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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: 5-Fluorouracil,Melanoma,Myeloid Derived Suppressor Cells
- چکیده:
- چکیده انگلیسی: Background: Melanoma progression and metastasis is suggested to be mediated by increased accumulation of myeloid derived suppressor cells. Various chemotherapeutic drugs such as 5-Fluorouracil in single low concentration have the capacity, at least in part, to reverse tumor progression by reducing myeloid derived suppressor cellsmediated immunosuppression.
Objective: To assess whether multiple low doses of 5- fluorouracil could repress myeloid derived suppressor cells in low frequency and, in turn, could enhance anti-tumor responses and promote a more prolonged survival in a murine melanoma model.
Methods: Fifty milligram per kilogram body weight dose of 5-Flourouracil was administered intraperitoneally 4 times with 3-day intervals to C57BL/6 mice after B16 melanoma tumor models were established. The frequency and suppressive functions of myeloid derived suppressor cells and induction of anti-tumor CD8+ T cells as well as tumor growth and survival were evaluated in drug treated and untreated mice.
Results: Our results demonstrated that this therapeutic strategy increases the overall mice survival (p≤0.01) and induces melanoma-specific CD8+T cell immunity (p≤0.01) by reducing the frequency of myeloid derived suppressor cells (p≤0.01) as well as their immune suppressive functions (p≤0.05).
Conclusion: Altogether, our data suggest that 5-fluorouracil in multiple low regimens might be used to overcome tumor immunosuppression and improve the efficacy and outcome of antitumor immune responses in a mouse model.- انتشار مقاله: 14-05-1395
- نویسندگان: Afshin Namdar,Hamid Reza Mirzaei,Farhad Jadidi-Niaragh,Mahboubeh Ashourpour,Maryam Ajami,Jamshid Hadjati,Abbas Rezaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: fennel,Asafetida,Ginseng,4T1 Cell line,Breast cancer
- چکیده:
- چکیده انگلیسی: Background: The 4T1 cells tumor growth and metastatic pattern in BALB/c mice very closely mimic human breast cancer. These herbal remedies used in traditional folk medicine have been the source of many medically beneficial drugs. The aim of the current study was to evaluate the anti-proliferative activity of the asafoetida, ginseng and fennel ethanolic extracts on mouse breast cancer 4T1 cell-line invitro.Method: in this experimental study, Asafoetida, gensing and fennel were extracted; 4T1 mouse mammary tumor cell line were cultured in 48-well flat bottom plate in density of 50x103 per well in 100 µl RPMI1640 medium then different dilutions of each extract (25 , 50, 100, 200, 500, and 2000 μg/ml) were added to cell culture. Cells then were incubated at 37 oC for 24 hours. After 24 h, cell Proliferation was determined by the BRDU assay . dataset of experiments were collected and analysed with SPSS19 software. Significant level of Data was Examined with one-way ANOVA method and P
- انتشار مقاله: 13-11-1393
- نویسندگان: Amir Hossein Mansourabadi,Ali Shams,Reza Mansouri,Ahmad Najafi,Maryam Ajami
- مشاهده