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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Health Sciences and Surveillance System
- نوع مقاله: Journal Article
- کلمات کلیدی: Students,mental health,Premenstrual Syndrome
- چکیده:
- چکیده انگلیسی: Background: Premenstrual syndrome is considered as one of the most common psychiatric disorders that greatly disrupts women's life.Objective: The aim of this study was to investigate the correlation between mental health and premenstrual syndrome among female students of Shiraz University of Medical Sciences.Materials and Methods: This study was conducted in 2016 on a total of 168 students residing in dormitories of Shiraz University of Medical Sciences who were selected by block randomization method. Data collection tools included demographic data, PSST & GHQ questionnaire. Data were conducted throughSPSS software, version 22, using descriptive tests and Spearman correlation coefficient.Results: Spearman correlation coefficient showed a significant correlation (p<0.001) between premenstrual syndrome score, mental health and all its dimensions except for social interactions (P = 0.525). Moreover, anxiety and sleep disorders with a frequency of 51.2% were the most common mental disorder among the students.Conclusion: Our study showed that there was a correlation between premenstrual syndrome score and mental health in girls. Therefore, necessary measures should be taken by counseling centers in schools and universities to improve the mental health of people with premenstrual syndrome who have a higher risk of psychological and physical disorders.
- انتشار مقاله: 20-09-1396
- نویسندگان: Marzieh Akbarzadeh,Naval Heydari,Malihe Abootalebi,Fatemeh Ghodrati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: MHT,Carbapenem resistance,Klebsiella pneumoniae,bla-KPC gene
- چکیده:
- چکیده انگلیسی: Background and Objective: The production of carbapenemases especially Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance in isolated Enterobacteriaceae such as K. pneumoniae. The purpose of this study was detected of the carbapenemase producer K. pneumoniae strains with phenotypic and genotypic methods. Method: Out of 800 strains, 270 K. pneumoniae strains (33.7%), were obtained. Antibiotic susceptibility test was performed by disk diffusion method in accordance with CLSI guidelines. Carbapenem resistant strains were identified by the Modified Hodge Test based on CLSI instruction and PCR for surveying the presence of bla-KPC gene. Results: A total 270 K. pneumoniae strains were collected. Antibiotic susceptibility test results showed the highest and lowest resistance was related to piperacillin (60.6%) and carbapenems (14.6%) respectively. 80.5% (33 of 41) isolates were positive by MHT, but all of them (100%) were negative for amplification of the bla-KPC gene in the PCR method. Conclusion: The MHT was an appropriate method for approving carbapenemase production. Moreover, a laboratory could accept the carbapenemase production with PCR method for the bla-KPC gene, which has the additional profit of validating which KPC is present. How to cite this article: Bina M, Pournajaf A, Mirkalantari S, Talebi M, Irajian G. Detection of the Klebsiella pneumoniae carbapenemase (KPC) in K. pneumoniae Isolated from the Clinical Samples by the Phenotypic and Genotypic Methods. Iran J Pathol. 2015;10(3):199-205.
- انتشار مقاله: 21-11-1392
- نویسندگان: Masoume Bina,Abazar Pournajaf,Shiva Mirkalantari,Malihe Talebi,Gholamreza Irajian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Tandem repeats,Bacteroides fragilis,MLVA,PCR amplification,Typing
- چکیده:
- چکیده انگلیسی: Objective(s): Human gastrointestinal tract harbors a variety of bacteria with vital roles in human health. Bacteroides fragilis is considered one of the dominant constituents of gut microflora which can act as an opportunistic pathogen leading to various diseases, including colon cancer, diarrhea, uterine and intrathecal abscesses, septicemia, and pelvic inflammation. In this study, multiple locus variable number of tandem repeats analysis (MLVA) was performed to genetically differentiate 50 B. fragilis isolates.
Materials and Methods: Eight suitable tandem repeats (TRs) were selected by bioinformatics tools and were then subjected to PCR amplification using specific primers. Finally, MLVA profiles were clustered using BioNumerics 7.6 software package.
Results: All VNTR loci were detected in all isolates using the PCR method. Overall, B. fragilis isolates were differentiated into 27 distinct MLVA types. The highest diversity index was allocated to TR1, TR2, TR5, TR6, and TR8; with this taken into account, strain type 14 was the most prevalent with 12 strains belonging to this type. Clustering revealed three major clusters of A, B, and C. With regards to the pathogenicity of B. fragilis and the outcomes of infections related to this microorganism, it is imperative to study this microorganism isolated from both patients and healthy individuals.
Conclusion: This study aimed at evaluating the efficiency of MLVA for the genetic differentiation of B. fragilis. The results of this study indicate the promising efficiency of MLVA typing for cluster detection of this bacterium.- انتشار مقاله: 03-08-1397
- نویسندگان: Niloofar Khodaei,Behrooz Sadeghi Kalani,Maryam Zamani,Rokhsareh Mohammadzadeh,Malihe Talebi,Tahmine Narimani,Negar Narimisa,Faramarz Masjedian Jazi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Tandem repeats,Bacteroides fragilis,MLVA,PCR amplification,Typing
- چکیده:
- چکیده انگلیسی: Objective(s): Human gastrointestinal tract harbors a variety of bacteria with vital roles in human health. Bacteroides fragilis is considered one of the dominant constituents of gut microflora which can act as an opportunistic pathogen leading to various diseases, including colon cancer, diarrhea, uterine and intrathecal abscesses, septicemia, and pelvic inflammation. In this study, multiple locus variable number of tandem repeats analysis (MLVA) was performed to genetically differentiate 50 B. fragilis isolates.
Materials and Methods: Eight suitable tandem repeats (TRs) were selected by bioinformatics tools and were then subjected to PCR amplification using specific primers. Finally, MLVA profiles were clustered using BioNumerics 7.6 software package.
Results: All VNTR loci were detected in all isolates using the PCR method. Overall, B. fragilis isolates were differentiated into 27 distinct MLVA types. The highest diversity index was allocated to TR1, TR2, TR5, TR6, and TR8; with this taken into account, strain type 14 was the most prevalent with 12 strains belonging to this type. Clustering revealed three major clusters of A, B, and C. With regards to the pathogenicity of B. fragilis and the outcomes of infections related to this microorganism, it is imperative to study this microorganism isolated from both patients and healthy individuals.
Conclusion: This study aimed at evaluating the efficiency of MLVA for the genetic differentiation of B. fragilis. The results of this study indicate the promising efficiency of MLVA typing for cluster detection of this bacterium.- انتشار مقاله: 03-08-1397
- نویسندگان: Niloofar Khodaei,Behrooz Sadeghi Kalani,Maryam Zamani,Rokhsareh Mohammadzadeh,Malihe Talebi,Tahmine Narimani,Negar Narimisa,Faramarz Masjedian Jazi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Real-time PCR,bla OXA-23,bla OXA-48,E-test,MHT
- چکیده:
- چکیده انگلیسی: Background:
The prevalence of carbapenem-resistant Enterobacteriaceae, especially Klebsiella pneumoniae carbapenemase (KPC), has been recently reported worldwide. Therefore, there is an indispensable need for precise and rapid detection of these carbapenemases.
Objectives:
This study aimed to propose an accurate and rapid method for detecting K. pneumoniae carbapenemase genes from clinical samples, using reverse transcription-polymerase chain reaction (RT-PCR) and to evaluate the expression of these genes in the presence of β-lactam antibiotic by real-time PCR assay.
Methods:
One hundred and eighty-one K. pneumoniae strains were collected from patients presenting to Firoozgar Hospital of Tehran, Iran. The strains were tested using the disk diffusion method, the modified Hodge test (MHT), and E-test minimum inhibitory concentration (MIC). Next, reverse transcription-PCR method was applied for the identification of bla OXA-23 and bla OXA-48 genes. Finally, expression of genes was measured by real-time PCR assay in the presence and absence of β-lactam antibiotic.
Results:
Phenotypic testing also showed a high level of antibiotic resistance, while the genotypic methods indicated the presence and expression of carbapenemase genes.
Conclusions:
The findings suggest revisions in the current antibiotic therapy protocols, considering the high expression level of resistant carbapenemases to K. pneumoniae strains.- انتشار مقاله: 22-03-1398
- نویسندگان: Narjes Mohammadi Bandari,Hossein Keyvani,Mohsen Zargar,Malihe Talebi,Mohammad Reza Zolfaghari
- مشاهده