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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Macrophage,Shark,Liver Oil
- چکیده:
- چکیده انگلیسی: Objective(s) Shark Liver Oil (SLO) is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency. Materials and Methods Peritoneal macrophages were separated and cultured (3*105/well). At first, the effect of killed-Candida (200 cells/well) on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO. Results Killed-Candida suppressed the ability of MTT reduction and hence macrophages viability (P=0.026), but addition of SLO (100 mg/ml) significantly enhanced cell viability (P=0.00). So, SLO could neutralize the inhibitory effect of Candida. Conclusion Simultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement.
- انتشار مقاله: 11-07-1394
- نویسندگان: Monire Hajimoradi,Saeed Daneshmandi,Zuhair Mohammad Hassan,Maryam Roudbary
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Nitric oxide,Macrophages
- چکیده:
- چکیده انگلیسی: Objective(s) The fractions of Candida albicans have been used as an immunomodulator. The present work assessed the effect of different fractions of C. albicans on nitric oxide (NO) production by mice peritoneal macrophages. Materials and Methods Cell wall and cytoplasmic fractions of C. albicans ATCC 10321 strain were extracted. Mice peritoneal macrophages were purified and cultured. Different concentrations of both fractions and also killed C. albicans cells were used for macrophages stimulation and evaluation of NO production. NO amount was detected in culture supernatants of macrophages by Griess reagent. Also, MTT assay was performed to assess the viability of macrophages. Results The results elucidated that suppressive effect of cell wall proteins on NO release was significant at the dose of 100 pg/ml (P=0.01), while cytoplasmic fraction increased NO amount at the dose of 1 pg/ml compared to the control group (P=0.003). Augmentation of NO production was statistically significant at 200 killed C. albicans per well (P=0.006). Conclusion According to our findings, cytoplasmic fractions and killed C. albicans have a positive effect on NO production by peritoneal macrophages, while cell wall fractions did not. Therefore, it is proposed that C. albicans fractions can be studied more as inflammation modulators.
- انتشار مقاله: 08-07-1394
- نویسندگان: Monire Hajimoradi,Saeed Daneshmandi,Maryam Roudbary,Shahla Roudbar Mohammadi,Zuhair Mohammad Hassan,Rozita Heidari,Hasan Namdar Ahmadabad
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,RT-PCR,vaginal Candidiasis,Biofilm formation,EFG1 gene
- چکیده:
- چکیده انگلیسی: Candida albicans has the ability to change between yeast and hyphal cells and is known to be a virulence property. Efg1gene of C.albicans is as a main transcription factor that plays pivotal roles in biofilm formation The aim of the current study is to investigate the presence of Efg1 gene in Candida albicans isolates from women with vaginal candidiasis and its impact on biofilm formation.
We used 50clinical isolates subjected to PCR-RFLP to confirm C.albicans. Total RNA was extracted from C. albicans isolates by glass bead and lysis buffer, and cDNA was synthesized using Reverse Transcriptase enzyme. RT-PCR was used to evaluate the expression of Efg1 gene. Biofilm formation was evaluated in 96-well microplate and then tetrazolium reduction was assayed. All data were analyzed using t-test .
50 clinical isolates out of 150 were confirmed as C.albicans by using PCR-RFLP. 47(94%) isolates out of 50 present Efg1 gene by using PCR, and 12(25.53%) isolates out of 47 expressed Efg1 gene by the RT-PCR. Isolates which have Efg1gene successfully form dense biofilm on microplate. The results of the tetrazolium reduction assay test showed that 2 isolates with Efg1 gene expression and 2 isolates lacked Efg1 gene expression which was significant at P-value of0.014 (P<0.05).
Results of MTT assay identified biofilm formation in isolates with Efg1gene expression and no biofilm formation in isolates which no Efg1 gene expression.It could then be concluded that the growth of hyphae is influenced by environmental factors which is also essential for biofilm formation.- انتشار مقاله: 13-05-1394
- نویسندگان: Melody Salahshour,Maryam Roudbary,Kyomars Amini
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,hwp1,vaginal Candidiasis,adherence
- چکیده:
- چکیده انگلیسی: Candida albicans is an opportunistic fungal pathogen found as mycoflora in the human body surfaces. Sevral genes play a crucial rule in its virulence including Hwp1 (hyphal wall protein 1), BCR1 and ALS gene family. Hwp1 gene is a responsible for coding a cell surface protein, which mediates biofilm formation in candida albicans. Here we investigated the presence of the HWP1gene was characterised among Candida albicans isolates in women with recurrent vaginal canididasis and further we studies its role in cell adherence. We used 50 Candida albicans clinical isolates resistant to Fluconazole. RNA (of samples were extracted using glass bead and lysis buffer and cDNA was synthesized using reverse transcriptase enzyme. Expression of (HWP1) gene was analysed using reverse transcriptase-plolymerase chain reaction (RT-PCR). The ability of adherence of the isolates with or without the expression of HWP1 were characterized using Hela cells. Statistical analysis were performed using t-test and two-way ANOVA SPSS software. Our results showed that the HWP1 gene were present in 47 samples (94%) out of 50 isolates, 27 samples (57%) had expression of HWP1. The result of adherence assay revealed that the isolates with the expression of HWP1 gene and control isolates was statistically different (p<0.05). In conclusion, the isolates with the expression of HWP1 gene has the higher ability to adhere the epithelial mucosal cell surface. Our data support a positive correlation between the expression of HWP1 gene and the ability of adherence to epithelial cells.
- انتشار مقاله: 27-03-1392
- نویسندگان: Maryam Hosseini Por,Shahla Roudbarmohammadi,Maryam Roudbary,Bita Bakhshi,Zahra Farhadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: vaginal Candidiasis,Candida albicans, ALS1,ALS3
- چکیده:
- چکیده انگلیسی: Candida albicans is an opportunistic fungi that is able to thrive in many host niches, including the skin, mucosal, surfaces, the blood stream and internal organs. Agglutinin-like sequence (ALS) genes which could play a role in forming biofilms, adherence to host surfaces as a virulence factor and antifungal drug resistant. The purpose of the present study was to evaluate the presence ALS1 and ALS3 genes in clinical strains of C.albicans isolated from women with vaginal candidiasis symptom. Vaginal swabs were collected from patients suffering from vaginal candidiasis and admitted to health care centers (Tehran, Iran) from June 2011 until June 2012 and cultured on Sabaouraud Dextros Agar for 48 hours. A PCR-RFLP was used with MspI restriction enzyme for identification of Candida albicans. Susceptibility testing of 53 clinical isolates of C.albicans isolates was done against Fluconazole by using disk diffusion method. Total DNA was extracted from C.albicans isolates and PCR assay was used to evaluate the presence of ALS1, ALS3 and internal control (ACT1) genes. 53 clinical isolates out of 100 were identified as C.albicans by using PCR-RFLP. Three (5.7%) of them were susceptible and 50 (94.3%) were resistant to fluconazole. 44 patients (83%) with vaginal candidiasis were positive for ALS1 gene and 48 ones (90.5%) were positive for ALS3. According our finding, a significant correlation was seen between the presence of ALS1 and ALS3 genes and fluconazole resistance in C.albicans isolates.
- انتشار مقاله: 01-03-1391
- نویسندگان: Maryam Roudbary,Shahla Roudbarmohammadi,Bita Bakhshi,Zahra Farhadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Current Medical Mycology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Expression,Vulvovaginal candidiasis,Filamentous growth,TUP1 gene
- چکیده:
- چکیده انگلیسی: Background and Purpose: Recurrent vulvovaginal candidiasis (RVVC) is one of the most common gynecological conditions in healthy and diabetic women, as well as antibiotic users. The present study was conducted to determine the relationship between TUP1 gene expression patterns and symptomatic recurrent C. albicans infections.
Materials and Methods: This research was performed on C. albicans samples isolated from the vaginal specimens obtained from 31 individuals with RVVC in 2016. The reference strain C. albicans ATCC 10231, 10 C. albicans strains isolated from minimally symptomatic patients, and 10 isolates from asymptomatic patients were also used as control strains. The relative mRNA expression of the TUP1 gene was quantified using quantitative real-time polymerase chain reaction (QRT-PCR).
Results: The QRT-PCR results revealed that TUP1 mRNA expression was significantly decreased (0.001-0.930 fold) in the C. albicans isolates obtained from RVVC patients (p <0.001). However, no TUP1 expression was detectable in the isolates collected from asymptomatic patients. The results also indicated a significant correlation between TUP1 mRNA expression level and the severity of itching and discharge (p <0.001).
Conclusion: The present results were suggestive of the probable contribution of TUP1, as a part of the transcriptional repressor, to the severity of the symptoms related to C. albicans infections in the vagina. Regarding this, it is required to perform more in vivo studies using a larger sample size to characterize the regulatory or stimulatory function of TUP1 in the severity of RVVC symptoms. Furthermore, the study and identification of the genes involved in the severity of the symptomatic manifestations of C. albicans, especially in resistant strains, may lead to the recognition of an alternative antifungal target to enable the development of an effective agent.- انتشار مقاله: 27-07-1398
- نویسندگان: Mona Ghazanfari,Azam Fattahi,Mehraban Falahati,Majid Bakhshizadeh,Maryam Roudbary,Faramarz Masjedian Jazi,Mohsen Keykhosravi,Ensieh Lotfali
- مشاهده
- جایگاه : پژوهشی
- مجله: Current Medical Mycology
- نوع مقاله: Journal Article
- کلمات کلیدی: RT-PCR,Gene expression,MDR-1,C. albicans,CDR-2,Vulvovaginal candidiasis
- چکیده:
- چکیده انگلیسی: Background and Purpose: Candida albicans (C. albicans) is an opportunistic fungus that can colonize women’s mucosal epithelial cell surfaces, causing vulvovaginitis in specific circumstances. The major genes contributing to drug resistance in C. albicans are the candida drug resistance (CDR) and multi drug resistance (MDR) genes. The purpose of this study was to evaluate the CDR-2 and MDR-1 gene expression patterns in C. albicans strains isolated from patients with recurrent vulvovaginal candidiasis.
Materials and Methods: In this study, 40 isolates of fluconazole-resistant C. albicans were cultured on Sabouraud dextrose agar. These isolates were collected from women with vulvovaginitis who were referred to a clinic in Tehran, Iran, and transferred to a mycology laboratory. Then, RNA was extracted from the isolates using phenolchloroform and glass beads, and the complementary DNA (cDNA) was synthetized. To detect the semi-quantitative expression of CDR-2 and MDR-1 genes, the reverse transcriptase-PCR (RT-PCR) technique was performed using specific primers.
Results: Our findings indicated that of the 40 C. albicans isolates, 35 (87.5%) strains were positive for mRNA of the CDR-2 gene, 32 (80%) strains expressed mRNA of the MDR-1 gene, and 30 (75%) strains were confirmed to express mRNA of both the CDR-2 and MDR-1 genes simultaneously using the RT-PCR assay.
Conclusion: According to the obtained results, the expression rates of CDR-2 and MDR-1 genes were high in fluconazole-resistant C. albicans isolates, which can cause treatments to fail and result in chronic infections.Inhibiting these important genes using novel or natural agents can help with the treatment of chronic and recurrent vaginitis.
- انتشار مقاله: 18-04-1398
- نویسندگان: Kamal Khosravi Rad,Mehraban Falahati,Maryam Roudbary,Shirin Farahyar,Sanam Nami
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Lung cancer,β-glucans,Mesenchymal Stem Cells
- چکیده:
- چکیده انگلیسی: Objective: β-glucan, glucopyranosyl polymers of fungi cell wall, represent an immune stimulating effects with potential anti-cancer activity. Mesenchymal stem cells (MSC) have immunomodulating properties in cancer microenvironment. The aim of this study was to investigate the anti-cancer effect of Candida albicans (C. albicans) beta-glucan on MSCs supernatant for apoptosis assay of lung cancer cells in vitro. Methods: Beta-glucan was extracted from cell wall of C.albicans. MSC isolated from adipose tissue of patients and confirmed using specific surface markers expression which examined by flow cytometry. MSCs treated with various concentrations of β-glucans for 48 hours. Cytotoxic effect of β-glucans was evaluated using MTT assay. MSC and lung cancer line cocultured and treated with β-glucans and apoptosis assay was done by flow cytometry. Results: Cytotoxicity findings showed a significant decrease in MSC viability during 48h, however it was dose-dependent (P<0.05). According to the obtained findings, supernatant of mesenchymal stem cells treated with β-glucans increased cancer cells apoptosis (P<0.05). Conclusion: Beta glucan may highlight a potential and novel promising candidate in future strategies to cause apoptosis of cancer cells and consider as therapeutic agent against tumor growth as well. Definitely, more in vitro and in vivo studies are required to understand its functions.
- انتشار مقاله: 26-12-1397
- نویسندگان: Fatemeh Peymaeei,Fatemeh Sadeghi,Elahe Safari,Samaneh Khorrami,Mehraban Falahati,Shahla Roudbar Mohammadi,Maryam Roudbary
- مشاهده