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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Basal cell carcinoma,Immunohistochemical,Aggressiveness
- چکیده:
- چکیده انگلیسی: Background & Objective:Basal cell carcinoma (BCC) is classified into BCC1 or low risk (nodular, superficial type) and BCC2 or high risk (micronodular, morpheaform, infiltrative, and basosquamous types) based on clinical behavior. This study attempts to evaluate immunohistochemical (IHC) findings and clinical features associated with local aggressiveness and recurrence in BCC lesions.
Methods:This is a cross-sectional descriptive study conducted on 42 paraffin blocks (22 BCC1, 20 in BCC2) at Pathology Department of Afzalipour Teaching Hospital. First, demographic features of the patients were recorded and pathology blocks were classified by two dermatopathologists based on histopathological types of BCC1 and BCC2. Then, primary monoclonal antibodies including CD10, CD1a, SMA, Ki67, CD34, and P53 were utilized for IHC study. We compared BCC1 and BCC2 according to IHC markers, demographic features of patients, and tumoral features.
Results:The mean number of Langerhans cells (LCs) within epidermis above tumor mass was 14+1.92 and 4.7±1.23 in BCC1 and BCC2, respectively; these results show a significant difference between the two groups (P=0.001). P53 was positive in 41.13±6.39% and 74.5 ±6.26% of the tumor cells in BCC1 and BCC2 groups, which was statistically significant (P=0.001). Also, the mean number of blood vessels was 14.40±1.30 and 21.40±1.97 in BCC1 and BCC2, that was statistically significant (P=0.005).
Conclusion: Higher numbers of angiogenesis (SMA positive) and positive P53 were observed in BCC2 than BCC1. Also, more active positive CD1a cells were observed in BCC1 compared to BCC2.- انتشار مقاله: 20-12-1396
- نویسندگان: Simin Shamsi Meymandi,Shahriar Dabiri,Alireza ZeynadiniMeymand,Maryam Iranpour,Maryam Khalili,Sorour Alijani,Mahin Aflatoonian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: WOUND HEALING,immune system,Fibroblast,Allogeneic
- چکیده:
- چکیده انگلیسی: Background and Objective: In recent years, due to increasing number of patients with non-healing skin ulcers, skin substitutes have been used. Skin substitutes contain living cells causing faster and more effective wound healing. Therefore, research on the use of autologous and allogeneic cells such as fibroblasts in skin substitutes has attracted attentions. However, there are discrepancies in the immune responses to allogeneic fibroblasts. Therefore, we aimed to review the immune responses to allogeneic fibroblasts.
Methods: Donor fibroblasts were isolated from the skin of three rats. Nine recipient rats which were subcutaneously injected with three different regimens, were divided into three groups: Group 1; phosphate buffered saline (PBS) without cells (control), group 2: allogeneic fibroblasts of one animal source suspended in phosphate buffered saline, and group 3; phosphate buffered saline containing mixed allogeneic fibroblasts of three animal sources. The skin samples were biopsied at 1, 3 and 7 days after injection and studied histopathologically.
Results and Conclusion: No signs of redness and edema were observed in the injection sites. In pathology examination, changes such as vasculitis, eosinophils and lymphocytes accumulation around fibroblasts, fibroblast apoptosis and transplant rejection at the injection site were not observed in either group.
Subcutaneous injection of allogeneic fibroblasts in rats can be introduced as a promising approach for wound healing as they do not stimulate the immune system.- انتشار مقاله: 07-03-1396
- نویسندگان: Seyedeh Mehrnaz Kouhbanani Nejad,Farzaneh Armin,Shahriar Dabiri,Ali Derakhshani,Maryam Iranpour,Alireza Farsinejad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Dermatology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cutaneous lichen planus,Immune Cells,Immunohistochemistry (IHC)
- چکیده:
- چکیده انگلیسی: Background: Lichen planus (LP) is a common inflammatory skin disease. Pathogenesis of LP includes two possible mechanisms; related or unrelated to antigen. Regarding different clinical features of cutaneous and mucosal types of LP, for the first time, we decided to perform a qualitative and quantitative study of immune cells in different types of cutaneous LP and in comparison with normal skin. Methods: A total of 88 specimens (60 cases of cutaneous LP, 28 cases of normal skin) were selected from 2016 to 2017 in Kerman, Iran. Evaluation of immune cells was carried out based on qualitative and quantitative analysis. These findings were statistically calculated by descriptive statistical tests including frequency and mean ± standard deviation. Quantitative data were analyzed by independent t-test, chi-square, and analysis of variance (ANOVA). Data were analyzed using SPSS16 (SPSS Inc., Chicago, IL, USA). A p value less than 0.05 was considered statistically significant. Results: Our study demonstrated that the mean number of immune cells was significantly higher in lichen planus group in comparison with the control group. Number and staining intensity of Langerhans cells (LCs) in the LP group were significantly greater in epidermal than dermal region. Mastocytes were located mostly within the deep dermis in the LP group. Hypertrophic and atrophic LP had the highest and the lowest number of immune cells (i.e., mastocytes, LCs, and CD3 positive cells), respectively, with a significant difference. Conclusion: Our study demonstrated that immune cells were seen in larger numbers in the hypertrophic type of cutaneous LP which is consistent with the chronicity of this disease.
- انتشار مقاله: 02-08-1397
- نویسندگان: Meymandi Simin Shamsi,Dabiri Shahriar,Maryam Iranpour,Maryam Khalili,Mahin Aflatoonian
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: p53,Glioblastoma,JC Virus,HCMV,large T Antigen
- چکیده:
- چکیده انگلیسی:
Glioblastoma multiforme (GBM) is the most aggressive of the gliomas, a collection of tumors arising from glia in the central nervous system. Possible associations between the human cytomegalovirus (HCMV) and the JC virus with GBM are now attracting interest. Our present aim was to investigate the prevalence of the two viruses in Iranian patients from Kerman’s cities in the south of Iran. In addition, the expression rates of pp65, large T antigen and p53 proteins were assessed and their relation with GBM evaluated using reverse transcription real time PCR (rReal Time PCR) . A total of 199 patients with GBM cancer were enrolled, with mean±SD ages of 50.0±19.5 and 50.7±19.6 years for males and females, respectively. The P53 rate was dramatically low suggesting an aetiological role,. Large T antigen expression was found in JC positive samples, while the PP65 antigen was observed in patients positive for CMV and JC . HCMV products and JC virus with oncogenic potential may induce the development of various tumors including glioblastomas. The JC virus produces an early gene product, T-antigen, which has the ability to associate with and functionally inactivate well-studied tumor suppressor proteins including p53 and pRB .- انتشار مقاله: 13-06-1395
- نویسندگان: Reza Malekpour Afshar,Hamidreza Mollaei,Bahare Zandi,Maryam Iranpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Breast cancer,Kerman,Human papilloma virus
- چکیده:
- چکیده انگلیسی: Breast cancer is the first of the most important causes of the deaths of women in the world and in Iran. There are
various causes and causes of this cancer, one of which has recently been described as a cause of breast cancer, is the human
papillomavirus (HPV). The HPV is transmitted through sexual contact and skin lesions. There are more than 100 types of
HPV that can influence different parts of the body. Some types of HPV can cause cancer (such as cervical or anal cancer)
and others can cause warts (such as genital or plantar warts). To study the risk of HPV infection in Breast Cancer, we
managed a Case-Control study in Kerman, southeast of Iran. For this purpose, 98 paraffin blocks of breast cancer and
40 paraffin blocks of fibrocystic as a control were tested for the presence of HPV DNA using Real-Time PCR, and
HPV typing was done using INNo-Lippa assay. HPV DNA was detected in 8 out of 98 patients (8.2%), while it was
not detected in the control group samples. HPV types 16, 18 were the most common (62.5%) types in positive samples.
The prevalence of HPV in patients with breast cancer of Iran is very low and less than other regions of the world,
it seems that maybe rout of transmission of HPV in Iran is under control. No one knows exactly why breast cancer
occurs. The environment, hormones, Viruses, or your lifestyle could all play a role in the development of breast cancer.
Currently, Vaccination is the best way to prevent cancer that’s due to HPV. However, additional studies on the larger
group of patients are needed to explain the roles of HPV in Breast cancer.- انتشار مقاله: 03-07-1396
- نویسندگان: Reza Malekpour Afshar,Negin Balar,Hamid Reza Mollaei,Seyed Alimohammad Arabzadeh,Maryam Iranpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,HPV,Real Time PCR,HPV Genotype
- چکیده:
- چکیده انگلیسی:
Background: The HPV virus is known to be oncogenic and associations with many cancers has been proven. Although many studies have been conducted on the possible relationship with colorectal cancer (CRC), a definitive role of the virus has yet to be identified. Method: In this cross-sectional study, the frequency of HPV positivity in CRC samples in Kerman was assessed in 84 cases with a mean age of 47.7 ± 12.5 years over two years. Qualitative real time PCR was performed using general primers for the L1 region of HPV DNA. Results: Out of 84 CRC samples, 19 (22.6%), proved positive for HPV DNA. Genotyping of positive samples showed all of these to be of high risk HPV type. Prevalence of HPV infection appears to depend geographic region, life style, diet and other factors. Conclusion: In our location frequency of CRC is low, and this limited the sample size for evaluation of HPV DNA. The most prevalent types were HPV types 51 and 56. While HPV infection may play an important role in colorectal carcinogenesis, this needs to be assessed in future studies.- انتشار مقاله: 11-07-1396
- نویسندگان: Reza Malekpour Afshar,Zeinab Deldar,Hamid Reza Mollaei,Seyed Alimohammad Arabzadeh,Maryam Iranpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Interleukins,Antioxidants,Water pipe smoking,Lung injury,Swimming
- چکیده:
- چکیده انگلیسی: Background: The use of waterpipe tobacco smoking (WTS) is on the rise throughout the world, especially
among young people and even athletes. There is a belief among consumers that exercise prevents the harmful
effects of hookah smoke on the body. We examined this belief by evaluation of lung injury following to
concurrent WTS and swimming endurance training in male Wistar rats.
Methods: Animals were randomly divided to sedentary control (CTL) group, exercise training group
(Ex group), sedentary WTS (S) group, and exercise plus WTS (S + Ex) group.
Findings: 8 weeks of WTS was associated with significant increase in serum level of cotinine, lung damage,
reduction in alveolar number AN/SA (mm2) and increase in malondialdehyde (MDA) level of lung tissue.
Combination of exercise with WTS significantly decreased these negative effects; however, it could not fully
protect the lung from smoking damage. Waterpipe smoking (WPS) also significantly increased the proinflammatory cytokines of lung tissue such as tumor necrosis factor alpha (TNF-α) (P < 0.001), interleukin 1
beta (IL-1β) (P < 0.010), and IL-6 (P < 0.050) in comparison with CTL group. Exercise training to some
degree reduced the levels of pro-inflammatory cytokines and increased the level of IL-10 as an antiinflammatory IL and glutathione peroxidase (GPX) activity in animals exposed to WTS.
Conclusion: It is suggested that combination of mild to moderate exercise with WTS may attenuate the
hookah smoking-induced lung damage. This effect partly is mediated through balancing of pro/antiinflammatory and redox systems
- انتشار مقاله: 10-04-1399
- نویسندگان: Mohammad Reza Nakhaee,Mohammad Reza Zolfaghari,Siyavash Joukar,Nouzar Nakhaee,Yaser Masoumi-Ardakani,Maryam Iranpour,Mozhdeh Nazari
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Smoking water pipes,Exercise training,Left ventricular function,Antioxidants
- چکیده:
- چکیده انگلیسی: Background: There is an increasing popularity of waterpipe tobacco smoking (WTS) in youth and even in
athletes worldwide. Despite the existence of evidence of the harmful effects of hookah smoke on various
systems of the body, especially the cardiovascular system, its simultaneous effect with exercise training has
not been well studied. We assessed the effects of WTS exposure with/without swimming exercise on blood
pressure (BP), and heart histology and mechanical performance in male Wistar rats.
Methods: The animals were divided into 4 groups of sedentary control (CTL), waterpipe tobacco smoking (S),
mild endurance swimming exercise training (Ex), and waterpipe smoking plus exercise (S + Ex). The
duration of WTS and exercise was 8 weeks.
Findings: BP and heart rate (HR) did not show a significant difference among the groups. WTS increased the
TNF-α level of the heart (P < 0.05 vs. CTL) and cardiac tissue lesions (P < 0.05 vs. CTL), and reduced +dP/dt
max, -dp/dt max, and heart contractility indices (P < 0.01, P < 0.01, and P < 0.05, respectively, vs. CTL and
Ex groups). It also increased the Tau index (P < 0.05 vs. CTL; P < 0.01 vs. Ex groups) of the left ventricle.
However, the combination of exercise and WTS reduced the TNF-α level, improved the heart activity of
superoxide dismutase (SOD) and catalase enzymes, and prevented the negative effects of smoking on heart
function and morphology.
Conclusion: Mild exercise prevents WTS-induced left ventricular systolic and diastolic dysfunction partly via
improvement of antioxidants and attenuation of pro-inflammatory cytokines.
- انتشار مقاله: 15-04-1398
- نویسندگان: Mohammad Reza Nakhaee,Siyavash Joukar,Mohammad Reza Zolfaghari,Farzaneh Rostamzadeh,Yaser Masoumi-Ardakani,Maryam Iranpour,Mozhdeh Nazari
- مشاهده