. We investigated a novel induction protocol (NIP) to improve the neuronal differentiation of human UCB-MSCs under appropriate conditions.
Materials and Methods: This experimental study was performed in Iranian Blood Transfusion Organization (IBTO), Tehran, Iran. UCB-MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator in equilibration with 5% CO2 at 37oC. For neuronal differentiation of UCB-MSCs, DMEM was removed and replaced with pre-induction medium containing RA, bFGF, EGF, and basal medium for two days. Then, NGF, IBMX, AsA, and Neurobasal medium were used for six days for this purpose. Real-time PCR was performed to analyze the neuronal differentiation of UCB-MSCs for the first time in Iran.
Results: We found that the maximum and minimum levels of gene expression were related to GFAP and nestin, respectively. In addition, our study showed that compared to other neuronal inducers, RA might play the main role in neuronal differentiation and fate of MSCs compared to other neuronal inducers.
Conclusion: Our data showed that the combination of chemical (RA, IBMX, AsA) and growth factors (NGF, EGF, bFGF) in NIP may improve the efficiency of neuronal differentiation of UCB-MSCs and may provide a new method for easy and quick application of UCB-MSCs in regenerative medicine in the future. However, the functionality of neuron-like cells must be carefully assessed in animal experiments prior to use in clinical applications.- انتشار مقاله: 10-09-1394
- نویسندگان: Hassan Rafieemehr,Maryam Kheirandish,Masoud Soleimani
- مشاهده