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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Acute myeloid leukemia, Acute promyelocytic leukemia, Blood cancer, Survival, t(15,17)(q22,q12)
- چکیده:
- چکیده انگلیسی: Background & Objective: Acute promyelocytic leukemia (APL) with t(15;17)(q22;q12) is a relatively common subtype of acute myeloid leukemia (AML). Here, our objective was to ascertain the survival of patients with this leukemia in north-east of Iran.
Methods: Survival rates of 42 APL patients with t(15;17)(q22;q12) were assessed. Clinical information was obtained from archived medical records. Statistical analysis was performed by SPSS 18 software using log-ranked test and Kaplan Maier survival analysis.
Results: Females and males comprised 49% and 51%, respectively. The mean age at diagnosis was 34.3 ± 14.1 years old. During the study period, 17 demises occurred in males, while this number was 7 in females. The mean survival of patients (month) was 23.22 ± 3.57 (95% CI: 16.21 ± 30.2). The five-year survival rate obtained 30%. Regarding demographic and clinical features, the highest rates of 5-year survival were recorded in patients with 20-35 years old (47.6%), males (51%), white blood cell count <10±/l (48%), and platelet count >140 ± /l (100%).
Conclusion: Younger age, lower WBC count and higher platelet count were significantly associated with longer survival in AML patients with t(15;17)(q22; q12).- انتشار مقاله: 07-11-1397
- نویسندگان: Hossein Ayatollahi,Ali Bazi,Mohammad Hadi Sadeghian,Ali Fani,Payam Siyadat,Maryam Sheikhi,Omolbanin Sargazi-aval
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: MPL (W515K/L),JAK2 (V617F),Myeloproliferative neoplasm
- چکیده:
- چکیده انگلیسی: Background and Objective: Janus kinase 2 (JAK2) and Myeloproliferative Leukemia (MPL) mutations are confirmatory indicators for Myeloproliferative Neoplasm (MPN). The current study was performed to determine the frequency of MPL mutation in MPN patients without JAK2 mutation, in order to assign MPL mutation frequency in North-East of Iran.
Methods: Total of 105 negative JAK2 cases including 5 Myeloproliferative Disorders (MPD), 15 Polycytemia Vera (PV) and 15 Essential Thrombocytosis (ET) who referred to Qaem Medical Center were assigned to this study. ARMS-PCR was carried out for measuring MPL mutations.
Results: A significant difference was observed between MPL mutant and non-mutant groups from overview of MPL mutation (P=0.00001). From the total studied population, 14.28% were ET cases and 4.71% of them had splenomegaly. About 66.66% had thrombocytosis and 33.33% of all the individuals had leukocytosis according to WHO criteria, and 4.76% of non-MPL mutant individuals had splenomegaly (P=1).
This mutation was reported in 4-6% of ET and PMF individuals. In this research, 4.76 % of studied individuals had MPL (W515L/K) mutation, which were diagnosed with ET.
Conclusion: Generally, the presence of JAK2 and MPL mutations are the most important criteria for MPN diagnosis. The obtained frequency of MPL mutation was similar to previous studies. Despite the high frequency of JAK2 and Philadelphia abnormality, MPL mutation was rare in myeloprolifrative disorders. Further studies are suggested to investigate its prognostic effects for these diseases.- انتشار مقاله: 26-01-1396
- نویسندگان: Seyyede Fatemeh Shams,Hossein Ayatollahi,Mohammad Hadi Sadeghian,Monavar Afzal Aghaee,Sepideh Shakeri,Ehsan Yazdandoust,Maryam Sheikhi,Nafiseh Amini,Samane Bakhshi,Afsane Bahrami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Gleason score,Prostate adenocarcinoma,BRAF mutation
- چکیده:
- چکیده انگلیسی: Background and Objective: BRAF mutations were studied in various populations for prostate carcinoma (PC); however, mutations in BRAF gene are unusual compared to KRAS. Oncogenic activating of BRAF mutations were studied lately in almost 0%-10% of prostate cancer cases.
Methods: In this retrospective study, we gathered 100 formalin-fixed paraffin-embedded samples of prostate adenocarcinoma. A hundred archived samples of adjacent benign prostatic hyperplasia were chosen as normal control. This study was done in pathology laboratory of Qaem Hospital during 2013-2015.
Results: Total number of 200 PC and normal cases was investigated for BRAF V600E mutation. The BRAF V600E mutation was found in only 4 patients but it was not detected in normal cases. There were no significant differences between patient and control groups for this mutation (P>0.99). The frequency of BRAF V600E mutation was not significant in different age groups (P>0.285); the most frequency was related to the age range of 71-80. No significant difference was observed between tumor grade and BRAF mutation (P=0.21).
Conclusion: According to our findings, BRAF gene mutations did not play essential role in PC. Therefore, anti-BRAF (V600E) could not be considered as a proper target for therapy.- انتشار مقاله: 20-02-1396
- نویسندگان: Amir Hossein Jafarian,Khatoone Mirshekar,Sare Etemad,Masoumeh Jafaripour,Mansoore Darijani,Maryam Sheikhi,Hossein Ayatollahi,Sepideh Shakeri,Seyyede Fatemeh Shams,Saeed Davari
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Quantitative real-time PCR,fluorescent in situ hybridization,HER-2/neu gene quantification
- چکیده:
- چکیده انگلیسی: Background:Breast cancer remains the most common and second lethal cancer in females. HER-2/neu is one of the most important amplified oncogene in breast cancer. The amplification of HER-2 is correlated with decreased survival, metastasis, and early recurrence. The amplification of HER-2/neu gene and synthesis of the protein are reported in 10%-34% of breast cancer cases associated with tumor size, advanced tumor stage, high-grade tumor, young age at diagnosis, absence of steroid hormone receptor, and lymph node involvement.
Methods: Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) methods are options to evaluate HER-2 expression. The current study aimed at identifying the correlation between FISH and real-time polymerase chain reaction (PCR) in measuring HER-2 expression.
Results: The study investigated the performance of the real-time PCR as measured against FISH method in IHC +2 borderline cases. In a total of 120 IHC 2+ samples, 58.3% were negative and 41.6% positive for HER-2 gene, confirmed by FISH as a gold standard method. The real-time PCR ratio was HER-2 gene by FISH as a gold standard assay.
Conclusion: Despite the fact that real-time PCR is a promising method to evaluate HER-2 over expression and a supplementary array to FISH, according to the results of the present study it cannot be utilized instead of gold standard techniques; therefore, additional studies should be carried out to appraise the value of this method to evaluate HER-2 over expression.- انتشار مقاله: 06-12-1394
- نویسندگان: Fatemeh Homaei Shandiz,Azar Fani,Sepideh Shakeri,Maryam Sheikhi,Abouzar Ramezani Farkhani,Arezoo Shajiei,Hossein Ayatollahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Helicobacter pylori,Sequencing,Gastric cancer,KRAS,Mutation
- چکیده:
- چکیده انگلیسی: Objective(s): KRAS proto-oncogene mutation can be considered a diagnostic factor for treating various malignancies. Helicobacter pylori infection, a risk factor for stomach cancer, may cause DNA damage and genetic changes. The aim of the current study was to assess the association of gastric cancer and KRAS mutation, demographic factors, and H. pylori infection.
Materials and Methods: DNA was extracted from a total of 140 FFPE gastric cancer tissue samples. detection of KRAS mutation (codons 12 and 13) in tumors was performed by PCR amplification, followed by gel electrophoresis and DNA sequencing. PCR diagnosed any H. pylori infection.
Results: KRAS mutation was detected in 6 of the 140 (4.2%) gastric cancer tissue samples. 18 samples (12.8%), all of which were male (P<0.05), tested positive for H. pylori infection. KRAS mutations were present in 22.2% (4/18) of the samples with H. pylori infection (P<0.05). The mean age of patients was 62.25±12.61 years (range: 30–93 years). A male predominance (2.5 to 1) was reported in the gastric cancers, and at diagnosis, women were significantly younger than men (P=0.004). No association was observed between age or gender and KRAS mutation. Neither was one found between age and H. pylori infection. Tumors from H. pylori+ subjects were significantly more likely to have KRAS mutation than tumors from H. pylori- subjects (OR=17.1).
Conclusion: The data suggest that H. pylori infection when compared with the absence of H. pylori infection, is associated with a higher prevalence of KRAS mutation in gastric cancer.- انتشار مقاله: 31-02-1397
- نویسندگان: Raheleh Jabini,Seyed Ahmad Eghbali,Hosein Ayatolahi,Maryam Sheikhi,Mohammadreza Farzanehfar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Helicobacter pylori,Sequencing,Gastric cancer,KRAS,Mutation
- چکیده:
- چکیده انگلیسی: Objective(s): KRAS proto-oncogene mutation can be considered a diagnostic factor for treating various malignancies. Helicobacter pylori infection, a risk factor for stomach cancer, may cause DNA damage and genetic changes. The aim of the current study was to assess the association of gastric cancer and KRAS mutation, demographic factors, and H. pylori infection.
Materials and Methods: DNA was extracted from a total of 140 FFPE gastric cancer tissue samples. detection of KRAS mutation (codons 12 and 13) in tumors was performed by PCR amplification, followed by gel electrophoresis and DNA sequencing. PCR diagnosed any H. pylori infection.
Results: KRAS mutation was detected in 6 of the 140 (4.2%) gastric cancer tissue samples. 18 samples (12.8%), all of which were male (P<0.05), tested positive for H. pylori infection. KRAS mutations were present in 22.2% (4/18) of the samples with H. pylori infection (P<0.05). The mean age of patients was 62.25±12.61 years (range: 30–93 years). A male predominance (2.5 to 1) was reported in the gastric cancers, and at diagnosis, women were significantly younger than men (P=0.004). No association was observed between age or gender and KRAS mutation. Neither was one found between age and H. pylori infection. Tumors from H. pylori+ subjects were significantly more likely to have KRAS mutation than tumors from H. pylori- subjects (OR=17.1).
Conclusion: The data suggest that H. pylori infection when compared with the absence of H. pylori infection, is associated with a higher prevalence of KRAS mutation in gastric cancer.- انتشار مقاله: 31-02-1397
- نویسندگان: Raheleh Jabini,Seyed Ahmad Eghbali,Hosein Ayatolahi,Maryam Sheikhi,Mohammadreza Farzanehfar
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Sulfur mustard,Leukemia,FLT3-TKD835 mutation
- چکیده:
- چکیده انگلیسی: Objective(s):Sulfur mustard (SM) was used by the Iraqi army against the Iranian troops in the Iran-Iraq war from 1983–1988. This chemical gas affects different organs including the skin, lungs and the hematopoietic system. Any exposure to SM increases the risk of chromosomal breaking, hyperdiploidy and hypodiploidy. Studies have shown that the risk for acute myeloblastic and lymphoblastic leukemia increases in veterans exposed to SM. FLT3 mutations including ITD and TKD mutations had been observed in some cases of leukemia. Therefore, we aimed to investigate the frequency of FLT3-TKD835 mutations in the veterans exposed to SM agent.
Materials and Methods: We studied 42 patients who were exposed to SM during the war in Khorasan Razavi province, Mashhad, Iran in 2012. As control group, 30 healthy males were selected from first-degree relatives of the patients. For assessment of TKD835 mutation, DNA was extracted and RFLP-PCR was performed.
Results: Analysis of RFLP-PCR data showed no FLT-3 TKD mutation in any of the patients.
Conclusion: Although contact with SM can increase the risk of malignancy especially hematologic neoplasms, results of the study show that another mechanism of leukemogenesis, other than FLT3-TKD mutation, may be the reason for increased risk of leukemia in SM toxicity.- انتشار مقاله: 13-07-1394
- نویسندگان: Hossein Ayatollahi,Mohammad Rafiee,Mohammad-Reza Keramati,Mahdi Balali-Mood,Ali Asgharzadeh,Mohammad Hadi Sadeghian,Maryam Sheikhi,Nafiseh Amini,Azam Moradi Zarmehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: hookah,micronucleus,Repair index,buccal mucosa
- چکیده:
- چکیده انگلیسی: Background and aim: Cigarettes, hookah, and tobacco are the most important etiologic factors for oral cancers
and dysplastic lesions. This study was undertaken to determine the correlation between hookah use and the percentage
of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa; and secondly to compare
hookah user and non-user in terms of repair index. Materials and methods: The present historical cohort study was
carried out on 72 samples taken from 36 hookah users and 36 control subjects. Smear samples were obtained from
participants’ buccal mucosa for cytological evaluation using Papanicolaou technique. Then, the percentages of cells
with micronucleus, karyorrhexis, karyolysis, and broken egg were recorded and the repair index was calculated. Data
were analyzed using Mann-Whitney U test. Results: A total of 72 samples taken from 36 hookah users and 36 control
subjects were evaluated. The means of micronucleus scores in the buccal mucosa cells of hookah users and controls
were 10.7±2.6 and 5.8±2.0, the karyorrhexis scores in the hookah users and controls were 0.1±0.06 and 0.04±0.06,
and the karyolysis scores in hookah users and controls were 0.16±0.05 and 0.08±0.06, respectively. These differences
were statistically significant between hookah users and controls (P<0.001). The broken egg score was 0.66±0.07 for the
hookah users and 0.03±0.04 for the control group, revealing a statistically significant difference (P<0.036). Finally, the
repair index values were 0.03±0.01 and 0.05±0.13 in hookah users and controls, respectively. This difference was also
significant (P<0.026). Conclusion: The percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken
egg in the buccal mucosa of hookah users were significantly higher than those in control group; in addition, the repair
index of the buccal mucosa cells in hookah users was significantly lower than that in the control group.- انتشار مقاله: 31-05-1397
- نویسندگان: Mehrdad Taghibakhsh,Sareh Farhadi,Afsaneh Babaee,Maryam Sheikhi
- مشاهده