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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: HTLV-1,luciferase,Reporter gene,Biological Assay,Drug screening,Hut102
- چکیده:
- چکیده انگلیسی: Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously.
Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids.The first reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell. In order to show its functionality, the effect of the drug mix on HTLV-1 was assayed by this system and was compared to the results obtained by other methods.
Results: HTLV-1 reporter cell line was found to produce high level of luciferase when co-cultured with MT-2 and Hut-102 cells but not with Jurkat cell. Moreover, the combination therapy against HTLV-1 can reduce luciferase expression of the cell when co-cultured with MT-2 and Hut-102 comparable to the ELISA (R=0.932, P-value =0.002). In addition, the results revealed the superiority of the present system over the molecular methods.
Conclusion: The results demonstrated that the biological assay system is a beneficial tool for the medium-throughput anti-HTLV-1 drug screening and inhibitory effect.- انتشار مقاله: 04-07-1396
- نویسندگان: Mojtaba Fattahi Abdizadeh,Manoochehr Makvandi,Alireza Samarbafzadeh,Kayhan Azadmanesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Polymerase Chain Reaction,JC Virus,BK Virus
- چکیده:
- چکیده انگلیسی: JC virus (JCV) , and BK virus (BKV) can remain latency in kidney and excrete via urine asymptomatically. JCV has been associated with colorectal and bladder cancers. BKV has been linked with lung, pancreas, liver, urogenital tract, head and neck cancers. Therefore, the frequency of JCV DNA and BKV DNA are essential to evaluate in urine samples of healthy individuals. Materials and Methods: Hundred sixty four urine samples were collected from healthy subjects [96 females and 68 males]. DNA was extracted and detection of JCV DNA and BKV DNA was carried out by PCR . The analysis of sequencing and construction of phylogenetic tree were performed for the samples positive for JCV DNA and BKV DNA. Results: Ten (6.09%) urine samples [5/96(5.2%) females and 5/68( 8.82) males] were tested positive for JCV DNA (P= 0.814). The results of sequencing and phylogenetic tree showed the isolated JCV DNA were cluster with 3A genotype. 21/164 (12.8%) samples were tested positive for BKV DNA [11/96(11.45%) females and males 10/68(14.7%)] ( P= 0.63). The results of sequencing and phylogenetic tree showed that the isolated BKV was cluster with genotype III. Conclusion: In the present study 6.09% and 12.8% of the healthy individuals showed positive for JCV DNA (genotype 3A) and BKV DNA(genotype III) respectively. With regard to life threating diseases by BKV and JCV in immunocomprsied patients , the screening BKV DNA and JCV DNA should be implemented for patients with cancer /autoimmune diseases /organ recipient/ multiple sclerosis (MS), prior to immunosuppression therapy or immunomodulatory agents treatment.
- انتشار مقاله: 26-09-1398
- نویسندگان: Lila Karimi Dehcheshmeh,Manoochehr Makvandi,Ali Timori
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Polymerase Chain Reaction,Ductal carcinoma,Human Cytomegalovirus
- چکیده:
- چکیده انگلیسی: Breast cancer is the most common cause of death among women worldwide. Although there are many known risk
factors in breast cancer development, infectious diseases have appeared as one of the important key to contribute to
carcinogenesis formation. The effects of Human Cytomegalovirus (HCMV) on women with breast cancer has been
recently studied and reported. To contribute to this research trend, this study was conducted to evaluate the association
between HCMV and the women with breast cancer. Objective: This experiment aimed to evaluate HCMV DNA in women
with breast cancer in Ahvaz city, Iran. Materials and Methods: A total of 37 formalin fixed paraffin embedded tissues of
the patients with ductal breast carcinoma and 35 paraffin embedded tissues of the patients with fibro adenoma as control
group were collected. The deparaffinization of all the samples were carried out and the DNA was extracted. Initially,
the PCR test was carried out to detect beta –globulin DNA as an internal control. For those samples positive for beta
–globulin DNA, Polymerase Chain reaction (PCR) was used to detect HCMV for the tests and control samples. Results:
Among 37 ductal breast carcinoma, 20 (54.04%) cases were proved positive for HCMV DNA by PCR. While among
the 35 control group (fibroadenoma), 10 (28.57%) cases were positive for HCMV DNA (P >0.028). The prevalences of
HCMV DNA among the age groups 30-39, 40-49 and >50 years were 7 (72.22%), 9 (69.23%), 4 (57.14%), respectively
(P=0.066). A high frequency of HCMV DNA was detected in tumor grade III, 13/18 (58.33%) compared with tumor
grade II, 7/19 (36.84%) (p=0.044). A high frequency of 16/24 (66.66%) of HCMV DNA was found in invasive ductal
breast cancer compared with 4/13 (30.76%) HCMV DNA in situ (P<0.028). Conclusion: A high prevalence of 54.05%
HCMV was found among the patients with ductal carcinoma. The percentages of the high prevalence of HCMV among
age group (40-49) years, tumors grades, and invasive stage were (69.23%), (58.33%), (66.66%), respectively. Further
study of HCMV in the latency phase in patients with ductal carcinoma would be necessary to extend our knowledge.- انتشار مقاله: 22-06-1397
- نویسندگان: Peyman Sepahvand,Manoochehr Makvandi,Alireza Samarbafzadeh,Abdulhasan Talaei-Zadeh,Nastaran Ranjbari,Nilofar Nisi,Azarakhsh Azaran,Shahram Jalilian,Roya Pirmoradi,Kimia Makvandi,Kambiz Ahmadi Angali
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: JC Virus,colorectal adenocarcinoma,Polymerase Chine Reaction
- چکیده:
- چکیده انگلیسی: Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitous
Polyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV have
been reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon and
rectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancer
type Adenocarcinoma. Material and Methods: A total of 120 formalin-fixed paraffin-embedded tissue blocks samples
were collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50%
males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR was
carried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR products
of 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determine
homology identity with other JCV. Results: 4/40(10%) samples of test group and 10/80 (12.5%) of control samples
were positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female
(P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group (0%) (p= 0.29). Conclusion: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors
(p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCV
DNA was among 10% cases of age group >50 years and 0% of age group protein expression might explain the increased risk of colorectal cancer and requires further investigation.- انتشار مقاله: 08-07-1397
- نویسندگان: Azadeh Haghi Navand,Ali Teimoori,Manoochehr Makvandi,Nilofar Nisi,Seyed Saeid Seyedian,Nastaran Ranjbari,Kambiz Ahmadi Angali,Hadis Keyani,Maryam Tabasi,Keyvan Pourjabari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Epstein Barr virus,Ductal carcinoma,Nested PCR
- چکیده:
- چکیده انگلیسی: Background: Ductal carcinoma is one of the most common breast cancer (BrC) among the women in the world.
Several factors may involve in establishment of breast cancer. The role of viral infections have been investigated in
BrC, Among them the association of Epstein Barr virus have been reported in the patients with breast cancer type
ductal carcinoma. Thus this study was conducted to evaluate the rate of Epstein Barr virus in women with breast cancer
type ductal carcinoma. Material and methods: A total of 72 formalin-fixed paraffin-embedded tissue blocks samples
were collected from 37 (51.38%) women with breast cancer type ductal carcinoma and 35 (48.61%) samples of breast
with fibro adenoma as control group. The DNA was extracted for all the samples. The detection of EBNA 3C EBV
DNA was done by nested PCR. The results of positive were sequenced to confirm PCR product and determine EBV
genotypes. Results: About 10/37 (27.02%) samples of ductal breast carcinoma were showed positive for EBNA 3C
EBV DNA while 4/35 (11.42%) of fibro adenoma were positive for EBNA 3C EBV DNA (p= 0.095). Randomly 7
PCR products were sequenced and the results of sequencing EBNA 3C shows, the detected EBVDNA were type 1
EBV type. Conclusion: This study shows high prevalence of 27.02% EBV DNA type 1 was found in formalin-fixed
paraffin-embedded tissue of Patients with ductal breast carcinoma. The outcomes of this study suggesting that EBV
might have a significant role in breast cancer in Ahvaz city, south west region of Iran. However the expression of EBV
oncoproteins ,EBNA1, LMP1, and LMP2 require to be determined with ductal carcinoma cells. About 72.97% breast
samples showed negative for EBVDNA. The role other viruses including Human cytomegalovirus, papilloma viruses
and Merkel viruses are required to be investigated in further studies.- انتشار مقاله: 12-06-1396
- نویسندگان: Chia Sharifpour,Manoochehr Makvandi,Alireza Samabafzadeh,Abdolhassan Talaei-Zadeh,Nastaran Ranjbari,Nilofar Nisi,Azarakh Azaran,Shahram Jalilian,Mehran Varnaseri,Roya Pirmoradi,Kambiz Ahmadi Angali
- مشاهده