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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Evidence Based Care Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Inpatients,Northeast Iran,Potential food-drug interactions,Prescriptions
- چکیده:
- چکیده انگلیسی: Background: The minimization of adverse food-drug interactions will improve patient care by optimizing the therapeutic effects and maintaining proper nutritional status.
Aim: The aim of the present study was to find the main factors that may place the hospitalized patients at risk of potential food-drug interactions.
Method: This cross-sectional, descriptive study was conducted on 400 inpatients admitted to the Department of Internal Medicine of a teaching hospital in Mashhad, Northeast Iran, within 20 March 2013 to 20 April 2013. The potential food-drug interactions were evaluated for 19 commonly prescribed medications. The main factors (e.g., age, gender, education level, number of medications, and duration of the disease) that may place the patients at risk of potential food-drug interactions were analyzed for each patient.
Results: Out of the 19 commonly prescribed medications, 17 drugs (89%) were not properly used with respect to meal. Furthermore, 14 commonly prescribed drugs were found to have a high frequency (≥50%) of potential food-drug interactions. Most of the patients (n=359, 89.8%) consumed their medicines at inappropriate time with respect to meals. The results of a multiple logistic regression after adjustment for confounders revealed that the age [β=0.005, CI: 0.0-0.01; P=033], number of medications [β=0.1, CI: 0.083-0.117; P<0.001], and duration of disease [β=-0.037, CI: -0.05 to -0.023; P<0.001] were significantly associated with higher risk for potential food-drug interactions.
Implications for Practice: As the findings of the present study indicated, the number of medications was associated with the higher risk of potential food-drug interactions. Regarding this, it seems necessary to decrease the number of the prescribed medicines to lower the frequency of potential interactions among the inpatients.- انتشار مقاله: 21-04-1396
- نویسندگان: Mostafa Abdollahi,Saeid Eslami,Zhila Taherzadeh,Shayesteh Salehi,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Bone marrow,senescence,Mesenchymal Stem Cells,Growth Kinetics,Adipose tissue
- چکیده:
- چکیده انگلیسی: Objective- To investigate and compare growth potential as well as aging of mesenchymal stem cells (MSCs) derived from rat bone marrow tissue and adipose tissue (AT) occurred at epicardial and epididymal regions. Design- Experimental study. Animals- 10 Wistar Rats. Procedures- Rat MSCs occurred at bone marrow and epicardial and epididymal AT were isolated and culture expanded through several successive passages. Differentiation potential along bone, cartilage and adipose cell lineages was used to verify MSC identity of the isolated cells, and then the cells were comparatively investigated in terms of their colonogenic ability, population doubling time and growth curve characteristics. Furthermore, the number of senescent cells at different passages was quantified using senescent- associated (SA) ß galactosidase staining method. Results- MSCs from both AT appeared to have more proliferation capacity in culture than those from bone marrow since they exhibited significantly more colony number and shorter PDT value (P<0.05). Epicardial AT-MSCs indicated even more significant proliferation capacity than their epididymal counterparts. With respect to cell aging, marrow-MSCs cultures indicated higher percentages of senescent cells than AT-MSCs (P<0.05). Although the percentages of senescent cells in epididmal AT-MSCs were higher than epicardial AT- MSCs but the difference was not statistically significant. Conclusion and Clinical Relevance- Taken together we concluded that rat epicardial AT- MSCs could be appropriate cells for experimental and preclinical settings since they possess more expansion rate and less percentages of senescent cells in culture.
- انتشار مقاله: 20-05-1392
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Soura Mardpour,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Neoplasm metastasis,BRMS1,miR 31,Replacement therapy
- چکیده:
- چکیده انگلیسی: Objective(s): The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. Materials and Methods: This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells.
Results: Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently.
Conclusion: This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231.- انتشار مقاله: 27-07-1397
- نویسندگان: Samila Farokhimanesh,Mehdi Forouzandeh Moghadam,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Neoplasm metastasis,BRMS1,miR 31,Replacement therapy
- چکیده:
- چکیده انگلیسی: Objective(s): The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. Materials and Methods: This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells.
Results: Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently.
Conclusion: This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231.- انتشار مقاله: 27-07-1397
- نویسندگان: Samila Farokhimanesh,Mehdi Forouzandeh Moghadam,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: mesenchymal stem cell,Cell Proliferation,Adipose tissue,Cell aging,Cell differentiation
- چکیده:
- چکیده انگلیسی: Objective(s)
Some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. In the present study proliferation capacity and aging of such cells were explored.
Materials and Methods
Adherent cells were isolated from the collagenase digests of adipose tissues excised from rat epicardial and epididymal regions and propagated with several subcultures. The cells were then investigated whether or not they were able to differentiate into bone, cartilage and adipose cell lineages. Studied cells from two adipose tissues were also compared with respect to their in vitro proliferation capacity. The presence of senescent cells in the culture was determined and compared using senescence-associated (SA) ß-galactosidase staining method.
Results
Successful differentiations of the cells were indicative of their mesenchymal stem cells (MSCs) identity. Epicardial adipose-derived cells tended to have a short population doubling time (45±9.6 hr) than the epididymal adipose-derived stem cells (69±16 hr, P< 0.05). Colonogenic activity and the growth curve characteristics were all better in the culture of stem cells derived from epicardial compared to epididymal adipose tissue. Comparatively more percentage of senescent cells was present at the cultures derived from epididymal adipose tissue (P< 0.05).
Conclusion
Our data emphasize on the differences existed between the stem cells derived from adipose depots of different anatomical sites in terms of their proliferative capacity and in vitro aging. Such data can help understand varying results reported by different laboratories involved in adipose stem cell investigations.- انتشار مقاله: 29-06-1394
- نویسندگان: Mohamadreza Baghaban Eslaminejad,Soura Mardpour,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Evidence Based Care Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Inpatients,Northeast Iran,Potential food-drug interactions,Prescriptions
- چکیده:
- چکیده انگلیسی: Background: The minimization of adverse food-drug interactions will improve patient care by optimizing the therapeutic effects and maintaining proper nutritional status.
Aim: The aim of the present study was to find the main factors that may place the hospitalized patients at risk of potential food-drug interactions.
Method: This cross-sectional, descriptive study was conducted on 400 inpatients admitted to the Department of Internal Medicine of a teaching hospital in Mashhad, Northeast Iran, within 20 March 2013 to 20 April 2013. The potential food-drug interactions were evaluated for 19 commonly prescribed medications. The main factors (e.g., age, gender, education level, number of medications, and duration of the disease) that may place the patients at risk of potential food-drug interactions were analyzed for each patient.
Results: Out of the 19 commonly prescribed medications, 17 drugs (89%) were not properly used with respect to meal. Furthermore, 14 commonly prescribed drugs were found to have a high frequency (≥50%) of potential food-drug interactions. Most of the patients (n=359, 89.8%) consumed their medicines at inappropriate time with respect to meals. The results of a multiple logistic regression after adjustment for confounders revealed that the age [β=0.005, CI: 0.0-0.01; P=033], number of medications [β=0.1, CI: 0.083-0.117; P<0.001], and duration of disease [β=-0.037, CI: -0.05 to -0.023; P<0.001] were significantly associated with higher risk for potential food-drug interactions.
Implications for Practice: As the findings of the present study indicated, the number of medications was associated with the higher risk of potential food-drug interactions. Regarding this, it seems necessary to decrease the number of the prescribed medicines to lower the frequency of potential interactions among the inpatients.- انتشار مقاله: 21-04-1396
- نویسندگان: Mostafa Abdollahi,Saeid Eslami,Zhila Taherzadeh,Shayesteh Salehi,Marzieh Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: alternative splicing,CD8+ T Lymphocyte,D393-CD20 peptide,Burkitt lymphoma
- چکیده:
- چکیده انگلیسی: The effective discovery of clinically relevant tumor antigens holds a fundamental role for the development of
new diagnostic tools and anticancer immunotherapies. D393-CD20 mRNA is absent from normal resting B cells but
present in various malignant or transformed B cells. CD8+T lymphocytes play a central role in immunity to cancer.
In this study, we want use from T CD8+ against D393-CD20 for effect in RAMOS cell line. After isolation and
expanding of specific TCD8 + Lymphocyte against D393-CD20 antigen, for examining the effect of specialized T
lymphocyte clone of D393-CD20 antigen on RAMOS cell line, we co-cultured them together, and the rate of apoptosis
were examined by flow cytometry and cytotoxicity techniques by using MTT technique. We observed that specialized
TCD8+ lymphocyte of D393-CD20 antigen can induce apoptosis in malignant B-lymphocytes, and this antigen can
be a proper target for immunotherapy.- انتشار مقاله: 19-07-1397
- نویسندگان: Hamid Chegni,Zuhair M Hassan,Roberto Nisini,Marzieh Ebrahimi,Farzaneh Sabouni
- مشاهده