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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,hwp1,vaginal Candidiasis,adherence
- چکیده:
- چکیده انگلیسی: Candida albicans is an opportunistic fungal pathogen found as mycoflora in the human body surfaces. Sevral genes play a crucial rule in its virulence including Hwp1 (hyphal wall protein 1), BCR1 and ALS gene family. Hwp1 gene is a responsible for coding a cell surface protein, which mediates biofilm formation in candida albicans. Here we investigated the presence of the HWP1gene was characterised among Candida albicans isolates in women with recurrent vaginal canididasis and further we studies its role in cell adherence. We used 50 Candida albicans clinical isolates resistant to Fluconazole. RNA (of samples were extracted using glass bead and lysis buffer and cDNA was synthesized using reverse transcriptase enzyme. Expression of (HWP1) gene was analysed using reverse transcriptase-plolymerase chain reaction (RT-PCR). The ability of adherence of the isolates with or without the expression of HWP1 were characterized using Hela cells. Statistical analysis were performed using t-test and two-way ANOVA SPSS software. Our results showed that the HWP1 gene were present in 47 samples (94%) out of 50 isolates, 27 samples (57%) had expression of HWP1. The result of adherence assay revealed that the isolates with the expression of HWP1 gene and control isolates was statistically different (p<0.05). In conclusion, the isolates with the expression of HWP1 gene has the higher ability to adhere the epithelial mucosal cell surface. Our data support a positive correlation between the expression of HWP1 gene and the ability of adherence to epithelial cells.
- انتشار مقاله: 27-03-1392
- نویسندگان: Maryam Hosseini Por,Shahla Roudbarmohammadi,Maryam Roudbary,Bita Bakhshi,Zahra Farhadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Foreign Language Teaching & Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Domestication,AVT,Culture-Specific Items,taboo,Foreignization
- چکیده:
- چکیده انگلیسی: Translators face a number of difficulties while translating. Not only translation of cultural elements is of utmost importance in translation studies, but also it is an arduous task for translators, especially in the case of interlingual translation. One of the biggest concerns of audiovisual translators is culture-specific items amongst which taboo expressions are included. This study aimed at investigating the applied strategies in the translation of taboo expressions, from English into Persian, in 5 Hollywood dubbed movies by IRIB; namely, Family Business (1989), Lethal Weapon 3 (1992), Sudden Death (1995), Welcome to Collinwood (2002), and Anacondas (2004). Establishing this research, the original movies were compared with their dubbed Persian versions. Hence, the taboo expressions were extracted and compared with their Persian translations in the dubbed versions. Based on Venuti’s (1995) strategies in translation studies (i.e., domestication and foreignization), the results were interpreted. Analysis of the relevant data indicated χ2 (12, N = 5) = 23.08, p = 0, considering **p ˂ 0.05. Moreover, the “deletion” strategy, with the highest frequency (i.e., 55.97%), was the topmost used strategy in the rendition of the taboo expressions.
- انتشار مقاله: 10-10-1394
- نویسندگان: Mahmood Hashemian,Azizullah Mirzaei,Maryam Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: fatty acids,Antibacterial,Essential oil composition,Ligularia persica,Asteraceae
- چکیده:
- چکیده انگلیسی: Objectives: The objective of this research was to investigate the chemical composition and antibacterial activities of the fatty acids and essential oil from various parts of Ligularia persica Boiss (L. persica) growing wild in north of Iran.
Materials and Methods: Essential oils were extracted by using Clevenger-type apparatus. Antibacterial activity was tested on two Gram-positive and two Gram-negative bacteria by using micro dilution method.
Results: GC and GC∕MS analysis of the oils resulted in detection of 94%, 96%, 93%, 99% of the total essential oil of flowers, stems, roots and leaves, respectively. The main components of flowers oil were cis-ocimene (15.4%), β-myrcene (4.4%), β-ocimene (3.9%), and γ-terpinene (5.0%). The major constituents of stems oil were β-phellandrene (5.4%), β-cymene (7.0%), valencene (3.9%). The main compounds of root oil were fukinanolid (17.0%), α-phellandrene (11.5%) and Β-selinene (5.0%) and in the case of leaves oil were cis-ocimene (4.8%), β-ocimene (4.9%), and linolenic acid methyl ester (4.7%). An analysis by GC-FID and GC-MS on the fatty-acid composition of the different parts of L. persica showed that major components were linoleic acid (11.3-31.6%), linolenic acid (4.7-21.8%) and palmitic acid (7.2-23.2%). Saturated fatty acids were found in lower amounts than unsaturated ones. The least minimum inhibition concentration (MIC) of the L. persica was 7.16 μg/ml against Pseudomonas aeruginosa.
Conclusion: Our study indicated that the essential oil from L. persica stems and flowers showed high inhibitory effect on the Gram negative bacteria. The results also showed that fatty acids from the stems and leaves contained a high amount of poly-unsaturated fatty acids (PUFAs).- انتشار مقاله: 12-10-1393
- نویسندگان: Maryam Mohadjerani,Rahman Hosseinzadeh,Maryam Hosseini
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Recurrence,cervical intraepithelial neoplasia,Neutrophils,Lymphocytes,Blood Platelets
- چکیده:
- چکیده انگلیسی: Background: Immune system status is a factor related to cervical intraepithelial neoplasia (CIN) recurrence.
neutrophil-lymphocyte ratio (NLR) is a useful factor in assessing the immune status. The aim of this study was to
evaluate the prognostic value of NLR factor for CIN recurrence in patient who underwent excisional procedure and its
relationship with recurrence free survival (RFS). Methods: In this historical cohort study, a population of 307 patients
with CIN (confirmed by excisional pathology) from 2009 to 2017 were selected. NLR and hematologic factors were
measured before surgery and the follow-up records of these patient were analyzed. The recurrence rate and RFS were
assessed during the follow-up phase. Results: The NLR cut-off point of 1.9 was determined using the Youden Index.
NLRUnivariate analysis showed that higher NLR values (P<0.001), absolute neutrophilic counts (ANC) (P<0.001) and
platelet lymphocytic ratios (PLR) (P=0.002) were significantly associated with reduction in RFS. The results of Cox
regression showed that removing more tissue during excision (HR = 0.325; 95% CI (0.936-0.136) significantly reduced
the hazard of recurrence, higher NLR (HR = 4.55; 95% CI) (1.97-10.51) and white blood cell (WBC) count levels (HR
=1.27; 95% CI, 1.04-1.55), significantly decreased RFS, but PLR and ANC associated with RFS were not confirmed
by Cox regression. Conclusion: NLR and total WBC count might be prognostic factors involved in the prediction of
recurrence and RFS in CIN patient underwent excisional procedure. To confirm these results, more prospective studies
with larger sample sizes are needed.- انتشار مقاله: 08-12-1397
- نویسندگان: Farah Farzaneh,Nafiseh Faghih,Maryam Sadat Hosseini,Maliheh Arab,Tahereh Ashrafganjoei,Atyeh Bahman
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: cervical cancer,Papanicolaou Smear,Anal Neoplasms,Anal Canal Abnormalities,Cervical Intraepithelial Neoplasms
- چکیده:
- چکیده انگلیسی: Background: Anal cancer is uncommon. Although uncommon, the incidence of anal cancer has increased among
the general population of the United States and other countries over the past 30 years. We evaluated anal cytology in
women with the history of abnormal pap smear, cervical intraepithelial neoplasia, cervical cancer and high risk HPV
for anogenital dysplasia. Methods: In this cross-sectional study, 153 patients over the age of 21 years, referred to Imam
Hossein Hospital in 1395-1396 who were being monitored due to abnormal pap smear, cervical cancer, CIN and high
risk HPV, were evaluated with anal cytology for anogenital dysplasia. Results: 153 patients were enrolled in four
main groups. Among those with a history of abnormal pap smear (39 patients), only 25% of the patients with HSIL
had abnormal anal pap smear, and the rest of them had negative anal smear. Among those with a history of CIN (61
patients), patients with CIN III, 30.8% had abnormal anal smear, while the rest had a negative anal smear. Among those
with a high risk of HPV (23 patients), 50% of the patients with concurrent positive HPV 16 and 18 had anal abnormal
smear. Patients with a history of cervical cancer (30 patients) who had radiotherapy with or without surgery showed a
negative anal smear. The ratio of anal SIL positive of individuals with a sexual partner was only 1.9%, while this ratio
was 27.3% for those with more than one partner and this difference was statistically significant (P <0.0001). 15.4%
of smokers had positive anal SIL test result, while, only 2.9% non-smokers had the same result, and this difference
was statistically significant (P = 0.03). 20% of the patients who had anal intercourse showed a positive anal SIL score
result, compared to 1% for those who did not. This difference was statistically significant (P <0.0001). Conclusion:
Among people with a history of abnormal cervical pap smear, CIN, cervical cancer and high-risk HPV during the last
6 months, abnormal anal tests are most commonly seen in patients who had risk factors such as anal sex, cigarette,
multi partner etc. with high grade interepithelial squamous cervix such as HSIL, CIN III, HPV 16 and 18; more studies
in larger sample size are needed to have the better conclusion.- انتشار مقاله: 09-08-1396
- نویسندگان: Maryam Sadat Hosseini,Donya Khosravi,Farah Farzaneh,Abdalali Ebrahimi,Maliheh Arab,Tahereh Ashraf Ganjoie,Farzane Jamdar,Atefeh Moridi,Mohammad Chehrazi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Optimal Debulking,Neoadjuant Chemotherapy,CA125,Prediction Model
- چکیده:
- چکیده انگلیسی: Background: Primary cytoreduction surgery followed by chemotherapy is the cornerstone treatment for epithelial
ovarian cancer (EOC). In patients with a low probability of optimal primary surgical debulking, neoadjuvant
chemotherapy (NACT) followed by interval debulking increases the chance of optimal surgery. The aim of this study
was to develop a model to identify preoperative predictors for suboptimal cytoreduction. Methods: Medical records
of patients with EOC who underwent primary cytoreductive surgery in a referral tertiary gyneco-oncology center
were reviewed from 2007 to 2017. Data were collected on a range of characteristics including demographic features,
comorbidities, serum tumor markers, hematologic markers, preoperative imaging, surgical procedures, and pathologic
reports. Univariate and multivariate analyses were performed to clarify the ability of preoperative factors to predict
suboptimal primary surgery. Results: The majority of patients (71.3%) who underwent primary cytoreductive surgery
were optimally debulked. Based on the Youden index, the best cut-off point for the serum CA125 level to distinguish
suboptimal debulking was 420U/ml with 0.730 (95%CI:0.559 to 0.862) sensitivity and 0.783 (0.684 to 0.862) specificity.
Multiple logistic regression results showed that serum CA125 level >420 U/ ml (p value <0.001), the presence of liver
metastasis on preoperative imaging (p value: 0.041) and ascites (p value: 0.032) or massive ascites (p value:0.010)
significantly increased the risk of suboptimal debulking (logit p = 2.36 CA125 level +1.85 Liverinvolvement +1.68
presence of Ascites+ 2.28 Massive Ascites). Conclusion:The present study suggests that a serum CA125 level >420 U/ml,
the presence of ascites or massive ascites and liver metastasis are strong predictors of suboptimal primary surgery in
cases of EOC. Based on the constructed model, with any of these 4 factors, the probability of suboptimal debulking in
EOC is more than 80%.- انتشار مقاله: 05-09-1396
- نویسندگان: Maliheh Arab,Farzane Jamdar,Maryam Sadat Hosseini,Robabe Ghodssi-Ghasemabadi,Farah Farzaneh,Tahereh Ashrafganjoie
- مشاهده
- جایگاه : پژوهشی
- مجله: Advances in Nanochemistry
- نوع مقاله: Journal Article
- کلمات کلیدی: Chitosan,5-Fluorouracil,Carbon nanotubes,Copper nanoparticles
- چکیده:
- چکیده انگلیسی: A fast and facile electrochemical sensor for detection of important anti-cancer drug 5-fluorouracil (5-FU) is fabricated using a copper-nanoparticles decorated multi-walled carbon nanotube and chitosan composite modified electrode. Copper nanoparticles/multi-walled carbon nanotubes/ionic liquid/chitosan modified glassy carbon electrode (CuNPs/MWCNTs/IL/Chit/GCE) prepared by the consecutive coating of MWCNTs/IL/Chit nanocomposite on the GCE, followed by the electrodeposition of copper. Surface characteristics of the modified electrode were studied by scanning electron microscopy (SEM). The electrochemical capability of the fabricated modified electrode for the detection of 5-FU is examined by cyclic voltammetry, differential pulse voltammetry (DPV). The nano Cu decorated MWCNTs/IL/Chit/GCE is found to be efficient for the electrocatalytic oxidation of 5-FU. The peak current of the DPV exhibited a linear relationship against 5-FU over a wide concentration range of 1-110 µM with a low detection limit (0.15 µM). Additional, the sensor was successfully applied in biological fluid sample analysis.
- انتشار مقاله: 20-03-1398
- نویسندگان: Mahmoud Roushani,Zahra Saeidi,Sara Hemati,Maryam Hosseini
- مشاهده