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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: genotyping,Epstein-Barr virus (EBV),EBV Nuclear Antigen (EBNA),hematologic malignancy
- چکیده:
- چکیده انگلیسی: Background: Epstein-Barr virus (EBV) is associated with different malignant diseases, such as Hodgkin lymphoma (HL) and lymphoproliferative disorders. Patients with hematologic malignancies by variable severity could be suspected for the infection with different types of this virus. This preliminary study reported the genotyping and related viral load of Epstein-Barr virus in Iranian patients with hematologic malignancies for estimation of possible factors affecting malignancy. Methods: Peripheral blood mononuclear cells (PBMC) of HL (n=20), NHL (n=29), acute lymphocytic leukemia (ALL) (n=18) and chronic lymphocytic leukemia (CLL) (n=12) were obtained. After DNA extraction, a nested-PCR and a conventional-PCR targeting EBNA-2 and EBNA-3C genes were performed. A real-time PCR assay for viral load quantitation carried out. Standard curve analysis used for evaluation of amplification specificity. Results: Of 79 included patients, 34 (43%) were EBV positive. There were 23.5% (8/34), 38.2% (13/34), 23.5% (8/34), 14.8% (5/34) in HL, NHL, ALL and CLL groups, respectively. Also, the main genotype was genotype I (91.2%) which it follows by 8.8% (3/34) genotype II. The real-time PCR assay showed the mean viral load ± std. deviation was 2.75×105 ± 1.202×106 copies/μg DNA and the higher viral load was seen in NHL patients. Conclusion: This preliminary investigation in Iran shows that the main EBV genotype into our region probably is genotype I (91.2%) which it is similar to others. We could not find any statistically significant association between the virus infection and viral load with any specific disease and patients’ demographic data.
- انتشار مقاله: 21-07-1398
- نویسندگان: Alireza Tabibzadeh,Mohammad Hadi Karbalaie Niya,Maryam Esghaei,Farah Bokharaei-Salim,Angila Ataei-Pirkooh,Seyed Jalal Kiani,Seyed Hamid Reza Monavari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Cisplatin,Tea,Camellia sinensis,Caco-2 Cells,Aquaporin 5
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is widespread across the world. While conventional anticancer treatments
can help the affected patients, cells of vital organs such as the kidney, lungs, bladder and nervous system may
suffer from side effects of chemotherapeutic drugs, so that it is necessary to search for alternatives. From ancient
times, attention has focused on medicinal plants and natural products. In the current work, Camellia sinensis, whose
leaves are used to produce green tea was evaluated for anticancer effects in cell culture. Materials and Methods:
A hydroalcoholic extract of Camellia sinensis young leaves was prepared by percolation and compared with Cisplatin
as a known anticancer drug for effects on two cell lines: Caco-2, colon carcinoma cells, and mouse normal fibroblasts
(L929). Cytotoxicity of 50, 100, 200, 400 and 800 μg/ml of Camellia sinensis extract was evaluated by MTT assay and
aquaporin 5 (AQP5), detected as a biomarker for surviving cells using immunofluorescence microscopy. Results: MTT
assays with hydroalcoholic extract of Camellia sinensis showed considerable inhibition of growth of Caco-2 cells,
significant at 800 μg/ml (P<0.05), with little effect on L929 cells. Levels of aquaporin 5 protein decreased in Caco-2
cell culture following green tea extract treatment. Conclusion: According to the results of the current study, Camellia
sinensis is a medicinal plant with potent anticancer influence which might be specific.- انتشار مقاله: 09-11-1396
- نویسندگان: Maryam Esghaei,Hadi Ghaffari,Bahman Rahimi Esboei,Zienab Ebrahimi Tapeh,Farah Bokharaei-Salim,Manijeh Motevalian
- مشاهده