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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Gold nanoparticle,Nitric oxide,Multiple Sclerosis,L-arginine,Demyelination,corpus callosum
- چکیده:
- چکیده انگلیسی: Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs).
Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA).
Results: L-arginine significantly induced (p< 0.05) a reduction in the fiber density while the neurons increased (p< 0.05). Single GNPs reduced (p< 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons.
Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.- انتشار مقاله: 25-03-1398
- نویسندگان: Mahjabin Khosravi,Manizheh Karami,Mohammadreza Jalali Nadoushan,Abazar Hajnorouzi
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Gold nanoparticle,Nitric oxide,Multiple Sclerosis,L-arginine,Demyelination,corpus callosum
- چکیده:
- چکیده انگلیسی: Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs).
Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA).
Results: L-arginine significantly induced (p< 0.05) a reduction in the fiber density while the neurons increased (p< 0.05). Single GNPs reduced (p< 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons.
Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.- انتشار مقاله: 25-03-1398
- نویسندگان: Mahjabin Khosravi,Manizheh Karami,Mohammadreza Jalali Nadoushan,Abazar Hajnorouzi
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Research Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,naloxone,silver nanoparticles,c-Fos,Central amygdala
- چکیده:
- چکیده انگلیسی: Abstract
Background: Silver nanoparticles (Ag-NPs) that are used daily in care service can enter the body and create free radicals. Despite the toxicity at high concentrations, these particles are non-toxic and useful at low concentrations. Thus, we investigated the effectiveness of nontoxic Ag-NPs to interfere with the aversive effect of naloxone (NLX) and low expression of c-Fos during testing of morphine-induced conditioned place preference (CPP) in rats. Methods: The Wistar rats (weighing 300-350 g) were cannulated bilaterally by stereotaxic apparatus for the CeA (AP= –2.12 mm; L= ±4.1 mm; V= 7.8 mm). CPP was conducted via a three-phase unbiased procedure. Morphine (0.5-7.5 mg/kg) was injected subcutaneously (sc) during the conditioning phase. NLX (0.4 µg/rat) was given, intra-CeA, 10 min before the test. Ag-NPs (0.01 µg/rat) was administered prior to the antagonist. The control group received saline (1 µL/rat, intra-CeA). c-Fos expression was quantified immunohistochemically in rats subsequent the injections: Results: The CeA and hippocampal cornu ammonis 1 (CA1) of rats that treated by NLX showed low c-Fos protein levels during testing, whereas levels of protein were high in the brains of morphine conditioned rats. Interestingly, both areas (CeA and CA1) showed similar increases in protein levels when the injection of NLX was combined with the Ag-NPs. However, these regions were not significantly different in the single Ag-NPs receiving and control groups. Conclusions: This indicates that the two regions interact with each other when NLX is injected and that in presence of Ag-NPs the protein levels are elevated in the regions.- انتشار مقاله: 13-05-1399
- نویسندگان: Mahnaz Rahimpour,Manizheh Karami,Ali Haeri Rohani
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Basic and Clinical Pathophysiology
- نوع مقاله: Journal Article
- کلمات کلیدی: colchicine,Wistar rat,Generalized epilepsy,Dorsal striatum,Identified doses
- چکیده:
- چکیده انگلیسی: Background and Objective: Despite the massive use of costly models, our understanding of epilepsy is insignificant. We aimed to create the cost-beneficial and applicable generalized epilepsy (GE) in rats by injection of identified doses of colchicine (IDC) into the dorsal striatum of rat's brain.
Materials and Methods: 24 male Wistar rats (290-350 g) under deep anesthesia were equipped with guide cannulae at the dorsal striatum (AP: 0.48 mm; L: 3 mm; V: 3.6). The injection cannula attached with the polyethylene tubing to the 5-µL Hamilton syringe guided daily IDC (9-25 μg,) into the dorsal striatum of rat's brain during four consecutive days. The control group only received the saline solution. At the end of the injection, the animal's behavior was investigated. Finally, all the brains were collected in 10% formalin solution and dissected out to include the striatal regions. The samples were cut coronally into 3-4 µm thick slices, embedded in paraffin, and processed by the step section technique. The slices were then stained with Hematoxylin-Eosin method and checked under the light photomicroscope for correct placement of injections as well as the possibility of lesions. The data were compared between vehicle and experimental groups by analysis of variance (ANOVA).
Results: The findings show that GE occurs in animals receiving colchicine without significant devastation effect in the injection site in the brain of rat.
Conclusion: The plant-derived alkaloid, the colchicine, aside depolymerizing of tubulin, may have an inductive effect on epilepsy in the complementary motor striatal region of the rat's brain- انتشار مقاله: 17-02-1399
- نویسندگان: Atiyeh Nejadebrahim,Manizheh Karami,Mehrdad Roghani,Zahra Fakhroleslam
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Basic and Clinical Pathophysiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Ethanol,acquisition,CA1,Novelty-seeking behavior,Place neuron
- چکیده:
- چکیده انگلیسی: Background and Objective: Ethanol has undesirable effects on memory and synaptic communication. However, its impact on the learned spatial memory is unclear. We investigated the damaging effects of ethanol on place neurons of rat’s hippocampal CA1.
Materials and Methods: Sixty four male Wistar rats (250 g) were administered high (1-8 g/kg) or low (0.05-0.1 g/kg) doses of ethanol intraperitoneally (i.p.) and tested (10 min) for the novelty-seeking behavior using the place conditioning box. Sniffing, rearing, grooming, and compartment entering was compared between the first and the last stage, during which the animals had accessibility to the whole device. During the acquisition phase, the rats daily received ethanol (i.p.) and confined in one side of the device for 40 min. The control group solely received saline (1 ml/kg, i.p.). The achievements were analyzed by ANOVA under α = 0.05.
Results: The ethanol-acquired animals with the high doses did not recall the information of the familiarization day and displayed a high tendency toward the non-confined side of the box. The rats also showed a reduction in place neuron synaptic strands.
Conclusion: Ethanol disrupts spatial memory and also diminishes CA1 place neuron’s fibers.- انتشار مقاله: 12-11-1398
- نویسندگان: Samira Geravand,Manizheh Karami
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Basic and Clinical Pathophysiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,Male Rat,Infertility,Dopamine,Sulpiride
- چکیده:
- چکیده انگلیسی: Introduction & Objective: Male fertility depends on the proper function of a complex system of organs. Harmful effects of morphine on male reproduction and fertility are well documented. Dopamine facilitates sexual behavior in different animal species. The antagonizing of dopamine D2 receptors with sulpiride was aimed to investigate the interaction of dopaminergic system with morphine-induced infertility.
Materials and Methods: A total of 48 adult male Wistar rats with a weight range of (220-320 g) were studied. In the first category, morphine (5 mg/kg) was injected i.p. The next groups received sulpiride (1-4 mg / kg) alone and prior (20 min) to morphine (5 mg / kg). The control group received only saline (1 ml / kg). All rats were sacrificed under deep anesthesia a week after. Their testicles were collected for examination. LH, FSH and testosterone were measured in the sera. Data were analyzed by ANOVA.
Results: Significant decreases in dimensions of testicles were observed in groups’ rats that receiving morphine or sulpiride prior to the morphine. Seminiferous tube destruction was observed in morphine and in group that receiving sulpiride prior to morphine; destruction was increased with increasing dose of sulpiride. Significant decrease of serum testosterone level was observed in rats receiving morphine and high dosage of sulpiride. Significant increase in serum testosterone level was observed in group receiving sulpiride prior to morphine.
Conclusion: It seems that dopamine has an interacting effect with morphine on induction of male infertility and its mechanism is probably carried out through dopamine D2 receptors.- انتشار مقاله: 14-06-1398
- نویسندگان: Mahdiyeh Jafarpour Fard,Manizheh Karami,Mohammadreza Jalali Nadoushan
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Basic and Clinical Pathophysiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Ethanol,Rat,Novelty seeking behavior,Information recall
- چکیده:
- چکیده انگلیسی: Background and Objective: Identifying the details of the injurious effects of alcohol seems to be decisive in nowadays with growing abuse of the substance. This study mainly aimed to assess the adverse effects of ethanol on the recalling of information in the Wistar rats.
Materials and Methods: Male Wistar rats (Pasteur Institute of Iran) were evaluated using the novelty seeking behavior based on the place conditioning task. The animals in the conditioning phase were confined in only one side of the equipment. They (n=8 per group) were intraperitoneally injected ethanol at high (1-8 g/kg) or low (0.1-0.8 g/kg) concentrations prior to the testing. The control group solely received saline solution (1 ml/kg, i.p.) in the testing day. The liver samples of the experimental animals were examined to provide the setback systemic injury evidence for the ethanol.
Results: Based on the findings, the ethanol treated rats did not remember the previous data and showed more interest to the novel side (pConclusion: Recalling of the facts need permanent comparisons between the receiving reminiscences from environ with the information which already exist in the memory boxes. This study may show the prompt troubling influence of ethanol on the memory retrieval along with the long-lasting systemic hazard of the biggest gland.- انتشار مقاله: 27-03-1394
- نویسندگان: Samira Geravand,Manizheh Karami,MohammadReza Jalali Nadoushan
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Basic and Clinical Pathophysiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Morphine,Ovary,Polycystic
- چکیده:
- چکیده انگلیسی: Background and Objective: The effect of opioids on the reproductive system has been studied as an attractive research field. In this study, the effect of morphine as an analgesic opioid was evaluated on the ovarian polycystic induction in the rat.
Materials & Methods: In this study, 24 female virgin Wistar rats weighing 200-250 g were injected with morphine (1-10 mg/kg/day) for 9 days. Control group received normal saline (1 ml/kg, i.p.) throughout the experimental period. After completion of injection period, all animals underwent surgery to collect the ovaries. After biometrical measurements, the ovaries specimens were fixed in the formalin to use in future study.
Results: The ovaries from morphine-injected rats had thick-wall follicular cysts whilst the control samples showed the Graafian follicles. In addition, the higher doses of morphine (10 mg/kg) led to much thicker wall cysts than the lower doses (1 mg/kg). However, the experimental samples did not show a significant difference versus control in view of their size.
Conclusion: The enhanced irregularity in the opioid system activity has been reported in polycystic ovary syndrome (PCOS). Based on these findings, the induction of connective disturbance between the opioid and the reproductive systems due to chronic use of the morphine may crucially play a role in ovarian polycystic induction in the rat.- انتشار مقاله: 22-02-1393
- نویسندگان: Manizheh Karami,Maryam Darban Fooladi,Mohammad Reza Jalali Nadoushan,Fatemeh Lakzaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Amygdala,morphine dependence,naloxone,Nitric oxide
- چکیده:
- چکیده انگلیسی: Objective(s) Single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence. Materials and Methods Conditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Nitric oxide agents were microinjected into the central amygdala prior to naloxone-paired place conditioning testing. Results The results showed that morphine produced a significant dose-dependent place preference in animals. Naloxone (0.1-0.4 mg/kg, i.p.) injections pre-testing of the response to morphine (7.5 mg/kg, s.c.) caused a significant aversion at the higher doses (0.4 mg/kg, i.p.). This response was reversed by microinjection of L-arginine (0.3-3 μg/rat, intra-central amygdala) prior to naloxone on the day of the testing. The response to L-arginine was blocked by pre-injection of NG-nitro-L-arginine methyl ester (L-NAME) (intra-central amygdala). Conclusion A single injection of naloxone on the test day of morphine place conditioning may simply reveal the occurrence of morphine dependence in rats, and that the nitric oxide in the central amygdala most likely plays a key role in this phenomenon.
- انتشار مقاله: 30-06-1394
- نویسندگان: Mahnaz Rahimpour,Manizheh Karami,Sara Karimi,Abbas Haghparast,Mohammad Reza Jalali,Farzaneh Sabouni
- مشاهده