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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Mesenchymal Stem Cells,Extracellular matrix,Scaffold,Large Intestine
- چکیده:
- چکیده انگلیسی: The aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. Rat large intestine was dissected in fragments and decellularized by physicochemical methods. The scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. Microscopic sections were prepared from the scaffolds after two weeks of culture with stem cells and studied by histological methods. The interactions of scaffolds with MSCs were also studied by electron microscopy. Histological and electron microscopy studies revealed human mesenchymal stem cell adhesion, migration, division and maintenance during the 14 days of culture in vitro. According to the results, scaffolds prepared from rat intestine matrix could be a suitable scaffold for studying in vitro cell behaviors such as division, migration and attachment. These various behaviors of cultured cells might be due to inductive effects of the extracellular matrix derived scaffold. However, more investigations are required to discover the exact effects of this scaffold and its interactions with mesenchymal stem cells.
- انتشار مقاله: 23-06-1394
- نویسندگان: Somayeh Naderi,Malihe Akbarzadeh Niaki,Nasser Mahdavi Shahri,Maryam Moghaddam Matin,Masoud Fereidoni,Fatemeh Naseri
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Bone and Joint Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Dog,Stem cells,Osteogenesis,Tissue engineering,Adipose tissue
- چکیده:
- چکیده انگلیسی: Background: Due to the known disadvantages of autologous bone grafting, tissue engineering approaches have become an attractive method for ridge augmentation in dentistry. To the best of our knowledge, this is the first study conducted to evaluate the potential therapeutic capacity of PRP-assisted hADSCs seeded on HA/TCP granules on regenerative healing response of canine alveolar surgical bone defects. This could offer a great advantage to alternative approaches of bone tissue healing-induced therapies at clinically chair-side procedures. Methods: Cylindrical through-and-through defects were drilled in the mandibular plate of 5 mongrel dogs and filled randomly as following: I- autologous crushed mandibular bone, II- no filling material, III- HA/TCP granules in combination with PRP, and IV- PRP-enriched hADSCs seeded on HA/TCP granules. After the completion of an 8-week period of healing, radiographic, histological and histomorphometrical analysis of osteocyte number, newly-formed vessels and marrow spaces were used for evaluation and comparison of the mentioned groups. Furthermore, the buccal side of mandibular alveolar bone of every individual animal was drilled as normal control samples (n=5). Results: Our results revealed that hADSCs subcultured on HA/TCP granules in combination with PRP significantly promoted bone tissue regeneration as compared with those defects treated only with PRP and HA/TCP granules (P<0.05). Conclusion: In conclusion, our results indicated that application of PRP-assisted hADSCs could induce bone tissue regeneration in canine alveolar bone defects and thus, present a helpful alternative in bone tissue regeneration.
- انتشار مقاله: 05-02-1396
- نویسندگان: Reyhaneh Shafieian,Maryam Moghadam Matin,Amin Rahpeyma,Alireza Fazel,Hamideh Salari Sedigh,Ariane Sadr-Nabavi,Halimeh Hassanzadeh,Alireza Ebrahimzadeh-Bideskan
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: fertility,Molecular markers,Differentiation,Self-renewal,Spermatogonial stem cell
- چکیده:
- چکیده انگلیسی: Spermatogonial stem cells (SSCs) also known as germ stem cells (GSCs) are the basis of spermatogenesis process in the testis. Furthermore, they are also valuable cells with different applications in developmental biology, transgenesis technology, and clinic. Understanding the new findings related to the cell and molecular biology of SSCs and the methods for isolation and maintenance of these cells are important and essential for their applications in medicine to treat some infertility problems and also in biotechnology to produce transgenic animals. The present review was conducted to describe the cell and molecular basis of development, self-renewal, and differentiation of mammalian and poultry SSCs in vivo (natural niche) and in vitro. Moreover, this study represents specific molecular markers to characterize SSCs. We also introduce methods to isolate, cultivate and enrich these cells, which are important for their applications. Finally, the significance of SSCs in different fields and their practical perspectives, and also the differentiation potential of other stem cells into spermatogonial- and spermatic-like cells are discussed.
- انتشار مقاله: 21-01-1394
- نویسندگان: Sajjad Sisakhtnezhad,Ahmad Reza Bahrami,Maryam Moghadam Matin,Fatemeh Behnam Rassouli,Madjid Momeni-Moghadam,Sohrab Boozarpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Bone,Dog,Stem cells,Osteogenesis,Tissue engineering,Adipose tissue
- چکیده:
- چکیده انگلیسی: Objective(s): Autologous bone transplantation known as the “gold standard” to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo.
Materials and Methods: hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination. In vivo, four cylindrical defects were drilled in the mandibular bones of 5 mongrel dogs and divided randomly into the following groups: I-autologous crushed bone, II- no filling material, III- HA/TCP and PRP, IV- PRP-enriched hAdMSCs seeded on HA/TCP granules. Inserted hAdMSCs were labeled to trace their contribution to bone tissue regeneration. Finally, cell tracing and tissue regeneration were evaluated by immunohistochemistry and histomorphometry methods, respectively.
Results: In vitro, co-incubation with HA/TCP granules significantly reduced proliferation and osteogenic differentiation ability of hAdMSCs; while PRP application promoted these capacities (P<0.05). In vivo, PRP-enriched hAdMSCs seeded on HA/TCP granules induced considerable bone formation in osseous defects (P<0.05). It was obviously shown that hAdMSCs were incorporated into the newly-formed bone.
Conclusion: Based on this study, application of stem cells could offer a helpful therapeutic tool in bone tissue regeneration. Although inserted hAdMSCs were identifiable throughout the newly-formed bone tissue, their few number could be an indicator of indirect role of hAdMSCs in tissue regeneration.- انتشار مقاله: 11-07-1396
- نویسندگان: Reihaneh Shafieian,Maryam Moghaddam Matin,Amin Rahpeyma,Alireza Fazel,Hamideh Salari Sedigh,Ariane Sadr-Nabavi,Halimeh Hassanzadeh,Alireza Ebrahimzadeh-Bideskan
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Valproic acid,COCl2,chemical treatment,CXCR4,CXCR7,Desferrioxamine,MSC,Homing
- چکیده:
- چکیده انگلیسی: Objective(s):The limited homing potential of bone-marrow-derived mesenchymal stem cells (BM-MSC) is the key obstacle in MSC-based therapy. It is believed that chemokines and chemokine receptor interactions play key roles in cellular processes associated with migration. Meanwhile, MSCs express a low level of distinct chemokine receptors and they even lose these receptors on their surface after a few passages which influence their therapeutic applications negatively. This study investigated whether treatment of BM-MSCs with hypoxia-mimicking agents would increase expression of some chemokine receptors and cell migration.
Materials and Methods: BM-MSCs were treated at passage 2 for our gene expression profiling. All qPCR experiments were performed by SYBR Green method in CFX-96 Bio-Rad Real-Time PCR. The Boyden chamber assay was utilized to investigate BM-MSC homing.
Results:Possible approaches to increasing the expression level of chemokine receptors by different hypoxia-mimicking agents such as valproic acid (VPA), CoCl2, and desferrioxamine (DFX) are described. Results show DFX efficiently up-regulate the CXCR7 and CXCR4 gene expression while VPA increase only the CXCR7 gene expression and no significant change in expression level of CXCR4 and the CXCR7 gene was detectable by CoCl2 treatment. Chemotaxis assay results show that pre-treatment with DFX, VPA, and Cocl2 enhances significantly the migration ability of BM-MSCs compared with the untreated control group and DFX treatment accelerates MSCs homing significantly with a higher rate than VPA and Cocl2 treatments.
Conclusion: Our data supports the notion that pretreatment of MSC with VPA and DFX improves the efficiency of MSC therapy by triggering homing regulatory signaling pathways.- انتشار مقاله: 10-11-1394
- نویسندگان: Hamid Reza Bidkhori,Naghmeh Ahmadiankia,Maryam Moghaddam Matin,Asieh Heirani Tabasi,Moein Farshchian,Hojjat Naderi-meshkin,Mina Shahriyari,Mahtab Dastpak,Ahmad Reza Bahrami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Tissue engineering,Acellular gingiva,Keratinocytes
- چکیده:
- چکیده انگلیسی: Objective(s)
Tissue engineering is an attractive science because it promises new therapeutic strategies for repairing organs that have lost functions due to damage. The purpose of this study was to evaluate induction effect of human gingival scaffold in tissue engineering for skin regeneration.
Materials and Methods
Tissue samples were obtained from crown-lengthening procedures and wisdom teeth removal. The samples were decellularized and used as a scaffold for loading of rat BM-MSCs. The human gingival scaffolds loaded by bone marrow mesenchymal stem cells were derived from Wistar rat. Finally, it was evaluated via electron micrographs, as well as immunohistochemical techniques at day 7, 14, and 28 after initial seeding.
Results
The histologic sections of human gingival scaffold –loaded rat BM-MSCs demonstrated formation of epithelial like layers at days 7, 14 and 28 after initial seeding. Scanning electron microscope (SEM) of the scaffolds indicated formed desmosomal adhesions, which revealed a degree of differentiation toward keratinocytes. The results of immunohistochemical staining were strongly positive for multi cytokeratin (CK) 14 days after initial seeding in epithelial differentiation. Rat BM-MSCs which loaded on human gingival scaffold is capable of differentiating toward keratinocytes.
Conclusion
Gingival tissues were presented as a natural scaffold for attachment and differentiation of bone marrow mesenchymal stem cells towards keratinocytes, and might be used as suitable scaffold for reconstruction of the skin.- انتشار مقاله: 28-06-1394
- نویسندگان: Nasser Mahdavishahri,Maryam Moghatam Matin,Masoud Fereidoni,Zahra Yarjanli,Seyed Ali Banihashem Rad,Saeedeh Khajeh Ahmadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Gamma Irradiation,Vinblastine,micronucleus assay,L929 cell
- چکیده:
- چکیده انگلیسی: Objectives: Vast number of studies show the relationship between aneuploidy and cancer. Ionizing radiation in
addition to induce all kinds of damages to the cells and structure of chromosomes, is also able to induce aneuploidy
through direct damages to chromosome division apparatus. Also irradiation of the cells induces mutations in several
genes which might be involved in cell division fidelity and play a role in reversing the effect of aneugens. Therefore,
irradiation of cells and tissues might produce sensitivity to agents with aneugenic capability in irradiated cells. Methods:
To investigate the persistent genomic effect of ionizing irradiation on chromosomal instability, L929 cells were gamma
irradiated with the dose of 2 Gy. Cells were left to recover from the harmful effect of irradiation. They were treated with
low dose of vinblastine (0.5 ng.ml-1) 72h post-gamma irradiation. Finally, the induced chromosomal abnormalities
were scored using micronucleus assay in cytokinesis-blocked binucleated cells (MnBi). Results: Irradiation-recovered
L929 cells treated with vinblastine showed a statistically higher frequency of MnBi compared to non-irradiated and
vinblastine treated cells. Conclusion: The results indicate that gamma irradiation, in addition to direct induction of
chromosomal damages, is also able to create persisting genomic sensitivity in the cells to chromosomal instability,
which is detectable when exposed to the second stimulus.- انتشار مقاله: 28-11-1396
- نویسندگان: Zahra Mohammadi,Farhang Haddad,Maryam M Matin,Shokouhozaman Soleymanifard
- مشاهده