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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Alzheimer,Hippocampus,Spatial Memory,Ziziphora clinopodioides
- چکیده:
- چکیده انگلیسی: Objective: Alzheimer's disease is a neurodegenerative disorder associated with gradual loss of cognitive and memory abilities. It was shown that the hippocampus is one of the first structures in the brain that is affected by the disease. Ziziphora clinopodioides (Z. clinopodioides) is a member of Lamiaceae family and contains various substances.
Materials and Methods: In this experimental study, 72 adult male Wistar rats were used for behavioral and histopathologic studies. They were divided into nine groups included: control, negative control (Alzheimer), positive control (Alzheimer's treated with rivastigmine), aCSF (artificial cerebrospinal fluid) + ziziphora extract with doses of 200,400, and 600 mg/kg, and STZ (stereptozotocine)+ziziphoraextract in 200,400,600 mg/kg doses. The injury was created with bilaterally intraventricular injection. The spatial memory was studied by passive avoidance test and neuronal density was evaluated by dissector method. To examine the histopathological lesions, Congo red and toluidine blue staining were done. Data were analyzed by ANOVA Minitab software.
Results: The memory index (neuronal density and passive avoidance test results) showed a significant decrease in negative control group compared to control (p≤0.001). Treatment with the hydroalcoholic extract at the doses of 400 and 600 mg/kg showed a significant increase in memory index in rats with Alzheimer's disease (p≤0.001). The effect of 200 mg/kg extract was not significantly different from that of the negative control group. The results of histological analysis indicated beta-amyloid plaques formation in the control group as compared to the negative control group while treatment with the extract at the doses of 400 and 600 mg/kg, significantly reduced beta-amyloid plaques formation.
Conclusion: These findings suggest that the extract of Z. clinopodioides can improve Alzheimer's condition and alleviate memory and histopathologic damages; also, it decreases beta-amyloid plaques and apoptosis in CA1 region of the hippocampus.- انتشار مقاله: 13-05-1397
- نویسندگان: Samaneh Sedighi,Maryam Tehranipour,Gholamhassan Vaezi,Vida Hojati,Hamid Hashemi-Moghaddam
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Ethylene glycol,Renal calculi,Cichorium intybus L,Flower
- چکیده:
- چکیده انگلیسی: Objective: Urolithiasis remains a global problem. Despite the availability of numerous methods, no definite therapeutic agent has been yet introduced for the prevention or treatment of kidney stones. In this study, we evaluated the possible preventive effects of aqueous extract of Cichorium intybus L. (chicory) flowers on ethylene glycol-induced renal calculi in rats.
Materials and Methods: A total of 24 Wistar rats were randomly divided into four groups and were treated for 30 days. Group A received drinking tap water, while groups B, C, and D were administered with 1% ethylene glycol for induction of calcium oxalate stone formation. Rats in groups C and D received intraperitoneal injections of the aqueous extract of chicory flowers (50 and 200 mg/kg, respectively) since the first day of the experiment. The urine volume, urine pH, and urinary levels of oxalate, citrate, calcium, uric acid, and creatinine as well as serum levels of calcium, uric acid, and creatinine were measured. After 30 days, the rats' kidneys were removed and prepared for histological evaluation of calcium oxalate deposits. One-way analysis of variance (ANOVA), followed by Tukey's test, was performed, using SPSS version 20.
Results: The number of calcium oxalate crystals was significantly higher in group B (ethylene glycol-only treated animals), compared to group A (control), group C (50 mg/kg of aqueous extract), and group D (200 mg/kg of aqueous extract) (p<0.05). On day 30, the urine level of citrate, oxalate (p>0.05), and creatinine (p<0.05), as well as urine pH (p<0.05) decreased in groups C and D, compared to group B. Also, urine calcium level, urine uric acid (p>0.05), and urine volume (p<0.05) were higher in group D, compared to group B. In addition, the serum level of calcium, creatinine (p<0.05), and uric acid (p<0.001) decreased in groups C and D.
Conclusion: The aqueous extract of chicory flower (50 mg/kg) could reduce the number of calcium oxalate deposits in the urine and reduce the level of serum parameters.- انتشار مقاله: 23-11-1395
- نویسندگان: Mahdieh Emamiyan,Gholamhassan Vaezi,Maryam Tehranipour,Khdije Shahrohkabadi,Abdolhossein Shiravi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Achillea biebersteinii,Sciatic nerve,Degeneration,Neuroprotective
- چکیده:
- چکیده انگلیسی: Background and aims: Neurons are injured under physical, chemical and pathological conditions. The effect of peripheral nervous system injuries may return as retrograde to the cell body of neurons in central nervous system resulting in brain and spinal cord degeneration. Because of high content of camphor and borneol, Achillea biebersteinii from Asteracea family may be effective in prevention the nervous injury progress. So, the aim of the present study was to evaluate the neuroprotective effect of Achillea biebersteinii leaves extracts on spinal motoneurons denegation after sciatic nerve compression in rats. Methods: This study was carried out on forty eight male Wistar rats divided in eight groups including control (A), compression (B), compression and treated with 50 (C), 75 (D), 100 (E) mg/kg of alcoholic extract, compression and treated with 50 (F), 75 (G), 100 (H) mg/kg of aqueous extract of Achillea biebersteinii. In compression and experimental groups right sciatic nerve was highly compressed for 60 s. Extract was injected intraperitoneally in the first and second week after compression. After four weeks post-operative the lumbar segments of spinal cord were sampled, processed, sectioned and stained with toluidine blue (pH 4.65). Using stereological quantitative technique, the number of alpha motoneurons in the right horn of spinal cord were counted and compared with each other. Results: According to the results, neuronal density showed significant decrease in compression group compared to control group (P= 0.000). Neuronal density in experimental groups C, D and E (50, 75, 100 mg/kg of alcoholic extract) as well as F, G and H (50, 75, 100 mg/kg of aqueous extract) was significantly (P< 0.001) more than that of compression group. Data analysis also showed that for both alcoholic and aqueous extract the concentration of 75 mg/kg had stronger activity than concentrations of 50 and 100 mg/kg. Conclusion: The results showed that alcoholic and aqueous extracts of Achillea biebersteinii leaves have neuroprotective effects on spinal cord alpha motoneurons after injury.
- انتشار مقاله: 14-04-1393
- نویسندگان: Mahbobe Alikhanzade,Maryam Tehranipour,Jina Khayatzade
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Achillea biebersteinii,Sciatic nerve,Degeneration,Neuroprotective
- چکیده:
- چکیده انگلیسی: Background and aims: Neurons are injured under physical, chemical and pathological conditions. The effect of peripheral nervous system injuries may return as retrograde to the cell body of neurons in central nervous system resulting in brain and spinal cord degeneration. Because of high content of camphor and borneol, Achillea biebersteinii from Asteracea family may be effective in prevention the nervous injury progress. So, the aim of the present study was to evaluate the neuroprotective effect of Achillea biebersteinii leaves extracts on spinal motoneurons denegation after sciatic nerve compression in rats. Methods: This study was carried out on forty eight male Wistar rats divided in eight groups including control (A), compression (B), compression and treated with 50 (C), 75 (D), 100 (E) mg/kg of alcoholic extract, compression and treated with 50 (F), 75 (G), 100 (H) mg/kg of aqueous extract of Achillea biebersteinii. In compression and experimental groups right sciatic nerve was highly compressed for 60 s. Extract was injected intraperitoneally in the first and second week after compression. After four weeks post-operative the lumbar segments of spinal cord were sampled, processed, sectioned and stained with toluidine blue (pH 4.65). Using stereological quantitative technique, the number of alpha motoneurons in the right horn of spinal cord were counted and compared with each other. Results: According to the results, neuronal density showed significant decrease in compression group compared to control group (P= 0.000). Neuronal density in experimental groups C, D and E (50, 75, 100 mg/kg of alcoholic extract) as well as F, G and H (50, 75, 100 mg/kg of aqueous extract) was significantly (P< 0.001) more than that of compression group. Data analysis also showed that for both alcoholic and aqueous extract the concentration of 75 mg/kg had stronger activity than concentrations of 50 and 100 mg/kg. Conclusion: The results showed that alcoholic and aqueous extracts of Achillea biebersteinii leaves have neuroprotective effects on spinal cord alpha motoneurons after injury.
- انتشار مقاله: 14-04-1393
- نویسندگان: Mahbobe Alikhanzade,Maryam Tehranipour,Jina Khayatzade
- مشاهده