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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Lymphocytes,Spontaneous Adenocarcinoma,Structural Proteins,Secreted Proteins
- چکیده:
- چکیده انگلیسی: Background: Candida albicans is one of the most important opportunistic pathogens that suppress immunologic mechanisms of the host. It is speculated that structural and secretory proteins of C. albicans have immunomodulatory effects in cancer.
Objective: To evaluate the effects of C. albicans structural and secreted proteins on intratumoral CD4/CD8 ratio as well as the survival rate in BALB/c tumor model.
Methods: Structural and secretory proteins from C. albicans were isolated and examined for their effects on tumor growth and survival of adenocarcinoma bearing mice.
Results: The results indicated that in mice treated with C. albicans structural protein, the survival rate significantly decreased compared with the control groups. Also, mice treated with secretory proteins showed a decrease in survival rate but it was not statistically significant (p>0.05). Investigating the frequency of tumor infiltrated CD4+ and CD8+ T lymphocytes indicated that the percentages of tumor infiltrated CD4+ T lymphocytes in response to structural and secreted proteins were higher compared to the control groups.
Conclusion: Our study suggests that C. albicans structural and secreted proteins modulate intratumor T lymphocyte infiltration.- انتشار مقاله: 16-05-1395
- نویسندگان: Marzieh Holakuyee,Mohammad Hossein Yadegari,Zuhair Mohammad Hassan,Mansour Bayat,Ariyo Shahin Jafari,Mohsen Abolhassani,Abbas Ali Amini,Mehdi Mahdavi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Aflatoxin B1,FRET,Magnetic/Silica Core shell,Nanobiosensor
- چکیده:
- چکیده انگلیسی: Background: Recently, some new nanobiosensors using different nanoparticles or microarray systems for detection of mycotoxins have been designed . However, rapid, sensitive and early detection of aflatoxicosis would be very helpful to distinguish high-risk persons. Objectives: We report a highly sensitive competitive immunoassay using magnetic/silica core shell as a signal intensifier for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET) from Cd/Te quantum dots (anti-aflatoxin B1 antibody immobilized on the surface of Cd/Te quantum dots) to Rhodamine 123 (Rho 123-labeled aflatoxin B1 bound to albumin). The specific immune-reaction between the anti-aflatoxin B1 antibody on the QDs and the labeled-aflatoxin B1 brings the Rho 123 fluorophore (acting as the acceptor) and the QDs (acting as the donor) in close spatial proximity and causes FRET to occur upon photo-excitation of the QDs. Using magnetic/silica core shell to intensify the obtained signal is the novelty of this study. Materials and Methods: Cd/Te QDs were synthesized by the simultaneous reduction of cadmium chloride and tellurium in the presence of sodium borohydride under nitrogen atmosphere. Magnetic nanoparticles were synthesized using FeSO4 and FeCl3 (1:2 molar ratio) and ammonia as an oxidizing agent under nitrogen atmosphere. The prepared magnetic nanoparticles shelled by silica using tetraethoxysilane in the presence of ammonia. Nanoparticles synthesis and monodispersity confirmed by TEM. Immobilization of Cd/Te QDs to antibodies and labeling of aflatoxin B1-albumin by Rho 123 were performed by EDC/NHS reaction in reaction mixture buffer, pH 6, at room temperature. Results: By using the magnetic/silica core shell sensitivity of the system changed from 2×10-11 in our previous study to 2×10-12 in this work. The feasibility of the method established by the detection of aflatoxin B1 in spiked human serum. There is a linear relationship between the decreased fluorescence intensity of Rho 123 with increasing concentration of aflatoxin B1 in spiked samples, over the range of 0.01-0.06 mmol.mL-1. Conclusion: This homogeneous competitive detection scheme is simple, rapid and efficient, and does not require multiple separation steps and excessive washing.
- انتشار مقاله: 03-12-1393
- نویسندگان: Alireza Kalarestaghi,Mansour Bayat,Seyed Jamal Hashemi,Vadood Razavilar
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: aspirin,Real-time PCR,Fluconazole,Candida glabrata,ERG11
- چکیده:
- چکیده انگلیسی: Azole compounds have been a treatment option for Candida glabrata infections. However, azole resistance can occur through different mechanisms such as alterations in ERG11 (lanosterol 14α-demethylase). Aspirin (ASA), a non-steroidal anti-inflammatory drug, showed antimicrobial activity against Candida. The purpose of this study was to evaluate the synergistic effect of ASA as an anti-inflammatory drug with the fluconazole on the azole-resistant C. glabrata isolates. In the cross-sectional study, a total of 60 oral samples were collected from the school of dentistry, Tehran University of medical sciences. After confirmation of fungal isolates, template DNA was extracted and PCR for detection of Erg11 gene by specific primers was performed in all Fluconazole-resistant isolates. MIC for ASA was determined using broth dilution method in 96‐well plates. RNA was extracted and cDNA synthesized according to the Omniscript RT kit instructions. The effect of ASA in the treated and non-treated groups on ERG11 gene expression was determined by Real-Time PCR technique. Out of 60 collected samples, 12 (20%) C. glabrata were isolated. All of these isolates were resistant to fluconazole and carried ERG11 genes. Real-time PCR results showed that the combination of ASA with fluconazole reduced ERG11 gene expression. It is concluded that, treatment of candidal infections with ASA significantly reduced resistance in to azole compounds by down expression of ERG11 gene, suggesting that an NSAID might be useful for azole-resistance candidal infections.
- انتشار مقاله: 14-01-1398
- نویسندگان: Zahra Khosravi,Kumarss Amini,Mansour Bayat,Laya Takbiri Osgoei
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: 4-Chlorophenol,Degradation,Wastewater,Imam Khomeini seaport,petrochemical
- چکیده:
- چکیده انگلیسی: Chlorophenols are the most toxic pollutants of water and wastewater. Since 4-chlorophenol is a high soluble compound in water, it is found in water and wastewater abundantly. Because of high costs, high energy consumption and in some cases environmental inconsistency in chemical and physical removal methods, biochemical degradation of 4-chlorophenol is very important. In the present study, 13 strains of bacteria and 6 strains of yeast and mold were purified and isolated from wastewater treatment plant (Imam Khomeini seaport, Mahshahr), which lasted about 15 days. Then, the ability of each microorganism isolated in the presence of 100ppm of 4-chlorophenol was studied and two microbial species suitable for TY1 and TY2 were selected for use in mixed microbial culture. In this research, one of the most important factors affecting 4-chlorophenol degradation was by mixed microbial culture including glucose concentration with 2 and 5g/l was investigated. After examination, the microbial strains suitable for TY1 and TY2 were able to completely remove the 100ppm of 4-chlorophenol, so that the TY1 strain was removed completely after 45 hours and by TY2 strain after 21 hours and also, using a mixture of TY1 and TY2 strains (50/50) and in the presence of 2g/l glucose, 100ppm of 4-chlorophenol was completely removed after 18 hours. The significance and impact of this study was the use of indigenous strains isolated from wastewater treatment plant in petroleum refineries and petrochemical industries for the biodegradation of chlorophenol.
- انتشار مقاله: 04-10-1396
- نویسندگان: Atena Alirezaei Dizicheh,Mansour Bayat,Mahmood Alimohmmadi,Jamal Hashemi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Detoxification,Mycotoxin,Penicillium,Citrinin,Cold Atmospheric Pressure Plasma
- چکیده:
- چکیده انگلیسی: Mycotoxins such as Citrinin are small toxic molecules produced by a great variety of microorganism, which encompass several classes of secondary metabolites with no common chemical structure or mode of action. Citrinin produces by different species of Penicillium. Today, the cold atmospheric pressure plasma (CAPP) method has a potential for mycotoxin detoxification. In this study, a new and promising approach involving the use of cold atmospheric pressure plasma is considered, which may overcome multiple weaknesses associated with the classical methods.
In this study, samples included wheat, corn; rice and Barley were obtained from northern parts of Iran (Lahijan). Initially, the output of the power supply was set to 50 kV, 100 watts, and the electron frequency of 30 kHz (optimal conditions to effective plasma). Then, the effect of cold atmospheric jet plasma (argon) on gas was investigated in 30, 60 and 360 seconds (gas flow rate of 6 liters per minute).
The results showed us that the cold atmospheric pressure plasma method effectively production pure mycotoxins. The rate of various mycotoxins destruction varies according to their structure. It was also shown in the study that the combination of mycotoxins in foods would reduce the plasma's effect, but not prevent them from degrading. Finally, in this study, descendants in all samples were performed successfully.
The atmospheric pressure plasma method has a natural biodegradation effect and has the least negative effect on food products in comparison with conventional methods of disinfection of food products in addition to the detoxification characteristics.- انتشار مقاله: 24-06-1396
- نویسندگان: Narges Vaseghi,Mansour Bayat,Arash Chaichi Nosrati,Mahmood Ghorannevis,Jamal Hashemi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,Quantitative PCR,Virulence factors,Microwaves,Alpha-INT1 protein
- چکیده:
- چکیده انگلیسی: To date, registered users of mobile phone communication network exceeded from total numbers of the world population, while a little knowledge of the biological effects of, 900-1800 MHz microwave radiation, originating from the handsets or the base transceiver stations, have been released. The current study was designed for evaluation of 900-MHz radiation effects on Candida albicans proliferation, adherence and alpha-Int1 gene expression.
Candida albicans (ATCC:10231) grown in Yeast Peptone Dextrose (YPD) broth was distributed into five tubes (5 ml, 10^6 cells/ml) and exposed to 900 MHz GSM radiation for 6, 12, 18 and 24 hours, while the fifth tube was kept far from the radiation. Cell densities at 0, 6, 12, 18 and 24 hours were assayed (using turbidimetry in 600 nm). Equal cell densities (2.5 x 10^6 cells/ml, 200 ul) from exposed and unexposed yeasts were transferred into 96 well plates and incubated for 4 hours, in order to biofilm formation by the yeast. Yeast densities in biofilm network were assayed using the MTT method. Abundance of alpha-int1 mRNA was also estimated in the five yeast samples using q-RT-PCR method.
Microwave exposure led to increased proliferation rate and increased biofilm formation by the yeast and the effect was prominent in 18 hours exposed samples. Quantitative RT-PCR results showed significantly increased levels of the alpha-int1 mRNA in microwave exposed yeasts.
The significant increases in the yeast proliferation and biofilm formation after exposure to 900 MHz GSM radiation are partly mediated by changes in alpha-int1 protein expression.- انتشار مقاله: 26-07-1394
- نویسندگان: Ariyo Shahin-Jafari,Mansour Bayat,Mohammad Hassan Shahhosseini,Parviz Tajik,Shahla Roudbar-Mohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,MIC,Eucalyptus essence,Lavandula essence
- چکیده:
- چکیده انگلیسی: Candida albicans is the most common and important factor causing candidiasis infections in human. As fungi are resistant to anti-fungal agents, and also with respect to the restrictions in treatment of fungal diseases such as their high costs and side effects, study of the combination of herbal medicine is truly required. The aim of the present study was to investigate the effects of herbal essences of eucalyptus and lavandula on the growth of clinical Candida albicans strains which have been separated from mucosal, cutaneous and visceral infections, and compared to those Candida albicans which are resistant and sensitive to caspofungin. The present study has been carried out on 41 Candida albicans taken from patients suffering from mucosal, cutaneous and visceral candidiasis. The degree of samples’ sensitivity to eucalyptus and lavandula were determined through applying broth microdilution method. In the present study, the MIC (minimum inhibitory concentration) of eucalyptus against Candida albicans was determined at 4-1024µl/ml, in which the most growth fluctuations were seen in the densities of 512 and 128 µl/ml. Moreover, the MIC of lavandula was determined at 1-256 µl/ml, and the most fluctuations in its growth were in the density of 32 and 16 µl/ml. Eucalyptus and lavandula essences have a very good anti-fungal effect against Candida albicans. In the present study, lavandula essence proved better anti-candidiasis effects while no considerable difference was seen between the strains sensitive and resistant to caspofungin in its MIC. In higher densities, eucalyptus essence prevents strains from growing. Therefore, it is recommended to investigate the effects of these essences on other pathogenic fungi and bacteria. It is also recommended to evaluate the use of combinations of two or more essences for curing infections or preventing their reoccurrence.
- انتشار مقاله: 01-03-1391
- نویسندگان: Leila Fozouni,Mansour Bayat,Shahla Roudbar Mohammadi,Mohammad Hassan Shahhosseiny
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Aspergillus species,Citrinin,Toxigenic activity,Cell extracts,Food and Feed
- چکیده:
- چکیده انگلیسی: Objective: Citrinin is a mycotoxin produced by several species of the genera Aspergillus and Penicillium and occurs mainly in food products and animal feed. Some scientific reports show a link between Citrinin and nephrotoxic and possibly a carcinogenic effect for humans. Methods: Samples collected by settling plates,in northern Iran and pure culture isolation performed till the toxin measurement to be done in cell extracts (biomass) which prepared by merging culture in separated prepared culture media incubation. The amount of toxin measured by extracting solutions (50% Acetone, 25% Alcohol Methylic,25% Alcohol Ethylic and PBS washing proposed) using Direct Competitive ELISA. Results: In conducted research, the relative distribution per obtained Aspergillus species isolates for biomass preparation showed that the most frequent were A.ostianus, A.fumigatus,A.niveus,A.niger,A.awamori and A.parasiticus respectively with a prevalence (28.6%), according to averaged Citrinin, indicated that the potent species was A.niger (2009.3ppb) whereas the lowest observed by A.wentii (18.46 ppb).According to maximum Citrinin limits (200ppb) in food products and animal feed including especially related to the Aspergilli and Penicilli, as the result of our measurements and the performed statistical analysis , the maximum amount of Citrinin in the cell extracts, respectively were determined (2009.3ppb) and always produced by A.niger , that were too much more than the universal and local standards allowance enable us to introduce it for all biotechnologic or toxicologic research mentions eventhought as a scientific opinion on the risks for public and animal health related to the presence of Citrinin in food and feed at the targeted geographic area.
- انتشار مقاله: 23-06-1393
- نویسندگان: Pouya Nejati,Arash Chaychi Nosrati,Mansour Bayat,Omid Lakzaie Azar
- مشاهده
- جایگاه : پژوهشی
- مجله: Current Medical Mycology
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR-RFLP,Mucorales,Mucor,Rhizopus,Lichtheimia,Rhizomucor,Mortierella
- چکیده:
- چکیده انگلیسی: Background and Purpose: Soil is the main habitat of saprophytic and pathogenic fungi. Mucormycetes are one of the most parts of soil fungi and certain members are among opportunistic fungi and can cause systemic fungal infections in immunocompromised patients. The majority of human and animal infections are caused by members of the genera Rhizopus, Mucor, Rhizomucor, Lichtheimia (Absidia), Cunninghamella and Mortierella.
The objective of this research was to isolate and identify the main genera of Zygomycetes, using molecular assay and morphological features.
Materials and Methods: A total of 340 soil samples were collected from different sites of seven public parks and 14 municipality districts in Isfahan. All samples were cultured on appropriate media and incubated at 27° C for 2 to 4 days, and then examined daily for visible fungal growth. PCR-RFLP method and macroscopic, microscopic and physiological characteristics were applied to identify fungal colonies.
Results: Four hundred pure colonies belonging to six genera of Zygomycetes including Lichtheimia, Rhizopus, Rhizomucor, Mucor, Cunninghamella and Mortierella were identified. The genus Rhizopus (35.5%) was the most frequent isolate, followed by Mucor (32.25%) and Rhizomucor (27.5%).
Conclusion: These finding may help us to understand about the importance of opportunistic fungi in public areas and the risk of exposure with immunocompromised persons.- انتشار مقاله: 18-04-1398
- نویسندگان: Ardeshir Ziaee,Mohammadali Zia,Mansour Bayat,Jamal Hashemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Environmental Energy and Economic Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Delphi method,Environmental Management Performance,Urban Health Centers
- چکیده:
- چکیده انگلیسی: Medical centers (that is, hospitals and clinics) in metropolitan areas produce substantial amounts of hazardous waste in both solid and liquid (wastewater) forms. Unless medical centers manage their waste with appropriate care, the significant hazards posed to urban residents and areas far outweigh the benefits of their services. Therefore, comprehensive environmental management programs need to be developed in order to minimize the associated impacts and improve environmental performance. In addition to identifying the environmental consequences, developing a comprehensive performance assessment program requires a deep understanding of the factors involved and their corresponding shares. Thus, the aim of this paper is to model environmental performance assessment in urban medical centers. Firstly, relevant criteria, sub-criteria and indicators were identified using the Delphi method. Next, Fuzzy Analytical Hierarchy Process (AHP) was applied to rank the factors and a balanced scoring was established to model the environmental performance assessment in medical centers. The adequate consistency index confirms Fuzzy AHP accuracy and consequently, the scores. The model was applied in Sarem Specialized Hospital in Tehran, Iran. The results indicate that the hospital has good environmental performance. The results show that the waste disinfection indicator, was rated the highest and most important index with a score of 84.The other indicators were ranked in terms of treated sewage and source separation of waste and application of mechanisms to control water consumption respectively with scores of 38, 26.5 and 25.
- انتشار مقاله: 19-05-1398
- نویسندگان: Nazak Shahbod,Mansour Bayat,Nabiollah Mansouri,Jafar Nouri,Jamal Ghodousi
- مشاهده