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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Lung cancer,Verapamil,Chemoresistance Chemotherapy,H1299 cells
- چکیده:
- چکیده انگلیسی: Objective(s): Chemoresistance remains the main causes of treatment failure and mortality in cancer patients. There is an urgent need to investigate novel approaches to improve current therapeutic modalities and increase cancer patients' survival. Induction of drug efflux due to overexpression of P-glycoproteins is considered as an important leading cause of multidrug resistance. In this study, we investigated the role of combination treatments of docetaxel and vinblastine in overcoming P-glycoprotein mediated inhibition of apoptosis and induction of cell proliferation in human non-small cell lung carcinoma cells. Materials and Methods:Cell proliferation and apoptosis were assessed using MTT assay and DAPI staining, respectively. P-glycoprotein expression was evaluated in gene and protein levels by Real-time RT-PCR and Western blot analysis, respectively. Results: Combination treatment of the cells with docetaxel and vinblastine decreased the IC50 values for docetaxel from (30±3.1) to (15±2.6) nM and for vinblastine from (30±5.9) to (5±5.6) nM (P≤0.05). P-glycoprotein mRNA expression level showed a significant up-regulation in the cells incubated with each drug alone (P≤0.001). Incubation of the cells with combined concentrations of both agents neutralized P-glycoprotein overexpression (P≤0.05). Adding verapamil, a P-glycoprotein inhibitor caused a further increase in the percentage of apoptotic cells when the cells were treated with both agents. Conclusion:Our results suggest that combination therapy along with P-glycoprotein inhibition can be considered as a novel approach to improve the efficacy of chemotherapeutics in cancer patients with high P-glycoprotein expression.
- انتشار مقاله: 27-12-1394
- نویسندگان: Mahsa Mohseni,Nasser Samadi,Parisa Ghanbari,Bahman Yousefi,Maryam Tabasinezhad,Simin Sharifi,Hossein Nazemiyeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Proliferation,Invasion,survivin ,migration,S1P
- چکیده:
- چکیده انگلیسی: Objective(s):Degradation of sphingosine 1-phosphate (S1P), as a bioactive lipid, or deregulation of its production involves in tumor progression, metastasis and chemoresistance. Since the tumor progression effects of S1P and its mechanism in chronic lymphoblastic leukemia and non-small cell lung cancer is not fully understood, we investigated the role and one of the mechanisms of S1P in tumor progression of SKW3 and H1299 cells. Materials and Methods: The effects of S1P on proliferation, invasion and migration was studied using MTT assay, soft-agar colony forming assay and trans-well migration assay, respectively. In order to find out the mechanisms of S1P action, the role of S1P on expression of Survivin gene was assessed by real-time RT-PCR. Results:Our results demonstrated that although invasion was shown only in H1299 cells, low concentration of S1P, especially at 1 μM, mediated proliferation and migration in both cell lines. In addition, these effects of S1P in tumor progression are S1P receptor-dependent, and Survivin plays a key role in S1P tumorigenesis. Conclusion:Our results confirmed the involvement of S1P and its receptors in tumor progression of SKW3 and H1299. We also investigated another mechanism of S1P involved in cell survival, tumor progression, and Survivin signaling. In conclusion, data demonstrated the importance of this molecule as a target for designing new anticancer drugs such as anti-S1P monoclonal antibody for inhibiting major downstream signaling, which plays significant role in tumorigenesis.
- انتشار مقاله: 03-06-1394
- نویسندگان: Maryam Tabasinezhad,Hamid Ghaedi,Parisa Qanbari,Mahsa Mohseni,Mehdi Sabzichi,Nasser Samadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: JC Virus,colorectal adenocarcinoma,Polymerase Chine Reaction
- چکیده:
- چکیده انگلیسی: Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous
Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have
been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and
rectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancer
type Adenocarcinoma. Material and Methods: A total of 120 formalin-fixed paraffin-embedded tissue blocks samples
were collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50%
males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR was
carried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR products
of 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determine
homology identity with other JCV. Results: 4/40(10%) samples of test group and 10/80 (12.5%) of control samples
were positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female
(P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group (0%) (p= 0.29). Conclusion: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors
(p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCV
DNA was among 10% cases of age group >50 years and 0% of age group protein expression might explain the increased risk of colorectal cancer and requires further investigation.- انتشار مقاله: 08-07-1397
- نویسندگان: Azadeh Haghi Navand,Ali Teimoori,Manoochehr Makvandi,Nilofar Nisi,Seyed Saeid Seyedian,Nastaran Ranjbari,Kambiz Ahmadi Angali,Hadis Keyani,Maryam Tabasi,Keyvan Pourjabari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Phylogenetic analysis,Colorectal cancer (CRC),Human bocavirus (HBoV)
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) as a worldwide human health concern is identified being a multifactorial
subject that infection with specific viral particles such as oncogenic viruses is research interest. Human bocavirus (HBoV)
as a recent isolated virus has been investigated in many respiratory and enteric diseases but rare studies evaluates it in
tissue specimens especially in cancerous sections. The aim of this study was to detect the presence of HBoV genome
and its genotyping in CRC patient’s tissue and compare the result with matched healthy control group tissue. Method:
in this retrospective case-control study, CRC cases were sporadic and non-familial cancerous while control subjects had
healthy or non-malignant lesions in colon tissue. A conventional-PCR performed by specific primers for HBoV VP1
gene. After sequencing of positive PCR products, raw data used for trimming and alignment by bioinformatics software
CLC Main Workbench 5 and MEGA5. SPSS v.22 used for statistical calculations. Result: a total of 157 subjects were
participated that 66 were diagnosed as CRC cases and 91 were non-CRC colon tissue as control group that matched by
the cases. The mean age (y) ± standard deviation of each case and control groups were 59.35±14.48 and 57.21±14.66,
respectively. PCR results showed there were 1.3% (2/157) HBoV positive (of each groups one was positive). Sequencing
analysis showed all were HBoV-1 genotype. Conclusion: our study showed there are low rate of HBoV genome in
Iranian CRC and non-CRC colon tissue. Furthermore, the predominant genotype in our studied subsets were HBoV-1
according to phylogenetic analysis.- انتشار مقاله: 01-05-1397
- نویسندگان: Mohammad Hadi Karbalaie Niya,Hossein Ajdarkosh,Fahimeh Safarnezhad Tameshkel,Mahshid Panahi,Maryam Tabasi,Behnaz Bouzari,Mahdi Alemrajabi,Hossein Keyvani
- مشاهده